ERG Antibody (EPR3864(2))

Product Discontinued

ERG Antibody (EPR3864(2)) Summary

• Immunogen: A synthetic peptide corresponding to residues on the C-terminus in human ERG was used as an immunogen.
• Specificity: This antibody does not cross-react with Fli1.
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ERG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-250
  • Immunoprecipitation 1:10-100
  • Western Blot 1:1000-10000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.05% BSA and 50% Glycerol, pH 7.4
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for ERG Antibody (EPR3864(2))

• transcriptional regulator ERG
• 10ets-related
• Erg
• Erg-3
• p55
• TMPRSS2-ERG prostate cancer specific
• transcriptional regulator ERG
• Transforming protein ERG
• v-ets erythroblastosis virus E26 oncogene homolog (avian)
• v-ets erythroblastosis virus E26 oncogene like (avian)
• v-ets erythroblastosis virus E26 oncogene like

Background

Transcriptional Regulator ERG belongs to the ETS family that is involved in the regulation of a wide variety of cellular and developmental processes (1). This family of transcription factors contains approximately 30 members that share a highly conserved DNA-binding domain (ETS domain) (2, 3) and differs from each other in other domains (such as absence or presence of the Pointed/SAM domain) and are thus distinguished in sub-families (1). ERG belongs to the Erg/Fli-1 sub-family. It has been widely studied for its involvement in human cancers; TMPRSS2-ERG fusion, which occurs on account of translocations and interstitial deletions, is implicated in aggressive forms of prostate cancer (4, 5). ERG is linked to normal processes such as mesoderm formation and is found to form functional complexes with Jun/Fos, with the resulting ternary complexes regulating expression of genes such as metalloprotease-1 (MMP-1) and MMP-3 (1). It may also participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERG Antibody (NBP1-95857)(1)

Publication using NBP1-95857

• Masuzawa M, Mikami T, Numata Y et al. Association of D2-40 and MMP-1 expression with cyst formation in lung metastatic lesions of cutaneous angiosarcoma on the scalp: immunohistochemical analysis of 23 autopsy cases. Hum Pathol. 2013-10-11 [PMID: 24125743] (IHC-P, Human)

FAQs for ERG Antibody (NBP1-95857). (Showing 1 - 3 of 3 FAQ).

1. I am looking for an antibody that can IP Erg protein and doesn't cross react with Fli protein.
   • I have spoken to the lab and they have informed me that actually that the antibody (catalog #NBP1-95857) is confirmed not to cross react with Fli1 protein so it should be a viable choice for you in your experiments on Erg.
2. I am looking for an antibody that can IP Erg protein and doesn't cross react with Fli protein.
   • I have spoken to the lab and they have informed me that actually that the antibody is confirmed not to cross react with Fli1 protein, so it should be a viable choice for you in your experiments on Erg.
3. I need to know the exact amino acid sequence targeted by the following antibody: ERG Antibody (NBP1-95857). I at least need to know if it targets the ETS domain in ERG (aa 310-393)?
   • The exact immunogen sequence for NBP1-95857 is proprietary, but I am able to disclose the region where it is found, which is amino acids 457-486.

Bioinformatics

• Gene Symbol: ERG
• Entrez:
  • 2078(Human)
• Uniprot:
  • P11308(Human)