FANCD2 Antibody (FI-17) - BSA Free

Simple Western: FANCD2 Antibody (FI-17) [NB100-316] - Image shows a specific band for FANCD2 in 1 mg/mL of HeLa cell lysate using FANCD2 Antibody (FI-17). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: FANCD2 Antibody (FI-17) [NB100-316] - MCF7 cell line (lane 1, molecular weight: 164.1 KDa) and HeLa whole cell extract (lane 2) using FANCD2 Antibody (FI-17).

Western Blot: FANCD2 Antibody (FI-17) [NB100-316] - Expression of FANCD2 (Molecular weight: 164.1 KDa) in different cell lines. Proliferating cells were treated with 10-Gy IR. 50 ug protein was loaded each lane. FANCD2 Antibody (FI-17) at 1:1000.

• Reactivity: Hu, Ha(-), Mu(-)
• Applications: WB, Simple Western, ICC/IF, IP, ChIP
• Clone: FI-17
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

FANCD2 Antibody (FI-17) - BSA Free Summary

• Immunogen: This FANCD2 Antibody (FI-17) was developed against human FANCD2 fusion protein [Swiss-Prot: Q9BXW9]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FANCD2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunoprecipitation
  • Simple Western 1:50
  • Western Blot 1:1000 - 1:2000
• Application Notes: In Western blot, this antibody recognizes a band at ~155 and ~162 kDa (post-translationally modified form). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 150 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 164.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for FANCD2 Antibody (FI-17) - BSA Free

• DKFZp762A223
• FA4
• FACD
• FAD
• FAD2
• FA-D2
• FADFAD2
• FANCD
• FANCD2
• Fanconi anemia complementation group D2
• Fanconi anemia group D2 protein
• Fanconi anemia, complementation group D2
• FLJ23826
• FPN1
• HFE4
• IREG1
• Protein FACD2
• SLC11A3

Background

Fanconi Anemia (FA) is an autosomal-recessive cancer-prone disorder characterized by congenital defects, progressive bone marrow failure, increased chromosomal breakage, defective DNA repair and cellular hypersensitivity to mitomycin C. Fanconi Anemia Complementation Group D (FANCD) is comprised of two separate proteins, FANCD1/BRCA2 and FANCD2. LYRIC/MTDH, an RNA binding protein, regulates expression of FANCD2 and FANCD1 (1). FANCD2 is mapped to chromosome 3p25.3, has a theoretical molecular weight of 166 kDa and is localized in the nucleus. FANCD2 is involved in the regulation of DNA-binding transcription factor activity and DNA stability through accurate and efficient pairing of homologs during meiosis to promote repair of double-strand DNA breaks (2). Ubiquitination is required for FANCD2 to bind to chromatin. In response to DNA damage, FANCD2 is monoubiquinated to result in colocalization with other proteins (BRCA1 and BRCA2) involved in homology-directed DNA repair in the nucleus and is deubiquitinated upon DNA repair completion.

References

1. Bi, J., Areecheewakul, S., Li, Y., Yang, S., Zhang, Y., Ebeid, K., . . . Meng, X. (2019). MTDH/AEG-1 downregulation using pristimerin-loaded nanoparticles inhibits Fanconi anemia proteins and increases sensitivity to platinum-based chemotherapy. Gynecol Oncol, 155(2), 349-358. doi:10.1016/j.ygyno.2019.08.014

2. Balcerek, J., Jiang, J., Li, Y., Jiang, Q., Holdreith, N., Singh, B., . . . Tong, W. (2018). Lnk/Sh2b3 deficiency restores hematopoietic stem cell function and genome integrity in Fancd2 deficient Fanconi anemia. Nat Commun, 9(1), 3915. doi:10.1038/s41467-018-06380-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FANCD2 Antibody (NB100-316)(16)

Publications using NB100-316

• Pena-Gomez MJ, Moreno-Gordillo P, Narmontė M et al. FANCD2 maintains replication fork stability during misincorporation of the DNA demethylation products 5-hydroxymethyl-2'-deoxycytidine and 5-hydroxymethyl-2'-deoxyuridine Cell death & disease 2022-05-27 [PMID: 35624090] (ICC/IF)
• Sato K, Brandsma I, van Rossum-Fikkert SE et al. HSF2BP negatively regulates homologous recombination in DNA interstrand crosslink repair Nucleic Acids Res. 2020-01-21 [PMID: 31960047] (WB, Human, Mouse)
• Wells JP Proliferating cell nuclear antigen ubiquitination as a central regulator of transcriptional stress Thesis 2023-01-01
• Sabatella M, Pines A, Slyskova J et al. ERCC1-XPF targeting to psoralen-DNA crosslinks depends on XPA and FANCD2 Cell. Mol. Life Sci. 2019-08-07 [PMID: 31392348] (WB, Human)
• Slyskova J, Sabatella M, Ribeiro-Silva C et al. Base and nucleotide excision repair facilitate resolution of platinum drugs-induced transcription blockage Nucleic Acids Res. 2018-08-22 [PMID: 30137419] (ICC/IF, Human)
• Nalepa G, Enzor R, Sun Z et al. Fanconi anemia signaling network regulates the spindle assembly checkpoint. J Clin Invest. 2013-09-03 [PMID: 23934222] (WB, Human)
• Kusayanagi T, Tsukuda S, Shimura S et al. The antitumor agent doxorubicin binds to Fanconi anemia group F protein Bioorg Med Chem 2012-09-16 [PMID: 23026082] (WB, Human)
• Park S, Beck B, Saadatzadeh M et al. Fanconi anemia D2 protein is an apoptotic target mediated by caspases J Cell Biochem 2011-09-01 [PMID: 21520247] (WB, Human)
• Bhagwat N, Olsen AL, Wang AT et al. XPF-ERCC1 participates in the Fanconi anemia pathway of crosslink repair. Mol Cell Biol :MCB.00086-00009. 2009-01-01 [PMID: 19805513]
• Chu PC, Wang TY, Lu YT et al. Involvement of p29 in DNA damage responses and Fanconi anemia pathway. Carcinogenesis 2009-10-01 [PMID: 19748926]
• Si, Y et al. Overnight transduction with foamyviral vectors restores the long-term repopulating activity of Fancc-/- stem cells. Blood;112(12):4458-65. 2008-12-01 [PMID: 18684868]
• Tremblay CS, Huang FF, Habi O, Huard CC, Godin C, Levesque G, Carreau M. HES1 is a novel interactor of the Fanconi anemia core complex. Blood. 112(5):2062-70. Epub 2008 Jun 11. Erratum in: Blood. 2009 Oct 29;114(18):3974. 2008-09-01 [PMID: 18550849]
• Nomura, Y et al. Human Mus81 and FANCB independently contribute to repair of DNA damage during replication. Genes Cells;12(10):1111-22. 2007-10-01 [PMID: 17903171]
• Park, S et al. Oxidative stress/damage induces multimerization and interaction of Fanconi anemia proteins. J Biol Chem;279(29):30053-9. 2004-07-16 [PMID: 15138265] (IP, WB, Human)
• Henrikson K TRANSCRIPTIONAL REGULATION OF SOX5 AND WWOX BY FANCD2 Thesis 2021-01-01 (WB)
• Willers H, Taghian AG, Luo C-M et al. Utility of DNA Repair Protein Foci for the Detection of Putative BRCA1 Pathway Defects in Breast Cancer Biopsies. Mol Cancer Res;7(8):1304-1309. 2009-01-01 [PMID: 19671671]

FAQs for FANCD2 Antibody (NB100-316). (Showing 1 - 2 of 2 FAQs).

1. Can you provide the aa sequence used to generate FANCD2 Antibody (FI-17)? Is it the full length protein? In addition, do you know if this antibody has been shown to recognize isoform 1 and 2 of ID: Q9BXW9?
   • This antibody was generated against the full-length protein. Based on the testing data in our second, cleaner image, this antibody appears to recognize two very close bands in lane 2. These are most likely isoforms 1 and 2.
2. Do you know if this antibody has been shown to recognize isoform 1 and 2 of ID: Q9BXW9?
   • Based on the testing data in our second, cleaner image, this antibody appears to recognize two very close bands in lane 2. These are most likely isoforms 1 and 2.

Bioinformatics

• Gene Symbol: FANCD2
• Entrez:
  • 2177(Human)
  • 211651(Mouse)
• Uniprot:
  • Q9BXW9()