Ferritin Heavy Chain Antibody (FTH/2081) - Azide and BSA Free

Western Blot: Ferritin Heavy Chain Antibody (FTH/2081) - Azide and BSA Free [NBP3-08581] - Western Blot of HeLa, cell lysate using Ferritin Heavy Chain Mouse Monoclonal Antibody (FTH/2081).

Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody (FTH/2081) - Azide and BSA Free [NBP3-08581] - Formalin-fixed, paraffin-embedded human pancreas stained with Ferritin Heavy Chain Mouse Monoclonal Antibody (FTH/2081). Confirmation of Purity and Integrity of Antibody.

Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody (FTH/2081) - Azide and BSA Free [NBP3-08581] - Formalin-fixed, paraffin-embedded human pancreas stained with Ferritin Heavy Chain Mouse Monoclonal Antibody (FTH/2081). Confirmation of Purity and Integrity of Antibody.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: FTH/2081
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Ferritin Heavy Chain Antibody (FTH/2081) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07795).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant human FTH1 protein fragment (around aa 58-180) (exact sequence is proprietary) (exact sequence is proprietary) (Uniprot: P02794)
• Localization: Cytoplasmic
• Marker: Microglia Marker
• Isotype: IgG2c Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FTH1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 0.1-0.2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry Formalin-fixed: 0.1-0.2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Ferritin Heavy Chain Antibody (FTH/2081) - Azide and BSA Free

• apoferritin
• Cell proliferation-inducing gene 15 protein
• Ferritin H subunit
• ferritin heavy chain
• ferritin, heavy polypeptide 1
• FHC
• FTHEC 1.16.3.1
• FTHL6MGC104426
• PIG15
• placenta immunoregulatory factor
• PLIF
• proliferation-inducing protein 15

Background

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FTH1