Fibrillarin Antibody

Western Blot: Fibrillarin Antibody [NBP2-46881] - Western blot analysis of different cell lysates using chicken pAb to fibrillarin NBP2-46881, dilution 1:5,000, in green: [1] protein standard (red), [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, and [6] C6 cells. The single strong band at ~35kDa correspond to the fibrillarin protein.

Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody [NBP2-46881] - Confocal immunofluorescent analysis of HeLa cells stained with chicken pAb to fibrillarin, NBP2-46881, dilution 1:10,000 in green and costained with mouse mAb to vimentin, 1:1,000, in red. The blue signal is DAPI staining of nuclear DNA. The fibrillarin antibody stains nucleoli while the vimentin antibody binds to cytoplasmic intermediate filaments.

• Reactivity: Hu, Mu, Rt, Ca, Eq
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated

Fibrillarin Antibody Summary

• Immunogen: Full length human Fibrillarin expressed in and purified from E.Coli. [UniProt# P22087]
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: FBL
• Purity: IgY purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:2000-1:5000
  • Western Blot 1:2000-1:5000
• Theoretical MW: 34.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY.
• Preservative: 0.035% Sodium Azide
• Purity: IgY purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for Fibrillarin Antibody

• EC 2.1.1,34-kD nucleolar scleroderma antigen
• EC 2.1.1.-
• EC 2.1.1.37
• FIB
• FIB1,34 kDa nucleolar scleroderma antigen
• fibrillarin
• FLRNrRNA 2'-O-methyltransferase fibrillarin
• RNA, U3 small nucleolar interacting protein 1
• RNU3IP1

Background

Fibrillarin / Nop1p, was originally identified as a nucleolar protein of baker's yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is Fibrillarin (accession P22087), a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human Fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the Fibrillarin domain. A Fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologous to the other sequences throughout the entire Fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended beta-sheets flanked by 4 alpha-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be Fibrillarin. Fibrillarin is an excellent marker for the nucleolus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibrillarin Antibody (NBP2-46881)(2)

Publications using NBP2-46881

• Lebdy R, Canut M, Patouillard J et Al. The nucleolar protein GNL3 prevents resection of stalled replication forks EMBO Rep 2023-12-11 [PMID: 37965896]
• Tsaridou S, Velimezi G, Willenbrock F et al. 53BP1-mediated recruitment of RASSF1A to ribosomal DNA breaks promotes local ATM signaling EMBO reports 2022-08-03 [PMID: 35758159] (ICC/IF, Human)
  
  Details:
  1:200 Dilution

FAQs for Fibrillarin Antibody (NBP2-46881). (Showing 1 - 1 of 1 FAQs).

1. What secondary antibody will you suggest for NBP2-46881
   • The isotype of this antibody is IgY; therefore, the secondary should be an anti-IgY antibody.

Bioinformatics

• Gene Symbol: FBL
• Entrez:
  • 2091(Human)
• Uniprot:
  • P22087(Human)