Fibronectin Antibody - BSA Free

Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (//asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY license.

Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (//asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Fibronectin Antibody - BSA Free [NBP1-91258] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-91258 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.

Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Nicotinamide (NAM) attenuates unilateral urethral obstruction (UUO)-induced renal interstitial fibrosis. C57BL/6 mice were subjected to UUO surgery or sham operation. Different doses of NAM or saline were intraperitoneally injected an hour before the surgery and daily thereafter. Representative images of Western blot of FN (fibronectin), alpha-SMA (alpha-smooth muscle actin) and GAPDH (loading control). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30993884/) licensed under a CC-BY license.

Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in HepG2 cell lysate.

Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in NIH 3T3 cell lysate.

Immunocytochemistry/Immunofluorescence: Fibronectin Antibody - BSA Free [NBP1-91258] - Fibronectin antibody was tested in HeLa cells with DyLight 488 (Green). Nuclei and alpha-tubulin were counterstained with DAPI (Blue) and DyLight 550 (Red).

Immunocytochemistry/Immunofluorescence: Fibronectin Antibody - BSA Free [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in human renal cancer using DAB with hematoxylin counterstain.

Immunohistochemistry-Paraffin: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in mouse kidney tissue section using anti-Fibronectin antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in human oesophageal cancer tissue using anti-Fibronectin antibody. Image from verified customer review.

Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Feline corneal stromal cells. IHC-Fr and IF (Alexa Fluor488) were performed using NBP1-91258 at 1:400. Image captured by epifluorescent microscopy. Image from verified customer review.

Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Trabecular meshwork (TM) region of pig eyes. NBP1-91258 Fibronectin antibody was labelled with Alexa Fluor 488 conjugated secondary antibody (Green). DAPI shown as blue. Image from verified customer review.

Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Mouse brain cryosections were stained with Fibronectin and anti-rabbit Alexa Fluor 555. Magnification 20x. Image from vrified customer review.

Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Staining in mouse carotid artery. Image from a verified customer review.

Simple Western: Fibronectin Antibody - BSA Free [NBP1-91258] - Lane view shows a specific band for Fibronectin in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western blot analysis of the effects of IP6, Ins, IP6 + Ins & normal saline on the levels of collagen IV, Lamininand Fibronectin. IP6 or Ins treatment decreased the protein expression of collagen IV, LN & FN, & the combined IP6 + Ins treatment resulted in significantly greater effects compared with treatment with either compound alone. The samples were probed with antibodies against p-collagen IV, p-LN, & p-FN. The Western blot membranes were stripped & reprobed for beta -actin as an internal control to confirm equal loading. (A) representative blots from one of three separate experiments; (B) relative band intensities based on densitometry. The results are expressed as the mean ± standard deviation from three independent experiments. * p < 0.05 compared to the IP6 + Ins group; #p < 0.05 compared to the normal saline group. Image collected & cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/8/5/286), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody - BSA Free [NBP1-91258] - LC depressed both the protein & mRNA level of fibronectin via depletion of peritoneal M2. Values were expressed as the mean ± SD. (A) The overexpression of fibronectin induced by Lactate-4.25% dialysate was evidently downregulated by LC treatment measured by Western blotting; (B) The relative protein level of fibronectin normalized by GAPDH; (C) The mRNA level of fibronectin was depressed by LC treatment; (D) Immunofluorescence staining of fibronectin in the four groups. Blue corresponds to nuclear staining, & red corresponds to fibronectin staining. #p < 0.05 vs. NC & LC group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23685870), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - EMT in cancer cells is regulated by m6A levels of mRNAs. a HeLa & HepG2 cells were treated with or without 10 ng/ml TGF-beta for 3 days, the m6A/A ratio of the total mRNA were determined by LC–MS/MS. b Wound healing of wild-type (control) or Mettl3Mut/− cells was recorded (left) & quantitatively analyzed (right). c Wild-type or Mettl3Mut/− cells were allowed to invade for 24 h & tested by CytoSelect™ 24-well Cell Invasion assay kits (8 µm, colorimetric format); d, e mRNA (d) & protein (e) expressions of MMP2, FN, & E-Cad in wild-type & Mettl3Mut/− HeLa cells were measured by qRT-PCR & western blot analysis, respectively. f HeLa cells were transfected with pcDNA/ALKBH5 or a vector control for 48 h, protein expression was determined by western blot analysis (left) & quantitatively analyzed (right). g Wild-type or Mettl3Mut/− cells were treated with or without 10 ng/ml TGF-beta for 3 days, protein expression was determined by western blot analysis (left) & quantitatively analyzed (right). h The expression of METTL3 in liver cancer & its matched adjacent normal tissues of 50 patients from TCGA database. i Correlation between METTL3 & CDH1 in liver cancer patients (n = 364) from TCGA database. j HeLa cells were pretreated with or without Smad2/3 inhibitor SB431542 (10 μM) & then further treated with 10 ng/ml TGF-beta for 3 days, the m6A/A ratio of the total mRNA were determined by LC–MS/MS. Data are presented as means ± SD from three independent experiments. *p < 0.05, **p < 0.01, NS, no significant, by Student’s t test. Red bar = 200 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31061416), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - EMT in cancer cells is regulated by m6A levels of mRNAs. a HeLa & HepG2 cells were treated with or without 10 ng/ml TGF-beta for 3 days, the m6A/A ratio of the total mRNA were determined by LC–MS/MS. b Wound healing of wild-type (control) or Mettl3Mut/− cells was recorded (left) & quantitatively analyzed (right). c Wild-type or Mettl3Mut/− cells were allowed to invade for 24 h & tested by CytoSelect™ 24-well Cell Invasion assay kits (8 µm, colorimetric format); d, e mRNA (d) & protein (e) expressions of MMP2, FN, & E-Cad in wild-type & Mettl3Mut/− HeLa cells were measured by qRT-PCR & western blot analysis, respectively. f HeLa cells were transfected with pcDNA/ALKBH5 or a vector control for 48 h, protein expression was determined by western blot analysis (left) & quantitatively analyzed (right). g Wild-type or Mettl3Mut/− cells were treated with or without 10 ng/ml TGF-beta for 3 days, protein expression was determined by western blot analysis (left) & quantitatively analyzed (right). h The expression of METTL3 in liver cancer & its matched adjacent normal tissues of 50 patients from TCGA database. i Correlation between METTL3 & CDH1 in liver cancer patients (n = 364) from TCGA database. j HeLa cells were pretreated with or without Smad2/3 inhibitor SB431542 (10 μM) & then further treated with 10 ng/ml TGF-beta for 3 days, the m6A/A ratio of the total mRNA were determined by LC–MS/MS. Data are presented as means ± SD from three independent experiments. *p < 0.05, **p < 0.01, NS, no significant, by Student’s t test. Red bar = 200 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31061416), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Snail is involved in m6A-regulated EMT in cancer cells. a Overlapping of 2.0-fold m6A expression changes in EMT cells & EMT-related functional genes. b m6A peaks were enriched in CDS & 3′UTRs of SNAI1 genes from m6A RIP-seq data. Squares marked increases of m6A peaks in cancer cells undergoing EMT; c m6A RIP-qPCR analysis of SNAI1 mRNA in the control & EMT undergoing HeLa cells. d Protein expression of Snail in Mettl3Mut/− or AKLBH5 transfected (24 h) HeLa cells & the control. e The wound healing of wild-type or Mettl3Mut/− HeLa cells transfected with or without pcDNA/Snail for 48 h were recorded (left) & quantitatively analyzed (right). f Wild-type or Mettl3Mut/− HeLa cells were transfected with or without pcDNA/Snail for 48 h, expression of Snail, FN & E-Cad were measured by western blot analysis. Data are presented as means ± SD from three independent experiments. *p < 0.05, NS, no significant, by Student’s t test. Red bar = 200μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31061416), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - EMT in cancer cells is regulated by m6A levels of mRNAs. a HeLa & HepG2 cells were treated with or without 10 ng/ml TGF-beta for 3 days, the m6A/A ratio of the total mRNA were determined by LC–MS/MS. b Wound healing of wild-type (control) or Mettl3Mut/− cells was recorded (left) & quantitatively analyzed (right). c Wild-type or Mettl3Mut/− cells were allowed to invade for 24 h & tested by CytoSelect™ 24-well Cell Invasion assay kits (8 µm, colorimetric format); d, e mRNA (d) & protein (e) expressions of MMP2, FN, & E-Cad in wild-type & Mettl3Mut/− HeLa cells were measured by qRT-PCR & western blot analysis, respectively. f HeLa cells were transfected with pcDNA/ALKBH5 or a vector control for 48 h, protein expression was determined by western blot analysis (left) & quantitatively analyzed (right). g Wild-type or Mettl3Mut/− cells were treated with or without 10 ng/ml TGF-beta for 3 days, protein expression was determined by western blot analysis (left) & quantitatively analyzed (right). h The expression of METTL3 in liver cancer & its matched adjacent normal tissues of 50 patients from TCGA database. i Correlation between METTL3 & CDH1 in liver cancer patients (n = 364) from TCGA database. j HeLa cells were pretreated with or without Smad2/3 inhibitor SB431542 (10 μM) & then further treated with 10 ng/ml TGF-beta for 3 days, the m6A/A ratio of the total mRNA were determined by LC–MS/MS. Data are presented as means ± SD from three independent experiments. *p < 0.05, **p < 0.01, NS, no significant, by Student’s t test. Red bar = 200 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31061416), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Fibronectin Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made toward the C-terminal region of the human Fibronectin protein (canonical isoform within residues 2300-2380). [Swiss-Prot: P02751]
• Localization: Secreted, extracellular space, extracellular matrix.
• Marker: Mesenchymal Cells Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:400
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Frozen reported by customer review
  • Immunohistochemistry-Paraffin 1:400
  • Simple Western 1:100
  • Western Blot 1:1000
• Application Notes: In Western Blot, a band is seen at ~262 kDa representing Fibronectin. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In IHC-P, staining was observed in the cytoplasm and extracellular space of mouse prostate tissue. Antigen retrieval with 10mM sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100. Separated by Size-Wes, Sally Sue/Peggy Sue. The 12-230kDa separation system and EZ Standard Pack 5 are recommended for detecting human Fibronectin using Simple Western.
• Control:

  HepG2 Whole Cell Lysate

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Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 29439094). Equine reactivity reported in scientific literature (PMID: 30450188). Rat reactivity reported in scientific literature (PMID: 30699330).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Fibronectin Antibody - BSA Free

• CIG
• ED-B
• fibronectin 1
• Fibronectin
• FINC
• FN
• FN1
• FNZ
• GFND
• GFND2
• LETS
• MSF
• SMDCF

Background

Fibronectin (FN-EDA, FN, fibronectin 1) is a glycoprotein that is involved in cell adhesion as well as cell migration processes such as embryogenesis, wound healing, host defense and metastasis. Fibronectin appears to be an important factor in the ability of cancerous cells to metastasize by inducing matrix metalloproteinases (MMPs). In a study of human laryngeal carcinoma cells, researchers showed that FN-induced expression of MMP-9 increased the DNA binding activity of transcription factors NF-kappa-B, Activator protein-1 and Specificity protein-1 to the promoter region (PMID: 20638438). Additionally, a study of renal cell cancer showed that the mRNA expression of fibronectin 1 was higher in patients with renal cell cancer as compared to patients with healthy kidneys. This disparity in mRNA expression levels could potentially be used to determine the aggressiveness of renal cell cancer as well as indicate the progression of the disease in patients (PMID: 20860816). Furthermore, a knockdown study of endogenous FN in the A431-III cell line showed a significant decrease of the migratory and invasive activity of the cells as well as an inhibition of secreted MMP-9 (PMID: 21036738).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibronectin Antibody (NBP1-91258)(56)

Publications using NBP1-91258

• Nan Zhang, Pengyu Zhang, Xizhi Deng, Min Zhu, Yixin Hu, Dongxiao Ji, Lufan Li, Yang Liu, Wen Zeng, Min Ke Protective Effect of Nicotinamide Riboside on Glucocorticoid-Induced Glaucoma: Mitigating Mitochondrial Damage and Extracellular Matrix Deposition Investigative Ophthalmology & Visual Science 2024-07-01 [PMID: 38949632]
• Ana C. Acosta, Hadi Joud, Mei Sun, Marcel Y. Avila, Curtis E. Margo, Edgar M. Espana Keratocyte-Derived Myofibroblasts: Functional Differences With Their Fibroblast Precursors Investigative Ophthalmology & Visual Science 2023-10-05 [PMID: 37796488]
• Sung MS, Kim SY, Eom GH, Park SW High VEGF Concentrations Accelerate Human Trabecular Meshwork Fibrosis in a TAZ-Dependent Manner International journal of molecular sciences 2023-06-01 [PMID: 37298577] (WB, Human)
• Barreto R, Carvalho H, Matias G et al. THE EXTRACELLULAR MATRIX PROTEIN PATTERN IN THE CANINE NEOPLASTIC MAMMARY GLAND Tissue and Cell 2023-02-01 [PMID: 36933273] (IHC, Canine)
• Wu X, Zhang D, Qiao X et al. Regulating the cell shift of endothelial cell-like myofibroblasts in pulmonary fibrosis The European respiratory journal 2023-06-01 [PMID: 36758986] (Western Blot, Mouse)
• Li XF, Selli C, Zhou HL et al. Macrophages promote anti-androgen resistance in prostate cancer bone disease The Journal of experimental medicine 2023-04-03 [PMID: 36749798] (IHC-P, ICC/IF, Mouse)
  
  Details:
  Dilution used in IHC-P and ICC 1:500
• Jung S, Jiang L, Zhao J et al. Clathrin Light Chain Conjugated Drug Delivery for Cancer Bioengineering & Translational Medicine 2021-11-28 [PMID: 36684105] (IHC-Fr, Mouse)
• Kuczwara V, Schuler G, Pfarrer C et al. Ultrastructural and Immunohistochemical Characterization of Maternal Myofibroblasts in the Bovine Placenta around Parturition Veterinary Sciences 2023-01-07 [PMID: 36669044] (Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Mouse)
• Rüegg AB, Kowalewski MP, Ulbrich SE Endometrial extracellular matrix components do not change over the course of embryonic diapause and reactivation in the roe deer (Capreolus capreolus) Reproduction in domestic animals = Zuchthygiene 2023-05-01 [PMID: 36645739] (Immunohistochemistry)
• Wu M, Matar DY, Yu Z et al. Continuous NPWT Regulates Fibrosis in Murine Diabetic Wound Healing Pharmaceutics 2022-10-06 [PMID: 36297560] (IHC-P, Mouse)
• Gu L, Li A, Lin J et al. Knockdown of SOX9 alleviates tracheal fibrosis through the Wnt/B-catenin signaling pathway Journal of molecular medicine (Berlin, Germany) 2022-11-01 [PMID: 36192639]
• Lee GH, Cheon J, Kim D, Jun HS. Lysophosphatidic Acid Promotes Epithelial-Mesenchymal Transition in Kidney Epithelial Cells via the LPAR1/MAPK-AKT/KLF5 Signaling Pathway in Diabetic Nephropathy International Journal of Molecular Sciences 2022-09-10 [PMID: 36142408] (Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Mouse)
• Passanha FR, Geuens T, LaPointe VLS Cadherin-11 Influences Differentiation in Human Mesenchymal Stem Cells by Regulating the Extracellular Matrix Via the TGF?1 Pathway Stem cells (Dayton, Ohio) 2022-07-27 [PMID: 35416252] (WB, ICC/IF, Human)
• Sterken BA, Ackermann T, MUller C et al. C/EBP beta isoform-specific regulation of migration and invasion in triple-negative breast cancer cells NPJ breast cancer 2022-01-18 [PMID: 35042889] (WB, Human)
• Zhang D, Liu B, Jie X et al. Uncovering Bupi Yishen Formula Pharmacological Mechanisms Against Chronic Kidney Disease by Network Pharmacology and Experimental Validation Frontiers in Pharmacology 2021-11-15 [PMID: 34867380] (Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Mouse)
• Carrick FR, Valerio LSA, Gonzalez-Vega MN, Engel D Accelerated Wound Healing Using a Novel Far-Infrared Ceramic Blanket Life 2021-01-01 [PMID: 34575027] (WB, Mouse)
• Wen S, Deng F, Li L et al. VX-765 ameliorates renal injury and fibrosis in diabetes by regulating caspase-1-mediated pyroptosis and inflammation Journal of diabetes investigation 2021-09-08 [PMID: 34494385] (IHC-P, WB, Mouse, Human)
• S Dey, LM Udari, P RiveraHern, JJ Kwon, B Willis, JJ Easler, EL Fogel, S Pandol, J Kota Loss of miR-29a/b1 promotes inflammation and fibrosis in acute pancreatitis JCI Insight, 2021-10-08;0(0):. 2021-10-08 [PMID: 34464354] (Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Mouse)
• Geuens T, Ruiter FAA, Schumacher A et al. Thiol-ene cross-linked alginate hydrogel encapsulation modulates the extracellular matrix of kidney organoids by reducing abnormal type 1a1 collagen deposition Biomaterials 2021-06-19 [PMID: 34198162] (IF/IHC, Human)
• Hupy ML, Pedler MG, Shieh B et al. Suppression of epithelial to mesenchymal transition markers in mouse lens by a Smad7-based recombinant protein Chemico-Biological Interactions 2021-08-01 [PMID: 33961834] (Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Mouse)
• Chen X, Li C, Liu J et al. Inhibition of ER stress by targeting the IRE1alpha-TXNDC5 pathway alleviates crystalline silica-induced pulmonary fibrosis International immunopharmacology 2021-03-07 [PMID: 33691254] (IHC-P, Mouse)
• Jiang L, Jung S, Zhao J et al. Simultaneous targeting of primary tumor, draining lymph node, and distant metastases through high endothelial venule-targeted delivery Nano Today 2021-02-01 [PMID: 33391389] (ICC/IF, Mouse)
  
  Details:
  Immunofluorescence was performed on mouse cancer cells.
• Liu Y, Zhang H, Wang S et al. Reduced pericyte and tight junction coverage in old diabetic rats are associated with hyperglycemia-induced cerebrovascular pericyte dysfunction American journal of physiology. Heart and circulatory physiology 2020-12-11 [PMID: 33306445]
• Jiang L, Wang YJ, Zhao J et al. Direct Tumor Killing and Immunotherapy through Anti-SerpinB9 Therapy Cell 2020-11-25 [PMID: 33242418] (Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Mouse)
• Roberts AL, Mavlyutov TA, Perlmutter TE et al. Fibronectin extra domain A (FN-EDA) elevates intraocular pressure through Toll-like receptor 4 signaling Sci Rep 2020-06-17 [PMID: 32555351] (IHC-P, Mouse)
• Schmitt C, Lechanteur A, Cossais F et al. Liposomal Encapsulated Curcumin Effectively Attenuates Neuroinflammatory and Reactive Astrogliosis Reactions in Glia Cells and Organotypic Brain Slices International Journal of Nanomedicine 2020-05-25 [PMID: 32547020] (Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Mouse)
• Sen P, Helmke A, Liao CM et al. SerpinB2 Regulates Immune Response in Kidney Injury and Aging Journal of the American Society of Nephrology 2020-05-01 [PMID: 32209589] (Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Mouse)
• Wen S, Li S, Li L, Fan Q circACTR2: A Novel Mechanism Regulating High Glucose-Induced Fibrosis in Renal Tubular Cells via Pyroptosis Biol. Pharm. Bull. 2020-03-03 [PMID: 32115515] (WB, Human)
• Wen S, Wang ZH, Zhang CX et al. Caspase-3 Promotes Diabetic Kidney Disease Through Gasdermin E-Mediated Progression to Secondary Necrosis During Apoptosis Diabetes Metab Syndr Obes 2020-02-10 [PMID: 32104028] (WB, Human, Mouse)
• Sharma S, Plotkin M Id1 expression in kidney endothelial cells protects against diabetes-induced microvascular injury FEBS Open Bio. [PMID: 31957231] (IF/IHC, ICC/IF, Mouse)
• He X, Chen S, Li C et al. Trehalose Alleviates Crystalline Silica-Induced Pulmonary Fibrosis via Activation of the TFEB-Mediated Autophagy-Lysosomal System in Alveolar Macrophages Cells 2020-01-04 [PMID: 31947943] (IF/IHC, Mouse)
• Hernandez H, Roberts AL, McDowell CM Nuclear factor-kappa beta signaling is required for transforming growth factor Beta-2 induced ocular hypertension Exp. Eye Res. 2020-01-08 [PMID: 31923415] (IHC-P, Mouse)
• Fu C, Lu Y, Williams MA, et al. Emergency myelopoiesis contributes to immune cell exhaustion and pulmonary vascular remodeling Br. J. Pharmacol. 2019-12-03 [PMID: 31793661] (WB, Mouse)
• Landry NM, Rattan SG, Dixon IMC An Improved Method of Maintaining Primary Murine Cardiac Fibroblasts in Two-Dimensional Cell Culture Sci Rep 2019-09-09 [PMID: 31501457] (WB, Mouse)
• Barreto RS, Romagnolli P, Fratini P et al. Mouse placental scaffolds: a three-dimensional environment model for recellularization J Tissue Eng 2019-08-08 [PMID: 31448074] (IHC-P, Mouse)
• Xu L, Sharkey D, Cantley LG Tubular GM-CSF Promotes Late MCP-1/CCR2-Mediated Fibrosis and Inflammation after Ischemia/Reperfusion Injury J. Am. Soc. Nephrol. 2019-07-17 [PMID: 31315923] (WB, Mouse)
• Borghesi J, da Silva Ferreira H, Favaron PO et al. Characterization of the extracellular matrix in the chorioallantoic membrane of water buffalo (Bubalus bubalis) in early gestation Reprod. Domest. Anim. 2019-07-06 [PMID: 31278783] (IF/IHC)
• Wang P, Wang WJ, Choi-Nurvitadhi J et al. RNA m6A methylation regulates the epithelial mesenchymal transition of cancer cells and translation of Snail Nat Commun 2019-05-06 [PMID: 31061416] (WB, Human)
• Zheng, M;Cai, J;Liu, Z;Shu, S;Wang, Y;Tang, C;Dong, Z; Nicotinamide reduces renal interstitial fibrosis by suppressing tubular injury and inflammation J. Cell. Mol. Med. 2019-04-16 [PMID: 30993884] (WB, Mouse)
• Zha D, Yao T, Bao L et al. Telmisartan attenuates diabetic nephropathy progression by inhibiting the dimerization of angiotensin type-1 receptor and adiponectin receptor-1. Life Sci. 2019-01-27 [PMID: 30699330] (WB, Rat)
• da Palma RK, Fratini P, Schiavo Matias GS et al. Equine lung decellularization: a potential approach for in vitro modeling the role of the extracellular matrix in asthma. J Tissue Eng. 2018-11-12 [PMID: 30450188] (IHC-P, Equine)
• Matias GSS, Rigoglio NN, Carreira ACO et al. Optimization of Canine Placenta Decellularization: An Alternative Source of Biological Scaffolds for Regenerative Medicine. Cells Tissues Organs (Print) 2018-09-10 [PMID: 30199873] (IHC-P, Canine)
• Bao H, Sin TK, Zhang G. Activin A induces leiomyoma cell proliferation, extracellular matrix (ECM) accumulation and myofibroblastic transformation of myometrial cells via p38 MAPK. Biochem. Biophys. Res. Commun. 2018-10-02 [PMID: 30195496] (WB, ICC/IF, Human)
• Eissa LA, Kenawy HI, El-Karef A et al. Antioxidant and anti-inflammatory activities of berberine attenuate hepatic fibrosis induced by thioacetamide injection in rats Chem. Biol. Interact. 2018-08-21 [PMID: 30138605] (IHC-P, Rat)
• Jung MY, Kang JH, Hernandez DM, Yin X. Fatty acid synthase is required for profibrotic TGF-b signaling. FASEB J. 2018-02-16 [PMID: 29475397] (IF/IHC, Mouse)
• Graubner FR, Boos A, Aslan S, Kucukaslan I. Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog. Reproduction. 2018-02-09 [PMID: 29439094] (Canine)
• da Silva Nunes Barreto R, Romagnolli P, Mess AM, Miglino MA. Decellularized bovine cotyledons may serve as biological scaffolds with preserved vascular arrangement J Tissue Eng Regen Med. 2017-11-21 [PMID: 29164819] (Bovine)
• Chiang KC, Yeh CN, Pang JS et al. 1a,25(OH)2D3 Analog, MART-10, Inhibits Neuroendocrine Tumor Cell Metastasis After VEGF-A Stimulation. Anticancer Res. 2017-11-01 [PMID: 29061804] (Human)
• El-Mihi KA, Kenawy HI, El-Karef A et al. Naringin attenuates thioacetamide-induced liver fibrosis in rats through modulation of the PI3K/Akt pathway Life Sci. 2017-08-19 [PMID: 28830755] (IF/IHC, Rat)
• Lin Yu-Jung, Shyu Woei-Cherng, Chang Chi-Wei et al. Tumor Hypoxia Regulates Forkhead Box C1 to Promote Lung Cancer Progression. Theranostics 2019-03-05 [PMID: 28435461] (WB, Human)
• Fu M, Song Y, Wen Z et al. Inositol Hexaphosphate and Inositol Inhibit Colorectal Cancer Metastasis to the Liver in BALB/c Mice. Nutrients [PMID: 27187454]
• Nitta Kyoko, Shi Sen, Nagai Takako et al. Oral AdministRation of N-Acetyl-seryl-aspartyl-lysyl-proline AmelioRates Kidney Disease in Both Type 1 and Type 2 Diabetic Mice via a Therapeutic Regimen. BioMed Research International 2016-01-01 [PMID: 27088094] (WB, Mouse)
• Green CJ, Fraser ST, Day ML. Insulin-like growth factor 1 increases apical fibronectin in blastocysts to increase blastocyst attachment to endometrial epithelial cells in vitro. Hum. Reprod. 2015-02-01 [PMID: 25432925] (ICC/IF, Mouse)
  
  Details:
  Fibronectin/Anastellin antibody used for ICC-IF on Day 5 blastocysts that were cultured in serum starved medium for 24 , 48 or 72 h in the absence or presence of IGF1 or in the presence of a PI3K inhibitor LY294002 or in the presence of IGF1, attached to a coverslip - 15 minute 4% paraformaldehyde fixation, 30 minutes permeablization with PBS-Polyvinyl alcohol-0.3% Triton X100, blocking in PPTB (PBS + PVA + 0.1% Tween-20 + 0.7% BSA), ON 4C incubation of primary, anti-rabbit Alexa 488 secondary antibody (Figure 5).
• Medina-Ortiz WE, Belmares R, Neubauer S et al. Cellular Fibronectin Expression in Human Trabecular Meshwork and Induction by Transforming Growth Factor-beta2. Invest Ophthalmol Vis Sci. 2013-10-17 [PMID: 24030464]
• Hosick PA, Alamodi AA, Storm MV et al. Chronic carbon monoxide treatment attenuates development of obesity and remodels adipocytes in mice fed a high-fat diet. Int J Obes (Lond) 2013-04-30 [PMID: 23689359] (WB, Mouse)
• Wang J, Jiang ZP, Su N et al. The role of peritoneal alternatively activated macrophages in the process of peritoneal fibrosis related to peritoneal dialysi. Int J Mol Sci 2013-05-17 [PMID: 23685870] (IHC-P, ICC/IF, Mouse)

Reviews for Fibronectin Antibody (NBP1-91258) (11)

Average Rating: 4.2

(Based on 11 reviews)

We have 11 reviews tested in 4 species: Human, Mouse, Feline, Pig.

reviewed by: Verified Customer WB Mouse 10/02/2020

Summary

• Application: Western Blot
• Sample Tested: Mouse Pancreas
• Species: Mouse
• Lot: N/A

reviewed by: Verified Customer WB Mouse 10/02/2020

Summary

• Application: Western Blot
• Sample Tested: Pancreas
• Species: Mouse
• Lot: Lot B2

reviewed by: Verified Customer WB Human 06/17/2019

Summary

• Application: Western Blot
• Sample Tested: SVG-A whole cell lysate
• Species: Human
• Lot: D3

reviewed by: Kirsten Hattermann-Koch IHC-Fr Mouse 05/09/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse brain
• Species: Mouse
• Lot: D3

reviewed by: Verified Customer IHC-Fr Pig 12/16/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: trabecular meshwork (TM) region of pig eyes
• Species: Pig
• Lot: D-2

reviewed by: Verified Customer IHC-Fr Feline 04/27/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: corneal stromal cells
• Species: Feline
• Lot: D-1

reviewed by: Verified Customer IF Mouse 07/22/2016

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse Kidneys with Alport Disease
• Species: Mouse
• Lot: A-2

reviewed by: Verified Customer WB Mouse 04/07/2016

Summary

• Application: Western Blot
• Sample Tested: Mouse Atherosclerotic Plaque
• Species: Mouse
• Lot: A-4

reviewed by: Lindsey Davis ICC Mouse 06/06/2015

Summary

• Application: Immunocytochemistry
• Species: Mouse

reviewed by: Verified Customer ICC Human 04/30/2015

Summary

• Application: Immunocytochemistry
• Species: Human

reviewed by: Verified Customer IHC-P Mouse 03/02/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse kidney
• Species: Mouse
• Lot: A-2

Bioinformatics

• Gene Symbol: FN1
• Entrez:
  • 2335(Human)
• Uniprot:
  • P02751()