FoxM1 Antibody - BSA Free

Western Blot: FoxM1 Antibody [NBP1-30961] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody [C3], C-term diluted at 1:5000.

Immunohistochemistry-Paraffin: FoxM1 Antibody [NBP1-30961] - Representative images of immunoreactivity of nuclear FOXM1 in (I) benign cystadenoma, (II) borderline tumour, (III) stage I invasive cancer, (IV) stage II invasive cancer, (V) stage III invasive cancer and (VI) stage IV invasive cancer. Magnifications X400. Insets: Regions with higher magnifications of nuclear FOXM1 staining. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0113478), licensed under a CC-BY license.

• Reactivity: Hu, Rt
• Applications: WB, IHC, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

FoxM1 Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FoxM1. The exact sequence is proprietary.
• Localization: Nucleus
• Marker: Mitosis Marker
• Predicted Species: Rat (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FOXM1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Application Notes: IHC-P reactivity reported in (PMID: 25411964). ChIP assay- Assay dependent.
• Theoretical MW: 84 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (83%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for FoxM1 Antibody - BSA Free

• FKHL16
• FKHL16trident
• forkhead box M1
• forkhead box protein M1
• Forkhead, drosophila, homolog-like 16
• forkhead-like 16
• Forkhead-related protein FKHL16
• FoxM1
• Hepatocyte nuclear factor 3 forkhead homolog 11
• HFH-11
• HFH11FOXM1B
• HFH-11M-phase phosphoprotein 2
• HNF-3
• HNF-3/fork-head homolog 11
• INS-1
• MPHOSPH2
• MPP-2
• MPP2MPM-2 reactive phosphoprotein 2
• PIG29
• TGT3
• Transcription factor Trident
• Trident
• WIN
• Winged-helix factor from INS-1 cells

Background

FOXM1 belongs to a large family of Forkhead transcription factors that possesses a winged-helix DNA-binding domain called Forkhead box (FOX). It is a transcription factor critical to cell cycle progression via its role in coordinating the G2/M phase transition. FOXM1 is ubiquitously expressed in proliferating cells but is absent in differentiated cells. FOXM1 overexpression has been detected in a variety of tumor types including breast, lung, prostate, and liver cancer, and appears to be involved in tumorigenesis-associated processes such as angiogenesis and metastasis. In light of these findings, FOXM1 has recently become an attractive target for anti-cancer therapy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FoxM1 Antibody (NBP1-30961)(4)

Publications using NBP1-30961

• Alvandi Z, Nagata Y, Passos LSA et al. Wnt Site Signaling Inhibitor Secreted Frizzled-Related Protein 3 Protects Mitral Valve Endothelium From Myocardial Infarction-Induced Endothelial-to-Mesenchymal Transition Journal of the American Heart Association 2022-04-05 [PMID: 35348006] (Immunohistochemistry-Paraffin, Human)
  
  Details:
  1:300 IHC-Fr dilution
• Misra R, Patra B, Varadaraj S, Verma RS Establishing the promising role of novel combination of triple therapeutics delivery using polymeric nanoparticles for Triple negative breast cancer therapy Bioimpacts 2021-08-02 [PMID: 34336608]
• Silva LS, Goncalves LG, Silva F et al. STAT3:FOXM1 and MCT1 drive uterine cervix carcinoma fitness to a lactate-rich microenvironment. Tumour Biol. 2015-11-12 [PMID: 26563366]
• Zhao F, Siu Mk, Jiang L et al. Overexpression of Forkhead Box Protein M1 (FOXM1) in Ovarian Cancer Correlates with Poor Patient Survival and Contributes to Paclitaxel Resistance. PLoS OnE. 2014-11-21 [PMID: 25411964] (IHC-P, Human)

Review for FoxM1 Antibody (NBP1-30961) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/08/2019

Summary

• Application: Western Blot
• Sample Tested: 3 human pancreas cancer cell lines
• Species: Human
• Lot: 42613

Bioinformatics

• Gene Symbol: FOXM1
• Entrez:
  • 2305(Human)
• Uniprot:
  • Q08050()