gamma Tubulin Antibody

Western Blot: gamma Tubulin Antibody [NB120-11318] - Cell lysates (lanes 1-7) were separated on SDS-PAGE, blotted with 0.7 ug/mL Anti-gamma-Tubulin, and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. U87; 2. HeLa; 3. Jurkat; 4. C2C12; 5. Rat2; 6. MDCK; 7. A431

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in Rat2 cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in NIH3T3 cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in NIH3T3 cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in HeLa cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.

• Reactivity: Hu, Ca, Ch
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.7 mg/ml

gamma Tubulin Antibody Summary

• Immunogen: Synthetic peptide: EEFATEGTDRKDVFFY-K, corresponding to amino acids 38-53 of Human gamma Tubulin.
• Localization: Centrosomal
• Marker: Centrosome Marker
• Specificity: Does not react with alpha, beta, omega and eta Tubulin. The IF testing of this product is performed using chicken fibroblasts. We have not performed IF testing using human cells. This antibody is tested in western blotting using the following cell lysates ; U87, HeLa, Jurkat, C2C12, Rat2, MDCK, and A431.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TUBG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Western Blot 1:1000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

The antigen sequence is identical in mouse and rat Gamma-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast Gamma-tubulin).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and 1.0% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.7 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for gamma Tubulin Antibody

• gamma Tubulin
• Gamma-1-tubulin
• Gamma-tubulin complex component 1
• GCP-1
• TUBG
• TUBG1
• TUBGCP1
• tubulin gamma-1 chain
• tubulin, gamma 1
• tubulin, gamma polypeptide

Background

The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The g-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing g-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for gamma Tubulin Antibody (NB120-11318)(1)

Publication using NB120-11318

• Heckler MM, Zeleke TZ, Divekar SD et al. Antimitotic activity of DY131 and the estrogen-related receptor beta 2 (ERRb2) splice variant in breast cancer Oncotarget 2016-07-26 [PMID: 27363015] (ICC/IF, Human)

FAQs for gamma Tubulin Antibody (NB120-11318). (Showing 1 - 1 of 1 FAQ).

1. Does this antibody require methanol fixation for immunofluorescence use?
   • This antibody was tested using methanol/acetone-fixed chicken fibroblast cells but that does not necessarily mean that methanol fixation is required. This antibody should work using whatever protocol you prefer.

Bioinformatics

• Gene Symbol: TUBG1