GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-320] - Staining of Human Liver (A) and Tonsil (B) with antibody at 0.001 ug/mL and Rat Brain lysate (C) with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence. Approx. 37 kDa band observed in Rat Brain lysates and approx. 35 kDa in Human Liver and Tonsil lysates (calculated MW of 36.1 kDa according to Human NP_002037.2 and 35.8 kDa according to Rat NP_058704.1).

Western Blot: GAPDH Antibody [NB300-320] - Staining of Human Liver (A) and Tonsil (B) with antibody at 0.001 ug/mL and Rat Brain lysate (C) with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence. Approx. 37 kDa band observed in Rat Brain lysates and approx. 35 kDa in Human Liver and Tonsil lysates (calculated MW of 36.1 kDa according to Human NP_002037.2 and 35.8 kDa according to Rat NP_058704.1).

Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-320] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL). Strong expression of the protein seen in the cytoplasm and vesicles of HeLa cells.

Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-320] - Staining of paraffin embedded Human Pancreas. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: GAPDH Antibody [NB300-320] - stai Mutants Have Reduced Levels of alpha-Tubulin, Acetylated alpha-Tubulin, and Kinesin Heavy Chain Protein. Representative western blots of total protein extracted from stai mutant third instar larvae. Heterozygous stai/+ mutant larvae exhibit mild reductions in the levels of alpha-tubulin and acetylated alpha-tubulin compared with wild type larvae. Homozygous stai mutant larvae have dramatic reductions in the levels of alpha-tubulin, acetylated alpha-tubulin and the heavy chain subunit of the microtubule motor protein kinesin compared to wildtype. Df(2L) BSC239 represents a second chromosomal deficiency that excludes the stai gene. GAPDH is used as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0068324), licensed under a CC-BY license.

Western Blot: GAPDH Antibody [NB300-320] - Staining of HEK293 (A) and HeLa (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.001 ug/mL. Detected by chemiluminescence. Approx 36 kDa band observed in lysates of cell line HEK293 and approx. 35 kDa in HeLa cell lysates.(calculated MW of 36.1 kDa according to Human NP_002037.2).

Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-320] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL). Strong expression of the protein seen in the cytoplasm and vesicles of A549 cells.

Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-320] - Upregulation of GAPDH and Tom20 in C6 gliomas. A small glioma labeled with antibodies against GAPDH. The brightness and contrast have been increased. Image collected and cropped by CiteAb from the following publication (//www.biomedcentral.com/1756-0500/8/207), licensed under a CC-BY license.

Upregulation of GAPDH & Tom20 in C6 gliomas. a A section of rat brain stained with hematoxilin & eosin to show a glioma produced by implantation of C6 cells in the right caudate nucleus. In this case, cells also grew in the region of the syringe needle track through the cortex. (b–d). A small glioma labeled by bisbenzamide (b) & with antibodies against Tom20 (c) & GAPDH (d). The brightness & contrast have been increased. e Illustration of a strip perpendicular to the rim of a glioma (labeled with bisbenzamide) & along which intensity profiles were measured. f, g Tom20 & GAPDH fluorescence along such a strip (barely visible without enhancement). The graphs show the raw intensity values given by ImageJ. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26032618), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody [NB300-320] - PAR2 causes contraction of smooth muscle cells from human prostate. (A & B) Decrease in diameter of collagen hydrogels after PAR2 activation with SLIGKV (80 µM). (C) Representative western blot & (D) densitometry showing time dependent increase in level of phosphorylated MLC20 in PSMC after PAR2 is activated. Data represent mean ± SEM of at least three independent experiments. Diameter of collagen hydrogels were measured in ImageJ (version 1.50i) & significance analyzed in Prism (version 7.04) with one‐way ANOVA followed by Tukey's multiple comparison test. *P < 0.05, **P < 0.01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31198907), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: GAPDH Antibody [NB300-320] - Upregulation of GAPDH & Tom20 in C6 gliomas. a A section of rat brain stained with hematoxilin & eosin to show a glioma produced by implantation of C6 cells in the right caudate nucleus. In this case, cells also grew in the region of the syringe needle track through the cortex. (b–d). A small glioma labeled by bisbenzamide (b) & with antibodies against Tom20 (c) & GAPDH (d). The brightness & contrast have been increased. e Illustration of a strip perpendicular to the rim of a glioma (labeled with bisbenzamide) & along which intensity profiles were measured. f, g Tom20 & GAPDH fluorescence along such a strip (barely visible without enhancement). The graphs show the raw intensity values given by ImageJ. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26032618), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody [NB300-320] - CDC20B was the protein effector regulated by hsa-miR-633. (a) CDC20B level was up-regulated after ox-LDL treatment revealed by Western blotting. (b) CDC20B level was decreased after the treatment of hsa-miR-633 mimics revealed by Western blotting. (c) CDC20B level was increased after the treatment of hsa-miR-633 inhibitor revealed by Western blotting. (d) The luciferase reporter plasmids were constructed as illustrated. (e) Relative luciferase activities after co-transfection CDC20B WT & hsa-miR-633/Control, or CDC20B Mut & hsa-miR-633/Control were measured. (f) The expression levels of two proliferation markers, Ki67 & PCNA after the treatment of si-CDC20B were measured using Western blotting. (g) Colony formation assay was conducted to examine the proliferation abilityafter the treatment of si-CDC20B. (h) Transwell migration & invasion assays were conducted to examine the migration & invasion abilities of VSMCs after the treatment of si-CDC20B. (i) Quantification results of (g). (j) Quantification results of (h). *P < 0.05, **P < 0.01, ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35212610), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Simple Western: GAPDH Antibody [NB300-320] - MiR-34a affects stroke outcomes via interacting with cytochrome c. (A) WES system image showing CYC, VDAC & GAPDH expression from hemispheres of WT & miR-34a−/− mice at 6 h post-stroke. (B) Relative CYC expression normalized to GAPDH from the data generated by the WES system. CYC level is significantly decreased in ischemic hemispheres of WT mice but no significant changes in miR-34a−/− mice between contralateral hemispheres (Control) & ischemic hemispheres (Ischemia). N = 5 per group, **p < 0.01, One-way ANOVA followed by post hoc Tuckey’s test was used for data analysis. Data are expressed as mean ± S.D. (C) Relative VDAC expression normalized to GAPDH from the data generated by the WES system. VDAC was not significantly altered in WT mice nor miR-34a−/− mice. (D) Multiplexed WES system image showing CYC & GAPDH expression from purified pCECs of WT & miR-34a−/− mice (n = 10 per group, pooled cell samples) at 6 h post-stroke. (E) Relative CYC expression by normalization to GAPDH. A 2.8 fold decrease of CYC level was observed in WT mice but no changes were observed in miR-34a−/− mice between contralateral hemispheres & ischemic hemispheres. (F) A CYC reporter was coexpressed with a miR-34a plasmid, a miR-34a mimic, a miR-34c mimic, or a plasmid control in cultured cerebral vascular endothelial cells for 24 hours. Relative firefly luciferase activity was evaluated & normalized to renilla luciferase activity. Relative firefly luciferase activity was reduced by miR-34a plasmid & miR-34a mimic. The experiment was repeated 3 times & triplicates were used for each analysis. Data represents the mean ± S.D. *p < 0.05. One-way ANOVA followed by post-hoc Tuckey’s test was used for analysis. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32094435), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody [NB300-320] - Hsa-miR-633 inhibitor could reverse the si-circ_0008896 phenotypes. (a) CCK8 assay was conducted to measure cell viability after up-regulation of hsa-miR-633. (b) The expression levels of two proliferation markers, Ki67 & PCNA after up-regulation of hsa-miR-633 were measured using Western blotting. (c) CCK8 assay was conducted to measure cell viability after the treatment of si-circ0008896, hsa-miR-633 inhibitor & si-circ0008896+ hsa-miR-633 inhibitor. (d) The expression levels of two proliferation markers, Ki67 & PCNA after the treatment of si-circ0008896, hsa-miR-633 inhibitor & si-circ0008896+ hsa-miR-633 inhibitor were measured using Western blotting. (e) Colony formation assay was conducted to examine proliferation ability number of colonies after the treatment of si-circ0008896, hsa-miR-633 inhibitor & si-circ0008896+ hsa-miR-633 inhibitor. (f) Quantification results of (e). (g) Transwell migration & invasion assays were conducted to examine the migration & invasion abilities of VSMCs after the treatment of si-circ0008896, hsa-miR-633 inhibitor & si-circ0008896+ hsa-miR-633 inhibitor. (h) Quantification results of (g). *P < 0.05, **P < 0.01, ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35212610), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody [NB300-320] - Down-regulation of hsa_circ_0008896 inhibited proliferation, migration & invasion in vitro. (a) The expression level of hsa_circ_0008896 after si-circ_0008896 transfection was detected using quantitative PCR. (b) CCK8 assay was conducted to measure cell viability after down-regulation of hsa_circ_0008896. (c) The expression levels of two proliferation markers, Ki67 & PCNA after down-regulation of hsa_circ_0008896 were detected using Western blotting. (d) Colony formation assay was conducted to examine proliferation ability after down-regulation of hsa_circ_0008896. (e) The quantification results of (d). (f) Transwell migration & invasion assays were conducted to examine the migration & invasion abilities of VSMCs after down-regulation of hsa_circ_0008896. (g) The quantification results of (f). *P < 0.05, **P < 0.01, ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35212610), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody [NB300-320] - CDC20B was the protein effector regulated by hsa-miR-633. (a) CDC20B level was up-regulated after ox-LDL treatment revealed by Western blotting. (b) CDC20B level was decreased after the treatment of hsa-miR-633 mimics revealed by Western blotting. (c) CDC20B level was increased after the treatment of hsa-miR-633 inhibitor revealed by Western blotting. (d) The luciferase reporter plasmids were constructed as illustrated. (e) Relative luciferase activities after co-transfection CDC20B WT & hsa-miR-633/Control, or CDC20B Mut & hsa-miR-633/Control were measured. (f) The expression levels of two proliferation markers, Ki67 & PCNA after the treatment of si-CDC20B were measured using Western blotting. (g) Colony formation assay was conducted to examine the proliferation abilityafter the treatment of si-CDC20B. (h) Transwell migration & invasion assays were conducted to examine the migration & invasion abilities of VSMCs after the treatment of si-CDC20B. (i) Quantification results of (g). (j) Quantification results of (h). *P < 0.05, **P < 0.01, ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35212610), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Simple Western: GAPDH Antibody [NB300-320] - MiR-34a affects stroke outcomes via interacting with cytochrome c. (A) WES system image showing CYC, VDAC & GAPDH expression from hemispheres of WT & miR-34a−/− mice at 6 h post-stroke. (B) Relative CYC expression normalized to GAPDH from the data generated by the WES system. CYC level is significantly decreased in ischemic hemispheres of WT mice but no significant changes in miR-34a−/− mice between contralateral hemispheres (Control) & ischemic hemispheres (Ischemia). N = 5 per group, **p < 0.01, One-way ANOVA followed by post hoc Tuckey’s test was used for data analysis. Data are expressed as mean ± S.D. (C) Relative VDAC expression normalized to GAPDH from the data generated by the WES system. VDAC was not significantly altered in WT mice nor miR-34a−/− mice. (D) Multiplexed WES system image showing CYC & GAPDH expression from purified pCECs of WT & miR-34a−/− mice (n = 10 per group, pooled cell samples) at 6 h post-stroke. (E) Relative CYC expression by normalization to GAPDH. A 2.8 fold decrease of CYC level was observed in WT mice but no changes were observed in miR-34a−/− mice between contralateral hemispheres & ischemic hemispheres. (F) A CYC reporter was coexpressed with a miR-34a plasmid, a miR-34a mimic, a miR-34c mimic, or a plasmid control in cultured cerebral vascular endothelial cells for 24 hours. Relative firefly luciferase activity was evaluated & normalized to renilla luciferase activity. Relative firefly luciferase activity was reduced by miR-34a plasmid & miR-34a mimic. The experiment was repeated 3 times & triplicates were used for each analysis. Data represents the mean ± S.D. *p < 0.05. One-way ANOVA followed by post-hoc Tuckey’s test was used for analysis. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32094435), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody [NB300-320] - Hsa-miR-633 inhibitor could reverse the si-circ_0008896 phenotypes. (a) CCK8 assay was conducted to measure cell viability after up-regulation of hsa-miR-633. (b) The expression levels of two proliferation markers, Ki67 & PCNA after up-regulation of hsa-miR-633 were measured using Western blotting. (c) CCK8 assay was conducted to measure cell viability after the treatment of si-circ0008896, hsa-miR-633 inhibitor & si-circ0008896+ hsa-miR-633 inhibitor. (d) The expression levels of two proliferation markers, Ki67 & PCNA after the treatment of si-circ0008896, hsa-miR-633 inhibitor & si-circ0008896+ hsa-miR-633 inhibitor were measured using Western blotting. (e) Colony formation assay was conducted to examine proliferation ability number of colonies after the treatment of si-circ0008896, hsa-miR-633 inhibitor & si-circ0008896+ hsa-miR-633 inhibitor. (f) Quantification results of (e). (g) Transwell migration & invasion assays were conducted to examine the migration & invasion abilities of VSMCs after the treatment of si-circ0008896, hsa-miR-633 inhibitor & si-circ0008896+ hsa-miR-633 inhibitor. (h) Quantification results of (g). *P < 0.05, **P < 0.01, ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35212610), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Ca, Dr, I, Po
• Applications: WB, Simple Western, ELISA, ICC/IF, IHC, Single-Cell Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GAPDH Antibody Summary

• Immunogen: This GAPDG antibody was developed against the peptide sequence C-HQVVSSDFNSDT corresponding to C-Terminus according to NP_002037.2.
• Localization: Cytoplasmic
• Predicted Species: Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GAPDH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Simple Western
  • Single Cell Western 100 ug/ml
  • Western Blot 0.001 - 0.003 ug/mL
• Application Notes: Single Cell Western reported by an internal validation on HeLa-GFP cells at a concentration of 100 ug/ml
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  GAPDH Overexpression Lysate

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Reactivity Notes

Drosophilia reactivity reported in scientific literature (PMID: 28888970 and PMID: 33046910). Canine reactivity reported in scientific literature (PMID: 30030415). Use in Mouse reported in scientific literature (PMID:32771388).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GAPDH Antibody

• aging-associated gene 9 protein
• EC 1.2.1
• EC 1.2.1.12
• EC 2.6.99.-
• G3PD
• G3PDH
• GAPD
• GAPDH
• glyceraldehyde 3-phosphate dehydrogenase
• glyceraldehyde-3-phosphate dehydrogenase
• MGC88685
• Peptidyl-cysteine S-nitrosylase GAPDH

Background

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a ubiquitous enzyme involved in glycolysis, converting glyceraldehyde-3-phosphate into 1,3 diphosphoglycerate. This constitutively expressed, homotetramer protein can be found in the nucleus and cytoplasm, and the monomer has a theoretical molecular weight of 36 kDa. Known as a housekeeping gene, GAPDH routinely serves as a control for real-time PCR (RT-PCR) and as a loading control for Western Blots (1-3). In addition to its role in glycolysis, GAPDH has multiple cellular functions including membrane trafficking, transcription activation, apoptosis, DNA repair, and has been implicated in various pathologies such as cancer and neurodegenerative diseases including Alzheimer's and Huntington's (4,5).

References

1) Barber RD, Harmer DW, Coleman RA, Clark BJ. (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics. 21(3):389-95. PMID: 15769908

2) Jia Y, Takimoto K. (2006) Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 98(3):386-93. PMID: 16385079

3) Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 171(6):1045-59. PMID: 16365169

4) Sirover MA1. (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta. 1432(2): 159-84. PMID: 10407139

5) Tristan C, Shahani N, Sedlak TW, Sawa A. (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 23(2):317-23. PMID: 20727968

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GAPDH Antibody (NB300-320)(69)

Publications using NB300-320

• Nagasaki T, Schuyler AJ, Zhao J Et al. 15LO1 dictates glutathione redox changes in asthmatic airway epithelium to worsen type-2 inflammation The Journal of clinical investigation 2021-11-11 [PMID: 34762602] (WB, Human)
• Christoph Kuppe, Mahmoud M Ibrahim, Jennifer Kranz, Xiaoting Zhang, Susanne Ziegler, Javier Perales-Patón, Jitske Jansen, Katharina C. Reimer, James R. Smith, Ross Dobie, John R. Wilson-Kanamari, Maurice Halder, Yaoxian Xu, Nazanin Kabgani, Nadine Kaesler, Martin Klaus, Lukas Gernhold, Victor G. Puelles, Tobias B. Huber, Peter Boor, Sylvia Menzel, Remco M. Hoogenboezem, Eric M.J. Bindels, Joachim Steffens, Jürgen Floege, Rebekka K Schneider, Julio Saez-Rodriguez, Neil C Henderson, Rafael Kramann Decoding myofibroblast origins in human kidney fibrosis Nature 2021-08-23 [PMID: 33176333]
• Popp L, Segatori L, Zinc Oxide Particles Induce Activation of the LysosomeAutophagy System ACS Omega 2019-01-31 (WB, Human)
• Udayakumar PD Dissecting the Transactivation Domain of the Transcription Factor c-Myb Thesis (WB, Human)
• Mozin E, Massouridès E, Mournetas V, Lièvre C et Al. Dystrophin deficiency impairs cell junction formation during embryonic myogenesis from pluripotent stem cells iScience 2024-07-23 [PMID: 39040067]
• Chen, X;Wang, F;Tang, J;Meng, J;Han, Z; Paralemmin-3 augments lipopolysaccharide-induced acute lung injury with M1 macrophage polarization via the notch signaling pathway Respiratory physiology & neurobiology 2023-12-14 [PMID: 38103708]
• Renz C, Asimaki E, Meister C et Al. Ubiquiton-An inducible, linkage-specific polyubiquitylation tool Mol Cell 2024-01-18 [PMID: 38103558]
• Yamada T, Yoshinari Y, Tobo M et al. Nac? protects the larval fat body from cell death by maintaining cellular proteostasis in Drosophila Nature communications 2023-09-01 [PMID: 37658058] (WB, Drosophila)
• Lemma RB, Ledsaak M, Fuglerud BM et al. MYB regulates the SUMO-protease SENP1 and its novel interaction partner UXT - modulating MYB target genes and the SUMO landscape The Journal of biological chemistry 2023-07-17 [PMID: 37468105] (WB, Human)
• I Liebold, S Meyer, M Heine, A Kuhl, J Witt, L Eissing, AW Fischer, AC Koop, J Kluwe, JSZ Wiesch, M Wehmeyer, U Knippschil, L Scheja, J Heeren, L Bosurgi, A Worthmann TREM2 Regulates the Removal of Apoptotic Cells and Inflammatory Processes during the Progression of NAFLD Cells, 2023-01-17;12(3):. 2023-01-17 [PMID: 36766683]
• Winnica DE, Monzon A, Ye S et al. Airway epithelial Paraoxonase-2 in obese asthma PloS one 2022-03-14 [PMID: 35286330] (WB, Human)
• Hou X, Dai H, Zheng y Circular RNA hsa_circ_0008896 accelerates atherosclerosis by promoting the proliferation, migration and invasion of vascular smooth muscle cells via hsa-miR-633/CDC20B (cell division cycle 20B) axis Bioengineered 2022-03-01 [PMID: 35212610] (WB, Human)
• Tamargo-GOmez I, MartInez-GarcIa GG, SuArez MF et al. ATG4D is the main ATG8 delipidating enzyme in mammalian cells and protects against cerebellar neurodegeneration Cell death and differentiation 2021-04-01 [PMID: 33795848]
• Yamada T, Hironaka KI, Habara O et al. A developmental checkpoint directs metabolic remodelling as a strategy against starvation in Drosophila Nat Metab 2020-10-01 [PMID: 33046910] (Drosophila)
• Udayakumar PD Dissecting the transactivation domain (tAD) of the transcription factor c-Myb to assess recent models of tAD function FEBS Open Bio 2020-09-16 [PMID: 32937031]
• Hayashi Y, Asuzu DT, Bardsley MR Wnt-induced, TRP53-mediated Cell Cycle Arrest of Precursors Underlies Interstitial Cell of Cajal Depletion During Aging Cell Mol Gastroenterol Hepatol 2020-08-07 [PMID: 32771388] (WB, Mouse)
• Dee RA, Bai X, Mack CP, Taylor JM Molecular Regulation of the RhoGAP GRAF3 and Its Capacity to Limit Blood Pressure In Vivo Cells 2020-04-22 [PMID: 32331391] (WB, Rat)
• Hu, H, Hone, E A Et al. MiR-34a Interacts with Cytochrome c and Shapes Stroke Outcomes. Sci Rep 2020-02-24 [PMID: 32094435] (ICC/IF, Mouse)
• Krohn L, OztUrk TN, Vanderperre B et al. Genetic, structural and functional evidence link TMEM175 to synucleinopathies Ann. Neurol. 2019-10-28 [PMID: 31658403]
• Zeng Y, Bhagyashree B, Zhao W et al. Hysteretic Genetic Circuit for Detection of Proteasomal Degradation in Mammalian Cells ACS Synth Biol 2019-08-27 [PMID: 31415719]
• Paul M, Murphy S F et al. Protease-activated receptor 2 activates CRAC-mediated Ca2+ influx to cause prostate smooth muscle contraction. FASEB Bioadv 2019-01-04 [PMID: 31198907] (WB, Human)
• DeAngelis MW, Johnson RI. Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation J Vis Exp 2019-03-15 [PMID: 30933080] (WB, Drosophila)
• Zhou X, Kinlough CL, Hughey RP et al. Sialylation of MUC4b N-glycans by ST6GAL1 orchestrates human airway epithelial cell differentiation associated with Type-2 inflammation JCI Insight 2019-02-07 [PMID: 30730306] (WB, Human)
• Muta Y, Tanaka T, Hamaguchi Y et al. Selective androgen receptor modulator, S42 has anabolic and anti-catabolic effects on cultured myotubes. Biochem Biophys Rep 2019-03-01 [PMID: 30705972] (WB, Mouse)
• Patron LA, Nagatomo K, Eves DT et al. Cul4 ubiquitin ligase cofactor DCAF12 promotes neurotransmitter release and homeostatic plasticity. J. Cell Biol. 2019-01-22 [PMID: 30670470] (WB, Drosophila)
• Inoue H, Hattori T, Zhou X et al. Dysfunctional ErbB2, an EGF receptor family member, hinders repair of asthmatic airway epithelial cells. J. Allergy Clin. Immunol. 2019-01-09 [PMID: 30639343] (WB, Human)
• Heine M, Fischer AW, Schlein C et al. Lipolysis Triggers a Systemic Insulin Response Essential for Efficient Energy Replenishment of Activated Brown Adipose Tissue in Mice. Cell Metab. 2018-07-04 [PMID: 30033199] (WB, Mouse)
• Hashimoto T, Kim GE, Tunin RS et al. Acute Enhancement of Cardiac Function by Phosphodiesterase Type 1 Inhibition: A Translational Study in the Dog and Rabbit. Circulation. 2018-07-20 [PMID: 30030415] (WB, Canine)
• Ramos-Lewis W, LaFever kS, Page-McCaw A. A scar-like lesion is apparent in basement membrane after wound repair in vivo. Matrix Biol. 2018-07-05 [PMID: 29981372] (WB, Drosophila)
• Krohn L The genetics of REM sleep behavior disorder and its conversion to overt neurodegeneration Thesis (WB, Human)
• Sullivan CS, Gotthard I, Wyatt EV et al. Perineuronal Net Protein Neurocan Inhibits NCAM/EphA3 Repellent Signaling in GABAergic Interneurons. Sci Rep 2018-04-18 [PMID: 29670169] (WB, Mouse)
• Hain A, Kramer M, Linka RM et al. IL-2 Inducible Kinase ITK is Critical for HIV 1 Infection of Jurkat T-cells. Sci Rep. 2018-02-16 [PMID: 29453458] (WB, Human)
• Bushnell HL, Feiler CE, Ketosugbo KF et al. JNK is antagonized to ensure the correct number of interommatidial cells pattern the Drosophila retina. Dev. Biol. 2017-11-11 [PMID: 29133184] (WB, Drosophila)
• Hope KA, LeDoux MS, Reiter LT. Glial overexpression of Dube3a causes seizures and synaptic impairments in Drosophila concomitant with down regulation of the Na(+)/K(+) pump ATPa Neurobiol. Dis. 2017-09-06 [PMID: 28888970] (WB, Drosophila)
• Grosse S, Penaud-Budloo M, Herrmann AK et al. Relevance of Assembly-Activating Protein for Adeno-associated Virus Vector Production and Capsid Protein Stability in Mammalian and Insect Cells J. Virol. 2017-10-15 [PMID: 28768875] (WB, Insect)
• Yi H, Zhang Q, Brooks ED et al. Systemic Correction of Murine Glycogen Storage Disease Type IV by an AAV-Mediated Gene Therapy. Hum. Gene Ther. 2016-11-10 [PMID: 27832700] (WB, Mouse)
• Bell RL, Hauser S, Rodd ZA et al. A Genetic Animal Model of Alcoholism for Screening Medications to Treat Addiction. Int Rev Neurobiol 2016-03-21 [PMID: 27055615]
• Rusconi F, Grillo B, Ponzoni L et al. LSD1 modulates stress-evoked transcription of immediate early genes and emotional behavior. Proc. Natl. Acad. Sci. U.S.A. 2016-03-29 [PMID: 26976584] (WB, Mouse)
• Urraca N, Memon R, El-Iyachi I et al. Characterization of neurons from immortalized dental pulp stem cells for the study of neurogenetic disorders. Stem Cell Res. 2015-11-01 [PMID: 26599327] (WB, Human)
• Bader CA, Shandala T, Ng YS et al. Atg9 is required for intraluminal vesicles in amphisomes and autolysosomes. Biol Open 2015-09-09 [PMID: 26353861] (WB, Drosophila)
• Hijazi A, Guan H, Cernea M, Yang K. Prenatal exposure to bisphenol A disrupts mouse fetal lung development. FASEB J. 2015-08-17 [PMID: 26283537] (WB, Mouse)
  
  Details:
  GAPDH antibody (Imgenex IMG-3073) was used for WB analysis of lysates from fetal lungs of mice fed with control or bisphenol A/BPA supplemented diets (Figure 3 & 4), and or from mice also subjected to treatment of dexamethasone alongwith BPA exposure (Figure 5).
• DeBenedictis B, Guan H, Yang K et al. Prenatal Exposure to Bisphenol A Disrupts Mouse Fetal Liver Maturation in a Sex-Specific Manner J. Cell. Biochem. 2015-07-03 [PMID: 26146954] (WB, Mouse)
• Grillon E, Farion R, Reuveni M et al. Spatial profiles of markers of glycolysis, mitochondria, and proton pumps in a rat glioma suggest coordinated programming for proliferation. BMC Res Notes 2015-01-01 [PMID: 26032618] (Rat)
• Rao PS, Bell RL, Engleman EA, Sari Y. Targeting glutamate uptake to treat alcohol use disorders. Front Neurosci 2015-01-01 [PMID: 25954150]
• Obis T, Besalduch N, Hurtado E, Nadal L. The novel protein kinase C epsilon isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release Molecular Brain. 2015-01-01 [PMID: 25761522] (WB, Rat)
• Haykal M, Rodrigues-Ferreira S, Nahmias C Aneuploidy triggers vulnerability to WEE1 inhibition via severe chromosome pulverization bioRxiv 2023-09-19 (WB, Human)
• Qu H, Tu Y, Guan JL et al. Kindlin-2 Tyrosine Phosphorylation and Interaction with Src Serve as a Regulatable Switch in the Integrin Outside-in Signaling Circuit. J Biol Chem. 2014-09-18 [PMID: 25237194]
• Wang Y, Marino-Enriquez A, Bennett RR et al. Dystrophin is a tumor suppressor in human cancers with myogenic programs. Nat. Genet. 2014-06-01 [PMID: 24793134] (WB, Human, Mouse)
  
  Details:
  Human mesenchymal tumor, primary mouse interstitial cells of Cajal, and various human cell lines, Figs 3, 4 S5-S7, S9. The GADPH pAb (1:10,000) was used as a loading control to standardize protein loading.
• Rusconi F, Paganini L, Braida D et al. LSD1 Neurospecific Alternative Splicing Controls Neuronal Excitability in Mouse Models of Epilepsy. Cereb. Cortex 2014-04-15 [PMID: 24735673] (WB, Mouse)
• Renz C, Asimaki E, Meister C et al. Ubiquiton - An Inducible, Linkage-Specific Polyubiquitylation Tool Available at SSRN 2023-04-18 (WB, Human)
• Yi H, Brooks ED, Thurberg BL et al. Correction of glycogen storage disease type III with rapamycin in a canine model. J. Mol. Med. 2014-02-10 [PMID: 24509886] (WB, Canine)
• McLelland GL, Soubannier V, Chen CX, Fon EA. Parkin and PINK1 function in a vesicular trafficking pathway regulating mitochondrial quality control. EMBO J. 2014-02-21 [PMID: 24446486] (WB, Human)
• Louvet E, Yoshida A, Kumeta M et al. Probing the stiffness of isolated nucleoli by atomic force microscopy. Histochem Cell Biol 2013-12-03 [PMID: 24297448] (WB, Human)
• Duncan JE, Lytle NK, Zuniga A, Goldstein LS. The Microtubule Regulatory Protein Stathmin Is Required to Maintain the Integrity of Axonal Microtubules in Drosophila. PLoS One. 2013-06-26 [PMID: 23840848]
• Yi H, Fredrickson KB, Das S et al. Stbd1 is highly elevated in skeletal muscle of Pompe disease mice but suppression of its expression does not affect lysosomal glycogen accumulation. Mol Genet Metab 2013-05-18 [PMID: 23726947] (WB, Mouse)
• Lee YY, Yu YB, Gunawardena HP et al. BCLAF1 is a radiation-induced H2AX-interacting partner involved in GH2AX-mediated regulation of apoptosis and DNA repair. Cell Death Dis. 2012-07-26 [PMID: 22833098]
• Nechipurenko IV, Broihier HT. FoxO limits microtubule stability and is itself negatively regulated by microtubule disruption. J Cell Biol. 2012-02-06 [PMID: 22312004] (WB)
  
  Details:
  GAPDH (IMG-3073). WB: Drosophila cells with various mutations: Figs 1E, 6k, 8, & 9.
• Georges E, Bonneau A-M, Prinos P. RNAi-mediated knockdown of [alpha]-enolase increases the sensitivity of tumor cells to antitubulin chemotherapeutics. Int J Biochem Mol Biol 2(4):303-308. 2011-01-01 [PMID: 22187664]
• Noelker C, Schwake M, Balzer-Geldsetzer M et al. Differentially expressed gene profile in the 6-hydroxy-dopamine-induced cell culture model of Parkinson's disease. Neurosci Lett 2011-12-01 [PMID: 22155091]
• Weile C, Josefsen K, Buschard K. Glucose activation of islets of Langerhans up-regulates Toll-like receptor 5: possible mechanism of protection. Clin Exp Immunol. 2011-11-01 [PMID: 21985371] (WB, Rat)
  
  Details:
  TLR4/CD284 (IMG-5031A) and GAPDH (IMG-3073). WB: Cultured rat islet Langerhans treated with glucose, Fig 1B. Note: TLR4 protein levels were not increased in the presence of glucose activation, Fig 1B.
• Pulinilkunnil T, He H, Kong D et al. Adrenergic regulation of AMP-activated protein kinase in brown adipose tissue in vivo. J Biol Chem. 2011-03-18 [PMID: 21209093] (WB, Mouse)
  
  Details:
  WB: GAPDH Loading Control (IMG-3073). Mouse interscapular brown adipose cells, Fig 3A.
• Besalduch N, Tomas M, Santafe MM et al. Synaptic activity-related classical protein kinase C isoform localization in the adult rat neuromuscular synapse. J Comp Neurol. 2010-01-10 [PMID: 19937712] (WB, Rat)
  
  Details:
  GAPDH Loading Control (IMG-3073). WB: Levat or auris longus (LAL) rat muscle, Fig 5B.
• Carr AJ, Vugler A, Lawrence J, Chen LL, Ahmado A, Chen FK, Semo M, Gias C, da Cruz L, Moore HD, Walsh J, Coffey PJ. Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay. Mol Vis;15:283-95. 2009-01-01 [PMID: 19204785]
• Glasheen BM, Kabra AT, Page-McCaw A. Distinct functions for the catalytic and hemopexin domains of a Drosophila matrix metalloproteinase. Proc Natl Acad Sci U S A. 2009-02-24 [PMID: 19196956] (WB)
  
  Details:
  WB: Fig 4A (drosophila embryos), Fig 4B (drosophila larvae). Note: GAPDH was used as a loading control. Differential expression of GAPDH isoforms were seen in embryos (one GAPDH band between 36 and 50 kDa) compared to larvae (two GAPDH bands between 31 an
• Kiepe D, Van Der Pas A, Ciarmatori S, Standker L, Schutt B, Hoeflich A, Hugel U, Oh J, Tonshoff B. Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein 2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I. Endocrinology;149(10):4901-11. 2008-10-01 [PMID: 18556354]
• Henson MA, Roberts AC, Salimi K et al. Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex. Cereb Cortex. 2008-11-01 [PMID: 18296432] (WB, Mouse)
  
  Details:
  WB (mouse frontal cortex lysates), Fig. 1.
• Gerstner JR, Bremer QZ, Vander Heyden WM et al. Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain. PLoS One. 2008-02-20 [PMID: 18286188] (WB, Mouse)
• Nguyen M, Pace AJ, Koller BH. Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia. J Exp Med;204(6):1383-93. 2007-06-11 [PMID: 17517966] (WB, Mouse)
  
  Details:
  WB, mouse lung tissue lysates (used as a protein loading control): Fig 8.
• Burke JR, Enghild JJ, Martin ME et al. Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH. Nat Med 1996-03-01 [PMID: 8612237]

Reviews for GAPDH Antibody (NB300-320) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer WB Human 08/19/2014

Summary

• Application: Western Blot
• Sample Tested: 293T cells
• Species: Human

reviewed by: Verified Customer WB Mouse 08/18/2014

Summary

• Application: Western Blot
• Sample Tested: Mouse Triceps and Rectus Abdominis
• Species: Mouse

FAQs for GAPDH Antibody (NB300-320). (Showing 1 - 7 of 7 FAQs).

1. We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?  ..show answer..
   • GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.
2. I want to know why GAPDH is used as a loading control antibody in WB techniques.  ..show answer..
   • Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.
3. I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?  ..show answer..
   • This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.
4. I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?  ..show answer..
   • Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.
5. What secondary antibody should I use for visualization of the GAPDH antibody?  ..show answer..
   • This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.
6. Do you offer a smaller size for the GAPDH antibodies?  ..show answer..
   • We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.
7. I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.  ..show answer..
   • For homogenized tissue, beta-actin and GAPDH are both fine.

Bioinformatics

• Gene Symbol: GAPDH
• Entrez:
  • 2597(Human)
  • 14433(Mouse)
  • 24383(Rat)
• Uniprot:
  • NP_002037.2(Human)