GATA-3 [p Ser308] Antibody (1E5) - BSA Free

Immunohistochemistry: GATA-3 [p Ser308] Antibody (1E5) [NBP3-26423] - Image of GATA-3 [p Ser308] Antibody (1E5) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot: GATA-3 [p Ser308] Antibody (1E5) [NBP3-26423] - Positive Western Blot detected in Hela whole cell lysate, A549 whole cell lysate. All lanes: GATA-3 [p Ser308] Antibody at 2.5ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48 KDa Observed band size: 48 KDa

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 1E5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GATA-3 [p Ser308] Antibody (1E5) - BSA Free Summary

• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human GATA-3 [p Ser308] [UniProt P23771]
• Modification: p Ser308
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GATA3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for GATA-3 [p Ser308] Antibody (1E5) - BSA Free

• 131320
• GATA binding protein 3
• GATA3
• GATA-3
• GATA-binding factor 3
• GATA-binding protein 3
• HDRMGC5199
• MGC2346
• MGC5445
• trans-acting T-cell-specific transcription factor GATA-3

Background

The GATA3 gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains twoGATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelialcell biology. Defects in th

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GATA3