GDNF Antibody

Product Discontinued

GDNF Antibody Summary

• Immunogen: Synthetic peptide surrounding amino acid 195 of mouse GDNF (BAA08660).
• Specificity: The antibody recognizes GDNF of human, mouse, and rat origins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GDNF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 10 - 20 ug/ml
  • Immunoprecipitation 10 - 20 ug/ml
  • Western Blot 0.5 - 4 ug/ml
• Application Notes: Jurkat cell lysate can be used as a positive control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.5% BSA, 30% Glycerol
• Preservative: 0.01% Thimerosal
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GDNF Antibody

• Astrocyte-derived trophic factor
• ATF
• ATF1
• ATF2
• GDNF
• glial cell derived neurotrophic factor
• glial cell line derived neurotrophic factor
• glial cell line-derived neurotrophic factor
• HFB1-GDNF
• HGDNF
• HSCR3

Background

This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for GDNF Antibody (NBP1-45595)(4)

Publications using NBP1-45595

• Li H, Fan C, Lu H et al. Protective role of berberine on ulcerative colitis through modulating enteric glial cells-intestinal epithelial cells-immune cells interactions Acta Pharm Sin B 2020-03-07 [PMID: 32140391]
• N'Tumba-Byn T, Yamada M, Seandel M Loss of tyrosine kinase receptor Ephb2 impairs proliferation and stem cell activity of spermatogonia in culture Biol. Reprod. 2019-12-13 [PMID: 31836902]
• Naudin C, Smith B, Bond DR et al. Characterization of the early molecular changes in the glomeruli of Cd151 -/- mice highlights induction of mindin and MMP-10 Sci Rep. 2017-11-22 [PMID: 29167507] (ICC/IF, Mouse)
• Li Yan-Hua, Xie Chong, Zhang Yuan et al. FSD-C10, a Fasudil derivative, promotes neuroregeneration through indirect and direct mechanisms. Sci Rep 2017-01-01 [PMID: 28112256] (IF/IHC, Mouse)

FAQs for GDNF Antibody (NBP1-45595). (Showing 1 - 1 of 1 FAQs).

1. Our customer want to know the MW size for WB. Could you check and let me know about it?
   • This antibody recognizes a band around 15kDa on a Western blot.

Bioinformatics

• Gene Symbol: GDNF
• Entrez:
  • 2668(Human)
  • 14573(Mouse)
  • 25453(Rat)
• Uniprot:
  • BAA08660(Mouse)