GITR Ligand/TNFSF18 Antibody (721926)

Western blot shows lysates of A20 mouse B cell lymphoma cell line and BaF3 mouse pro-B cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse GITR Ligand/TNFSF18 Monoclonal Antibody (Catalog # MAB21772) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for GITR Ligand/TNFSF18 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clone: 721926
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

GITR Ligand/TNFSF18 Antibody (721926) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse GITR Ligand/TNFSF18
  Thr47-Ser173
  Accession # NP_899247
• Specificity: Detects mouse GITR Ligand/TNFSF18 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) APRIL, rmBAFF, rmEDA, rmFas Ligand, rmLIGHT, rmOX40 Ligand, rmTNF-alpha , rmTRAIL, rmTRANCE, rmTWEAK, rm4-1BB Ligand, recombinant human (rh) EDA-A2, rhLTa1/b2, rhLTa2/b1, rhVEGI, or rhGITR Ligand is observed. In Western blots, no cross-reactivity with rm4‑1BB, rmEDA, rmLIGHT, rmTRAIL, rhGITR Ligand, rhTNF-alpha , recombinant canine TNF‑ alpha , recombinant guinea pig TNF-alpha , or recombinant porcine TNF-alpha is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: TNFSF18
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GITR Ligand/TNFSF18 Antibody (721926)

• Activation-inducible TNF-related ligand
• AITRL
• AITRLMGC138237
• GITR Ligand
• GITRL
• GITRLglucocorticoid-induced TNFR-related protein ligand
• Glucocorticoid-induced TNF-related ligand
• hGITRLGITR ligand
• TL6AITR ligand
• TNFSF18
• tumor necrosis factor (ligand) superfamily, member 18
• tumor necrosis factor ligand superfamily member 18

Background

Glucocorticoid-induced TNF receptor superfamily-related protein ligand (GITRL) is a member of the TNF superfamily (TNFSF) and has been designated TNFSF18. Mouse GITRL cDNA encodes a 173 amino acid (aa) type II membrane protein with a C-terminal extracellular domain of 131 aa, an N-terminal cytoplasmic domain of 23 aa and a transmembrane domain of 19 aa. It shares approximately 60% aa sequence identity with human GITRL (2). Mouse GITRL is expressed at high levels in macrophages, dendritic cells and B cells. The expression is transiently upregulated by LPS stimulation. GITRL binds to the type I transmembrane protein GITR/TNFRSF18, which is a member of the TNF receptor superfamily that is predominantly expressed on CD25⁺ regulatory CD4⁺ T cells (Treg). GITR is also expressed on naïve CD4⁺ CD25^- T cells, where its expression is upregulated after antigen-driven activation (1, 2). Ligation of GITR has been found to induce nuclear factor (NF)-kappa B activation via TNF receptor-associated factor 2. GITRL provides costimulatory signals for activated CD4⁺ CD25^- T cells to enhance cell proliferation and augment cytokine production. On CD4⁺ CD25⁺ Treg cells, GITRL also provides costimulatory signals to induce proliferation, setting Treg cells in an active/hyperproliferactive state that reverses the suppressive function of Treg cells. GITRL-GITR ligation provides important costimulatory signals that play important roles in modulating diverse T cell functions (1-4).

1. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
2. Ji, H. et al. (2004) J. Immunology 172:5823.
3. Kanamaru, F. et al. (2004) J. Immunology 172:7306.
4. Ronchetti, S. et al. (2004) Eur. J. Immunology 34:613.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GITR Ligand/TNFSF18 Antibody (MAB21772)(2)

Publications using MAB21772

• Yisel Rivera-Molina, Hong Jiang, Juan Fueyo, Teresa Nguyen, Dong Ho Shin, Gilbert Youssef et al. GITRL-armed Delta-24-RGD oncolytic adenovirus prolongs survival and induces anti-glioma immune memory Neuro-Oncology Advances 2019-10-15 [PMID: 31608328]
• Kanagavelu , Saravana, Termini , James M, Gupta , Sachin, Raffa , Francesc, Fuller , Katherin, Rivas , Yaelis, Philip , Sakhi, Kornbluth , Richard, Stone , Geoffrey HIV-1 adenoviral vector vaccines expressing multi-trimeric BAFF and 4-1BBL enhance T cell mediated anti-viral immunity. PLoS ONE, 2014-02-28;9(2):e90100. 2014-02-28 [PMID: 24587225] (Western Blot, Human)

Bioinformatics

• Gene Symbol: TNFSF18
• Uniprot:
  • NP_899247()