GLI-2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with GLI-2 Antibody (NBP2-23602) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: GLI-2 Antibody [NBP2-23602] - GLI-2 (red) and DAPI (blue) in mouse BT-474 primary tumors (breast cancer tissue). IHC-P image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - GLI-2 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody dilution of 1:50 was used. Image objective 40x.

Immunohistochemistry: GLI-2 Antibody [NBP2-23602] - Analysis of GLI-2 in mouse heart.

• Reactivity: Hu, Mu, Rt, Ha
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GLI-2 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal portion of the human GLI-2 protein (between residues 300-400) [UniProt# P10070]
• Localization: Nuclear and cytoplasm
• Predicted Species: Hamster (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GLI2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:200 - 1:300
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 26210874)
  • Immunohistochemistry-Paraffin 1:200 - 1:300
• Application Notes: In Immunocytochemistry/Immunofluorescence, cytoplasmic and nuclear staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 26210874).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GLI-2 Antibody - BSA Free

• GLI family zinc finger 2
• GLI2
• GLI-2
• GLI-Kruppel family member GLI2
• glioma-associated oncogene family zinc finger 2
• HPE9tax helper protein 1
• oncogene GLI2
• tax helper protein 2
• Tax helper protein
• tax-responsive element-2 holding protein
• tax-responsive element-25-bp sequence binding protein
• THP
• THP1
• THP2
• zinc finger protein GLI2

Background

Gli2, also known as THP, is a transcription factor which binds DNA through zinc finger motifs. Belonging to the C2H2-Zinc finger type subclass of the Gli family, Gli2 is a mediator of Shh (sonic hedgehog) signaling, a pathway that is aberrantly activated in many types of cancers. Specifically, it has been found that the anti-apoptotic protein BCL-2 is up regulated by Gli2, leading to carcinogenesis. The protein encoded by the Gli2 gene localizes to the cytoplasm and activates patched (Drosophila homolog) gene expression, where it has been thought to play an important role during embryogenesis. Mutations in the Gli2 gene are found in various syndromes, including Pallister-Hall Syndrome, Greig cephalopolysyndactyly syndrome, and preaxial polydactyl type IV. Recently, the interaction between the hedgehog signaling pathway and Gli2 has been implicated in medullary thyroid carcinoma (PMID: 23410206).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GLI-2 Antibody (NBP2-23602)(3)

Publications using NBP2-23602

• Chen J, Cheng NC, Boland JA Et al. Deletion of kif3a in CK19 positive cells leads to primary cilia loss, biliary cell proliferation and cystic liver lesions in TAA-treated mice Biochim Biophys Acta Mol Basis Dis 2022-01-01 [PMID: 34973373] (IF/IHC, Mouse)
  
  Details:
  Citation using the Alexa Fluor 647 version of this antibody.
• Chakrabarti J, Holokai L, Syu L et al. Hedgehog signaling induces PD-L1 expression and tumor cell proliferation in gastric cancer. Oncotarget 2018-12-21 [PMID: 30647844] (Immunohistochemistry-Frozen, Rat)
• Martinez JA, Kobayashi M, Krishnan A et al. Intrinsic facilitation of adult peripheral nerve regeneration by the Sonic hedgehog morphogen Exp. Neurol. 2015-07-22 [PMID: 26210874] (IHC-Fr, Rat)

Review for GLI-2 Antibody (NBP2-23602) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-P Mouse 08/17/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Breast cancer tissue
• Species: Mouse
• Lot: A-3

Comments

• Comments: Citrate retrieval buffer

Bioinformatics

• Gene Symbol: GLI2
• Entrez:
  • 2736(Human)
  • 14633(Mouse)
• Uniprot:
  • P10070()
  • Q0VGT2(Mouse)