GLI-3 Antibody (JG83-39)

Western Blot: GLI-3 Antibody (JG83-39) [NBP2-75506] - Analysis of Gli3 on PC-3M cell lysate using anti-Gli3 antibody at 1/1,000 dilution.

Product Discontinued

GLI-3 Antibody (JG83-39) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human GLI-3 aa 1261-1580 / 1580. (SwissProt: P10071 Human)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GLI3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Western Blot 1:500-1:2000
• Theoretical MW: 190 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GLI-3 Antibody (JG83-39)

• ACLS
• ACLSPAPAPHS
• ADD
• Bph
• GCPS
• GLI family zinc finger 3
• GLI3 form of 190 kDa
• GLI3 full length protein
• GLI3
• GLI-3
• GLI3-190
• GLI3FL
• GLI-Kruppel family member GLI3
• glioma-associated oncogene family zinc finger 3
• Greig cephalopolysyndactyly syndrome
• oncogene GLI3
• PAP-A
• PAPA1
• PAPB
• PAPBDNA-binding protein
• Pdn
• PHS
• PPDIV
• Xt
• zinc finger protein GLI3

Background

Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-polysyndactyly syndrome (GCPS); an autosomal dominant disorder-affecting limb and craniofacial development. Two isoforms of human Gli-3 have been reported. One is the fulllength protein at ~170-190kDa and the other is a truncated isoform at ~80kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLI3