GLI-3 Antibody

Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GLI-3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GLI-3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI‑3/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse GLI‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The gli-1 promoter was detected by standard PCR.

GLI-3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Conditional deletion of GATA6 does not significantly alter Gli3 processing in the hindlimb bud.(A) Western blot analysis of Gli3FL and Gli3R steady state levels in anterior (lanes 1 and 3) or posterior (lanes 2 and 4) halves of E11.5 hindlimb buds isolated from either control (lanes 1 and 2) or Prx1-Cre; GATA6fl/fl (lanes 3 and 4) mouse embryos. alpha –Tubulin is used as a loading control. (B) Quantification of Gli3FL/Gli3R ratios (by densitometry of Western Blots) in anterior or posterior halves of E11.5 hindlimb buds isolated from either littermate controls (lanes 1 and 2) or Prx1-Cre; GATA6fl/fl (lanes 3 and 4) animals, 12 embryos of each genotype were analyzed. Error bar indicates standard deviation, n = 12. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24415953), licensed under a CC-BY license. Not internally tested by R&D Systems.

SufuT396I does not stabilize Gli3FL protein and reduces the processing of Gli3FL.(A) Western blotting of lysates prepared from SufuR146X/R146X at E9.5 and SufuT396I/T396I, SufuT396I/+, and wild-type embryos at E10.5 with anti-Gli3, anti-Sufu, and anti-actin antibodies. Each image presented in the Fig. is a representative of independent triplicated experiments. The full gel images are shown in S6A Fig. (B, C) Relative expression of Gli3FL (B) and Gli3REP (B and C). Western blotting was performed two times using lysates prepared from five wild-type and five SufuT396I/T396I embryos at E10.5 (S2A Fig.). Expression levels were quantified from the band intensity shown in S2A Fig. as relative values of the Gli3FL/actin and Gli3REP/actin expression ratios (B) and the direct ratio of Gli3REP/Gli3FL (C). (**) p < 0.01, two-tailed Student’s t-test. Error bars indicate the standard deviations. (D) Western blotting of cell lysates from wild-type and SufuT396I/T396I MEFs with indicated antibodies. The SufuT396I/T396I MEFs were electroporated with 10.0, 1.0, or 0.1 μg of the HA–Sufu construct (lane 3, 4, and 5, respectively), or 10 μg of the HA–SufuT396I construct (lane 6). The mobilities on SDS-PAGE of the wild-type Sufu (lane 3–5) and SufuT396I (lane 6) are identical. The complete gel images are shown in S6B Fig. This image is representative of two independent experiments. (E) Western blotting of cell lysates from Sufu−/− cells with indicated antibodies. The Sufu−/− cells were electroporated with a mixture of the Flag–Gli3 construct (6 μg) and the HA–Sufu construct (4.00, 1.33, 0.44, or 0.15 μg) for the wild-type Sufu cotransfection (lane 2 to 5, respectively), or a mixture of the Flag–Gli3 construct (6 μg) and the HA–SufuT396I construct (4 μg) for mutant Sufu cotransfection (lane 6). The complete gel images are shown in S6C Fig. This image is representative of two independent experiments. (F) Western blotting of immunoprecipitates or lysates from 293T cells transfected with expression constructs as indicated at the top. The complete gel images are shown in S6D Fig. This image is representative of two independent experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25760946), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu
• Applications: WB, ChIP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

GLI-3 Antibody Summary

• Immunogen: E. coli-derived recombinant human GLI-3
  Met1-Glu479
  Accession # P10071
• Specificity: Detects human and mouse GLI-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GLI3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GLI-3 Antibody

• ACLS
• ACLSPAPAPHS
• ADD
• Bph
• GCPS
• GLI family zinc finger 3
• GLI3 form of 190 kDa
• GLI3 full length protein
• GLI3
• GLI-3
• GLI3-190
• GLI3FL
• GLI-Kruppel family member GLI3
• glioma-associated oncogene family zinc finger 3
• Greig cephalopolysyndactyly syndrome
• oncogene GLI3
• PAP-A
• PAPA1
• PAPB
• PAPBDNA-binding protein
• Pdn
• PHS
• PPDIV
• Xt
• zinc finger protein GLI3

Background

Human GLI-3 is a 190 kDa transcription factor of the C2H2-type zinc-finger protein family. GLI-3 appears to play a role in limb and brain development, probably through sonic hedgehog signaling. GLI-3 is regulated by PKA-mediated phosphorylation. Unphosphorylated full-length GLI-3 is a transcriptional activator, while phosphorylated GLI-3 is cleaved after amino acid 700 to generate an N-terminal 83 kDa transcriptional repressor. Over the region used as immunogen, human GLI-3 is 95% identical to the corresponding mouse and canine protein sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GLI-3 Antibody (AF3690)(101)

Publications using AF3690

• U?pie?ski, T;Niewiadomski, P; The Proteasome and Cul3-Dependent Protein Ubiquitination Is Required for Gli Protein-Mediated Activation of Gene Expression in the Hedgehog Pathway Cells 2024-09-06 [PMID: 39273066] (Western Blot, Mouse)
• Baran, B;Derua, R;Janssens, V;Niewiadomski, P; PP2A phosphatase regulatory subunit PPP2R3C is a new positive regulator of the hedgehog signaling pathway Cellular signalling 2024-08-20 [PMID: 39173855] (Western Blot, Human)
• Zhonghao Deng, Shengwei Rong, Lu Gan, Fuhua Wang, Liangxiao Bao, Fang Cai et al. Temporal transcriptome features identify early skeletal commitment during human epiphysis development at single-cell resolution iScience 8/18/2023 [PMID: 37554462]
• Meropi Bagka, Hyeonyi Choi, Margaux Héritier, Hanna Schwaemmle, Quentin T. L. Pasquer, Simon M. G. Braun et al. Targeted protein degradation reveals BET bromodomains as the cellular target of Hedgehog pathway inhibitor-1 Nature Communications 2023-07-01 [PMID: 37393376]
• P Horák, K Kreisinger, J Réda, L Ondrušová, J Balko, J Vachtenhei, P Žáková, J Vachtenhei The Hedgehog/GLI signaling pathway activates transcription of Slug (Snail2) in melanoma cells Oncology reports, 2023-03-03;49(4):. 3/3/2023 [PMID: 36866769]
• JS Fleck, SMJ Jansen, D Wollny, F Zenk, M Seimiya, A Jain, R Okamoto, M Santel, Z He, JG Camp, B Treutlein Inferring and perturbing cell fate regulomes in human brain organoids Nature, 2022-10-05;0(0):. 2022-10-05 [PMID: 36198796] (Bioassay, CUT&amp;Tag, IHC, Human)
• M Kurtovi?, N Piteša, N Bartoni?ek, P Ozreti?, V Musani, J ?onkaš, T Petri?, C King, M Sabol RNA-seq and ChIP-seq Identification of Unique and Overlapping Targets of GLI Transcription Factors in Melanoma Cell Lines Cancers, 2022-09-19;14(18):. 2022-09-19 [PMID: 36139698] (ChIP, Human)
• Megan Rowton, Carlos Perez-Cervantes, Suzy Hur, Jessica Jacobs-Li, Emery Lu, Nikita Deng et al. Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages Developmental Cell 2022-09-14 [PMID: 36108627]
• Jianjian Zhu, Rashmi Patel, Anna Trofka, Brian D. Harfe, Susan Mackem Sonic hedgehog is not a limb morphogen but acts as a trigger to specify all digits in mice Developmental Cell 2022-09-01 [PMID: 35977544]
• S Schmidt, MD Luecken, D Trümbach, S Hembach, KM Niedermeie, N Wenck, K Pflügler, C Stautner, A Böttcher, H Lickert, C Ramirez-Su, R Ahmad, MJ Ziller, JC Fitzgerald, V Ruf, WDJ van de Ber, AJ Jonker, T Gasser, B Winner, J Winkler, DM Vogt Weise, F Giesert, FJ Theis, W Wurst Primary cilia and SHH signaling impairments in human and mouse models of Parkinson&#039;s disease Nature Communications, 2022-08-16;13(1):4819. 2022-08-16 [PMID: 35974013] (IHC, Human)
• Leah Schembs, Ariane Willems, Kerstin Hasenpusch-Theil, James D. Cooper, Katie Whiting, Karen Burr et al. The ciliary gene INPP5E confers dorsal telencephalic identity to human cortical organoids by negatively regulating Sonic hedgehog signaling Cell Reports 2022-05-17 [PMID: 35584663] (Western Blot, Human)
• Juuri E, Tikka P, Domanskyi A et al. Ptch2 is a Potential Regulator of Mesenchymal Stem Cells Frontiers in Physiology 2022-04-28 [PMID: 35574464]
• Skylar I. Dewees, Romana Vargová, Katherine R. Hardin, Rachel E. Turn, Saroja Devi, Joshua Linnert et al. Phylogenetic profiling and cellular analyses of ARL16 reveal roles in traffic of IFT140 and INPP5E Molecular Biology of the Cell 2022-04-01 [PMID: 35196065]
• J Sun, J Yoon, M Lee, HK Lee, YS Hwang, IO Daar Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal development Cell Reports, 2022-02-01;38(5):110312. 2022-02-01 [PMID: 35108539] (Immunoprecipitation, Western Blot, Human)
• D Sharma, AJ Mirando, A Leinroth, JT Long, CM Karner, MJ Hilton HES1 is a novel downstream modifier of the SHH-GLI3 Axis in the development of preaxial polydactyly PloS Genetics, 2021-12-20;17(12):e1009982. 2021-12-20 [PMID: 34928956] (Western Blot, Mouse, Transgenic Mouse)
• Rachel E. Turn, Yihan Hu, Skylar I. Dewees, Narra Devi, Michael P. East, Katherine R. Hardin et al. The ARF GAPs ELMOD1 and ELMOD3 act at the Golgi and cilia to regulate ciliogenesis and ciliary protein traffic Molecular Biology of the Cell 2022-02-01 [PMID: 34818063]
• Anna Trofka, Bau-Lin Huang, Jianjian Zhu, William F. Heinz, Valentin Magidson, Yuki Shibata et al. Genetic basis for an evolutionary shift from ancestral preaxial to postaxial limb polarity in non-urodele vertebrates Current Biology 2021-11-01 [PMID: 34610275]
• Karen E. Boschen, Eric W. Fish, Scott E. Parnell Prenatal alcohol exposure disrupts Sonic hedgehog pathway and primary cilia genes in the mouse neural tube Reproductive Toxicology 2021-10-01 [PMID: 34492310]
• Yingyi Z, Jielu H, Mariana T et al. FBF1 deficiency promotes beiging and healthy expansion of white adipose tissue. Cell Rep. 2021-08-03 [PMID: 34348145]
• Sun-Hee Hwang, Bandarigoda N Somatilaka, Kevin White, Saikat Mukhopadhyay Ciliary and extraciliary Gpr161 pools repress hedgehog signaling in a tissue-specific manner eLife 2021-08-04 [PMID: 34346313]
• Ching-Fang Chang, Kari M. Brown, Yanfen Yang, Samantha A. Brugmann Centriolar Protein C2cd3 Is Required for Craniofacial Development Frontiers in Cell and Developmental Biology 2021-06-15 [PMID: 34211969]
• Chuan Chen, Qingwen Xu, Yuxia Zhang, Brian A. Davies, Yan Huang, David J. Katzmann et al. Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKI gamma Science Advances 2021-06-25 [PMID: 34162535]
• Truong ME, Bilekova S, Choksi SP et al. Vertebrate cells differentially interpret ciliary and extraciliary cAMP Cell 4/27/2021 [PMID: 33932338]
• A Koeniger, A Brichkina, I Nee, L Dempwolff, A Hupfer, I Galperin, F Finkernage, A Nist, T Stiewe, T Adhikary, W Diederich, M Lauth Activation of Cilia-Independent Hedgehog/GLI1 Signaling as a Novel Concept for Neuroblastoma Therapy Cancers, 2021-04-15;13(8):. 2021-04-15 [PMID: 33921042] (Western Blot, Mouse)
• Elena A. May, Marian Kalocsay, Inès Galtier D’Auriac, Patrick S. Schuster, Steven P. Gygi, Maxence V. Nachury et al. Time-resolved proteomics profiling of the ciliary Hedgehog response Journal of Cell Biology 2021-05-03 [PMID: 33856408]
• Rachel E. Turn, Joshua Linnert, Eduardo D. Gigante, Uwe Wolfrum, Tamara Caspary, Richard A. Kahn Roles for ELMOD2 and Rootletin in ciliogenesis Molecular Biology of the Cell 2021-04-15 [PMID: 33596093]
• KH Elliott, X Chen, J Salomone, P Chaturvedi, PA Schultz, SK Balchand, JD Servetas, A Zuniga, R Zeller, B Gebelein, MT Weirauch, KA Peterson, SA Brugmann Gli3 utilizes Hand2 to synergistically regulate tissue-specific transcriptional networks Elife, 2020-10-02;9(0):. 2020-10-02 [PMID: 33006313] (Western Blot, Mouse)
• JM Dias, Z Alekseenko, A Jeggari, M Boareto, J Vollmer, M Kozhevniko, H Wang, MP Matise, A Alexeyenko, D Iber, J Ericson A Shh/Gli-driven three-node timer motif controls temporal identity and fate of neural stem cells Science Advances, 2020-09-16;6(38):. 2020-09-16 [PMID: 32938678] (ICC, Immunoprecipitation, Western Blot, Mouse)
• Kerstin Hasenpusch-Theil, Christine Laclef, Matt Colligan, Eamon Fitzgerald, Katherine Howe, Emily Carroll et al. A transient role of the ciliary gene Inpp5e in controlling direct versus indirect neurogenesis in cortical development eLife 2020-08-25 [PMID: 32840212]
• Saishu Yoshida, Katsuhiko Aoki, Ken Fujiwara, Takashi Nakakura, Akira Kawamura, Kohji Yamada et al. The novel ciliogenesis regulator DYRK2 governs Hedgehog signaling during mouse embryogenesis eLife 2020-08-06 [PMID: 32758357]
• Fabian Freisleben, Lena Behrmann, Vanessa Thaden, Jana Muschhammer, Carsten Bokemeyer, Walter Fiedler et al. Downregulation of GLI3 Expression Mediates Chemotherapy Resistance in Acute Myeloid Leukemia International Journal of Molecular Sciences 2020-07-18 [PMID: 32708452]
• Bandarigoda Nipunika Somatilaka, Sun-Hee Hwang, Vivek Reddy Palicharla, Kevin Andrew White, Hemant Badgandi, John Michael Shelton et al. Ankmy2 Prevents Smoothened-Independent Hyperactivation of the Hedgehog Pathway via Cilia-Regulated Adenylyl Cyclase Signaling Developmental Cell 2020-09-01 [PMID: 32702291] (Western Blot, Transgenic Mouse)
• RU Chottekala, S Kalik, J Gresack, A Ayala, M Gao, W Wang, S Meller, A Aly, A Schaefer, P Greengard AP-1 controls the p11-dependent antidepressant response Mol. Psychiatry, 2020-05-21;0(0):. 2020-05-21 [PMID: 32439846] (IHC, C. elegans)
• H Yan, C Chen, H Chen, H Hong, Y Huang, K Ling, J Hu, Q Wei TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating Nat Commun, 2020-05-04;11(1):2196. 2020-05-04 [PMID: 32366837] (IHC, C. elegans)
• Y Kim, J Lee, M Seppala, MT Cobourne, SH Kim Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration Nat Commun, 2020-04-24;11(1):1994. 2020-04-24 [PMID: 32332736] (IHC, Mouse)
• Marisa E. Hom, Alison E. Ondrus, Tomoyo Sakata‐Kato, Paul G. Rack, James K. Chen Bicyclic Imidazolium Inhibitors of Gli Transcription Factor Activity ChemMedChem 2020-06-17 [PMID: 32268014]
• Katherine Q Chen, Naoyuki Tahara, Aaron Anderson, Hiroko Kawakami, Sho Kawakami, Ryuichi Nishinakamura et al. Development of the Proximal-Anterior Skeletal Elements in the Mouse Hindlimb Is Regulated by a Transcriptional and Signaling Network Controlled by Sall4 Genetics 2020-05-01 [PMID: 32156750]
• Eduardo D Gigante, Megan R Taylor, Anna A Ivanova, Richard A Kahn, Tamara Caspary ARL13B regulates Sonic hedgehog signaling from outside primary cilia eLife 2020-03-04 [PMID: 32129762]
• P Liu, M Dodson, D Fang, E Chapman, DD Zhang NRF2 negatively regulates primary ciliogenesis and hedgehog signaling PLoS Biol., 2020-02-13;18(2):e3000620. 2020-02-13 [PMID: 32053600] (Western Blot, Mouse)
• Sandii Constable, Alyssa B. Long, Katharine A. Floyd, Stéphane Schurmans, Tamara Caspary The ciliary phosphatidylinositol phosphatase Inpp5e plays positive and negative regulatory roles in Shh signaling Development 2020-01-01 [PMID: 31964774]
• MF Bastida, R Pérez-Góme, A Trofka, J Zhu, A Rada-Igles, R Sheth, HS Stadler, S Mackem, MA Ros The formation of the thumb requires direct modulation of Gli3 transcription by Hoxa13 Proc. Natl. Acad. Sci. U.S.A., 2020-01-02;0(0):. 2020-01-02 [PMID: 31896583] (Western Blot, Mouse)
• EZM Taroc, A Naik, JM Lin, NB Peterson, DL Keefe, E Genis, G Fuchs, R Balasubram, PE Forni Gli3 regulates vomeronasal neurogenesis, olfactory ensheathing cell formation and GnRH-1 neuronal migration J. Neurosci., 2019-11-25;0(0):. 2019-11-25 [PMID: 31767679] (IHC, Mouse)
• A Martin-Hur, R Martin-Mor, N Robledinos, R Blanco, I Palacios-B, I Lastres-Be, A Cuadrado, FR Garcia-Gon NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling Sci Rep, 2019-09-25;9(1):13896. 2019-09-25 [PMID: 31554934] (ICC, Western Blot, Mouse)
• Francesca Bufalieri, Paola Infante, Flavia Bernardi, Miriam Caimano, Paolo Romania, Marta Moretti et al. ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of beta TrCP Nature Communications 2019-07-24 [PMID: 31341163]
• Heejin Nam, Shin Jeon, Hyejin An, Jaeyoung Yoo, Hyo-Jong Lee, Soo-Kyung Lee et al. Critical roles of ARHGAP36 as a signal transduction mediator of Shh pathway in lateral motor columnar specification eLife 2019-07-15 [PMID: 31305241]
• P Ma, NN Song, Y Li, Q Zhang, L Zhang, L Zhang, Q Kong, L Ma, X Yang, B Ren, C Li, X Zhao, Y Li, Y Xu, X Gao, YQ Ding, B Mao Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning Cell Rep, 2019-07-09;28(2):541-553.e4. 2019-07-09 [PMID: 31291587] (Western Blot, Human, Mouse)
• Yuhong Han, Bing Wang, Yong Suk Cho, Jian Zhu, Jiang Wu, Yongbin Chen et al. Phosphorylation of Ci/Gli by Fused Family Kinases Promotes Hedgehog Signaling Developmental Cell 2019-09-01 [PMID: 31279575]
• Christina R Adams, Htet Htwe Htwe, Timothy Marsh, Aprilgate L Wang, Megan L Montoya, Lakshmipriya Subbaraj et al. Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer eLife 2019-05-28 [PMID: 31134896]
• Anna A. Kiseleva, Vladislav A. Korobeynikov, Anna S. Nikonova, Peishan Zhang, Petr Makhov, Alexander Y. Deneka et al. Unexpected Activities in Regulating Ciliation Contribute to Off-target Effects of Targeted Drugs Clinical Cancer Research 2019-07-01 [PMID: 30867219]
• K He, X Ma, T Xu, Y Li, A Hodge, Q Zhang, J Torline, Y Huang, J Zhao, K Ling, J Hu Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules Nat Commun, 2018-08-17;9(1):3310. 2018-08-17 [PMID: 30120249] (ICC, Human)
• Kerstin Hasenpusch-Theil, Stephen West, Alexandra Kelman, Zrinko Kozic, Sophie Horrocks, Andrew P. McMahon et al. Gli3 controls the onset of cortical neurogenesis by regulating the radial glial cell cycle through Cdk6 expression Development 2018-08-20 [PMID: 30093555]
• SR Hong, CL Wang, YS Huang, YC Chang, YC Chang, GV Pusapati, CY Lin, N Hsu, HC Cheng, YC Chiang, WE Huang, NC Shaner, R Rohatgi, T Inoue, YC Lin Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling Nat Commun, 2018-04-30;9(1):1732. 2018-04-30 [PMID: 29712905] (ICC, Mouse)
• P Infante, R Faedda, F Bernardi, F Bufalieri, L Lospinoso, R Alfonsi, D Mazzà, M Siler, S Coni, A Po, M Petroni, E Ferretti, M Mori, E De Smaele, G Canettieri, C Capalbo, M Maroder, I Screpanti, M Kool, SM Pfister, D Guardavacc, A Gulino, L Di Marcotu Itch/?-arrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signalling and medulloblastoma tumorigenesis Nat Commun, 2018-03-07;9(1):976. 3/7/2018 [PMID: 29515120]
• T Rosengren, LJ Larsen, LB Pedersen, ST Christense, LB Møller TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms Cell. Mol. Life Sci., 2018-02-02;0(0):. 2018-02-02 [PMID: 29396625] (Western Blot, Mouse)
• A Struchtrup, A Wiegering, B Stork, U Rüther, C Gerhardt The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts Autophagy, 2018-02-21;0(0):1-47. 2018-02-21 [PMID: 29372668] (Western Blot, Mouse)
• LK Veistinen, T Mustonen, MR Hasan, M Takatalo, Y Kobayashi, DA Kesper, A Vortkamp, DP Rice Regulation of Calvarial Osteogenesis by Concomitant De-repression of GLI3 and Activation of IHH Targets Front Physiol, 2017-12-19;8(0):1036. 2017-12-19 [PMID: 29311969] (IHC, Western Blot, Mouse)
• Ganesh V. Pusapati, Jennifer H. Kong, Bhaven B. Patel, Arunkumar Krishnan, Andreas Sagner, Maia Kinnebrew et al. CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog Developmental Cell 2018-01-08 [PMID: 29290584]
• Yeon‐Joo Kim, Daniel PS Osborn, Ji‐Young Lee, Masatake Araki, Kimi Araki, Timothy Mohun et al. WDR11‐mediated Hedgehog signalling defects underlie a new ciliopathy related to Kallmann syndrome EMBO reports 2018-02-01 [PMID: 29263200] (Western Blot, Human, Mouse, Transgenic Mouse)
• VJ Guen, TE Chavarria, C Kröger, X Ye, RA Weinberg, JA Lees EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling Proc. Natl. Acad. Sci. U.S.A., 2017-11-20;0(0):. 2017-11-20 [PMID: 29158396] (IHC-P, Mouse)
• D Hasegawa, H Ochiai-Shi, S Onodera, T Nakamura, A Saito, T Onda, K Watanabe, K Nishimura, M Ohtaka, M Nakanishi, K Kosaki, A Yamaguchi, T Shibahara, T Azuma Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction PLoS ONE, 2017-10-31;12(10):e0186879. 2017-10-31 [PMID: 29088246] (Western Blot, Human)
• Naoki Nakagawa, Jingjun Li, Keiko Yabuno-Nakagawa, Tae-Yeon Eom, Martis Cowles, Tavien Mapp et al. APC sets the Wnt tone necessary for cerebral cortical progenitor development Genes & Development 2017-08-15 [PMID: 28916710]
• J. Aaron Crapster, Louanne Hudgins, James K. Chen, Natalia Gomez-Ospina A novel missense variant in the GLI3 zinc finger domain in a family with digital anomalies American Journal of Medical Genetics Part A 2017-12-01 [PMID: 28884880]
• Gonnissen A, Isebaert S, Perneel C et al. Tissue microarray analysis indicates hedgehog signaling as a potential prognostic factor in intermediate-risk prostate cancer BMC Cancer 9/6/2017 [PMID: 28877722]
• J Li, Y Cui, J Xu, Q Wang, X Yang, Y Li, X Zhang, M Qiu, Z Zhang, Z Zhang Suppressor of Fused Restraint of Hedgehog Activity Level is Critical for Osteogenic Proliferation and Differentiation during Calvarial Bone Development J. Biol. Chem., 2017-08-09;0(0):. 2017-08-09 [PMID: 28794157] (Western Blot, Mouse)
• Y Han, Y Xiong, X Shi, J Wu, Y Zhao, J Jiang Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-?2 nuclear import system PLoS Biol., 2017-08-04;15(8):e2002063. 2017-08-04 [PMID: 28777795] (Western Blot, Mouse)
• Maria A. Hoelzl, Karin Heby-Henricson, Marco Gerling, José M. Dias, Raoul V. Kuiper, Cornelius Trünkle et al. Differential requirement of SUFU in tissue development discovered in a hypomorphic mouse model Developmental Biology 2017-09-01 [PMID: 28688895]
• Nadia B. Hassounah, Martha Nunez, Colleen Fordyce, Denise Roe, Ray Nagle, Thomas Bunch et al. Inhibition of Ciliogenesis Promotes Hedgehog Signaling, Tumorigenesis, and Metastasis in Breast Cancer Molecular Cancer Research 2017-10-01 [PMID: 28611083] (Western Blot, Transgenic Mouse)
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• Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling Proc. Natl. Acad. Sci. U.S.A, 2016-12-05;0(0):. 2016-12-05 [PMID: 27930311] (Western Blot, Human)
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• Mechanism of inhibition of the tumor suppressor Patched by Sonic Hedgehog Proc Natl Acad Sci USA, 2016-09-19;0(0):. 2016-09-19 [PMID: 27647915] (Western Blot, Mouse)
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Reviews for GLI-3 Antibody (AF3690) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 11/13/2015

Summary

• Application: Western Blot
• Sample Tested: NIH-3T3 cells
• Species: Mouse
• Lot: YLD0109011

reviewed by: Verified Customer WB Mouse 01/08/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22173325
• Species: Mouse

reviewed by: Verified Customer WB Mouse 01/08/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22547067
• Species: Mouse

Bioinformatics

• Gene Symbol: GLI3
• Uniprot:
  • P10071()