Mouse GLP1 ELISA Kit (Colorimetric)

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ELISA: Mouse GLP1 ELISA Kit (Colorimetric) [NBP3-18782] - Samples were spiked with high concentrations of Mouse GLP1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16-10 ng/mL
Sensitivity
0.1 ng/mL
Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

Mouse GLP1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse GLP1 in samples. No significant cross-reactivity or interference between Mouse GLP1 and analogues was observed.
Standard Curve Range
0.16-10 ng/mL
Sensitivity
0.1 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 5.04
Intra-Assay
%CV < 6.00
Spike Recovery
87-109%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GCG

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse GLP1 ELISA Kit (Colorimetric)

  • glicentin-related polypeptide
  • GLP1
  • GLP-1
  • glucagon-like peptide 1
  • GRPP

Background

Glucagon-like peptide-1 (GLP-1) is an incretin hormone secreted from enteroendocrine L cells in response to ingested nutrients. The closely related peptides glucagon-like peptide (GLP-1) and glucagon have opposing effects on blood glucose. GLP-1 induces glucose-dependent insulin secretion in the pancreas, while glucagon stimulates gluconeogenesis and glycogenolysis in the liver. Glucagon is processed from a large precursor, proglucagon, in a tissue-specific manner in pancreatic alpha-cells. The identification of a hybrid peptide acting as both a GLP-1 agonist and a glucagon antagonist would provide a novel approach for the treatment of type 2 diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol GCG