Glucagon Antibody (5F1)

Western Blot: Glucagon Antibody (5F1) [NBP2-75507] - Analysis of Glucagon on mouse kidney tissue lysate using anti-Glucagon antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Glucagon Antibody (5F1) [NBP2-75507] - Staining Glucagon (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Glucagon Antibody (5F1) [NBP2-75507] - Analysis of paraffin-embedded rat pancreas tissue using anti-Glucagon antibody. Counter stained with hematoxylin.

Flow Cytometry: Glucagon Antibody (5F1) [NBP2-75507] - Analysis of PANC-1 cells with Glucagon antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunocytochemistry/Immunofluorescence: Glucagon Antibody (5F1) [NBP2-75507] - Staining Glucagon (green) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Glucagon Antibody (5F1) [NBP2-75507] - Staining Glucagon (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Glucagon Antibody (5F1) [NBP2-75507] - Analysis of paraffin-embedded mouse pancreas tissue using anti-Glucagon antibody. Counter stained with hematoxylin.

Product Discontinued

Glucagon Antibody (5F1) Summary

• Immunogen: Recombinant protein (SwissProt: P01275 Human; SwissProt: P55095 Mouse; SwissProt: P06883 Rat)
• Localization: Secreted.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: GCG
• Purity: Protein L purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein L purified

Alternate Names for Glucagon Antibody (5F1)

• GCG
• glicentin-related polypeptide
• GLP1
• GLP-1
• Glucagon
• glucagon-like peptide 1
• glucagon-like peptide 2
• GRPP

Background

The protein encoded by the Glucagon gene is actually a preproprotein that is cleaved into four distinct mature peptides. One ofthese, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulatingglycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signallingpathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promotenutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an activeenteroglucagon. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GCG