Glucagon Antibody (K79bB10)

Immunohistochemistry: Glucagon Antibody (K79bB10) [NB600-1506] - Mouse pancreatic islet alpha cells were stained using Monoclonal Anti-Glucagon, clone no. K79bB10, Anti-Mouse IgG-peroxidase and AEC substrate (red). The tissue was counterstained with hematoxylin (blue).

Immunohistochemistry: Glucagon Antibody (K79bB10) [NB600-1506] - Mouse pancreatic islet alpha cells were stained using Monoclonal Anti-Glucagon, clone no. K79bB10, Anti-Mouse IgG-peroxidase and AEC substrate (red). The tissue was counterstained with hematoxylin (blue).

• Reactivity: Hu, Mu, Rt, Po, Ca, Fe, Gp, Rb
• Applications: DB, Func, IHC
• Clone: K79bB10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Glucagon Antibody (K79bB10) Summary

• Immunogen: Polymerized porcine glucagon.
• Localization: Secreted
• Specificity: The antibody reacts specifically against pancreatic glucagon and exhibits only very weak cross-reaction with gut glucagon (enteroglucagon).Glucagon (K79bB10)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GCG
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:2000
  • Radioimmunoassay 1:100-1:2000
• Application Notes: Reacts with pancreatic glucagon. RIA: Use at an assay dependent dilution, affinity constant of 6.1 x 10^8 L/M, reacts with pancreatic glucagon.

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for Glucagon Antibody (K79bB10)

• GCG
• glicentin-related polypeptide
• GLP1
• GLP-1
• Glucagon
• glucagon-like peptide 1
• glucagon-like peptide 2
• GRPP

Background

The protein encoded by the Glucagon gene is actually a preproprotein that is cleaved into four distinct mature peptides. One ofthese, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulatingglycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signallingpathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promotenutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an activeenteroglucagon. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glucagon Antibody (NB600-1506)(5)

Publications using NB600-1506

• Andreas Frøslev Mathisen, Andrei Mircea Vacaru, Lucas Unger, Elena Mirela Lamba, Oana-Ana-Maria Mardare, Laura Maria Daian, Luiza Ghila, Ana-Maria Vacaru, Simona Chera Molecular profiling of NOD mouse islets reveals a novel regulator of insulitis onset Scientific Reports 2024-06-25 [PMID: 38918575]
• Herat LY, Matthews JR, Hibbs M et al. SGLT1/2 inhibition improves glycemic control and multi-organ protection in type 1 diabetes iScience 2023-08-18 [PMID: 37520739]
• GUndUz D, Cetin H, DOnmez AC Investigation of the effects of swimming exercises in rats given acrylamide Morphologie : bulletin de l'Association des anatomistes 2022-12-05 [PMID: 36481219]
• Lu TT, Heyne S, Dror E et al. The Polycomb-Dependent Epigenome Controls b Cell Dysfunction, Dedifferentiation, and Diabetes Cell Metab. 2018-06-05 [PMID: 29754954] (IF/IHC, Mouse)
• Zehetner J, Danzer C, Collins S et al. pVHL is a regulator of glucose metabolism insulin secretion in pancreatic {beta} cells. Genes amp;amp; Dev;22(22):3135-3146. 2008-01-01 [PMID: 19056893]

FAQs for Glucagon Antibody (NB600-1506). (Showing 1 - 1 of 1 FAQs).

1. Would you be able to confirm that product NB600-1506 is raised against polymerized glucagon w/ the sequence (HSQGTFTSDYSKYLDSRRAQDFVQWLMNT)?  ..show answer..
   • Product NB600-1506 was raised against the full length porcine polymerized glucagon sequence. That sequence shares 100% homology with the sequence you have provided in your message.

Bioinformatics

• Gene Symbol: GCG
• Entrez:
  • 2641(Human)
  • 14652(Mouse)
  • 24952(Rat)