GluR1 Antibody (S355-1) - BSA Free

Western Blot: GluR1 Antibody (S355-1) [NBP2-22399] - Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa GluR1 protein using Mouse Anti-GluR1 Monoclonal Antibody, Clone S355-1 (NBP2-22399). Load: 10 ug. Block: 5% milk + TBST. Primary Antibody: Mouse Anti-GluR1 Monoclonal Antibody (NBP2-22399) at 1:2000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.

Immunocytochemistry/Immunofluorescence: GluR1 Antibody (S355-1) [NBP2-22399] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluA1/GluR1 Monoclonal Antibody, Clone S355-1 (NBP2-22399). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluA1/GluR1 Monoclonal Antibody (NBP2-22399) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluA1/GluR1 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: GluR1 Antibody (S355-1) [NBP2-22399] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluA1/GluR1 Glutamate Receptor Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction. Magnification: 60X.

Immunocytochemistry/Immunofluorescence: GluR1 Antibody (S355-1) [NBP2-22399] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluA1/GluR1 Glutamate Receptor Monoclonal Antibody, Clone S355-1 (NBP2-22399). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluA1/GluR1 Glutamate Receptor Monoclonal Antibody (NBP2-22399) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GluA1/GluR1 Glutamate Receptor Antibody. (D) Composite.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S355-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GluR1 Antibody (S355-1) - BSA Free Summary

• Immunogen: Fusion protein amino acids 1-389 (extracellular N-terminus) of rat GluA1/GluR1
• Localization: Cell Membrane , Endoplasmic Reticulum , Cell Junction
• Specificity: Detects approx. 100kDa. Does not cross-react with GluR2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRIA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of GluA1/GluR1 Glutamate Receptor Antibody was sufficient for detection of GluA1/GluR1 in 20 ug of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for GluR1 Antibody (S355-1) - BSA Free

• AMPA 1
• AMPA-selective glutamate receptor 1
• GluA1
• GLUH1
• GluR1
• gluR-1
• GLUR1gluR-A
• GLURA
• GluR-A
• gluR-K1
• glutamate receptor 1
• Glutamate receptor ionotropic, AMPA 1
• glutamate receptor, ionotropic, AMPA 1
• GRIA1
• HBGR1
• MGC133252

Background

Glutamate Receptor 1 belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for Glutamate Receptor 1. Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GluR1 Antibody (NBP2-22399)(3)

Publications using NBP2-22399

• Phadke R, Kruzich E, Fournier L et al. C4 induces pathological synaptic loss by impairing AMPAR trafficking bioRxiv 2023-09-11 (IHC-P, Mouse)
  
  Details:
  1:250 IHC-P dilution
• Kruzich E, Phadke RA, Brack A et al. A pipeline for STED super-resolution imaging and Imaris analysis of nanoscale synapse organization in mouse cortical brain slices STAR protocols 2023-11-08 [PMID: 37948187] (ICC/IF, Human)
  
  Details:
  Dilution 1:250
• Guo R, Zhao Y, Zhang M et al. Down-regulation of Stargazin inhibits the enhanced surface delivery of alpha -amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor GluR1 subunit in rat dorsal horn and ameliorates postoperative pain. Anesthesiology 2014-09-12 [PMID: 25093662] (IF/IHC, Rat)

Bioinformatics

• Gene Symbol: GRIA1
• Uniprot:
  • P19490(Rat)