NMDAR2B Antibody - C-terminus - Azide Free

Western Blot: NMDAR2B Antibody [NB300-106] - The blots show the examples of immunobands against NMDAR2B (C) and beta-actin antibodies on the membrane, which was performed on the membrane protein prepared from the lumbar spinal cords of the offspring (P14) rats in different groups. Image collected and cropped by CiteAb from the following publication (//www.jbiomedsci.com/content/18/1/64) licensed under a CC-BY license.

Flow Cytometry: NMDAR2B Antibody [NB300-106] - NMDA2B receptor expression on CD4+ T cells. Image supplied by review from confirmed customer.

Western Blot: NMDAR2B Antibody [NB300-106]

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

NMDAR2B Antibody - C-terminus - Azide Free Summary

• Description: PLEASE NOTE: If 0.01mg size is ordered, product will come lyophilized from 5 mM ammonium bicarbonate. Please reconstitute lyophilized product in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4)
• Immunogen: Fusion protein from the C-terminal region of the NR2B subunit. Accession # Q00960
• Localization: NB300-106 can be used to demonstrate that NR2B is highly enriched in hippocampus compared to either cortex or cerebellum.
• Specificity: Specific for the ~180kDa NR2B subunit of the NMDA receptor. Recognizes human, mouse and rat forms of the NR2B subunits of NMDAR. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody. No reactivity towards the NR2A and NR2C subunits.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRIN2B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:1000-1:2000
  • Immunoprecipitation 3 ul per 200 ug lysate
  • Western Blot 1:1000
• Application Notes: NB300-106 can be used in Western blot where a band is seen at ~ 180 kDa representing the NMDAR2B. Immunohistochemical analysis can be performed using frozen or fixed tissue sections. NB300-106 can also be used to quantitatively immunoprecipitate NMDAR2B where 3 ul will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2B in 200 mg of rat brain. NB300-106 has also been used successfully in immunofluoresence as reported in the literature (PMID: 20082293) and reported by an anonymous customer review. After reconstitution the antibody should be aliquoted and stored at -20C. Adequate amount of material to conduct 10-mini Western Blots.
• Theoretical MW: 180 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for NMDAR2B Antibody - C-terminus - Azide Free

• glutamate [NMDA] receptor subunit epsilon-2
• glutamate receptor subunit epsilon-2
• glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• hNR3
• MGC142180
• NMDAR2BMGC142178
• N-methyl D-aspartate receptor subtype 2B
• N-methyl-D-aspartate receptor subunit 3
• NR2B
• NR3

Background

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMDAR2B Antibody (NB300-106)(17)

Publications using NB300-106

• Yu XJ, Xiao T, Liu XJ et al. Effects of Nrf1 in Hypothalamic Paraventricular Nucleus on Regulating the Blood Pressure During Hypertension Frontiers in neuroscience 2021-12-06 [PMID: 34938159] (WB, Rat)
• Ahmed Eltokhi, Miguel A. Gonzalez-Lozano, Lars-Lennart Oettl, Andrey Rozov, Claudia Pitzer, Ralph Röth, Simone Berkel, Markus Hüser, Aliona Harten, Wolfgang Kelsch, August B. Smit, Gudrun A. Rappold, Rolf Sprengel Imbalanced post- and extrasynaptic SHANK2A functions during development affect social behavior in SHANK2-mediated neuropsychiatric disorders Molecular Psychiatry 2021-05-21 [PMID: 34021263]
• Hassani Nia, F, Woike, D Et al. Targeting of delta-catenin to postsynaptic sites through interaction with the Shank3 N-terminus. Mol Autism 2020-10-28 [PMID: 33115499] (IF/IHC, Mouse)
• Guadagno A, Verlezza S, Long H et al. It is all in the right amygdala: increased synaptic plasticity and perineuronal nets in male, but not female juvenile rat pups after exposure to early-life stress J. Neurosci. 2020-09-25 [PMID: 32978287]
• Schluter A, Aksan B, Diem R et al. VEGFD Protects Retinal Ganglion Cells and, consequently, Capillaries against Excitotoxic Injury Molecular Therapy - Methods & Clinical Development 2020-06-01 [PMID: 32055648] (WB, Human, Mouse)
• TrovO L, Fuchs C, De Rosa R et al. The green tea polyphenol epigallocatechin-3-gallate (EGCG) restores CDKL5-dependent synaptic defects in vitro and in vivo Neurobiol. Dis. 2020-02-04 [PMID: 32032735] (WB, Mouse)
• Kermath BA, Riha PD, Sajjad A, Gore AC. Effects of Chronic NMDA-NR2b Inhibition in the Median Eminence of the Reproductive Senescent Female Rat. J Neuroendocrinol. 2013-10-01 [PMID: 23957788] (ICC/IF, IF/IHC, Rat)
• Tse YC, Bagot RC, Hutter JA et al. Modulation of Synaptic Plasticity by Stress Hormone Associates with Plastic Alteration of Synaptic NMDA Receptor in the Adult Hippocampus. PLoS ONE 20116(11). [PMID: 22069501] (WB, Rat)
• Tao P-L, Chen C-F, Huang EY-K. Dextromethorphan attenuated the higher vulnerability to inflammatory thermal hyperalgesia caused by prenatal morphine exposure in rat offspring. J Biomed Sci;18(1):64. 2011-08-23 [PMID: 21861871] (WB, Rat)
• Snyder MA, Cooke BM, Woolley CS. Estradiol potentiation of NR2B-dependent EPSCs is not due to changes in NR2B protein expression or phosphorylation Hippocampus 2011-04-01 [PMID: 20082293] (WB, ICC/IF, IF/IHC, Rat)
• Maffucci JA, Noel ML, Gillette R et al. Age- and hormone-regulation of N-methyl-D-aspartate receptor subunit NR2b in the anteroventral periventricular nucleus of the female rat: implications for reproductive senescence. J Neuroendocrinol 2009-05-01 [PMID: 19302193] (IF/IHC, ICC/IF, Rat)
• Li L, El-Hayek YH, Liu B et al. Direct-current Electrical Field Guides Neuronal Stem/progenitor Cell Migration. Stem Cells : 2007-1022. 2008-01-01 [PMID: 18556511] (IP, ICC/IF, Rat)
• Park CS, Elgersma Y, Grant SG et al. alpha-Isoform of calcium-calmodulin-dependent protein kinase II and postsynaptic density protein 95 differentially regulate synaptic expression of NR2A- and NR2B-containing N-methyl-d-aspartate receptors in hippocampus. Neuroscience 2008-01-02 [PMID: 18082335] (WB, Rat)
• Head BP, Patel HH, Tsutsumi YM et al. Caveolin-1 expression is essential for N-methyl-D-aspartate receptor-mediated Src and extracellular signal-regulated kinase 1/2 activation and protection of primary neurons from ischemic cell death. FASEB J. 2008-03-01 [PMID: 17905724] (ICC/IF, Mouse)
  
  Details:
  IF/ICC: Cultured mouse cortical neurons, Fig 2.
• Gabra, Bichoy H et al. Decrease in N-Methyl-D-aspartic Acid Receptor-NR2B Subunit Levels by Intrathecal Short-Hairpin RNA Blocks Group I Metabotropic Glutamate Receptor-Mediated Hyperalgesia. J Pharmacol. Exp. Ther. 322: 186-194. 2007-01-01 [PMID: 17405869] (WB, Mouse)
• Ning, K et al. Dual Neuroprotective Signaling Mediated by Downregulating Two Distinct Phosphatase Activities of PTEN. The Journal of Neuroscience; 24: 4052-4060. 2004-01-01 [PMID: 15102920] (IP, WB, Rat)
• Gore, A et al. Gonadotropin-releasing hormone neurons, NMDA receptors, their regulation by steroid hormines across the reproductive life cycle. Brain Research Reviews 37:235-248. 2001-01-01 [PMID: 11744089]

Reviews for NMDAR2B Antibody (NB300-106) (3)

Average Rating: 4

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Mouse, Other.

reviewed by: Verified Customer Flow 01/31/2014

Summary

• Application: Flow Cytometry
• Lot: CS212R

reviewed by: Samantha Garcia WB Mouse 11/06/2013

Summary

• Application: Western Blot
• Sample Tested: Mouse brain synaptosomal membrane
• Species: Mouse
• Lot: cvp110

reviewed by: Verified Customer ICC Other 03/23/2010

Summary

• Application: Immunocytochemistry
• Sample Tested: Rat brain tissue
• Species: Other
• Lot: cvp608

FAQs for NMDAR2B Antibody (NB300-106). (Showing 1 - 1 of 1 FAQs).

1. Because I'm looking at staining in a region notorious for high background, they want me to do this control. Could you send me the sequence so that I can have the peptide synthesized?
   • Unfortunately the sequence for the fusion protein is proprietary.

Bioinformatics

• Gene Symbol: GRIN2B
• Entrez:
  • 2904(Human)
  • 14812(Mouse)
  • 24410(Rat)
• Uniprot:
  • Q00960(Rat)