GMDS Antibody - BSA Free

Western Blot: GMDS Antibody [NBP1-33424] - a GMDS mRNA level in A549 cells and H1299 cells infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA examined by quantitative real-time PCR (normalized to GAPDH mRNA). Data shown here was one out of three independent experiments (**p < 0.01). b Relative GMDS protein level in A549 cells and H1299 cells infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA examined by western blot. GAPDH protein was used as internal control. c-d. Representative microscope pictures of A549 cells. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4524-1) licensed under a CC-BY license.

Immunohistochemistry: GMDS Antibody [NBP1-33424] - Representative images of GMDS IHC staining in human lung adenocarcinoma and adjacent normal tissues. (Magnification, left is 20x, right is 100x). Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4524-1) licensed under a CC-BY license.

Western Blot: GMDS Antibody [NBP1-33424] - GMDS protein level in different cell lines including BEAS-2B (1), MRC-5 (2), HEK-293 (3), A549 (4), H1299 (5), SPC-A-1 (6). A59, H1299 and SPC-A-1 are all human lung adenocarcinoma cell lines. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4524-1) licensed under a CC-BY license.

Western Blot: GMDS Antibody [NBP1-33424] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 10% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: GMDS Antibody [NBP1-33424] - DLD1 xenograft, using GMDS antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: GMDS Antibody [NBP1-33424] - Human breast carcinoma. GMDS antibody [N2C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: GMDS Antibody [NBP1-33424] - Impaired cell proliferation in human lung adenocarcinoma cell lines with GMDS knockdown via Cellomics ArrayScan VTI. a GMDS mRNA level in A549 cells & H1299 cells infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA examined by quantitative real-time PCR (normalized to GAPDH mRNA). Data shown here was one out of three independent experiments (**, p < 0.01). b Relative GMDS protein level in A549 cells & H1299 cells infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA examined by western blot. GAPDH protein was used as internal control. c-d. Representative microscope pictures of A549 cells (c) & H1299 cells (d) infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA at different time points. e-f. Proliferation profiling of A549 cells (e) & H1299 cells (f) infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA for continuous 5 days examined by Cellomics ArrayScan VTI. Histogram shown here was relative fold changes of cell numbers compared to Day 1 & representing the mean ± SEM of three independent experiments (**, p < 0.01) Image collected & cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4524-1), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Bv, RM, Xp
• Applications: WB, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GMDS Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human GMDS. The exact sequence is proprietary.
• Predicted Species: Mouse (99%), Rat (98%), Xenopus (93%), Rhesus Macaque (99%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GMDS
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (93%), Zebrafish (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for GMDS Antibody - BSA Free

• EC 4.2.1.47
• GDP-D-mannose dehydratase
• GDP-mannose 4,6-dehydratase
• GMDGDP-mannose 4,6 dehydratase
• SDR3E1
• short chain dehydrogenase/reductase family 3E, member 1

Background

GDP-mannose 4,6-dehydratase (GMD; EC 4.2.1.47) catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose,the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor. The second and third stepsof the pathway are catalyzed by a single enzyme, GDP-keto-6-deoxymannose 3,5-epimerase, 4-reductase, designated FX inhumans (MIM 137020).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

&#9888; WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for GMDS Antibody (NBP1-33424)(1)

Publication using NBP1-33424

• Wei X, Zhang K, Qin H et al. GMDS knockdown impairs cell proliferation and survival in human lung adenocarcinoma BMC Cancer 2018-05-29 [PMID: 29843634] (WB, IHC-P, Human)

Bioinformatics

• Gene Symbol: GMDS
• Entrez:
  • 2762(Human)
• Uniprot:
  • O60547(Human)