GR/NR3C1 Antibody

Western Blot: GR/NR3C1 Antibody [NBP2-42221] - Wild-type (WT) and Glucocorticoid receptor knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid receptor antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: GR/NR3C1 Antibody [NBP2-42221] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Glucocorticoid Receptor antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: GR/NR3C1 Antibody [NBP2-42221] - Human lung cancer. Glucocorticoid Receptor stained by Glucocorticoid Receptor antibody (GR/NR3C1)

Immunohistochemistry-Paraffin: GR/NR3C1 Antibody [NBP2-42221] - Human colon carcinoma, using Glucocorticoid receptorantibody at 1:250 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunoprecipitation: GR/NR3C1 Antibody [NBP2-42221] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of Glucocorticoid Receptor beta protein by 2. 5 ug of Glucocorticoid Receptor beta antibody 7. 5% SDS-PAGE The immunoprecipitated Glucocorticoid Receptor beta protein was detected by Glucocorticoid Receptor beta antibody diluted at 1:1000

Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody [NBP2-42221] - GR/NR3C1 antibody detects GR/NR3C1 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GR/NR3C1 stained by GR/NR3C1 antibody (NBP2-42221) diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Fe
• Applications: WB, ICC/IF, IHC, IP, ChIP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GR/NR3C1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human GR/NR3C1. The exact sequence is proprietary.
• Localization: Cytoplasm; Nucleus
• Predicted Species: Feline (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR3C1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation Sequencing Reported in scientific literature (PMID:35077705)
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:500-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine reactivity reported in scientific literature (Ruiz-Conca M et al). Mouse (89%), Rat (87%), Sheep (88%), Canine (87%), Guniea Pig (87%), Porcine (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for GR/NR3C1 Antibody

• GCR
• glucocorticoid receptor
• GR
• GRGCCR
• GRLNuclear receptor subfamily 3 group C member 1
• NR3C1
• nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• nuclear receptor subfamily 3, group C, member 1

Background

The protein encoded by this gene is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for GR/NR3C1 Antibody (NBP2-42221)(5)

Publications using NBP2-42221

• Ruiz-Conca M, Gardela J, Alvarez-Rodriguez M et al. Immunofluorescence analysis of NR3C1 receptor following cortisol exposure during bovine in vitro oocyte maturation AETE Abstract 2019-09-12 (ICC/IF, Bovine)
• Hoffman JA, Trotter KW, Day CR et al. Multimodal regulatory elements within a hormone-specific super enhancer control a heterogeneous transcriptional response Molecular cell 2022-01-20 [PMID: 35077705] (Chip Cytometry, Human)
• Leung C, Kosyk O, Welter E et al. Naturalistic Glucocorticoid Receptor Activation Programs Key Cell Phenotypes and Functional Epigenomic Patterns in Human Fibroblasts SSRN Electronic Journal
• Leung C, Kosyk O, Welter E et al. Chronic stress-driven glucocorticoid receptor activation programs key cell phenotypes and functional epigenomic patterns in human fibroblasts iScience 2022-09-01 [PMID: 36065188] (Chemotaxis, Human)
• Ruiz-Conca M, Gardela J, Mogas T et al. Apoptosis and glucocorticoid-related genes mRNA expression is modulated by coenzyme Q10 supplementation during in vitro maturation and vitrification of bovine oocytes and cumulus cells Theriogenology 2022-10-01 [PMID: 36063671] (WB, Bovine)

Bioinformatics

• Gene Symbol: NR3C1
• Uniprot:
  • P04150()