Growth Hormone Antibody (GH-45) - BSA Free

ELISA: Growth Hormone Antibody (GH-45) [NB500-364] - ELISA of human growth hormone using mouse monoclonal antibody GH-45. Compared with mouse IgG1 isotype control antibody MOPC-21.

• Reactivity: Hu
• Applications: ELISA, ICC/IF, IHC
• Clone: GH-45
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Growth Hormone Antibody (GH-45) - BSA Free Summary

• Immunogen: Human growth hormone
• Specificity: The antibody GH-45 reacts with human growth hormone (hGH), a polypeptide hormone synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. The GH-45 antibody reacts with affinity constant 3.8 x 1010 l /mol; it does not bind human prolactin or any other pituitary hormones.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GH1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Paraffin 1:200
• Application Notes: ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant concentration. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
• Control:

  Growth Hormone Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for Growth Hormone Antibody (GH-45) - BSA Free

• GH1
• GHB5
• GHIGHD1B
• GHN
• GH-N
• GHNGrowth hormone
• GH-Nsomatotropin
• growth hormone 1Pituitary growth hormone
• Growth Hormone
• hGH-N
• IGHD1A
• IGHD2
• Somatotropin

Background

Human growth hormone (hGH) plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. The effects of hGH in the body can be generally described as anabolic. It stimulates amino acid uptake and protein synthesis in muscle and other tissues. Growth Hormone (GH1) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Genes for growth hormone (GH) are found in a gene cluster on 17q, which consists of two growth hormone genes and three CSH genes. The GHN1 gene is transcribed exclusively in the pituitary, whereas the other 4 genes are expressed only in placental tissues. Several isoforms of GH1 exist. Human growth hormone (hGH) is also used as a reporter protein, because it is secreted from mammalian cells it can be assayed by ELISA on culture supernatant using antibodies to determine the level of expression. Detection of growth hormone producing cells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Growth Hormone Antibody (NB500-364)(2)

Publications using NB500-364

• Park SH, Jang JH, Lee YM et al. Function of cell-cycle regulators in predicting silent pituitary adenoma progression following surgical resection Oncol Lett. [PMID: 29344143] (IF/IHC, Human)
• Thompson LD, Seethala RR, Muller S. Ectopic Sphenoid Sinus Pituitary Adenoma (ESSPA) with Normal Anterior Pituitary Gland: A Clinicopathologic and Immunophenotypic Study of 32 Cases with a Comprehensive Review of the English Literature Head Neck Pathol 4098-01-01 [PMID: 22430769] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: GH1
• Entrez:
  • 2688(Human)