Recombinant Human Growth Hormone Protein

Recombinant Human Growth Hormone Protein [NBC1-18450]

Product Discontinued

Recombinant Human Growth Hormone Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 27-217 of Human Growth Hormone
  
  

  Source: E.coli

  Amino Acid Sequence: MFPTIPLSRL FDNAMLRAHR LHQLAFDTYQ EFEEAYIPKE QKYSFLQNPQ TSLCFSESIP TPSNREETQQ KSNLELLRIS LLLIQSWLEP VQFLRSVFAN SLVYGASDSN VYDLLKDLEE GIQTLMGRLE DGSPRTGQIF KQTYSKFDTN SHNDDALLKN YGLLYCFRKD MDKVETFLRI VQCRSVEGSC GF

• Details of Functionality: Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 for this effect is less or equal to 0.2 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GH1
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 22.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 88 mM mannitol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Growth Hormone Protein

• GH1
• GHB5
• GHIGHD1B
• GHN
• GH-N
• GHNGrowth hormone
• GH-Nsomatotropin
• growth hormone 1Pituitary growth hormone
• Growth Hormone
• hGH-N
• IGHD1A
• IGHD2
• Somatotropin

Background

Human growth hormone(hGH, somatotropin), a protein hormone from the anterior lobe of the pituitary gland, comprises a single polypeptide chain of 191 amino acid residues. The growth hormone amino acid sequence varies considerably between species, and non-primate growth hormones have little activity in man. Its main function is the stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues. Recombinant human growth hormone(rhGH ) was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GH1