GSK-3 beta [p Tyr279, p Tyr216] Antibody - BSA Free

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, NB100-81946 was diluted at 1:200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.

Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Western blot analysis of GSK-3 beta [p Tyr279, p Tyr216] expression in mouse testis (A), rat testis (B), rat spleen (C) whole cell lysates.

Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Analysis of extracts of various cell lines, using NB100-81946

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81946(left) or the same antibody preincubated with blocking peptide (right).

Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Methanol-fixed MEF cells.

Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Staining of Methanol-fixed Hela cells.

• Reactivity: Hu, Mu, Rt, Ma
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GSK-3 beta [p Tyr279, p Tyr216] Antibody - BSA Free Summary

• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human GSK-3 beta around the phosphorylation site of tyrosine 279/216 (V-S-YP-I-C).
• Modification: p Tyr216, p Tyr279
• Specificity: Detects endogenous levels of GSK-3 beta only when phosphorylated at tyrosine 216/279.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GSK3B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:1000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  GSK-3 beta Overexpression Lysate

Reactivity Notes

Use in Mammal reported in scientific literature (PMID:33413691)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GSK-3 beta [p Tyr279, p Tyr216] Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.26
• glycogen synthase kinase 3 beta
• glycogen synthase kinase-3 beta
• GSK3 beta
• GSK-3 beta
• GSK3B
• GSK3beta isoform

Background

Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GSK-3 beta Antibody (NB100-81946)(11)

Publications using NB100-81946

• Takahiro Toshima, Mikako Yagi, Yura Do, Haruka Hirai, Yuya Kunisaki, Dongchon Kang, Takeshi Uchiumi Mitochondrial translation failure represses cholesterol gene expression via Pyk2-Gsk3 beta -Srebp2 axis Life Science Alliance 2024-05-08 [PMID: 38719751]
• Xu M, Lantz MJ, Nichols RA, Li QX Anti-Neuroinflammatory Effects of a Semi-Synthetic Isoorientin-Based Glycogen Synthase Kinase-3beta Inhibitor in Lipopolysaccharide-Activated Microglial Cells ACS chemical neuroscience 2022-01-05 [PMID: 34913695]
• Hsing C, Tsai C, Chen C Et Al. Pharmacologically Inhibiting Glycogen Synthase Kinase-3beta Ameliorates Renal Inflammation and Nephrotoxicity in an Animal Model of Cisplatin-Induced Acute Kidney Injury Biomedicines 2021-07-25 [PMID: 34440091] (IHC-P)
• Takaichi Y, Chambers JK, Ano Y et al. Deposition of Phosphorylated alpha -Synuclein and Activation of GSK-3 beta and PP2A in the PS19 Mouse Model of Tauopathy Journal of neuropathology and experimental neurology 2021-06-21 [PMID: 34151989]
• Takaichi Y, Chambers JK, Takahashi K, et al. Amyloid beta and tau pathology in brains of aged pinniped species (sea lion, seal, and walrus) Acta neuropathologica communications 2021-01-07 [PMID: 33413691] (IHC-P, Mammal)
• Wu L, Lin W, Liao Q et al. Calcium Channel Blocker Nifedipine Suppresses Colorectal Cancer Progression and Immune Escape by Preventing NFAT2 Nuclear Translocation Cell Rep 2020-10-27 [PMID: 33113363] (WB, Human)
• Liu Z, Selby CP, Yang Y et al. Circadian regulation of c-MYC in mice Proc. Natl. Acad. Sci. U.S.A. 2020-08-19 [PMID: 32817420] (WB, Mouse)
• Takaichi Y, Chambers JK, Inoue H et al. Phosphorylation and oligomerization of alpha-synuclein associated with GSK-3 beta activation in the rTg4510 mouse model of tauopathy Acta Neuropathol Commun 2020-06-19 [PMID: 32560668] (IF/IHC, Mouse)
• Wu X, Kosaraju J, Tam kY. Anti-neuroinflammatory effects of SLOH in AB-induced BV-2 microglial cells and 3xTg-AD mice involve the inhibition of GSk-3B. Neurosci. Lett. 2018-09-29 [PMID: 30278248] (WB, Mouse)
• Wu X, Kosaraju J, Zhou W, Tam KY. SLOH, a carbazole-based fluorophore, mitigates neuropathology and behavioral impairment in the triple-transgenic mouse model of Alzheimer's disease Neuropharmacology 2018-01-05 [PMID: 29309769] (Mouse)
• Denysenko T, Annovazzi L, Cassoni P et al. WNT/b-catenin Signaling Pathway and Downstream Modulators in Low- and High-grade Glioma. Cancer Genomics Proteomics. 2015-12-28 [PMID: 26708597] (IHC-P, Human)

Bioinformatics

• Gene Symbol: GSK3B
• Entrez:
  • 2932(Human)
  • 56637(Mouse)
  • 84027(Rat)
• Uniprot:
  • P49841()