HA Tag Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-363] - Detection of HA-tagged Tubulin in CHO cells transfected with mutant beta-tubulin cDNA encoding an HA epitope tag. Photo contributed by R. Bhattacharya and F. Cabral, University of Texas Medical School at Houston.

Western Blot: HA Tag Antibody [NB600-363] - 200, 100, or 50 ng of E. coli whole cell lysate expressing amulti-tag fusion protein.Antibody: Affinity purified rabbitanti-HA antibody used for WB at 0.04 ug/ml(1:25,000). Detection: Chemiluminescence with anexposure time of 3 seconds.

Western Blot: HA Tag Antibody [NB600-363] - Wnt/beta-catenin signaling regulates HySp5 expression. Immunoprecipitation (IP) of HA-tagged HySp5-420 expressed in HEK293T cells together or not with hudeltabeta-Catenin (upper) or huTCF1 (lower). IP was performed with an anti-HA antibody and Co-IP products were detected with the anti-beta-catenin or anti-TCF1 antibodies. Same results were obtained in two independent experiments. Each data point represents one biological independent experiment. Statistical p values: *p <= 0.05, **p <= 0.01, ***p <= 0.001, ****p <= 0.0001 (unpaired t test). Error bars indicate SD. PP primer pair, RLA relative luciferase activity Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-08242-2), licensed under a CC-BY license.

Western Blot: HA Tag Antibody [NB600-363] - ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors and pEGFP, cell lysates were probed with antibodies against the HA tag and EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, and 1200 ng. NT, non-transfected cells. Selection-independent generation of gene knockout mouse embryonic stem cells using zinc-finger nucleases. PLoS One (2011)

Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-363] - HA-tagged proteins were detected in immersion fixed HEK293 human embryonic kidney cell line transfected with HA-tagged MGAT2 using 1 ug/mL goat anti-HA Tag polyclonal (NB600-362, Novus Biologicals), 1 ug/mL rabbit anti-HA Tag polyclonal (NB600-363, Novus Biologicals). Cells were stained using the appropriate secondary antibody, donkey anti-mouse IgG-NL557 (NL007) and counterstained with DAPI (blue).

Western Blot: HA Tag Antibody [NB600-363] - Gene knockout in U2OS.693 cells.(A) Schematic of ZFN-mediated knockout. A ZFN pair (ZFNR & ZFNL) designed to target position 502 in the EGFP gene (E502) creates a DNA double-strand break that is sealed by the error-prone non-homologous end-joining (NHEJ) pathway & hence leads to disruption of the coding sequence. (B) Dose-dependent gene disruption in U2OS.693 cells. U2OS.693 cells that stably express a destabilized EGFP were transfected with increasing amounts of E502-specific ZFN expression vectors (75–1200 ng). The percentage of EGFP-negative cells was determined 6 days post-transfection by flow cytometry (n = 3; indicated is average & standard deviation). E502-WT, ZFN with wild-type FokI domain; E502-OH, ZFN with obligate heterodimeric FokI domain. (C) ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors & pEGFP, cell lysates were probed with antibodies against the HA tag & EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, & 1200 ng. NT, non-transfected cells. (D) Kinetics of EGFP knockout. The graph displays the percentage of EGFP-negative cells (see B) from day 1 to day 6 post-transfection for two vector amounts (n = 3; indicated is average & standard deviation). WT, E502-WT; OH, E502-OH. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22194948), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: ET
• Applications: WB, ELISA, Flow, GS, ICC/IF, IHC, IP, ChIP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.1 mg/ml

HA Tag Antibody - BSA Free Summary

• Immunogen: This HA Tag Antibody was developed by immunizing rabbits with HA cleavage site (YPYDVPDYA) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Gel Super Shift Assays
  • Immunocytochemistry/ Immunofluorescence 1:100-1:400
  • Immunohistochemistry 1:100-1:400
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Immunoprecipitation 1-4 ug/mg lysate
  • Knockout Validated
  • Western Blot 1:1000-1:10000
• Application Notes: Use in Gel Super Shift Assays reported in scientific literature (PMID:34289349). Use in ChIP reported in (PMID: 30659200).. Use in IHC reported in (PMID: 22880041).. Use in FLOW reported in scientific literature (Pryce R et al).. Knockout validation (PMID: 32354171).. Recommended dilution for coating ELISA plates is 1:100 - 1:500.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HA Tag Antibody - BSA Free

• HA Epitope Tag
• HA Tag
• HA
• Hemagglutinin

Background

Human influenza hemagglutinin (HA) is a glycoprotein required for the infectivity of the human virus and is expressed as a homotrimer on the surface of the viral capsid (1). As a major antigen of the influenza virus, HA constantly evolves to escape herd immunity via antigenic shift which can lead to pandemics (2). The HA tag (YPYDVPDYA-tag) itself has a molecular weight of 1.1 kDa and is a linear epitope derived from amino acids 98-106 of the HA protein. It is used extensively as a general epitope tag in expression vectors (2, 3). The HA tag is frequently added to the N- or C- terminus of a protein of interest to facilitate protein purification, detection, and labeling using anti-HA antibodies (3, 4). HA Tag is cleaved by caspase 3/7 resulting in total loss of immunoreactivity, making it unsuitable for the study of apoptosis (5).

References

1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076

2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778

3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093

4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73

5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HA Tag Antibody (NB600-363)(108)

Publications using NB600-363

• Mathews JA. ADAM10 exacerbation of allergic disease is potentially explained by its role in CD23 exosomal sorting. Virginia Commonwealth University. 187 pp. 2011-01-01
• Wells JR, Padua MB, Haaning AM et al. Non-coding cause of congenital heart defects: Abnormal RNA splicing with multiple isoforms as a mechanism for heterotaxy Human Genetics and Genomics Advances 2024-09-12 [PMID: 39275801]
• Trivedi P, Steele CD, Au FKC et al. Mitotic tethering enables inheritance of shattered micronuclear chromosomes Nature 2023-06-01 [PMID: 37316668]
• Sanger C The response of fibroblasts to hyperosmotic stress: Mechanisms and functions in skin homeostasis and wound healing Thesis 2023-01-01 (ICC/IF)
  
  Details:
  1:100 ICC/IF dilution
• Stevens CS Viruses Are the Best Cell Biologists: Viral Engineering to Deliver Gene Editing Tools and Interrogate SARS-CoV-2 Entry Thesis 2023-01-01
• Oguntuyo KY, Haas GD, Azarm KD et Al. Structure-guided mutagenesis of Henipavirus receptor-binding proteins reveals molecular determinants of receptor usage and antibody-binding epitopes J Virol 2024-03-01 [PMID: 38426726]
• Oguntuyo KY, Haas GD, Azarm KD et al. Structure guided mutagenesis of Henipavirus Receptor Binding Proteins reveals molecular determinants of receptor usage and antibody binding epitopes bioRxiv : the preprint server for biology 2023-11-22 [PMID: 38045373] (WB)
• Grasekamp KP, Beaud Benyahia B, Taib N et al. The Mla system of diderm Firmicute Veillonella parvula reveals an ancestral transenvelope bridge for phospholipid trafficking Nature communications 2023-11-23 [PMID: 37993432] (WB)
  
  Details:
  1:4000 dilution
• Wang, K;Papadopoulos, N;Hamidi, A;Lennartsson, J;Heldin, CH; SUMOylation of PDGF receptor ? affects signaling via PLC? and STAT3, and cell proliferation BMC molecular and cell biology 2023-05-16 [PMID: 37193980] (Epitope Tag)
• Chuong V, Farokhnia M, Khom S et Al. The glucagon-like peptide-1 (GLP-1) analogue semaglutide reduces alcohol drinking and modulates central GABA neurotransmission JCI Insight 2023-05-16 [PMID: 37192005]
• Seo-Yeon Jeong, Gurusamy Hariharasudhan, Min-Ji Kim, Ji-Yeon Lim, Sung Mi Jung, Eun-Ji Choi, In-Youb Chang, Younghoon Kee, Ho Jin You, Jung-Hee Lee SIAH2 regulates DNA end resection and replication fork recovery by promoting CtIP ubiquitination Nucleic Acids Research 2022-10-14 [PMID: 36155803]
• Wible D, Chao H, Tang D, Bratton S ATG5 cancer mutations and alternative mRNA splicing reveal a conjugation switch that regulates ATG12 ATG5-ATG16L1 complex assembly and autophagy Cell Discov Dec 1 2019 (WB, Human)
• Pryce R, Azarm K, Rissanen I et al. A key region of molecular specificity orchestrates unique ephrin-B1 utilization by Cedar virus bioRxiv (FLOW)
• Carusillo A, Haider S, Schäfer R et al. A novel Cas9 fusion protein promotes targeted genome editing with reduced mutational burden in primary human cells Nucleic acids research 2023-04-18 [PMID: 37070192] (ICC/IF, Human)
• Theivakadadcham VSS, Rosonina E Ethanol stress stimulates sumoylation of transcription factor Cst6 which restricts expression of its target genes bioRxiv 2019-09-05 (Chemotaxis, Yeast)
• Theivakadadcham VSS Regulation of Yeast Transcription Factors Sko1 and Cst6 by Sumoylation Thesis 2020-01-01 (Chemotaxis, Non-species specific)
• Ikegame S, Carmichael JC, Wells H et al. Metagenomics-enabled reverse-genetics assembly and characterization of myotis bat morbillivirus Nature microbiology 2023-06-01 [PMID: 37142773] (FLOW, WB, Epitope Tag)
  
  Details:
  Dilutions: Flow 1:1,000,WB: 1:1,000
• G Pizzolato, L Moparthi, S Söderholm, C Cantù, S Koch The oncogenic transcription factor FOXQ1 is a differential regulator of Wnt target genes Journal of Cell Science, 2022-10-10;0(0):. 2022-10-10 [PMID: 36124643]
• Peter AS, Gruner E, Socher E Et al. Characterization of SARS-CoV-2 Escape Mutants to a Pair of Neutralizing Antibodies Targeting the RBD and the NTD Int J Mol Sci 2022-07-28 [PMID: 35897753] (FLOW)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Oostindie SC, Rinaldi DA, Zom GG Et al. Logic-gated antibody pairs that selectively act on cells co-expressing two antigens Nat Biotechnol 2022-07-25 [PMID: 35879362] (SR Microscopy)
  
  Details:
  Citation using the Alexa Fluor 647 version of this antibody.
• Hallacli E, Kayatekin C, Nazeen S et al. The Parkinson's disease protein alpha-synuclein is a modulator of processing bodies and mRNA stability Cell 2022-06-09 [PMID: 35688132]
• Yang Y, Lu H, Chen C et al. HIF-1 Interacts with TRIM28 and DNA-PK to release paused RNA polymerase II and activate target gene transcription in response to hypoxia Nature communications 2022-01-14 [PMID: 35031618] (WB)
• Wang S, Liu H, Roberts JB et al. Prolonged ethanol exposure modulates constitutive internalization and recycling of 5-HT1A receptors Journal of neurochemistry 2021-12-20 [PMID: 34928513]
• Vitry J, Par� G, Murru A et al. Regulation of the Expression, Oligomerisation and Signaling of the Inhibitory Receptor CLEC12A by Cysteine Residues in the Stalk Region International Journal of Molecular Sciences 2021-09-22 [PMID: 34638548] (Western Blot)
• Lee B, Ikegame S, Carmichael J Et al. Zoonotic potential of a novel bat morbillivirus Research square 2021-09-29 [PMID: 34611656] (WB)
• Baig MS, Dou Y, Bergey BG Et al. Dynamic sumoylation of promoter-bound general transcription factors facilitates transcription by RNA polymerase II PLoS genetics 2021-09-01 [PMID: 34587155] (WB)
• Bugajev V, Paulenda T, Utekal P et al. Crosstalk between ORMDL3, serine palmitoyltransferase, and 5-lipoxygenase in the sphingolipid and eicosanoid metabolic pathways Journal of lipid research 2021-09-21 [PMID: 34560079] (IP)
• Cui Z, Dabas H, Leonard BC Et al. Caspase-8 mutations associated with head and neck cancer differentially retain functional properties related to TRAIL-induced apoptosis and cytokine induction Cell death & disease 2021-08-06 [PMID: 34362880] (WB)
• Tan K, Song HW, Wilkinson MF RHOX10 drives mouse spermatogonial stem cell establishment through a transcription factor signaling cascade Cell reports 2021-07-20 [PMID: 34289349] (GS)
• Par� G, Vitry J, Merchant ML et al. The Inhibitory Receptor CLEC12A Regulates PI3K-Akt Signaling to Inhibit Neutrophil Activation and Cytokine Release Frontiers in Immunology 2021-06-21 [PMID: 34234773] (Western Blot)
• Oguntuyo, K Y, Stevens, C S Et al. Quantifying Absolute Neutralization Titers against SARS-CoV-2 by a Standardized Virus Neutralization Assay Allows for Cross-Cohort Comparisons of COVID-19 Sera. MBio 2021-02-16 [PMID: 33593976] (ICC/IF)
• Malnar M, Rogelj B SFPQ regulates the accumulation of RNA foci and dipeptide repeat proteins from the expanded repeat mutation in C9orf72 Journal of cell science 2021-01-25 [PMID: 33495278] (ICC/IF, WB)
• Moparthi L, Koch S A uniform expression library for the exploration of FOX transcription factor biology Differentiation 2020-01-01 [PMID: 32858261] (IP, Non-species specific)
• Oguntuyo KY, Stevens CS, Hung CT et al. Quantifying absolute neutralization titers against SARS-CoV-2 by a standardized virus neutralization assay allows for cross-cohort comparisons of COVID-19 sera medRxiv 2020-08-15 [PMID: 32817961] (WB, Non-species specific)
• Ulman A, Skrzypek K, Konieczny P et al. Genome Editing of the SNAI1 Gene in Rhabdomyosarcoma: A Novel Model for Studies of Its Role Cells 2020-04-28 [PMID: 32354171] (Western Blot)
• Pino-Barrio MJ, GimEnez Y, Villanueva M et al. TALEN mediated gene editing in a mouse model of Fanconi anemia Sci Rep 2020-04-24 [PMID: 32332829] (WB)
• Kilisch M, Mayer S, Mitkovski M et al. A GTPase-induced switch in phospholipid affinity of collybistin contributes to synaptic gephyrin clustering J. Cell. Sci. 2020-01-23 [PMID: 31932505]
• Pryce R, Azarm K et al. A key region of molecular specificity orchestrates unique ephrin-B1 utilization by Cedar virus. Life Sci Alliance 2020-01-01 [PMID: 31862858] (FLOW, WB)
• Wible DJ, Chao HP, Tang DG et al. ATG5 cancer mutations and alternative mRNA splicing reveal a conjugation switch that regulates ATG12-ATG5-ATG16L1 complex assembly and autophagy Cell Discov 2019-08-27 [PMID: 31636955] (WB, Human)
• Zhang JR, Wang Y, Dong JX et al. Development of a Simple Pretreatment Immunoassay Based on an Organic Solvent-Tolerant Nanobody for the Detection of Carbofuran in Vegetable and Fruit Samples Biomolecules. 2019-10-07 [PMID: 31591300] (PAGE)
  
  Details:
  Citation used the HRP format of this antibody.
• Xiaoyu Tracy Cai, Hongjie Li, Abu Safyan, Jennifer Gawlik, George Pyrowolakis, Heinrich Jasper AWD regulates timed activation of BMP signaling in intestinal stem cells to maintain tissue homeostasis Nature Communications 2019-07-05 [PMID: 31278345]
• Sri Theivakadadcham VS, Bergey BG, Rosonina E. Sumoylation of DNA-bound transcription factor Sko1 prevents its association with nontarget promoters PLoS Genet. 2019-02-01 [PMID: 30763307] (Chemotaxis)
• Vogg MC, Beccari L, Iglesias Olle L et al. An evolutionarily-conserved Wnt3/b-catenin/Sp5 feedback loop restricts head organizer activity in Hydra. Nat Commun 2019-01-18 [PMID: 30659200] (Chemotaxis, WB, Human)
• Herring BP, Hoggatt AM, Gupta A, Wo JM. Gastroparesis is associated with decreased FOXF1 and FOXF2 in humans, and loss of FOXF1 and FOXF2 results in gastroparesis in mice Neurogastroenterol. Motil. 2018-12-19 [PMID: 30565344] (WB, Mouse)
• Lee D, Lee SA, Shin DM et al. Chloride Influx of Anion Exchanger 2 Was Modulated by Calcium-Dependent Spinophilin in Submandibular Glands Front Physiol 2018-07-19 [PMID: 30072910] (IP, Human)
• Xu M, Moresco JJ, Chang M et al. SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy PLoS Pathog. 2018-05-23 [PMID: 29791506] (WB, Human)
• Smith C. G, Kharkwal H, et al. Expression and Subcellular Localization of the Kaposi's Sarcoma-Associated Herpesvirus K15P Protein during Latency and Lytic Reactivation in Primary Effusion Lymphoma Cells. J Virol 2017-11-01 [PMID: 28835496] (ICC/IF, Rat)
• Murray AS, Varela FA, Hyland TE et al. Phosphorylation of the type II transmembrane serine protease, TMPRSS13 in Hepatocyte Growth Factor Activator Inhibitor-1 and 2-mediated cell surface localization J. Biol. Chem. 2017-07-14 [PMID: 28710277]
• Rissanen I, Ahmed AA, Azarm K et al. Idiosyncratic Mojiang virus attachment glycoprotein directs a host-cell entry pathway distinct from genetically related henipaviruses Nat Commun 2017-07-12 [PMID: 28699636] (WB, Human)
• Bednarski Christien, Tomczak Katja, Vom Hovel Beate et al. Targeted Integration of a Super-Exon into the CFTR Locus Leads to Functional Correction of a Cystic Fibrosis Cell Line Model. PLoS One 2016-01-01 [PMID: 27526025] (WB, Human)
• Coleman S, Choi KY, Root M, McGregor A. A Homolog Pentameric Complex Dictates Viral Epithelial Tropism, Pathogenicity and Congenital Infection Rate in Guinea Pig Cytomegalovirus PLoS Pathog. 2016-07-01 [PMID: 27387220] (IP)
• Park A, Yun T, Vigant F et al. Nipah Virus C Protein Recruits Tsg101 to Promote the Efficient Release of Virus in an ESCRT-Dependent Pathway. PLoS Pathog. 2016-05-01 [PMID: 27203423] (WB, IP, Human)
• Pentecost M, Vashisht AA, Lester T et al. Evidence for Ubiquitin-Regulated Nuclear and Subnuclear Trafficking among Paramyxovirinae Matrix Proteins. PLoS Pathog. 2015-03-01 [PMID: 25782006] (WB, Human)
• Sun S, Ling SC, Qiu J et al. ALS-causative mutations in FUS/TLS confer gain and loss of function by altered association with SMN and U1-snRNP. Nat Commun 2015-01-01 [PMID: 25625564] (WB, ICC/IF, Mouse, Human)
• Mussolino C, Alzubi J, Fine EJ et al. TALENs facilitate targeted genome editing in human cells with high specificity and low cytotoxicity. Nucleic Acids Res. 2014-05-03 [PMID: 24792154] (WB)
• Bobis-Wozowicz S, Galla M, Alzubi J et al. Non-integrating gamma-retroviral vectors as a versatile tool for transient zinc-finger nuclease delivery. Sci Rep 2014-04-11 [PMID: 24722320] (WB)
• Smith CJ, McGlade CJ. The ubiquitin ligase RNF126 regulates the retrograde sorting of the cation-independent mannose 6-phosphate receptor. Exp Cell Res. 2013-11-23 [PMID: 24275455] (ICC/IF, Human)
• Chen H, Kazemier HG, de Groote ML, Ruiters MH et al. Induced DNA demethylation by targeting Ten-Eleven Translocation 2 to the human ICAM-1 promoter. Nucleic Acids Res. 2013-11-04 [PMID: 24194590] (IP)
• Cowan J, Tariq M, Ware SM. Genetic and Functional Analyses of ZIC3 Variants in Congenital Heart Disease. Hum Mutat. 2013-10-07 [PMID: 24123890] (ICC/IF, Mouse)
• Horn AV, Klawitter S, Held U et al. Human LINE-1 restriction by APOBEC3C is deaminase independent and mediated by an ORF1p interaction that affects LINE reverse transcriptase activity. Nucleic Acids Res. 2013-10-07 [PMID: 24101588] (ICC/IF, Human)
• Smith CJ, Berry DM, McGlade CJ. The E3 ubiquitin ligases RNF126 and Rabring7 regulate endosomal sorting of the Epidermal Growth Factor Receptor. J Cell Sci 2013-02-15 [PMID: 23418353] (ICC/IF, Human)
• Hu YZ, Zhang J, Li S et al. The transcription activity of heat shock factor 4b is regulated by FGF2 Int J Biochem Cell Biol 2012-11-28 [PMID: 23200779] (ICC/IF, Chemotaxis)
• Rahman SH, Bobis-Wozowicz S, Chatterjee D et al. The non-toxic cell cycle modulator indirubin augments transduction of adeno-associated viral vectors and zinc-finger nuclease mediated gene targeting Hum Gene Ther 2012-10-16 [PMID: 23072634]
• Jung JJ, Tiwari A, Inamdar SM et al. Secretion of Soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR1/sFlt1) Requires Arf1, Arf6, and Rab11 GTPases PLoS One 2012-01-01 [PMID: 22962618] (WB)
• Di Gioia SA, Letteboer SJ, Kostic C et al. FAM161A, associated with retinitis pigmentosa, is a component of the cilia-basal body complex and interacts with proteins involved in ciliopathies. Hum Mol Genet 2012-12-01 [PMID: 22940612]
• Petridou NI, Stylianou P, Christodoulou N et al. Activation of Endogenous FAK via Expression of Its Amino Terminal Domain in Xenopus Embryos PLoS One 2012 [PMID: 22880041] (IHC, ICC/IF, WB, Xenopus)
• Yin S, Zeng C, Hari M et al. Random mutagenesis of beta-tubulin defines a set of dispersed mutations that confer paclitaxel resistance. Pharm Res 2012-11-01 [PMID: 22669706]
  
  Details:
  Using the FITC conjugated version of NB600-363, catalog number NB600-363F.
• Ishii J, Yoshimoto N, Tatematsu K et al. Cell wall trapping of autocrine peptides for human G-protein-coupled receptors on the yeast cell surface. PLoS One 2012-01-01 [PMID: 22623985]
• David M, Macias A, Moreno C et al. Protein Kinase C (PKC) Activity Regulates Functional Effects of Kv?1.3 Subunit on KV1.5 Channels: IDENTIFICATION OF A CARDIAC Kv1.5 CHANNELOSOME J Biol Chem 2012-06-15 [PMID: 22547057] (ICC/IF, Human)
• Zhao Y, Wang Y, Hu J, Zhang X, Zhang YW. CutA divalent cation tolerance homolog (E. coli) (CUTA) regulates Beta-cleavage of Beta-amyloid precursor protein (APP) through interacting with Beta-Site APP cleaving protein 1 (BACE1) J Biol Chem 2012-02-17 [PMID: 22351782] (WB, Human)
• Osiak A, Radecke F, Guhl E et al. Selection-independent generation of gene knockout mouse embryonic stem cells using zinc-finger nucleases. PLoS ONE 6(12). 2011-01-01 [PMID: 22194948]
• Schierling B, Dannemann N, Gabsalilow L et al. A novel zinc-finger nuclease platform with a sequence-specific cleavage module. Nucleic Acids Research. 2011-12-01 [PMID: 22135304]
• Liu YC, Lin MC, Chen HC, Tam MF, Lin LY. The role of small ubiquitin-like modifier-interacting motif in the assembly and regulation of metal-responsive transcription factor 1. J Biol Chem 2011-12-16 [PMID: 22021037] (WB, Chemotaxis)
• Shimizu Y, Sollu C, Meckler JF et al. Adding Fingers to an Engineered Zinc Finger Nuclease Can Reduce Activity. Biochemistry;50(22):5033-5041. 2011-06-07 [PMID: 21528840]
• Sollu C, Pars K, Cornu TI et al. Autonomous zinc-finger nuclease pairs for targeted chromosomal deletion. Nucleic Acids Res. 2010-08-16 [PMID: 20716517]
• Bhattacharya R, Cabral F. Molecular basis for class V beta-tubulin effects on microtubule assembly and paclitaxel resistance. J Biol Chem 2009-05-01 [PMID: 19282281]
• Handel EM, Alwin S, Cathomen T. Expanding or restricting the target site repertoire of zinc-finger nucleases: the inter-domain linker as a major determinant of target site selectivity. Mol Ther 2009-01-01 [PMID: 19002164] (Human)
• Nguyen CL, McLaughlin-Drubin ME, Munger K et al. Delocalization of the microtubule motor Dynein from mitotic spindles by the human papillomavirus E7 oncoprotein is not sufficient for induction of multipolar mitoses. Cancer Res 2008-11-01 [PMID: 18974113]
• Neklesa TK, Davis RW. Superoxide anions regulate TORC1 and its ability to bind Fpr1:rapamycin complex. Proc Natl Acad Sci U S A 2008-09-01 [PMID: 18812505]
• Cathomen T, Segal DJ, Brondani V, Muller-Lerch F. Generation and functional analysis of zinc finger nucleases. Methods Mol Biol 2008-01-01 [PMID: 18470651] (Human)
• Schakman O, Kalista S, BertrL et al. Role of Akt/GSK-3beta/beta-catenin transduction pathway in the muscle anti-atrophy action of insulin-like growth factor-I in glucocorticoid-treated rats. Endocrinology 2008-08-01 [PMID: 18467435]
• Wagenaar TR, Ojeda S, Moss B et al. Vaccinia virus A56/K2 fusion regulatory protein interacts with the A16 and G9 subunits of the entry fusion complex J Virol 2008-06-01 [PMID: 18353946]
  
  Details:
  This citation used the HRP version of this antibody.
• Chang M, Mukherjea D, Gobble RM et al. Glial cell missing 1 regulates placental growth factor (PGF) gene transcription in human trophoblast. Biol Reprod 2008-05-01 [PMID: 18160678]
• Liu Q, Dewi DA, Liu W et al. Distinct phosphorylation sites in the SST2A somatostatin receptor control internalization, desensitization, and arrestin binding. Mol Pharmacol 2008-02-01 [PMID: 17981995]
• Yin S, Cabral F, Veeraraghavan S et al. Amino acid substitutions at proline 220 of beta-tubulin confer resistance to paclitaxel and colcemid. Mol Cancer Ther 2007-10-01 [PMID: 17938271]
• Shen H, Korutla L, Champtiaux N et al. NAC1 regulates the recruitment of the proteasome complex into dendritic spines. J Neurosci 2007-08-01 [PMID: 17699672]
• Yang H, Cabral F. Heightened sensitivity to paclitaxel in Class IVa beta-tubulin-transfected cells is lost as expression increases. J Biol Chem 2007-09-01 [PMID: 17627938]
• Szczepek M, Brondani V, Buchel J et al. Structure-based redesign of the dimerization interface reduces the toxicity of zinc-finger nucleases. Nat Biotechnol 2007-07-01 [PMID: 17603476] (Human)
• Wang W, Mullikin-Kilpatrick D, Crandall JE et al. Nuclear factor I coordinates multiple phases of cerebellar granule cell development via regulation of cell adhesion molecules. J Neurosci 2007-06-01 [PMID: 17553984]
• Sancak Y, Thoreen CC, Peterson TR et al. PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase. Mol Cell 2007-03-01 [PMID: 17386266]
• Lu Y, Amleh A, Sun J et al. Ubiquitination and proteasome-mediated degradation of BRCA1 and BARD1 during steroidogenesis in human ovarian granulosa cells. Mol Endocrinol 2007-03-01 [PMID: 17185394]
• Lee JN, Song B, DeBose-Boyd RA et al. Sterol-regulated degradation of Insig-1 mediated by the membrane-bound ubiquitin ligase gp78. J Biol Chem 2006-12-01 [PMID: 17043353]
• Pereg Y, Lam S, Teunisse A et al. Differential roles of ATM- and Chk2-mediated phosphorylations of Hdmx in response to DNA damage. Mol Cell Biol 2006-09-01 [PMID: 16943424]
• Harper JM, Huynh MH, Coppens I et al. A cleavable propeptide influences Toxoplasma infection by facilitating the trafficking and secretion of the TgMIC2-M2AP invasion complex. Mol Biol Cell 2006-10-01 [PMID: 16914527]
• Glater EE, Megeath LJ, Stowers RS et al. Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent. J Cell Biol 2006-05-01 [PMID: 16717129]
• He L, Girijashanker K, Dalton TP et al. ZIP8, member of the solute-carrier-39 (SLC39) metal-transporter family: characterization of transporter properties. Mol Pharmacol 2006-07-01 [PMID: 16638970]
• Yamanaka T, Horikoshi Y, Izumi N et al. Lgl mediates apical domain disassembly by suppressing the PAR-3-aPKC-PAR-6 complex to orient apical membrane polarity. J Cell Sci 2006-05-01 [PMID: 16638806]
• Wang Y, Tian G, Cowan NJ et al. Mutations affecting beta-tubulin folding and degradation. J Biol Chem 2006-05-01 [PMID: 16554299]
• Wiesenthal V, Leutz A, Calkhoven CF. A translation control reporter system (TCRS) for the analysis of translationally controlled processes in the vertebrate cell. Nucleic Acids Res;34(3):e23. 2006-02-10 [PMID: 16473846]
• Alwin S, Gere MB, Guhl E et al. Custom zinc-finger nucleases for use in human cells. Mol Ther 2005-10-01 [PMID: 16039907]
• Wang Y, Cabral F. Paclitaxel resistance in cells with reduced beta-tubulin. Biochim Biophys Acta 2005-06-01 [PMID: 15950754]
• Bruzzaniti A, Neff L, Sanjay A et al. Dynamin forms a Src kinase-sensitive complex with Cbl and regulates podosomes and osteoclast activity. Mol Biol Cell 2005-07-01 [PMID: 15872089]
• Tanaka Y, Tamura K, Koide Y et al. The novel angiotensin II type 1 receptor (AT1R)-associated protein ATRAP downregulates AT1R and ameliorates cardiomyocyte hypertrophy. FEBS Lett 2005-03-01 [PMID: 15757644]
• Wu JJ, Choi LE, Guidotti G et al. N-linked oligosaccharides affect the enzymatic activity of CD39: diverse interactions between seven N-linked glycosylation sites. Mol Biol Cell 2005-04-01 [PMID: 15673609]
• Su JL, Fornwald J, Rivers P et al. A cell-based time-resolved fluorescence assay for selection of antibody reagents for G protein-coupled receptor immunohistochemistry. J Immunol Methods 2004-08-01 [PMID: 15345311]
• Chang TC, Yamashita A, Chen CY et al. UNR, a new partner of poly(A)-binding protein, plays a key role in translationally coupled mRNA turnover mediated by the c-fos major coding-region determinant. Genes Dev 2004-08-01 [PMID: 15314026]
• Bhattacharya R, Cabral F. A ubiquitous beta-tubulin disrupts microtubule assembly and inhibits cell proliferation. Mol Biol Cell 2004-07-01 [PMID: 15121885]
• Yamanaka T, Horikoshi Y, Sugiyama Y et al. Mammalian Lgl forms a protein complex with PAR-6 and aPKC independently of PAR-3 to regulate epithelial cell polarity. Curr Biol 2003-04-01 [PMID: 12725730]

Reviews for HA Tag Antibody (NB600-363) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, Other.

reviewed by: Tanya Kutyavin Simple Western Human 06/08/2018

Summary

• Application: Simple Western
• Sample Tested: Recombinant proteins
• Species: Human
• Lot: A8

reviewed by: Filipa Franquinho WB Human 05/04/2013

Summary

• Application: Western Blot
• Sample Tested: Hek 293T cell lysate
• Species: Human

reviewed by: Maria Andreou WB Other 09/26/2011

Summary

• Application: Western Blot
• Sample Tested: Xenopus laevis inejected embryo Lysates, Sample Amount: 30ug
• Species: Other
• Lot: A4

FAQs for HA Tag Antibody (NB600-363). (Showing 1 - 1 of 1 FAQs).

1. We are looking for a NON-RABBIT polyclonal anti-HA (hemagglutinin - YPYDVPDYA ) antibody for immunohistochemistry that works in 4% paraformaldehyde-fixed tissue. We have a transgenic mouse expressing an HA-tagged protein and want to perform double-labeling with another antibody made in rabbit. We have had no luck using mouse or rat monoclonal anti-HA antibodies. The non-rabbit polyclonal antibody would preferably be made in goat, although if you have other species let us know. It could be unconjugated or conjugated (eg fluorochrome or HRP). However, we are only interested in a product with demonstrated effectiveness for IHC in a published paper. Do you have any such products?
   • We do have other host species for this target and some that have been mentioned in publications. Unfortunately for our goat antibodies against this tag none of them have been validated in IHC-P, and for two we have shown they do not work. Please see this link to our goat anti-HA Epitope Tag antibodies. Since they work in ICC/IF I would assume as long as the tag is expressed in your tissues that you should still pick it up. In this case we have an Innovators Reward Program you can take advantage of for testing a new application to save some money. Here are the chicken anti-HA Epitope Tag antibodies. None of them have been tested in IHC-P as before, or that would be indicated with a positive result and the application on the datasheet or a negative results with the (-) beside the application. Another option for you as well would be going with a Rabbit polyclonal that we have available that has been well publicized and reviewed by customers (cat# NB600-363). I know you plan on double staining, but one way around this would be to use your other antibody and incubate with the secondary for detection first. After doing that you could have this one directly conjugated to your preferred detection probe and then pick up both of their signals since you would already have bound secondary to your first rabbit primary, there would not be any non-specific binding.