HAUS8 Antibody - BSA Free

Western Blot: HAUS8 Antibody [NBP2-42849] - Detection of cellular Hice1 and Hice1-GFP expressed in human osteosarcoma U2OS cells (left image), and Hice1 upon siRNA treatment (right image). Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10).

Immunocytochemistry/Immunofluorescence: HAUS8 Antibody [NBP2-42849] - Analysis of human osteosarcoma cell line U2OS, using HICE1 antibody at 1:50-1:200 dilution.

Immunohistochemistry-Paraffin: HAUS8 Antibody [NBP2-42849] - Human lymph tissue, using HICE1 antibody at 1:50 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: HAUS8 Antibody [NBP2-42849] - Analysis of A: His-Hice1 (2x) B: His-Hice1 (1x) 7.5% SDS PAGE diluted at 1:500.

Immunohistochemistry-Paraffin: HAUS8 Antibody [NBP2-42849] - Human lymph tissue, using HICE1 antibody at 1:50 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunoprecipitation: HAUS8 Antibody [NBP2-42849] - Analysis of Hice1 co-immunoprecipitated with other Augmin components HAUS6, HAUS2 and HAUS1 in U2OS cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HAUS8 Antibody - BSA Free Summary

• Immunogen: Recombinant protein corresponding to human HAUS8
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HAUS8
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:50-1:500
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for HAUS8 Antibody - BSA Free

• DGT4
• HAUS Augmin Like Complex Subunit 8
• HEC1/NDC80 interacting, centrosome associated 1
• HEC1/NDC80-Interacting Centrosome-Associated Protein 1
• Hec1-Interacting And Centrosome-Associated 1
• HICE1
• NY-SAR-48
• Sarcoma Antigen NY-SAR-48

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for HAUS8 Antibody (NBP2-42849)(1)

Publication using NBP2-42849

• Stimac V, Koprivec I, Manenica M et al. Augmin prevents merotelic attachments by promoting proper arrangement of bridging and kinetochore fibers eLife 2022-10-21 [PMID: 36269126] (WB, Human)
  
  Details:
  Dilution used in WB 1:1000

Bioinformatics

• Gene Symbol: HAUS8