hCG Antibody (1177B) [Unconjugated]

Recombinant Human Chorionic Gonadotropin alpha / beta (HCG) protein was serially diluted 2-fold and captured by Mouse Anti-Human Chorionic Gonadotropin alpha / beta (HCG) Monoclonal Antibody (Catalog # MAB97701) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human Chorionic Gonadotropin alpha / beta (HCG) Monoclonal Antibody (Catalog # MAB97702) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: ELISA
• Clone: 1177B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

hCG Antibody (1177B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Chinese Hamster Ovary cell line, CHO-derived human Chorionic Gonadotropin alpha / beta (HCG) and Human CG alpha
  Ser21-Gln165 and Ala25-Ser116
  Accession # P01233 and P01215
• Specificity: Detects human Chorionic Gonadotropin alpha / beta (HCG) and Human CG alpha in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for hCG Antibody (1177B) [Unconjugated]

• CGB
• CGB5
• CGB7
• CGB8
• CG-beta
• Choriogonadotropin subunit beta 3
• Choriogonadotropin
• Chorionic gonadotrophin chain beta
• Chorionic Gonadotropin alpha/beta
• Chorionic gonadotropin beta 3 subunit
• chorionic gonadotropin beta chain
• chorionic gonadotropin beta subunit 3
• chorionic gonadotropin chain beta
• hCGB
• LHB
• luteinizing hormone beta subunit

Background

HCG (human chorionic gonadotropin) is a member of the glycoprotein hormone (GPH) family within the cystine knot growth factor superfamily (1‑5). It is a heterodimer of a 23‑32 kDa unique subunit, CGb, with a 14‑22 kDa alpha subunit, CGa (common glycoprotein hormone alpha) that is shared with GPH family members lutropin (LH), follitropin (FSH) and thyroid stimulating hormone (TSH) (1, 2). CGb occurs only in higher primates, while the most closely related hormone, LHb, is expressed in all mammals (6). Human CGb and LHb share a receptor, LH/CG‑R or LHR, and show 86% aa sequence identity between aa 21 and 133, before diverging into a 32 aa, highly O‑glycosylated (CGb) or 8 aa (LHb) C‑terminal tail (2). Mature human CGa shares 69%‑73% aa identity with dog, rabbit, rat, mouse, cow, sheep, pig, cat and horse CGa. Each subunit form a cystine knot structure with three disulfide bridges (5). A “seat‑belt” loop of CGb wraps around CGa, stabilizing subunit non‑covalent association and conferring receptor selectivity (5). CGb is encoded by six clustered, nonallelic genes that encode identical, but differentially expressed, proteins (2, 7). HGC produced by cytotrophoblast cells in early pregnancy is hyperglycosylated and sialylated, increasing its acidity and half‑life (3, 4, 8). Forms with lower glycosylation are produced by syncytiotrophoblasts in continuing pregnancy, and in small amounts by the pituitary where it is also sulfated (4, 8). Free, variably glycosylated CGb subunits are also reported (3, 4). The primary role of HCG is to act as an autocrine factor to establish pregnancy and control placental growth and function. HCG has also been shown to induce the angiogenic factor, EG‑VEGF/PK1, and contribute to immune privilege by increasing circulating regulatory T cells and anti‑inflammatory cytokines IL‑10 and IL‑27, via cAMP signaling (9, 10). In addition to pregnancy, large amounts of HCG are produced in gestational trophoblastic diseases such as choriocarcinoma and hydatiform mole (3, 4). HCG may also be produced by ovarian and testicular germ cell tumors and advanced cancers that have dedifferentiated (3, 4).

1. Fiddes, J.C. and H.M. Goodman (1979) Nature 281:351.
2. Policastro, P. et al. (1983) J. Biol. Chem. 258:11492.
3. Stenman, U.H. et al. (2006) Human Reprod. Update 12:769.
4. Cole, L.A. (2012) Am. J. Cancer Res. 2:22.
5. Lapthorn, A.J. et al. (1994) Nature 369:455.
6. Maston, G.A. and M. Ruvolo (2002) Mol. Biol. Evol. 19:320.
7. Zimmerman, G. et al. (2012) Biol. Reprod. 86:1.
8. Kovalevskaya, G. et al. (2007) Mol. Cell. Endocrinol. 260-262:237.
9. Brouillet, S. et al. (2012) Cell. Mol. Life Sci. 69:1537.
10. Koldehoff, M. et al. (2011) J. Leukoc. Biol. 90:1017.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for hCG Antibody (MAB97701)(1)

Publication using MAB97701

• Yoshiki Nakashima, Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara et al. Induced hepatic stem cells are suitable for human hepatocyte production iScience 2022-10-21 [PMID: 36147945]

Bioinformatics

• Uniprot:
  • P01233 and P01215()