Helicobacter Pylori Antibody

Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody [NBP2-29479] - Formalin-fixed. paraffin-embedded human stomach stained with helicobacter pylori rabbit polyclonal antibody.

Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody [NBP2-29479] - Formalin-fixed, paraffin-embedded human stomach stained with Helicobacter pylori antibody (1:200), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues requires boiling tissue sections in citrate buffer, pH 6, or 1 mM EDTA, pH 8.0 for 10-20 min followed by cooling at RT for 20 min. Both methods allow for suitable detection, however using EDTA is the superior method.

• Reactivity: Ba
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Helicobacter Pylori Antibody Summary

• Description: 200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-33100)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Total sonicate of Helicobacter pylori
• Localization: Cell surface and cytoplasm
• Specificity: The spiral shaped bacterium Helicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-2ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Reactivity Notes

Helicobacter pylori.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for Helicobacter Pylori Antibody

• H. pylori

Background

Helicobacter pylori (H.pylori) is a gram-negative, microaerophil bacterium found in the stomach. Although Helicobacter pylori is present in the upper gastrointestinal track of more than 50% of the world's population, about 80% of people infected with Helicobacter pylori are asymptomatic. However, Helicobacter pylori is also strongly linked to disease and is thought to have evolved to penetrate the mucoid lining of the stomach. Penetrating the mucosal layer can cause considerable damage to mucosa, and chronic infection can result in or contribute to chronic gastritis, gastric ulcers as well as duodenal ulcers.

There is also strong evidence that Helicobacter pylori plays a causal role in the development of some stomach cancers, likely through chronic infection. However, Helicobacter pylori is also thought to play a role in normal stomach homeostasis which could help explain the large percent of people who have some level of Helicobacter pylori in their gut. The antibody can be used as an ancillary tool for identifying Helicobacter pylori by immunohistochemistry of tissue sections and may help determine if disease is linked to active infection. The antibody can also be used to study the presence of Helicobacter pylori in healthy tissue and may be a useful biomarker for further understanding the role of microbes in stomach homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.