Herpes Simplex Virus 1 Antibody

Immunohistochemistry-Paraffin: Herpes Simplex Virus 1 Antibody [NB120-9533] - Formalin fixed paraffin embedded tissue stained with HSV I antibody (NB120-9533).

• Reactivity: V-Vi
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Herpes Simplex Virus 1 Antibody Summary

• Immunogen: Whole rabbit corneal cells, infected with herpes simplex virus type I (strain Mac Intyre), were solubilized with detergent.
• Localization: Cytoplasmic, nuclear
• Specificity: This antibody reacts with Herpes Simplex Virus 1 (HSV1) specific antigens and with antigens common to Herpes Simplex Virus 1 (HSV1) and II virus. It reacts with all the major glycoproteins present in the viral envelope as well as with at least one core protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:75-1:200
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:75-1:200
• Application Notes: IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS (pH 7.4), 0.2% BSA, Tween-20
• Preservative: 0.05% Sodium Azide
• Purity: Affinity purified

Alternate Names for Herpes Simplex Virus 1 Antibody

• Herpes simplex virus 1
• Herpes simplex virus type 1
• Herpes simplex virus
• HSV 1
• Human herpesvirus 1
• Human herpesvirus type 1

Background

Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Herpes Simplex Virus 1 Antibody (NB120-9533). (Showing 1 - 2 of 2 FAQ).

1. What is the buffer composition and azide concentration for NB120-9533 (HSV1 Antibody)?
   • The buffer is PBS supplemented with 2% BSA and 0.05% sodium azide
2. The data sheet for this antibody says:"This reacts with Herpes Simplex Virus 1 (HSV1) specific antigens and with antigens common to Herpes Simplex Virus 1 (HSV1) and II virus."Is this ab useful for HSV1 and HSV2?
   • this antibody does recognize both the HSV1 and HSV2. So, in regards to HSV1 and IHC, it is accepted that it will cross-react with the common antigens between HSV I and II, along with the unique antigen for HSV1, HSV2 does the same. When used clinically, end users typically cocktail the two.Unfortunately, this limits the ability of either antibody to be used as a research IHC tool when studying only HSV1 or HSV2 – the antibodies commercially available for IHC will not distinguish between the two specifically.