HIF-1 alpha Antibody - BSA Free

ChIP-qPCR assay of HIF-1 binding to hypoxia response element (HRE) of the PKM2 gene in human MDA-MB-231 breast cancer cells treated with vehicle or DMOG (HIF inducer) for 8 hours. Negative control: immunoprecipitation was performed with IgG. Image courtesy of Gregg Semenza, Johns Hopkins University School of Medicine.

ChIP-qPCR assay of HIF-1 binding to hypoxia response element (HRE) of the PKM2 gene in human MDA-MB-231 breast cancer cells treated with vehicle or DMOG (HIF inducer) for 8 hours. Negative control: immunoprecipitation was performed with IgG. Image courtesy of Gregg Semenza, Johns Hopkins University School of Medicine.

Normoxic pre-treatment sensitizes glioma cells to radiation after graded chronic hypoxic (GCH) exposure. The results of anchorage-independent colony forming assays are shown for U87, U87-luc, GL261 glioma cells and 0308 GSCs after 5 Gy radiation exposure under varying O2 conditions. For easier comparisons among cell types, raw values are expressed as a % of the cell type's negative (non-irradiated) control and the means & SEMs are plotted. Each result represents at least 3 independent samples, plated in triplicate. Holm-Sidak comparisons for multiple groups were used for statistical comparisons of raw values (*p N) localization in three microscopic viewing areas were counted as shown in the lower panel. The total counted cell numbers ranged from 35 to 64. The experiments were repeated more than twice, except for the cell localization count, & the results were reproduced. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28775317), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hypoxia signaling & cytotoxicity to CdCl2. (a) Wild-type (WT) cells were left untreated (Ctrl) or exposed to 150 μM CoCl2 (Co2+), 1, 5 or 10 μM CdCl2 (Cd2+) or 150 μM CoCl2 & 10 μM CdCl2 for 24 hours. HIF1 alpha −/− cell extract treated with 150 μM CoCl2 was used as a negative control. Nuclear protein was extracted & separated by SDS-PAGE, transferred to nitrocellulose membrane & probed with a HIF1 alpha (upper panel) or beta -actin (lower panel) specific antibody. The bands observed in the cadmium only WT cells are nonspecific as they are also observed in the HIF1 alpha −/− cells. (b) BNip3 mRNA expression levels were analyzed by qRT-PCR in wild type (WT, white bars) & HIF1 alpha −/− cells (black bars). Cells were left untreated (0), or exposed to 5 μM CdCl2 (Cd2+) or 150 μM CoCl2 (Co2+) for 24 hours. Each value was normalized to the control level in the corresponding cell line. *P 0.05; *p 60 recipients/genotype). (C) When 500 WT & KO cells were implanted into the mammary fat pad, the median time until 50% of recipients developed tumors > 500 mm3 was 64 days for WT mice & 127 days for KO mice (n = 14 recipients/genotype, P < 0.001, logrank test). (D) Western blot for HIF-1 alpha in three independent tumors (500 to 750 mm3) per genotype. WT & KO cells that were cultured for 6 hours under hypoxic conditions served as positive & negative controls, respectively. CRM, cross-reactive material. MTEC, mammary tumor epithelial cell. (E) Mean fold change ± SEM in expression of HIF-1 targets in KO tumors as determined by qRT-PCR (n = 5 tumors/genotype). Decreased gene expression in KO tumors is presented as a negative fold-change relative to WT tumors. (F) An increase in Ki67+ cells in KO tumors is balanced by an increase in caspase 3-positive cells (n = 5 tumors/genotype, *P < 0.05, Student's t-test). Representative immunostaining images are shown in Additional file 2 Figure S4. Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3087), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Effect of HIF-1 alpha silencing on alpha -MSH-induced apoptosis in B16-F10 melanoma cells during hypoxia.a Cells were transfected with shLacZ or shHIF-1 alpha plasmids for 48 h before harvest. HIF-1 alpha shRNA reduced the basal HIF-1 alpha mRNA & protein expression levels. b, c Relative mRNA expression levels were analyzed by real-time PCR. Data are expressed as fold change compared with control (means ± SD of triplicate experiments). d Cell lysates were analyzed by immunoblot using the indicated antibodies. beta -Actin was used as an internal control for loading & transfer. e The population of apoptotic cells was analyzed by flow cytometry & qualified as mean ± SD from triplicate experiments. *p < 0.05, **p < 0.01. Knockdown of HIF-1 alpha diminished alpha -MSH-induced apoptosis in B16-F10 melanoma cells during hypoxia Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30062060), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hif1 alpha expression in neonatal & adult testis.(A) Section of testis from 5-day old (P5) male new born pups showing Hif1 alpha expression in MVH+ gonocytes within the seminiferous tubules (top) & negative control images without primary antibodies (bottom). Scale bar: 50μm. (B) Western blot analysis of Hif1 alpha expression in P5 testes (left) compared to extract of the adult brain sub-ventricular zone (SVZ) (right). Loading control ( beta -Actin) is shown below. (C) Section of adult (3 month old) testis showing Hif1 alpha expression in spermatogonia. Scale bar: 30μm. (D) Western blot analysis of whole adult testis. HEK293 cells treated with DFX were used as a positive control & intestinal tissue was used as a negative control. Loading control ( beta -Actin) is shown below. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27148974), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Expression of hypoxia-inducible factor (HIF) proteins after bilateral renal ischemia or sham ischemia. Immunoblots for HIF-1 alpha (A–C) & HIF-2 alpha (D–F) of tissue extracts from the cortex & outer & inner medulla of the left kidneys of rats 24 h & 5 days following recovery from either sham ischemia (○) or bilateral renal ischemia (●); n = 6 per group. G: typical image of the gel following electrophoresis. H: typical image of the nitrocellulose membrane following transfer. Values are expressed as medians (25th percentile, 75th percentile). Paired comparisons were performed using the Mann-Whitney U-test. Because paired comparisons were made at two time points, P values were conservatively adjusted using the Dunn-Sidak method with k = 2. PTr, PT, & PTr*T are the outcomes of two-way analysis of variance on ranking with the factors treatment (Tr) & time (T). AU, arbitrary unit; I1, 24 h after ischemia; I5, 5 days after ischemia; S1, 24 h after sham ischemia; S5, 5 days after sham ischemia. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30110566), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hypoxia reduces inflammatory signaling pathways & NLRP3 expression & induces autophagy in IECs. a HT-29 cells were subjected to normoxia & hypoxia at the indicated times in the absence or presence of 10 µg/ml LPS. Autophagy was measured by variations in the ratio of LC3-II/LC3-I & the total amount of LC3 (LC3-I plus LC3-II) relative to beta -actin. Results are representative of two independent experiments. b, c & d HT-29 cells were subjected to normoxia & hypoxia for the indicated periods in the absence or presence of 10 µg/ml LPS, followed by transcript analysis. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-test. Results represent mean + s.e.m. of two independent experiments done in triplicate, *P < 0.05; **P < 0.01; ***P < 0.001; ns not significant. e HT-29 cells were subjected to normoxia or hypoxia in the presence & absence of 10 µg/ml LPS & 20 µM of MG132. Results are representative of two independent experiments. f & g Putative binding sites for HIF-1 alpha & NF-kappa B were found in the p62 f & NLRP3 g promoters using Genomatix software tools. Numbers under the boxes indicate the distance from the transcription start site. HT-29 cells were subjected to normoxia (21% O2) or hypoxia (0.2% O2) for 6 h & 24 h. ChIP analysis was performed using antibodies against HIF-1 alpha & NF-kappa B for immunoprecipitation. PCR was performed using the promoter-specific primers for the p62 f & NLRP3 g promoter binding sites of HIF-1 alpha & NF-kappa B. Aliquots taken prior to immunoprecipitation were used as input control. PCR products were run on 2% agarose gel. The results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28740109), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Transient hyperoxia does not sensitize normal human astrocytes to radiation.Results are shown for the colony forming assays for all previously assayed cell lines & a normal human astrocyte cell line (Astro). Cells were continuously maintained under normoxic conditions (NOx) or exposed to 25 min of hyperoxia (50% O2) & then returned to normoxic conditions for 25 min (HyperOx) before being treated with a 5 Gy dose of radiation. To allow for ease of comparisons among cell types, raw values are expressed as a percentage of the corresponding cells type’s negative (non-irradiated) control & the means & SEMs are plotted. Each result represents three independent samples, plated in triplicate (*p<0.05, Student’s t-test). Also shown are Western blots of nuclear HIF-1 alpha at the time of irradiation for each cell line. Corresponding Western blots of lamin A/C are shown as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25350400), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - YC-1 attenuates pro-inflammatory differentiation of macrophages. (a,b) qRT-PCR analyses, revealing mRNA expression of the pro-inflammatory M1-differentiation markers NOS2 (a) & IL-6 (b) in murine J774A.1 macrophages under normoxia (left bars) & hypoxia (right bars). LPS exposure was carried out to induce M1-polarization in presence of YC-1 or vehicle control (DMSO; **P ≤ 0.01, n = 9). (c,d) Western blots of nuclear extracts, revealing protein levels of HIF-1 alpha (c) & HIF-2 alpha (d) in J774.A1 macrophages treated with vehicle (DMSO) or YC-1 under normoxic (left) or hypoxic (right) culture conditions. Simultaneous LPS-treatment was performed to induce pro-inflammatory activation. Note LPS-induced stabilization of HIF-1 alpha - (c), but not HIF-2 alpha -levels (d), & reversal of LPS-induced HIF-1 alpha -stabilization by YC-1 (c). Histone H3 was used as a loading control. (e,f) qRT-PCR analyses, revealing mRNA expression of the pro-inflammatory M1-differentiation markers NOS2 (e) & IL-6 (f) in BMDMs under normoxia (left bars) & hypoxia (right bars). LPS exposure was carried out to induce M1-polarization in presence of YC-1 or vehicle control (DMSO; ***P ≤ 0.001, **P ≤ 0.01, n = 6). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-13638-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Po, Ca, Fi, Gt, Ha, Pm, Rb, Ze
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP, WB, ChIP, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HIF-1 alpha Antibody - BSA Free Summary

• Immunogen: This HIF-1 alpha antibody was developed against a fusion protein including amino acids 530 - 825 of the mouse HIF-1 alpha protein [Uniprot# Q61221].
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HIF1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Flow Cytometry 1:10 - 1:1000. Use reported in scientific literature (PMID 21917971)
  • Immunoblotting reported in scientific literature (PMID 28506759)
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry Whole-Mount reported in scientific literature (PMID 27148974)
  • Immunohistochemistry 5 - 10 ug/mL
  • Immunohistochemistry-Frozen 5 - 10 ug/mL
  • Immunohistochemistry-Paraffin 5 - 10 ug/mL
  • Immunoprecipitation 1:10 - 1:500
  • Knockdown Validated
  • Simple Western
  • Western Blot 1-2ug/ml
• Application Notes: WB recognizes a band in hypoxic samples at ~115 kDa. HIF-1 alpha protein (NBC1-18422) has been successfully used for antibody preadsorption/blocking assay in IHC-P with HIF-1 alpha antibody NB100-479.
• Theoretical MW: 93 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Pancreas Whole Tissue Lysate (Adult Whole Normal)	Loading...
  COS-7 Nuclear Hypoxic Induced Cell Lysate	Loading...
  Human Bone Marrow Whole Tissue Lysate (Adult Whole Diabetes)	Loading...
  HeLa CoCl2 treated/Untreated Cell Lysate	Loading...
  HepG2 CoCl2 treated/Untreated Cell Lysate	Loading...
  HeLa Hypoxic / Normoxic Cell Lysate	Loading...
  HepG2 Hypoxic / Normoxic Cell Lysate	Loading...
  HIF-1 alpha Knockout CoCl2-treated/untreated HeLa Cell Lysate	Loading...
  HIF-1 alpha Knockout Hypoxic-treated/untreated HeLa Cell Lysate	Loading...
  NIH 3T3 CoCl2 treated/Untreated Cell Lysate	Loading...
  L929 CoCl2 treated/Untreated Cell Lysate	Loading...
  Raw 264.7 CoCl2 treated/Untreated Cell Lysate	Loading...
  PC12 CoCl2 treated/Untreated Cell Lysate	Loading...
  PC12 Hypoxic / Normoxic Cell Lysate	Loading...
  Raw 264.7 Hypoxic / Normoxic Cell Lysate	Loading...
  L929 Hypoxic / Normoxic Cell Lysate	Loading...
  NIH 3T3 Hypoxic / Normoxic Cell Lysate	Loading...

• Control Peptide:

  HIF-1 alpha Recombinant Protein (NBC1-18422)

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Reactivity Notes

Use in Zebrafish reported in scientific literature (PMID:35457018).Use in Mouse reported in scientific literature (PMID:33727588). Use in Goat reported in scientific literature (PMID:21599540).

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HIF-1 alpha Antibody - BSA Free

• AINT
• anti-HIF-1 alpha
• anti-HIF1A
• ARNT interacting protein
• ARNT-interacting protein
• Basic-helix-loop-helix-PAS protein MOP1
• BHLHE78
• Class E basic helix-loop-helix protein 78
• HIF 1A
• HIF1 alpha
• HIF-1 alpha
• HIF1
• HIF1A
• HIF-1a
• HIF-1alpha
• HIF-1-alpha
• HIF1-alpha
• hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha
• hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• hypoxia-inducible factor 1-alpha
• Member of PAS protein 1
• member of PAS superfamily 1
• MOP1
• PAS domain-containing protein 8
• PASD8

Background

Hypoxia contributes to the pathophysiology of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease (1). In cancer and particularly solid tumors, hypoxia plays a critical role in the regulation of genes involved in stem cell renewal, epithelial to mesenchymal transition (EMT), metastasis and angiogenesis. In the tumor microenvironment (TME), hypoxia influences the properties and function of stromal cells (e.g., fibroblasts, endothelial and immune cells) and is a strong determinant of tumor progression (2,3).

HIF-1 or hypoxia inducible factor 1 (predicted molecular weight 93kDa), is a transcription factor commonly referred to as a "master regulator of the hypoxic response" for its central role in the regulation of cellular adaptations to hypoxia. In its active form under hypoxic conditions, HIF-1 is stabilized by the formation of a heterodimer of HIF-1 alpha and ARNT/HIF-1 beta subunits. Nuclear HIF-1 engages p300/CBP for binding to hypoxic response elements (HREs). This process induces transcription and regulation of genes including EPO, VEGF, iNOS2, ANGPT1 and OCT4 (4,5).

Under normoxic conditions, the HIF-1 alpha subunit is rapidly targeted and degraded by the ubiquitin proteasome system. This process is mediated by prolyl hydroxylase domain enzymes (PHDs), which catalyze the hydroxylation of key proline residues (Pro-402 and Pro-564) within the oxygen-dependent degradation domain of HIF-1 alpha. Once hydroxylated, HIF-1 alpha binds the von Hippel-Lindau tumor suppressor protein (pVHL) for subsequent ubiquitination and proteasomal degradation (4). pVHL dependent regulation of HIF-1 alpha plays a role in normal physiology and disease states. Regulation of HIF-1 alpha by pVHL is critical for the suppressive function of FoxP3+ regulatory Tcells (6). Repression of pVHL expression in chronic lymphocytic leukemia (CLL) B cells leads to HIF-1 alpha stabilization and increased VEGF secretion (7).

References

1. Semenza, G. L., Agani, F., Feldser, D., Iyer, N., Kotch, L., Laughner, E., & Yu, A. (2000). Hypoxia, HIF-1, and the pathophysiology of common human diseases. Advances in Experimental Medicine and Biology.

2. Muz, B., de la Puente, P., Azab, F., & Azab, A. K. (2015). The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia. https://doi.org/10.2147/hp.s93413

3. Huang, Y., Lin, D., & Taniguchi, C. M. (2017). Hypoxia inducible factor (HIF) in the tumor microenvironment: friend or foe? Science China Life Sciences. https://doi.org/10.1007/s11427-017-9178-y

4. Koyasu, S., Kobayashi, M., Goto, Y., Hiraoka, M., & Harada, H. (2018). Regulatory mechanisms of hypoxia-inducible factor 1 activity: Two decades of knowledge. Cancer Science. https://doi.org/10.1111/cas.13483

5. Dengler, V. L., Galbraith, M. D., & Espinosa, J. M. (2014). Transcriptional regulation by hypoxia inducible factors. Critical Reviews in Biochemistry and Molecular Biology. https://doi.org/10.3109/10409238.2013.838205

6. Lee, J. H., Elly, C., Park, Y., & Liu, Y. C. (2015). E3Ubiquitin Ligase VHL Regulates Hypoxia-Inducible Factor-1 alpha to Maintain Regulatory T Cell Stability and Suppressive Capacity. Immunity. https://doi.org/10.1016/j.immuni.2015.05.016

7. Ghosh, A. K., Shanafelt, T. D., Cimmino, A., Taccioli, C., Volinia, S., Liu, C. G., ... Kay, N. E. (2009). Aberrant regulation of pVHL levels by microRNA promotes the HIF/VEGF axis in CLL B cells. Blood. https://doi.org/10.1182/blood-2008-10-185686

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HIF-1 alpha Antibody (NB100-479)(538)

Publications using NB100-479

• Ming Chen, Yekai Wang, Roopa Dalal, Jianhai Du, Douglas Vollrath Alternative oxidase blunts pseudohypoxia and photoreceptor degeneration due to RPE mitochondrial dysfunction Proceedings of the National Academy of Sciences of the United States of America 2024-06-12 [PMID: 38865272]
• Lee E, Jung Y, Park Y et al. A distinct astrocyte subtype in the aging mouse brain characterized by impaired protein homeostasis Nature Aging Aug 1 2022 (IHC-Fr, Mouse)
  
  Details:
  Dilution used for IHC 1:300
• Hertzog N, Duman M, Bochud M et Al. Hypoxia-induced conversion of sensory Schwann cells into repair cells is regulated by HDAC8 Nat Commun 2025-01-09 [PMID: 39779705]
• Song C, Sawall JK, Ji X et Al. Tgf? signaling stimulates glycolysis to promote the genesis of synovial joint interzone in developing mouse embryonic limbs Sci Adv 2025-01-10 [PMID: 39772668]
• Ahn JH, da Silva Pedrosa M, Lopez LR, Tibbs TN et Al. Intestinal E. coli-produced yersiniabactin promotes profibrotic macrophages in Crohn's disease Cell Host Microbe 2024-12-19 [PMID: 39701098]
• Benoit A, Lequeux A, Harter P et al. Atypical chemokine receptor 2 expression is directly regulated by hypoxia inducible factor-1 alpha in cancer cells under hypoxia Scientific Reports 2024-11-04 [PMID: 39496762]
• Yang G, Xiang J, Yang X et al. Nuclear translocation of SIRT4 mediates deacetylation of U2AF2 to modulate renal fibrosis through alternative splicing-mediated upregulation of CCN2 eLife 2024-11-04 [PMID: 39495216]
• Soler-Agesta R, Moreno-Loshuertos R, Yim CY et al. Cancer cell-selective induction of mitochondrial stress and immunogenic cell death by PT-112 in human prostate cell lines. Journal of translational medicine 2024-10-12 [PMID: 39394618]
• Li M, Jin H, Zhao Y et Al. PHD2 safeguards modest mesendoderm development Commun Biol 2024-09-07 [PMID: 39244636]
• Ohgaki R, Hirase Y, Xu M et Al. LAT1 expression in colorectal cancer cells is unresponsive to HIF-1/2? accumulation under experimental hypoxia Sci Rep 2024-08-23 [PMID: 39179631]
• Chung YJ, Hoare Z, Baark F et Al. Elevated Na is a dynamic and reversible modulator of mitochondrial metabolism in the heart Nat Commun 2024-05-20 [PMID: 38769288]
• Bernice Woon Li Lee, You Heng Chuah, Jeehyun Yoon, Oleg V. Grinchuk, Yajing Liang, Jayshree L. Hirpara, Yating Shen, Loo Chien Wang, Yan Ting Lim, Tianyun Zhao, Radoslaw M. Sobota, Tseng Tsai Yeo, Andrea Li Ann Wong, Kejia Teo, Vincent Diong Weng Nga, Bryce Wei Quan Tan, Toshio Suda, Tan Boon Toh, Shazib Pervaiz, Zhewang Lin, Derrick Sek Tong Ong METTL8 links mt-tRNA m 3 C modification to the HIF1 alpha /RTK/Akt axis to sustain GBM stemness and tumorigenicity Cell Death & Disease 2024-05-14 [PMID: 38744809]
• Yao F, Chu M, Xi G et Al. Single-embryo transcriptomic atlas of oxygen response reveals the critical role of HIF-1? in prompting embryonic zygotic genome activation Redox Biol 2024-05-05 [PMID: 38593632]
• Lyu Y, Yang Y, Talwar V et al Hypoxia-inducible factor 1 recruits FACT and RNF20/40 to mediate histone ubiquitination and transcriptional activation of target genes Cell Rep 2024-03-22 [PMID: 38517892] (ChIP, Human)
• Ahmed A. Elbassiouny, Leslie T. Buck, Luis E. Abatti, Jennifer A. Mitchell, William G.R. Crampton, Nathan R. Lovejoy, Belinda S.W. Chang Evolution of a novel regulatory mechanism of hypoxia inducible factor in hypoxia-tolerant electric fishes The Journal of Biological Chemistry 2024-02-05 [PMID: 38325739]
• Gille AS, Givelet M, Pehlic D et Al. Impact of the hypoxic microenvironment on spermatogonial stem cells in culture Front Cell Dev Biol 2024-01-18 [PMID: 38304612]
• Jin, Y;Peng, Y;Xu, J;Yuan, Y;Yang, N;Zhang, Z;Xu, L;Li, L;Xiong, Y;Sun, D;Pan, Y;Wu, R;Fu, J; LUBAC promotes angiogenesis and lung tumorigenesis by ubiquitinating and antagonizing autophagic degradation of HIF1? Oncogenesis 2024-01-25 [PMID: 38272870]
• Duc-Vinh Pham, Thi-Kem Nguyen, Bao-Loc Nguyen, Jong-Oh Kim, Jee-Heon Jeong, Inho Choi, Pil-Hoon Park Adiponectin restores the obesity-induced impaired immunomodulatory function of mesenchymal stromal cells via glycolytic reprogramming Acta Pharmaceutica Sinica. B 2023-10-28 [PMID: 38261813]
• Kunke, M;Knöfler, H;Dahlke, E;Zanon Rodriguez, L;Böttner, M;Larionov, A;Saudenova, M;Ohrenschall, GM;Westermann, M;Porubsky, S;Bernardes, JP;Häsler, R;Magnin, JL;Koepsell, H;Jouret, F;Theilig, F; Targeted deletion of von-Hippel-Lindau in the proximal tubule conditions the kidney against early diabetic kidney disease Cell death & disease 2023-08-26 [PMID: 37626062] (Western Blot, Mouse)
• Yamazaki T, Mimura I, Miura R et al. Dznep, a histone modification inhibitor, inhibits HIF1 alpha binding to TIMP2 gene, by reducing open chromatin area Research Square 2022-08-25 (Chemotaxis)
  
  Details:
  Dilutions used for ChIP 1:50
• Yong Hwan Kim, Na Yeon Park, Doo Sin Jo, Ji-Eun Bae, Joon Bum Kim, Kyuhee Park, Kwiwan Jeong, Pansoo Kim, Eunbyul Yeom, Dong-Hyung Cho Inhibition of VHL by VH298 Accelerates Pexophagy by Activation of HIF-1 alpha in HeLa Cells. Molecules (Basel, Switzerland) 2024-01-24 [PMID: 38257395]
• Correa-Gallegos D, Ye H, Dasgupta B et al. CD201+ fascia progenitors choreograph injury repair Nature 2023-11-01 [PMID: 37968392] (IHC-P, Mouse)
  
  Details:
  1:100 IHC-P dilution
• Wang Y, Halawani D, Estill M et al. Aryl hydrocarbon receptor restricts axon regeneration of DRG neurons in response to injury bioRxiv : the preprint server for biology 2023-11-05 [PMID: 37961567] (WB, Mouse)
• Wang N, Su X, Sams D et al. P300/CBP Regulates HIF-1 Dependent Sympathetic Activation and Hypertension by Intermittent Hypoxia American journal of respiratory cell and molecular biology 2023-10-24 [PMID: 37874694]
  
  Details:
  DNA immunoprecipitation
• Zhu X, Chen J, Li W et al. Hypoxia-responsive CAR-T cells exhibit reduced exhaustion and enhanced efficacy in solid tumors Cancer research 2023-10-24 [PMID: 37874330]
• Yamazaki T, Mimura I, Kurata Y et al. Dznep, a histone modification inhibitor, inhibits HIF1? binding to TIMP2 gene and suppresses TIMP2 expression under hypoxia Physiol Rep 2023-09-14 [PMID: 37710084] (Western Blot, Mouse)
• Wang D, Zhu Z, Fu Y et al. Bromodomain-containing protein 4 activates androgen receptor transcription and promotes ovarian fibrosis in PCOS Cell reports 2023-09-04 [PMID: 37669164] (IP, Rat)
• Maduabuchi, WO;Tansi, FL;Heller, R;Hilger, I; Hyperthermia Influences the Secretion Signature of Tumor Cells and Affects Endothelial Cell Sprouting Biomedicines 2023-08-12 [PMID: 37626752] (Western Blot, Mouse)
• Vellama H, Eskla KL, Eichelmann H et al. VHL-deficiency leads to reductive stress in renal cells Free radical biology & medicine 2023-07-26 [PMID: 37506952] (WB, Human)
  
  Details:
  1:1000 WB dilution
• Ouhaddi Y, Dalisson B, Rastinfard A et al. Necrosis reduction efficacy of subdermal biomaterial mediated oxygen delivery in ischemic skin flaps Biomaterials advances 2023-06-16 [PMID: 37392519] (IHC, Rat)
• Mawambo G, Oubaha M, Ichiyama Y et al. HIF1?-dependent hypoxia response in myeloid cells requires IRE1? Journal of neuroinflammation 2023-06-21 [PMID: 37344842] (WB, IP, Mouse)
• Samuel T, Rapic S, O'Brien C et al. Quantitative intravital imaging for real-time monitoring of pancreatic tumor cell hypoxia and stroma in an orthotopic mouse model Science advances 2023-06-09 [PMID: 37285434] (WB, Human)
  
  Details:
  1:50 WB dilution
• Pathmanapan, S;Poon, R;De Renshaw, TB;Nadesan, P;Nakagawa, M;Seesankar, GA;Ho Loe, AK;Zhang, HH;Guinovart, JJ;Duran, J;Newgard, CB;Wunder, JS;Alman, BA; Mutant IDH regulates glycogen metabolism from early cartilage development to malignant chondrosarcoma formation Cell reports 2023-06-01 [PMID: 37267108] (IP, ChIP, IHC, WB, Human, Mouse)
• Zhang H, Wang J, Prakash J et al. Three-way Cell-based Screening of Antistress Compounds: Identification, Validation, and Relevance to Old-age Related Pathologies The journals of gerontology. Series A, Biological sciences and medical sciences 2023-04-15 [PMID: 37061830]
• Cheng Y, Li J, Zhang X et al. Protective Effect of Qingchang Wenzhong Decoction on Colitis and Colitis-Related Carcinogenesis by Regulating Inflammation and Intestinal Fibrosis Journal of inflammation research 2023-04-07 [PMID: 37056910] (IHC-P, Mouse)
• Shin J, Nunomiya A, Gonda K, Nagatomi R Specification of skeletal muscle fiber-type is determined by the calcineurin/NFATc1 signaling pathway during muscle regeneration Biochemical and Biophysical Research Communications 2023-03-01 [PMID: 37031590] (Western Blot, Mouse)
• Okuda C, Ueda Y, Muroi M et al. Allantopyrone A interferes with the degradation of hypoxia-inducible factor 1? protein by reducing proteasome activity in human fibrosarcoma HT-1080 cells The Journal of antibiotics 2023-03-30 [PMID: 36997727]
• Lerma Clavero A, Boqvist PL, Ingelshed K et al. MDM2 inhibitors, nutlin-3a and navtemadelin, retain efficacy in human and mouse cancer cells cultured in hypoxia Scientific Reports 2023-03-20 [PMID: 36941277] (Western Blot, Mouse)
• Zuo Q, Yang Y, Lyu Y, Yang C, Chen C, Salman S, Huang T, Wicks E, Jackson W, Datan E, Qin W, Semenza G Plexin-B3 expression stimulates MET signaling, breast cancer stem cell specification, and lung metastasis. Cell Rep, 2023-02-28;42(3):112164. 2023-02-28 [PMID: 36857181] (Chromatin Immunoprecipitation, Immunoprecipitation)
• Liu Y, Gu Y, Ng S Et Al. Circulating levels of hydroxylated bradykinin function as an indicator of tissue HIF-1 alpha expression Science Bulletin 2020-01-01 [PMID: 36738075] (EA, WB, Human)
• Seike K, Kiledal A, Fujiwara H et al. Ambient oxygen levels regulate intestinal dysbiosis and GVHD severity after allogeneic stem cell transplantation Immunity 2023-02-01 [PMID: 36736321] (IHC-P, Mouse)
  
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  Dilution used 1:1000
• Maki T, Sawahata M, Uta D et al. Chronic Treatment with A?42 with a Toxic Conformer and LPS Induces Inflammatory Responses in BV-2 Microglia with Dysregulation of Hypoxia-Inducible Factor Expression Biological & pharmaceutical bulletin 2023-02-01 [PMID: 36724966] (WB, Mouse)
  
  Details:
  Dilution used in WB 1:1000
• Silwal P, Kim YJ, Lee YJ et al. Chemical mimetics of the N-degron pathway alleviate systemic inflammation by activating mitophagy and immunometabolic remodeling Experimental & molecular medicine 2023-02-01 [PMID: 36720915] (WB, Mouse)
  
  Details:
  Dilution used in WB 1:1000
• Karreman MA, Bauer AT, Solecki G et al. Active Remodeling of Capillary Endothelium via Cancer Cell-Derived MMP9 Promotes Metastatic Brain Colonization Cancer research 2023-04-14 [PMID: 36652557]
• Chen Z, Bordieanu B, Kesavan R et al. Lactate metabolism is essential in early-onset mitochondrial myopathy Science Advances 2023-01-06 [PMID: 36598990] (Western Blot, Mouse)
• Kostyuk SV, Ershova ES, Martynov AV et al. In Vitro Analysis of Biological Activity of Circulating Cell-Free DNA Isolated from Blood Plasma of Schizophrenic Patients and Healthy Controls-Part 2: Adaptive Response Genes 2022-12-04 [PMID: 36553550] (FLOW)
• Stobart JL, Erlebach E, Gl�ck C et al. Altered hemodynamics and vascular reactivity in a mouse model with severe pericyte deficiency Journal of Cerebral Blood Flow & Metabolism 2023-05-01 [PMID: 36545806] (Western Blot, Mouse)
• Chen Y, Zhang S, Ye L et al. An Acute Bout of Exercise Suppresses Appetite via Central Lactate Metabolism Neuroscience 2022-12-15 [PMID: 36529295] (Western Blot, Mouse)
• Yang Y, Chen C, Zuo Q Et al. NARF is a hypoxia-induced coactivator for OCT4-mediated breast cancer stem cell specification Sci Adv 2022-12-09 [PMID: 36490339] (Chemotaxis, Human)
  
  Details:
  Citation using the DyLight 405 version of this antibody.
• Chidlow G, Chan WO, Wood JPM, Casson RJ. Investigations into photoreceptor energy metabolism during experimental retinal detachment Frontiers in Cellular Neuroscience 2022-11-18 [PMID: 36467607] (Western Blot, Mouse)
• Kim D, Khin PP, Lim OK, Jun HS LPA/LPAR1 signaling induces PGAM1 expression via AKT/mTOR/HIF-1 alpha pathway and increases aerobic glycolysis, contributing to keratinocyte proliferation Life sciences 2022-12-15 [PMID: 36400203] (IHC-P, WB, Mouse, Human)
• Maki T, Sawahata M, Akutsu I et al. APP Knock-In Mice Produce E22P-AB Exhibiting an Alzheimer’s Disease-like Phenotype with Dysregulation of Hypoxia-Inducible Factor Expression International Journal of Molecular Sciences 2022-10-31 [PMID: 36362046] (WB, Mouse)
• Yegen CH, Haine L, Da Costa Ferreira K et al. A New Model of Acute Exacerbation of Experimental Pulmonary Fibrosis in Mice Cells 2022-10-26 [PMID: 36359778] (Western Blot, Mouse)
• Bao S, Chen Z, Qin D et al. Single-cell profiling reveals mechanisms of uncontrolled inflammation and glycolysis in decidual stromal cell subtypes in recurrent miscarriage Human reproduction (Oxford, England) 2022-11-10 [PMID: 36355621]
• Qian L, Rawashdeh O, Kasas L et al. Cholinergic basal forebrain degeneration due to sleep-disordered breathing exacerbates pathology in a mouse model of Alzheimer's disease Nature communications 2022-11-02 [PMID: 36323689] (IHC-FrFl, Mouse)
• Yerlikaya EI, Toro AL, Sunilkumar S et Al. Spleen Tyrosine Kinase Contributes to Müller Glial Expression of Proangiogenic Cytokines in Diabetes Invest Ophthalmol Vis Sci 2022-10-28 [PMID: 36306144] (Western Blot)
• Yin L, Zhang J, Sun Y Early growth response-1 is a new substrate of the GSK3beta-FBXW7 axis Neoplasia (New York, N.Y.) 2022-10-11 [PMID: 36240645]
• Martinez CA, Jiramongkol Y, Bal N et al. Intermittent hypoxia enhances the expression of hypoxia inducible factor HIF1A through histone demethylation Journal of Biological Chemistry 2022-11-01 [PMID: 36174675] (Western Blot, Mouse)
• Xun Wang, Yuemeng Jia, Jiawei Zhao, Nicholas P. Lesner, Cameron J. Menezes, Spencer D. Shelton, Siva Sai Krishna Venigalla, Jian Xu, Chunyu Cai, Prashant Mishra A mitofusin 2/HIF1 alpha axis sets a maturation checkpoint in regenerating skeletal muscle The Journal of Clinical Investigation 2022-12-01 [PMID: 36125902]
• Depanwita Saha, Debarpan Mitra, Neyaz Alam, Sagar Sen, Saunak Mitra Mustafi, Pradip K Majumder, Biswanath Majumder, Nabendu Murmu Lupeol and Paclitaxel cooperate in hindering hypoxia induced vasculogenic mimicry via suppression of HIF-1 alpha -EphA2-Laminin-5 gamma 2 network in human oral cancer. Journal of cell communication and signaling 2023-09-01 [PMID: 36063341]
• Soler-Agesta R, Marco-Brualla J, MinjArez-SAenz M et al. PT-112 Induces Mitochondrial Stress and Immunogenic Cell Death, Targeting Tumor Cells with Mitochondrial Deficiencies Cancers 2022-08-09 [PMID: 36010843] (WB, Mouse)
• Simmler P, Cortijo C, Koch LM et al. SF3B1 facilitates HIF1-signaling and promotes malignancy in pancreatic cancer Cell reports 2022-08-23 [PMID: 36001976] (WB, Chemotaxis)
• Chen J, Tang TT, Cao JY et al. KIM-1 augments hypoxia-induced tubulointerstitial inflammation through uptake of small extracellular vesicles by tubular epithelial cells Molecular therapy : the journal of the American Society of Gene Therapy 2022-08-18 [PMID: 35982620] (IHC-P, Mouse)
• Chang YH, Vuong CK, Ngo NH et al. Extracellular vesicles derived from Wharton's Jelly mesenchymal stem cells inhibit the tumor environment via the miR-125b/HIF1 alpha signaling pathway Scientific reports 2022-08-08 [PMID: 35941273] (WB, Human)
• Wang H, Wang X, Ma L et al. PGC-1 alpha regulates mitochondrial biogenesis to ameliorate hypoxia-inhibited cementoblast mineralization Annals of the New York Academy of Sciences 2022-08-02 [PMID: 35917205]
• Li G, Li D, Wu C et al. Homocysteine-targeting compounds as a new treatment strategy for diabetic wounds via inhibition of the histone methyltransferase SET7/9 Experimental & molecular medicine 2022-07-20 [PMID: 35859119] (IP, Human)
• Yao F, Chu M, Xi G et al. Single-Embryo Transcriptomic Atlas of Oxygen Response Reveals the Critical Role of HIF-1 alpha in Prompting Major Zygotic Gene Activation SSRN Electronic Journal 2022-02-24
• Takiyama T, Sera T, Nakamura M et al. A maternal high-fat diet induces fetal origins of NASH-HCC in mice Scientific reports 2022-07-30 [PMID: 35907977] (IHC-P, Mouse)
• Ollonen T, Kurkela M, Laitakari A et al. Activation of the hypoxia response protects mice from amyloid-beta accumulation Cellular and molecular life sciences : CMLS 2022-07-19 [PMID: 35852609] (WB, Mouse)
  
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  1:5000 Dilution
• Inna Gertsenshteyn, Boris Epel, Amandeep Ahluwalia, Heejong Kim, Xiaobing Fan, Eugene Barth, Marta Zamora, Erica Markiewicz, Hsiu-Ming Tsai, Subramanian Sundramoorthy, Lara Leoni, John Lukens, Mohammed Bhuiyan, Richard Freifelder, Anna Kucharski, Mihai Giurcanu, Brian B Roman, Gregory Karczmar, Chien-Min Kao, Howard Halpern, Chin-Tu Chen The optimal 18 F-fluoromisonidazole PET threshold to define tumor hypoxia in preclinical squamous cell carcinomas using pO 2 electron paramagnetic resonance imaging as reference truth. European journal of nuclear medicine and molecular imaging 2022-10-04 [PMID: 35792927]
• Tapio J, Halmetoja R, Dimova E et al. Contribution of HIF-P4H isoenzyme inhibition to metabolism indicates major beneficial effects being conveyed by HIF-P4H-2 antagonism Journal of Biological Chemistry 2022-07-01 [PMID: 35787374] (WB, Mouse)
• Chen M, Hu R, Cavinato C et al. Fibronectin-integrin alpha 5 signaling in vascular complications of type 1 diabetes Diabetes 2022-06-30 [PMID: 35771994]
• Li S, Li W, Yuan J et al. Impaired oxygen-sensitive regulation of mitochondrial biogenesis within the von Hippel-Lindau syndrome Nature metabolism 2022-06-01 [PMID: 35760869] (WB, Human)
• Xiaoyu Tang, Kaixuan Cui, Xi Lu, Peiqi Wu, Shanshan Yu, Boyu Yang, Yue Xu, Xiaoling Liang A Novel Hypoxia-inducible Factor 1 alpha Inhibitor KC7F2 Attenuates Oxygen-induced Retinal Neovascularization Investigative Ophthalmology & Visual Science 2022-06-13 [PMID: 35695808]
• Zhang W, Gong P, Tian Q et al. The eIF4A Inhibitor Silvestrol Blocks the Growth of Human Glioblastoma Cells by Inhibiting AKT/mTOR and ERK1/2 Signaling Pathway Journal of Oncology 2022-05-30 [PMID: 35677890] (Western Blot, Mouse)
• Han JS, Jeon YG, Oh M et al. Adipocyte HIF2 alpha functions as a thermostat via PKA C alpha regulation in beige adipocytes Nature communications 2022-06-07 [PMID: 35672324] (Western Blot, Mouse)
• Mirchandani AS, Jenkins SJ, Bain CC et al. Hypoxia shapes the immune landscape in lung injury and promotes the persistence of inflammation Nature Immunology 2022-06-01 [PMID: 35624205] (Western Blot, Mouse)
• Burda JE, O'Shea TM, Ao Y et al. Divergent transcriptional regulation of astrocyte reactivity across disorders Nature 2022-05-25 [PMID: 35614216] (IHC-Fr, Mouse)
• Zhang RM, Tiedemann K, Muthu ML et al. Fibrillin-1-regulated miR-122 has a critical role in thoracic aortic aneurysm formation Cellular and molecular life sciences : CMLS 2022-05-23 [PMID: 35606547]
• Baran N, Lodi A, Dhungana Y et al. Inhibition of mitochondrial complex I reverses NOTCH1-driven metabolic reprogramming in T-cell acute lymphoblastic leukemia Nature communications 2022-05-19 [PMID: 35589701] (CyTof, Human)
• Mart�nez-Torres I, Tepale-Segura A, Castro-Escamilla O et al. The Protective Role of pVHL in Imiquimod-Induced Psoriasis-like Skin Inflammation International Journal of Molecular Sciences 2022-05-07 [PMID: 35563616] (Western Blot, Mouse)
• S A F, K S C, L D et al. Engineering Vascularized Islet Macroencapsulation Devices: An in vitro Platform to Study Oxygen Transport in Perfused Immobilized Pancreatic Beta Cell Cultures Frontiers in Bioengineering and Biotechnology 2022-04-19 [PMID: 35519615] (Western Blot, Mouse)
• Salman S, Meyers DJ, Wicks EE Et al. HIF inhibitor 32-134D eradicates murine hepatocellular carcinoma in combination with anti-PD1 therapy J Clin Invest 2022-05-02 [PMID: 35499076] (WB, Chemotaxis, Mouse, Human)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Leigh T, Kawai T, Preston K et al. Deletion of LDLRAP1 Induces Atherosclerotic Plaque Formation, Insulin Resistance, and Dysregulated Insulin Response in Adipose Tissue The American journal of pathology 2022-04-20 [PMID: 35460615] (IF/IHC, Mouse)
• Wu X, Chen J, Liu C et al. Slc38a9 Deficiency Induces Apoptosis and Metabolic Dysregulation and Leads to Premature Death in Zebrafish International journal of molecular sciences 2022-04-11 [PMID: 35457018] (IP, Zebrafish)
• Xu ZH, Wang C, He YX et al. Hypoxia-inducible factor protects against acute kidney injury via the Wnt/beta-catenin signaling pathway American journal of physiology. Renal physiology [PMID: 35403451]
• Kirschner K, Kelterborn S, Stehr H et al. Adaptation of the Oxygen Sensing System during Lung Development Oxidative Medicine and Cellular Longevity 2022-02-18 [PMID: 35242281] (WB, Mouse)
• Jatho A, Zieseniss A, Brechtel-Curth K et al. The HIF alpha-Stabilizing Drug Roxadustat Increases the Number of Renal Epo-Producing Sca-1+ Cells Cells 2022-02-21 [PMID: 35203399] (WB)
• Zheng X, Narayanan S, Xu C et al. Repression of hypoxia-inducible factor-1 contributes to increased mitochondrial reactive oxygen species production in diabetes eLife 2022-02-15 [PMID: 35164902] (Western Blot, Mouse)
• Snyder B, Wu HK, Tillman B, Floyd TF Aged Mouse Hippocampus Exhibits Signs of Chronic Hypoxia and an Impaired HIF-Controlled Response to Acute Hypoxic Exposures Cells 2022-01-26 [PMID: 35159233] (IHC-Fr, Mouse)
• Yang Y, Lu H, Chen C et al. HIF-1 Interacts with TRIM28 and DNA-PK to release paused RNA polymerase II and activate target gene transcription in response to hypoxia Nature communications 2022-01-14 [PMID: 35031618] (Chemotaxis)
• Krzywinska E, Sobecki M, Nagarajan S et al. The transcription factor HIF-1? mediates plasticity of NKp46+ innate lymphoid cells in the gut Journal of Experimental Medicine 2022-02-07 [PMID: 35024767] (Western Blot, Mouse)
• Elamaa H, Kaakinen M, NAtynki M et al. PHD2 deletion in endothelial or arterial smooth muscle cells reveals vascular cell type-specific responses in pulmonary hypertension and fibrosis Angiogenesis 2022-01-08 [PMID: 34997404] (WB, Mouse)
• Michon S, Rodier F, Yu FTH Targeted Anti-Cancer Provascular Therapy Using Ultrasound, Microbubbles, and Nitrite to Increase Radiotherapy Efficacy Bioconjugate chemistry 2022-01-06 [PMID: 34990112]
• ROning T, Magga J, Laitakari A et al. Activation of the hypoxia response pathway protects against age-induced cardiac hypertrophy Journal of molecular and cellular cardiology 2021-12-14 [PMID: 34919895] (WB, Mouse)
• Li Y, Wang C, Xu T et Al. Discovery of a small molecule inhibitor of cullin neddylation that triggers ER stress to induce autophagy Acta Pharm Sin B 2021-07-17 [PMID: 34900537]
• Te Boekhorst V, Jiang L, MAhlen M et al. Calpain-2 regulates hypoxia/HIF-induced plasticity toward amoeboid cancer cell migration and metastasis Current biology : CB 2021-12-06 [PMID: 34883047]
• Ali FF, Mohammed HH, Elroby Ali DM Protective effect of hydrogen sulfide against stress-induced lung injury: involvement of Nrf2, NF kappa B/iNOS, and HIF-1 alpha signaling pathways Cell stress & chaperones 2021-12-08 [PMID: 34881408]
• Borella R, De Biasi S, Paolini A et al. Metabolic reprograming shapes neutrophil functions in severe COVID-19 European journal of immunology 2021-12-06 [PMID: 34870329] (WB, Human)
• Suwa T, Kobayashi M, Shirai Y Et al. SPINK1 as a plasma marker for tumor hypoxia and a therapeutic target for radiosensitization JCI insight 2021-11-08 [PMID: 34747365] (IHC-P, Human)
• Wang J, Zhang H, Kaul A Et al. Effect of Ashwagandha Withanolides on Muscle Cell Differentiation Biomolecules 2021-10-04 [PMID: 34680087] (WB, ICC/IF, Mouse)
• Angeles CV, Velez A, Rios J et al. A High-Content Screen for C/EBP? Expression Identifies Novel Therapeutic Agents in Dedifferentiated Liposarcoma Clinical Cancer Research 2022-01-01 [PMID: 34667024] (Western Blot, Mouse)
• Foglia B, Sutti S, Cannito S Et al. Hepatocyte-Specific Deletion of HIF2 alpha Prevents NASH-Related Liver Carcinogenesis by Decreasing Cancer Cell Proliferation Cellular and molecular gastroenterology and hepatology 2021-10-14 [PMID: 34655812]
• Kumar A, Vaish M, Karuppagounder SS et al. HIF1? stabilization in hypoxia is not oxidant-initiated eLife 2021-10-01 [PMID: 34596045] (Western Blot, Mouse)
• Haiquan Lu, Yajing Lyu, Linh Tran, Jie Lan, Yangyiran Xie, Yongkang Yang, Naveena L Murugan, Yueyang J Wang, Gregg L Semenza HIF-1 recruits NANOG as a coactivator for TERT gene transcription in hypoxic breast cancer stem cells. Cell reports 2022-02-10 [PMID: 34592152] (Western Blot)
• Pang L, Ng KT, Liu J et al. Plasmacytoid dendritic cells recruited by HIF-1 alpha /eADO/ADORA1 signaling induce immunosuppression in hepatocellular carcinoma Cancer letters 2021-09-16 [PMID: 34536555] (Chemotaxis, Human)
• Wakefield ZR, Tanaka M, Pampo C et al. Normal tissue and tumor microenvironment adaptations to aerobic exercise enhance doxorubicin anti-tumor efficacy and ameliorate its cardiotoxicity in retired breeder mice Oncotarget 2021-08-31 [PMID: 34504647] (Western Blot, Mouse)
• Guangxi Sun, Junru Chen, Jiayu Liang, Xiaoxue Yin, Mengni Zhang, Jin Yao, Ning He, Cameron M. Armstrong, Linmao Zheng, Xingming Zhang, Sha Zhu, Xiaomeng Sun, Xiaoxia Yang, Wanbin Zhao, Banghua Liao, Xiuyi Pan, Ling Nie, Ling Yang, Yuntian Chen, Jinge Zhao, Haoran Zhang, Jindong Dai, Yali Shen, Jiyan Liu, Rui Huang, Jiandong Liu, Zhipeng Wang, Yuchao Ni, Qiang Wei, Xiang Li, Qiao Zhou, Haojie Huang, Zhenhua Liu, Pengfei Shen, Ni Chen, Hao Zeng Integrated exome and RNA sequencing of TFE3 -translocation renal cell carcinoma Nature Communications 2021-09-06 [PMID: 34489456]
• Sun D, Lu F, Sheldon A et al. Neuronal deficiency of hypoxia-inducible factor 2? increases hypoxic-ischemic brain injury in neonatal mice Journal of Neuroscience Research 2021-11-01 [PMID: 34487578] (Western Blot, Mouse)
• Jeny F, Bernaudin JF, Valeyre D Et al. Hypoxia Promotes a Mixed Inflammatory-Fibrotic Macrophages Phenotype in Active Sarcoidosis Frontiers in immunology 2021-08-11 [PMID: 34456926] (ICC/IF, Human)
• Helena J, Joubert A, Mabeta P Et al. Intracellular Signaling Responses Induced by Radiation within an In Vitro Bone Metastasis Model after Pre-Treatment with an Estrone Analogue Cells 2021-08-17 [PMID: 34440874] (WB, Human, Mouse)
• Wang J, Liu R, Wang Y Et Al. Repression of the miR-627-5p by histone deacetylase 3 contributes to hypoxia-induced hepatocellular carcinoma progression J Cancer 2021-08-02 [PMID: 34335948] (Chemotaxis)
• Nanduri J, Wang N, Wang Bl, Prabhakar Nr Lysine demethylase KDM6B regulates HIF-1 alpha mediated systemic and cellular responses to intermittent hypoxia Physiological genomics 2021-07-23 [PMID: 34297635]
• Ju S, Lim L, Wi K et al. LRP5 Regulates HIF-1 alpha Stability via Interaction with PHD2 in Ischemic Myocardium International Journal of Molecular Sciences 2021-06-19 [PMID: 34205318] (IP, Rat)
• Williams AL, Walton CB, Pinell B et al. Ischemic heart injury leads to HIF1-dependent differential splicing of CaMK2 gamma Scientific reports 2021-06-23 [PMID: 34162925] (Mouse)
• Simmen S, Maane M, Rogler S et al. Hypoxia Reduces the Transcription of Fibrotic Markers in the Intestinal Mucosa Inflammatory Intestinal Diseases 2021-03-29 [PMID: 34124180]
• Grubman A, Choo XY, Chew G et al. Transcriptional signature in microglia associated with A beta plaque phagocytosis Nature communications 2021-05-21 [PMID: 34021136] (IF/IHC, Mouse)
• Bae WY, Choi JS, Nam S, Jeong JW beta-arrestin 2 stimulates degradation of HIF-1 alpha and modulates tumor progression of glioblastoma Cell death and differentiation 2021-05-18 [PMID: 34007068]
• Silwal P, Kim JK, Jeon SM et al. Mitofusin-2 boosts innate immunity through the maintenance of aerobic glycolysis and activation of xenophagy in mice Communications biology 2021-05-10 [PMID: 33972668]
• Wang R, Godet I, Yang Y, et al. Hypoxia-inducible factor-dependent ADAM12 expression mediates breast cancer invasion and metastasis Proc Natl Acad Sci 2021-05-11 [PMID: 33952697] (Chemotaxis, WB)
• Kuwano A, Kurokawa M, Kohjima M et al. Microcirculatory disturbance in acute liver injury Experimental and therapeutic medicine 2021-06-01 [PMID: 33884034] (Western Blot, Mouse)
• Ertle CM, Rommel FR, Tumala S et al. New Pathways for the Skin's Stress Response: The Cholinergic Neuropeptide SLURP-1 Can Activate Mast Cells and Alter Cytokine Production in Mice Frontiers in Immunology 2021-03-18 [PMID: 33815383] (Western Blot, Mouse)
• Pantazopoulou V, Jeannot P, Rosberg R et al. Hypoxia-Induced Reactivity of Tumor-Associated Astrocytes Affects Glioma Cell Properties Cells 2021-03-10 [PMID: 33802060] (WB)
• Yano, M, Miyazawa, M Et al. Up-regulation of HDAC6 Results in Poor Prognosis and Chemoresistance in Patients With Advanced Ovarian High-grade Serous Carcinoma. Anticancer Res 2021-03-01 [PMID: 33788761]
• Hutami IR, Izawa T, Khurel-Ochir T et al. HIF-1 alpha controls palatal wound healing by regulating macrophage motility via S1P/S1P1 signaling axis Oral diseases 2021-03-23 [PMID: 33759275]
• Khuu MA, Nallamothu T, Castro-Rivera CI et al. Stage-dependent effects of intermittent hypoxia influence the outcome of hippocampal adult neurogenesis Scientific reports 2021-03-16 [PMID: 33727588] (IHC-Fr, Mouse)
• Ivan Menendez-Montes, Beatriz Escobar, Manuel J. Gomez, Teresa Albendea-Gomez, Beatriz Palacios, Elena Bonzon-Kulichenko, Jose Luis Izquierdo-Garcia, Ana Vanessa Alonso, Alessia Ferrarini, Luis Jesus Jimenez-Borreguero, Jesus Ruiz-Cabello, Jesus Vázquez, Silvia Martin-Puig Activation of amino acid metabolic program in cardiac HIF1-alpha-deficient mice iScience 2021-02-03 [PMID: 33665549]
• Choi YW, Kim YH, Oh SY et al. Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer Advanced Science 2021-01-04 [PMID: 33643790] (Western Blot, Mouse)
• Akyuz E, Doganyigit Z, Eroglu E et al. Myocardial Iron Overload in an Experimental Model of Sudden Unexpected Death in Epilepsy Frontiers in Neurology 2021-02-11 [PMID: 33643194] (IHC-P, Rat)
• Plastino F, Santana-Garrido A, Pesce NA et al. Echinomycin mitigates ocular angiogenesis by transcriptional inhibition of the hypoxia-inducible factor-1 Experimental eye research 2021-02-24 [PMID: 33639134] (WB, Mouse)
• Tanimura A, Nakazato A, Tanaka N MYD88 signals induce tumour-initiating cell generation through the NF-kappa B-HIF-1 alpha activation cascade Scientific reports 2021-02-17 [PMID: 33597599] (WB, Mouse)
• Chung YJ, Swietach P, Curtis MK et al. Iron-Deficiency Anemia Results in Transcriptional and Metabolic Remodeling in the Heart Toward a Glycolytic Phenotype Frontiers in Cardiovascular Medicine 2021-01-21 [PMID: 33553263] (Western Blot, Mouse)
• Pesce NA, Canovai A, Lardner E, et al. Autophagy Involvement in the Postnatal Development of the Rat Retina Cells 2021-01-17 [PMID: 33477313] (WB, Rat)
• Goggins BJ, Minahan K, Sherwin S, et al. Pharmacological HIF-1 stabilization promotes intestinal epithelial healing through regulation of alpha -integrin expression and function American journal of physiology. Gastrointestinal and liver physiology 2021-01-20 [PMID: 33470153] (Chemotaxis, Human)
• Markolin P, Davidson N, Hirt CK, et al. Identification of HIF-dependent alternative splicing in gastrointestinal cancers and characterization of a long, coding isoform of SLC35A3 Genomics 2021-01-05 [PMID: 33418078] (WB, Human)
• Tran CW, Gold MJ, Garcia-Batres C, et al. Hypoxia-inducible factor 1 alpha limits dendritic cell stimulation of CD8 T cell immunity PloS one 2020-12-31 [PMID: 33382742] (WB, Mouse)
• Pelaz SG, JaraIz-RodrIguez M, Alvarez-VAzquez A et al. Targeting metabolic plasticity in glioma stem cells in vitro and in vivo through specific inhibition of c-Src by TAT-Cx43266-283 EBioMedicine 2020-11-27 [PMID: 33254027] (WB)
• Almeida L, Dhillon-LaBrooy A, Castro CN et al. Ribosome-Targeting Antibiotics Impair T Cell Effector Function and Ameliorate Autoimmunity by Blocking Mitochondrial Protein Synthesis Immunity 2020-11-17 [PMID: 33238133] (WB, Mouse)
  
  Details:
  Naïve T cells from the lymph nodes and spleens of C57BL/6 mice, whose Gfm1 gene had been deleted, underwent Western blot analysis.
• GOmez-Lado N, LOpez-Arias E, Iglesias-Rey R et al. [18F]-FMISO PET/MRI Imaging Shows Ischemic Tissue around Hematoma in Intracerebral Hemorrhage Mol Pharm 2020-11-13 [PMID: 33186043] (Human)
• Grassi E, Jeannot P, Pantazopoulou V et al. Niche-derived soluble DLK1 promotes glioma growth Neoplasia 2020-12-01 [PMID: 33142235] (Western Blot, Mouse)
• Zhang YH, Yan XZ, Xu SF et al. ?-Lipoic Acid Maintains Brain Glucose Metabolism via BDNF/TrkB/HIF-1? Signaling Pathway in P301S Mice Frontiers in Aging Neuroscience 2020-08-21 [PMID: 32973490] (Western Blot, Mouse)
• Yang, X, Zhong, D Et al. Conditional ablation of MAPK7 expression in chondrocytes impairs endochondral bone formation in limbs and adaptation of chondrocytes to hypoxia. Cell Biosci 2020-09-19 [PMID: 32944217] (IF/IHC, WB, Mouse)
• Usha Patel, Manish Pandey, Sadhana Kannan, Tanuja A. Samant, Poonam Gera, Neha Mittal, Swapnil Rane, Asawari Patil, Vanita Noronha, Amit Joshi, Vijay M. Patil, Kumar Prabhash, Manoj B. Mahimkar Prognostic and predictive significance of nuclear HIF1 alpha expression in locally advanced HNSCC patients treated with chemoradiation with or without nimotuzumab British Journal of Cancer 2020-09-17 [PMID: 32939054]
• Wang N, Shi XF, Khan SA et al. Hypoxia inducible factor-1 mediates pancreatic beta-cell dysfunction by intermittent hypoxia Am. J. Physiol., Cell Physiol. 2020-09-16 [PMID: 32936698] (ICC/IF, Mouse)
• Kim, C S, Ding, X Et al. Glutamine Metabolism Controls Stem Cell Fate Reversibility and Long-Term Maintenance in the Hair Follicle. Cell Metab 2020-10-06 [PMID: 32905798] (WB, Mouse)
• Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (Chemotaxis, WB, Mouse)
  
  Details:
  Citation using the PE format of this antibody.
• Thomas A, Samykutty A, Gomez-Gutierrez JG et al. Actively Targeted Nanodelivery of Echinomycin Induces Autophagy-Mediated Death in Chemoresistant Pancreatic Cancer In Vivo Cancers (Basel) 2020-08-14 [PMID: 32823919] (WB, Human)
• Huang BR, Liu YS, Lai SW et al. CAIX Regulates GBM Motility and TAM Adhesion and Polarization through EGFR/STAT3 under Hypoxic Conditions International Journal of Molecular Sciences 2020-08-14 [PMID: 32823915] (Western Blot, Mouse)
• Babetto E, Wong KM, Beirowski B A glycolytic shift in Schwann cells supports injured axons Nat. Neurosci. 2020-08-17 [PMID: 32807950] (ICC/IF, Mouse)
• Hoefflin R, Harlander S, SchAfer S et al. HIF-1 alpha and HIF-2 alpha differently regulate tumour development and inflammation of clear cell renal cell carcinoma in mice Nat Commun 2020-01-01 [PMID: 32807776] (WB, Mouse)
• Tiwari A, Tashiro K, Dixit A Et Al. Loss of HIF1A From Pancreatic Cancer Cells Increases Expression of PPP1R1B and Degradation of p53 to Promote Invasion and Metastasis Gastroenterology 2020-08-03 [PMID: 32768595] (Mouse)
• Ye Y, Chen Y, Sun J et al. Hyperglycemia suppresses the regulatory effect of hypoxia-inducible factor-1 alpha in pulmonary Aspergillus fumigatus infection Pathog Dis 2020-07-01 [PMID: 32678442] (WB, Mouse)
• Ma J, Stefanoska D, Stone L et al. Hypoxic stress enhances extension and branching of dorsal root ganglion neuronal outgrowth JOR Spine 2020-05-04 [PMID: 32613165] (ICC/IF, Rat, Mouse)
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  Details:
  HIF1 alpha antibody used to show the effect on cells of YC-1 exposure in both normoxic and hypoxic by western blot in mouseNIH/3T3 cells
• Sharma B, Ho L, Ford GH et al. Alternative Progenitor Cells Compensate to Rebuild the Coronary Vasculature in Elabela- and Apj-Deficient Hearts Dev. Cell 2017-09-25 [PMID: 28890073] (IF/IHC, Mouse)
• Huang R, Hayashi Y, Yan X et al HIF1A is a critical downstream mediator for hemophagocytic lymphohistiocytosis. Haematologica. 2017-11-01 [PMID: 28860338] (Mouse)
  
  Details:
  Citation using the DyLight 405 version of this antibody.
• Johansson E, Grassi ES, Pantazopoulou V et al. CD44 Interacts with HIF-2alpha to Modulate the Hypoxic Phenotype of Perinecrotic and Perivascular Glioma Cells Cell Rep 2017-08-15 [PMID: 28813675] (WB, Human)
• Sun W, Kato H, Kitajima S et al. Interaction between von Hippel-Lindau Protein and Fatty Acid Synthase Modulates Hypoxia Target Gene Expression. Sci Rep 2017-08-03 [PMID: 28775317] (WB, Human)
• Nakashima R, Goto Y, Koyasu S et al. UCHL1-HIF-1 axis-mediated antioxidant property of cancer cells as a therapeutic target for radiosensitization Sci Rep 2017-07-31 [PMID: 28761052] (WB, Human)
• Braverman J, Stanley SA. Nitric Oxide Modulates Macrophage Responses to Mycobacterium tuberculosis Infection through Activation of HIF-1alpha and Repression of NF-kB J. Immunol. 2017-07-28 [PMID: 28754681] (WB)
• Cosin-Roger J, Simmen S, Melhem H et al. Hypoxia ameliorates intestinal inflammation through NLRP3/mTOR downregulation and autophagy activation Nat Commun 2017-07-24 [PMID: 28740109] (WB, Human)
• Casciello F, Al-Ejeh F, Kelly G et al. G9a drives hypoxia-mediated gene repression for breast cancer cell survival and tumorigenesis. Proc. Natl. Acad. Sci. U.S.A. 2017-06-19 [PMID: 28630300] (WB, Human)
• Zheng X, Narayanan S, Zheng X et al. A Notch-independent mechanism contributes to the induction of Hes1 gene expression in response to hypoxia in P19 cells. Exp. Cell Res. 2017-06-09 [PMID: 28602625] (WB, IP, Mouse)
• Yasui T, Uezono N, Nakashima H et al. Hypoxia Epigenetically Confers Astrocytic Differentiation Potential on Human Pluripotent Cell-Derived Neural Precursor Cells. Stem Cell Reports. 2017-06-06 [PMID: 28591654]
• Licarete E, Sesarman A, Rauca VF et al. HIF-1a acts as a molecular target for simvastatin cytotoxicity in B16.F10 melanoma cells cultured under chemically induced hypoxia. Oncol Lett 2017-05-01 [PMID: 28521491] (WB, Mouse)
• Kiriakidis S, Hoer SS, Burrows N et al. Complement C1q is hydroxylated by collagen prolyl 4 hydroxylase and is sensitive to off-target inhibition by prolyl hydroxylase domain inhibitors that stabilize hypoxia-inducible factor. Kidney Int. 2017-05-12 [PMID: 28506759] (IB, Mouse)
• Jackson CS, Durandt C, Janse van Rensburg I et al. Targeting the aryl hydrocarbon receptor nuclear translocator complex with DMOG and Stemregenin 1 improves primitive hematopoietic stem cell expansion. Stem Cell Res. 2017-04-20 [PMID: 28445828]
• Lin Yu-Jung, Shyu Woei-Cherng, Chang Chi-Wei et al. Tumor Hypoxia Regulates Forkhead Box C1 to Promote Lung Cancer Progression. Theranostics 2017-03-05 [PMID: 28435457] (Chemotaxis, IHC-Fr, WB, Human)
• Tokumoto Y, Tamaki S, Kabe Y et al. Quiescence of adult oligodendrocyte precursor cells requires thyroid hormone and hypoxia to activate Runx1. Sci Rep 2017-04-21 [PMID: 28432293] (ICC/IF, Rat)
• Duan C, Perez-Torres CJ, Yuan L et al. Can anti-vascular endothelial growth factor antibody reverse radiation necrosis. A preclinical investigation. J. Neurooncol. 2017-04-19 [PMID: 28425047]
• Schuoler C, Haider TJ, Leuenberger C et al. Aquaporin 1 controls the functional phenotype of pulmonary smooth muscle cells in hypoxia-induced pulmonary hypertension. Basic Res. Cardiol. 2017-05-01 [PMID: 28409279] (WB, Mouse)
• Hum JM, Clinkenbeard EL, Ip C et al. The metabolic bone disease associated with the Hyp mutation is independent of osteoblastic HIF1a expression. Bone Reports. 2017-01-17 [PMID: 28377980] (WB, Rat)
• Sato Y, Tsuyama T, Sato C et al. Hypoxia reduces HNF4alpha/MODY1 protein expression in pancreatic B-cells by activating AMP-activated protein kinase. J. Biol. Chem. 2017-03-31 [PMID: 28364040] (WB, Mouse)
• Sourabh S, Padwad Y HIF-1 in cancer therapy: two decade long story of a transcription factor. Acta Oncol 2017-01-04 [PMID: 28358664] (IF/IHC, Human)
• Landfried B, Samardzija M, Barben M et al. Digoxin-induced retinal degeneration depends on rhodopsin. Cell Death Dis. 2017-03-16 [PMID: 28300845] (WB, Mouse)
• Wang S, Zeng H, Chen ST et al. Ablation of endothelial prolyl hydroxylase domain protein-2 promotes renal vascular remodelling and fibrosis in mice. J. Cell. Mol. Med. 2017-03-07 [PMID: 28266128] (WB, Mouse)
• Maturu P, Jones D, Ruteshouser EC et al. Role of Cyclooxygenase-2 Pathway in Creating an Immunosuppressive Microenvironment and in Initiation and Progression of Wilms' Tumor. Neoplasia. 2017-03-01 [PMID: 28254151] (IHC-P, Mouse)
• Asai, Y, Yamada, T Et al. Activation of the Hypoxia Inducible Factor 1 alpha Subunit Pathway in Steatotic Liver Contributes to Formation of Cholesterol Gallstones. Gastroenterology 2017-05-01 [PMID: 28088462] (WB, Human)
• Brock M, Schuoler C, Leuenberger C et al. Analysis of hypoxia-induced noncoding RNAs reveals metastasis-associated lung adenocarcinoma transcript 1 as an important regulator of vascular smooth muscle cell proliferation Exp. Biol. Med. (Maywood) 2017-01-01 [PMID: 28056547] (Mouse)
• Razafinjatovo CF, Stiehl D, Deininger E et al. VHL missense mutations in the p53 binding domain show different effects on p53 signaling and HIFalpha degradation in clear cell renal cell carcinoma. Oncotarget. 2017-02-07 [PMID: 28052007] (WB, Mouse)
• Negrette-Guzman M, Huerta-Yepez S, Vega MI et al. Sulforaphane induces differential modulation of mitochondrial biogenesis and dynamics in normal cells and tumor cells. Food Chem. Toxicol. 2016-12-18 [PMID: 27993529] (ICC/IF, Human)
• Myllymaki MN, Maatta J, Dimova EY et al. Notch downregulation and extramedullary erythrocytosis in HIF prolyl 4-hydroxylase-2-deficient mice. Mol. Cell. Biol. 2016-11-07 [PMID: 27821476]
• Morita M, Sato Y, Iwasaki R et al. Selective Estrogen Receptor Modulators Suppress Hif1alpha Protein Accumulation in Mouse Osteoclasts. PLoS ONE. 2016-11-01 [PMID: 27802325] (WB, Moth - Trichoplusia ni (Cabbage Looper))
• Hoogewijs D, Vogler M, Zwenger E et al. Oxygen-dependent regulation of aquaporin-3 expression. Hypoxia (Auckl). 2016-11-01 [PMID: 27800511] (Mouse)
• Guentsch A, Beneke A, Swain L et al. PHD2 Is a Regulator for Glycolytic Reprogramming in Macrophages. Mol. Cell. Biol. 2017-01-01 [PMID: 27795296]
• Tian X, Nguyen M, Foote HP et al. CRLX101, a nanoparticle-drug conjugate containing camptothecin, improves rectal cancer chemoradiotherapy by inhibiting DNA repair and HIF-1alpha. Cancer Res. 2016-10-26 [PMID: 27784746]
• Ostergaard L, Rudiger A, Wellmann S et al. Arginine-vasopressin marker copeptin is a sensitive plasma surrogate of hypoxic exposure. Hypoxia (Auckl) 2014-09-11 [PMID: 27774473]
• Sousa LM, Silva RD, Fonseca VU et al. Is the canine corpus luteum an insulin-sensitive tissue?. J. Endocrinol. 2016-12-01 [PMID: 27679426]
• Clever D, Roychoudhuri R, Constantinides MG et al. Oxygen Sensing by T Cells Establishes an Immunologically Tolerant Metastatic Niche. Cell. 2016-08-25 [PMID: 27565342]
• Koivunen P, Fell SM, Lu W et al. The 2-oxoglutarate analog 3-oxoglutarate decreases normoxic hypoxia-inducible factor-1alpha in cancer cells, induces cell death, and reduces tumor xenograft growth. Hypoxia (Auckl) 2016-03-04 [PMID: 27525289]
• Pandya PH, Wilkes DS. HIF-1alpha regulates CD55 expression in airway epithelium. Am J Respir Cell Mol Biol 2016-08-06 [PMID: 27494303]
• Guimaraes TA, Farias LC, Santos ES et al. Metformin increases PDH and suppresses HIF-1alpha under hypoxic conditions and induces cell death in oral squamous cell carcinoma. Oncotarget. 2016-07-26 [PMID: 27474170] (WB, IF/IHC, Human)
• Jung GS, Jeon JH, Choi YK et al. Pyruvate dehydrogenase kinase regulates hepatitis C virus replication. Sci Rep. 2016-07-29 [PMID: 27471054] (WB, IF/IHC, Human)
• Leinonen H, Rossi M, Salo AM et al. Lack of P4H-TM in mice results in age-related retinal and renal alterations. Hum. Mol. Genet. 2016-07-27 [PMID: 27466183] (WB, Mouse)
• Xue H, Chen D, Zhong YK et al. Deferoxamine ameliorates hepatosteatosis via several mechanisms in ob/ob mice Ann. N. Y. Acad. Sci. 2016-07-01 [PMID: 27447538] (WB, Mouse)
• Aarup A, Pedersen T X et al Hypoxia-Inducible Factor-1 alpha Expression in Macrophages Promotes Development of Atherosclerosis Arterioscler Thromb Vasc Biol 2016-07-23 [PMID: 27444197] (IF/IHC, Mouse)
  
  Details:
  Citation using the Biotin version of this antibody.
• Braverman J, Sogi KM, Benjamin D et al. HIF-1 alpha Is an Essential Mediator of IFN-gamma-Dependent Immunity to Mycobacterium tuberculosis J. Immunol. 2016-07-18 [PMID: 27430718]
• Robl B, Botter SM, Pellegrini G et al. Evaluation of intraarterial and intravenous cisplatin chemotherapy in the treatment of metastatic osteosarcoma using an orthotopic xenograft mouse model J Exp Clin Cancer Res. 2016-07-15 [PMID: 27421768] (IHC-P, Mouse)
• Luo B, Wang J, Liu Z et al. Phagocyte respiratory burst activates macrophage erythropoietin signalling to promote acute inflammation resolution. Nat Commun. [PMID: 27397585] (WB)
  
  Details:
  Citation using the HRP form of this antibody.
• Nunomiya A, Shin J, Kitajima Y et al. Activation of the hypoxia-inducible factor pathway induced by prolyl hydroxylase domain 2 deficiency enhances the effect of running training in mice. Acta Physiol (Oxf) 2016-07-09 [PMID: 27393382] (WB, Mouse)
• Diaz-Trelles R, Scimia MC, Bushway P et al. Notch-independent RBPJ controls angiogenesis in the adult heart. Nat Commun 2016-06-30 [PMID: 27357444] (WB)
• Muinos-Lopez E, Ripalda-Cemborain P, Lopez-Martinez T et al. Hypoxia and Reactive Oxygen Species Homeostasis in Mesenchymal Progenitor Cells Define a Molecular Mechanism for Fracture Nonunion. Stem Cells 2016-06-02 [PMID: 27250101]
• Bullen JW, Tchernyshyov I, Holewinski RJ et al. Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1 Sci Signal 2016-05-31 [PMID: 27245613] (WB, Human)
• Halt KJ, Parssinen HE, Junttila SM, Saarela U. CD146+ cells are essential for kidney vasculature development. Kidney International. 2016-04-18 [PMID: 27165833] (WB, Mouse)
• Christinat Y, Pawlowski R, Krek W. jSplice: a high-performance method for accurate prediction of alternative splicing events and its application to large-scale renal cancer transcriptome data. Bioinformatics 2016-07-15 [PMID: 27153587]
• Ruffenach G, Chabot S, Tanguay VF et al. Role for RUNX2 in Proliferative and Calcified Vascular Lesions in Pulmonary Arterial Hypertension. Am. J. Respir. Crit. Care Med. 2016-05-05 [PMID: 27149112] (IHC-P, WB, IP, Human, Rat)
• Takahashi N, Davy PM, Gardner LH et al. Hypoxia Inducible Factor 1 Alpha Is Expressed in Germ Cells throughout the Murine Life Cycle. PLoS ONE. 2016-05-06 [PMID: 27148974] (ICC/IF, IHC-P, IHC-WhMt, Mouse)
• Msaki A, Pasto A, Curtarello M et al. A hypoxic signature marks tumors formed by disseminated tumor cells in the BALB-neuT mammary cancer model. Oncotarget. 2016-05-31 [PMID: 27105499] (WB, Mouse)
• Trinh NT, Yamashita T, Ohneda K et al. Increased Expression of EGR-1 in Diabetic Human Adipose Tissue-Derived Mesenchymal Stem Cells Reduces Their Wound Healing Capacity. Stem Cells Dev 2016-05-15 [PMID: 26988763]
• Shin J, Nunomiya A, Kitajima Y et al. Prolyl hydroxylase domain 2 deficiency promotes skeletal muscle fiber-type transition via a calcineurin/NFATc1-dependent pathway. Skelet Muscle 2016-03-05 [PMID: 26949511] (WB)
• Ruf M, Moch H, Schraml P. PD-L1 expression is regulated by hypoxia inducible factor in clear cell renal cell carcinoma. Int J Cancer 2016-07-15 [PMID: 26945902]
• Zhou F, Dai A, Jiang Y et al. SENP1 enhances hypoxiainduced proliferation of rat pulmonary artery smooth muscle cells by regulating hypoxiainducible factor1alpha. Mol Med Rep 2016-04-01 [PMID: 26935971] (WB)
• Rahtu-Korpela L, Maatta J et al. Hypoxia-Inducible Factor Prolyl 4-Hydroxylase-2 Inhibition Protects Against Development of Atherosclerosis. Arterioscler Thromb Vasc Biol 2016-01-04 [PMID: 26848160] (WB, Mouse)
• Regan Anderson TM, Ma SH, Raj GV et al. Breast Tumor Kinase (Brk/PTK6) Is Induced by HIF, Glucocorticoid Receptor, and PELP1-Mediated Stress Signaling in Triple-Negative Breast Cancer Cancer Res. 2016-01-29 [PMID: 26825173] (WB, Human)
• Lee SD, Kim W, Jeong JW et al. AK-1, a SIRT2 inhibitor, destabilizes HIF-1alpha and diminishes its transcriptional activity during hypoxia. Cancer Lett. 2016-01-22 [PMID: 26808575] (ICC/IF, Human)
  
  Details:
  HIF1 alpha antibody was used for immunofluorescence/ICC-IF staining of A549 cells which were exposed to hypoxia for six hours in the presence of a SIRT2-specific inhibitor (10uM AK-1 ).
• Qian F, He M, Duan W et al. Cross regulation between hypoxia-inducible transcription factor-1alpha (HIF-1alpha) and transforming growth factor (TGF)-ss1 mediates nickel oxide nanoparticles (NiONPs)-induced pulmonary fibrosis. Am J Transl Res 2015-01-01 [PMID: 26807184] (WB)
• Tando T, Sato Y, Miyamoto K et al. Hif1alpha is required for osteoclast activation and bone loss in male osteoporosis. Biochem. Biophys. Res. Commun. 2016-02-05 [PMID: 26792721] (IHC-P, WB, Mouse)
  
  Details:
  HIF1 alpha antibody was used for IHC-P analysis of femurs from orchidectomy/ORX or sham-operated Wild type C57BL/6 mice - the bones fixed 10% formalin, decalcified in 8% Na2EDTA, antigen was retrieved for 8 minutes at RT with 0.4 mg/mL Proteinase K and primary detected using Alexa488-conjugated goat anti-rabbit secondary (Fig. 1). The antibody was also used for WB on lysates of Raw264.7 cells cultured with or without testosterone in normoxic or hypoxic conditions.
• Kinoshita T, Fujii H, Hayashi Y et al. Prognostic significance of hypoxic PET using (18)F-FAZA and (62)Cu-ATSM in non-small-cell lung cancer. Lung Cancer 2016-01-01 [PMID: 26711935]
• Laukka T, Mariani CJ, Ihantola T et al. Fumarate and Succinate Regulate Expression of Hypoxia-inducible Genes via TET Enzymes. J Biol Chem 2016-02-19 [PMID: 26703470]
• Aquino-Galvez A, Gonzalez-avila G. Effects of 2-methoxyestradiol on apoptosis and HIF-1a and HIF-2a expression in lung cancer cells under normoxia and hypoxia. ONCOLOGY REPORTS. 2015-08-20 [PMID: 26548300] (WB, ICC/IF, Human)
• Zhang J, Wang C, Chen X et al. EglN2 associates with the NRF1-PGC1alpha complex and controls mitochondrial function in breast cancer. EMBO J 2015-12-02 [PMID: 26492917] (WB, Human)
• Karsikas S, Myllymaki M, Heikkila M et al. HIF-P4H-2 deficiency protects against skeletal muscle ischemia-reperfusion injury. J Mol Med (Berl) 2016-03-01 [PMID: 26452676]
• Saini Y, Proper SP, Dornbos P et al. Loss of Hif-2alpha Rescues the Hif-1alpha Deletion Phenotype of Neonatal Respiratory Distress In Mice. PLoS One 2015-01-01 [PMID: 26422241] (IF/IHC, Mouse)
• Bustamante-Marin XM, Cook MS, Gooding J et al. Left-Biased Spermatogenic Failure in 129/SvJDnd1Ter/+ Mice Correlates with Differences in Vascular Architecture, Oxygen Availability, and Metabolites 1 Biol Reprod. 2015-02-06 [PMID: 26224005] (WB, Mouse)
• Zeng Z, Huang WD, Gao Q et al. Arnebin-1 promotes angiogenesis by inducing eNOS, VEGF and HIF-1a expression through the PI3K-dependent pathway Int. J. Mol. Med. 2015-07-22 [PMID: 26202335] (WB, Human)
• Chen X, Liu J, He B et al. Vascular endothelial growth factor (VEGF) regulation by hypoxia inducible factor-1 alpha (HIF1A) starts and peaks during endometrial breakdown, not repair, in a mouse menstrual-like model Hum. Reprod. 2015-06-25 [PMID: 26113661] (IHC-P, Mouse)
  
  Details:
  HIF1 alpha antibody was used at 1:100 dilution for IHC-P analysis of 5um paraffin sections of uteri tissues from mouse menstrual-like model (details in Fan et al Faseb J 2008 22 3571- 3580).
• Liu J, You P, Chen G et al. Hyperactivated FRS2a-mediated signaling in prostate cancer cells promotes tumor angiogenesis and predicts poor clinical outcome of patients Oncogene. 2015-06-22 [PMID: 26096936] (WB, Human)
• Mirtschink P, Krishnan J, Grimm F et al. HIF-driven SF3B1 induces KHK-C to enforce fructolysis and heart disease. Nature 2015-06-25 [PMID: 26083752] (WB, Mouse)
• Guo C, Zhang Y, Wang T, Zhong M. Intranasal deferoxamine attenuates synapse loss via up?regulating the P38/HIF?1alpha pathway on the brain of APP/PS1 transgenic mice. Frontiers in Aging Neuroscience. 2015-06-02 [PMID: 26082716] (WB, IHC-P, Mouse)
• Zhao H, Wu Y, Chen Y, Liu H. Clinical significance of hypoxia-inducible factor 1 and VEGF-A in osteosarcoma Int. J. Clin. Oncol. 2015-06-16 [PMID: 26077139] (WB, IHC-P, Human)
• Xie L, Pi X, Townley-Tilson WH et al. PHD2/3-dependent hydroxylation tunes cardiac response to beta-adrenergic stress via phospholamban. J Clin Invest 2015-07-01 [PMID: 26075818] (WB)
• Yorulmaz H, Ozkok E, Erguven M et al. Effect of simvastatin on mitochondrial enzyme activities, ghrelin, hypoxia-inducible factor 1alpha in hepatic tissue during early phase of sepsis. Int J Clin Exp Med 2015-01-01 [PMID: 26064259] (IF/IHC, Rat)
• Zieseniss A, Hesse AR, Jatho A et al. Cardiomyocyte-Specific Transgenic Expression of Prolyl-4-Hydroxylase Domain 3 Impairs the Myocardial Response to Ischemia. Cell Physiol Biochem 2015-05-27 [PMID: 26044310]
• Hwang HJ, Lynn SG, Vengellur A et al. Hypoxia Inducible Factors Modulate Mitochondrial Oxygen Consumption and Transcriptional Regulation of Nuclear-Encoded Electron Transport Chain Genes. Biochemistry 2015-06-23 [PMID: 26030260]
• Valbuena GN, Rizzardini M, Cimini S et al. Metabolomic Analysis Reveals Increased Aerobic Glycolysis and Amino Acid Deficit in a Cellular Model of Amyotrophic Lateral Sclerosis. Mol. Neurobiol. 2015-05-12 [PMID: 25963727] (WB, Mouse, Human)
• Conley SJ, Baker TL, Burnett JP et al. CRLX101, an investigational camptothecin-containing nanoparticle-drug conjugate, targets cancer stem cells and impedes resistance to antiangiogenic therapy in mouse models of breast cancer Breast Cancer Res. Treat. 2015-04-01 [PMID: 25833208] (IF/IHC, WB, Human)
• Tan SC, Gomes RS, Yeoh KK et al. Preconditioning of cardiosphere-derived cells with hypoxia or prolyl-4-hydroxylase inhibitors increases stemness and decreases reliance on oxidative metabolism. Cell Transplant. 2015-03-06 [PMID: 25751158] (WB, Rat)
• Albers J, Danzer C, Rechsteiner M et al. A versatile modular vector system for rapid combinatorial mammalian genetics. J Clin Invest 2015-04-01 [PMID: 25751063] (WB)
• Lee M, Choi SY, Kang BH et al. Differential microscopic finding and glucose transporter 3 expression in terminal chorionic villi among birth weight-discordant twin placentas Histol. Histopathol. 2015-03-02 [PMID: 25728965] (IHC-P)
• Kato H, Izumi K, Uenoyama A et al. Hypoxia Induces an Undifferentiated Phenotype of Oral Keratinocytes in vitro Cells Tissues Organs (Print). 2015-02-18 [PMID: 25720390]
• Qiao N, Xu C, Zhu YX et al. Ets-1 as an early response gene against hypoxia-induced apoptosis in pancreatic beta-cells Cell Death Dis. 2015-02-19 [PMID: 25695603]
• Stantic M, Sakil HA, Zirath H et al. TAp73 suppresses tumor angiogenesis through repression of proangiogenic cytokines and HIF-1alpha activity.. Proc Natl Acad Sci U S A. 2015-01-06 [PMID: 25535357] (WB, Chemotaxis)
• Sato Yoshifumi, Inoue Masahiro, Yoshizawa Tatsuya, Yamagata Kazuya. Moderate hypoxia induces beta-cell dysfunction with HIF-1-independent gene expression changes. PLoS One. 2014-12-12 [PMID: 25503986] (WB)
• Takamiya R, Takahashi M, Uehara Y, Ariki S. The single N-glycan deletion mutant of soluble ErbB3 protein attenuates heregulinb1-induced tumor progression by blocking of the HIF-1 and Nrf2 pathway. Biochem Biophys Res Commun. 2014-10-24 [PMID: 25451255] (WB, Human)
  
  Details:
  HIF1 alpha antibody used for WB on experiments involving MCF7, T47D and BT474 cells
• Fine Jm, Renner Db, Forsberg Ac et al. Intranasal deferoxamine engages multiple pathways to decrease memory loss in the APP/PS1 model of amyloid accumulation. Neurosci. Lett. 2014-11-13 [PMID: 25445365] (WB, Mouse)
• Xie Yuan, Zhang Jin, Lin Ying et al. Defining the role of oxygen tension in human neural progenitor fate. Stem Cell Reports. 2014-11-11 [PMID: 25418722] (ICC/IF, Human)
• Lam Gc, Sefton Mv. Tuning Graft- and Host-Derived Vascularization in Modular Tissue Constructs: A Potential Role of HIF1 Activation Tissue Eng Part A et al. 2014-11-07 [PMID: 25379774] (IHC-P, Human)
  
  Details:
  HIF1 alpha antibody used for IHC-P on human modular tissues /sub-millimeter collagen modules containing adipose-derived mesenchymal stromal cells (adMSC) and enveloped by human umbilical vein endothelial cells (HUVEC) subcutaneously implanted in severe-combined immunodeficient mice with a beige-mutation (SCID-bg) mice - 24 hour fixation in 10% neutral buffered formalin, 4um paraffin sections, 1:2000 primary dilution,
• Clarke Rh, Moosa S, Anzivino M et al. Sustained radiosensitization of hypoxic glioma cells after oxygen pretreatment in an animal model of glioblastoma and in vitro models of tumor hypoxia. PLoS OnE. 2014-10-29 [PMID: 25350400] (WB, Human)
• Takahashi H, Inoue A, Kawabe Y et al. Oct-3/4 promotes tumor angiogenesis through VEGF production in glioblastoma Brain Tumor Pathol et al. 2014-10-28 [PMID: 25348671] (WB, ICC/IF, Human)
  
  Details:
  HIF1 alpha antibody used for WB and ICC-IF on U251/EGFP or U251/EGFP-Oct-3/4 cells (Fig 5). WB also performed on cells subjected to Oct-3/4 knockdown (Fig 6b) and U251/EGFP-Oct-3/4 cells were treated with AKT inhibitor (Fig 6c).
• Burgi S, Seuwen A, Keist R et al. In Vivo Imaging of Hypoxia-Inducible Factor Regulation in a Subcutaneous and Orthotopic GL261 Glioma Tumor Model Using a Reporter Gene Assay. Mol Imaging. 2014-09-01 [PMID: 25248521]
• Okon IS, Coughlan KA, Zhang C et al. Protein kinase LKB1 promotes RAB7-mediated neuropilin-1 degradation to inhibit angiogenesis. J Clin. Invest. 2014-09-02 [PMID: 25180605] (WB, IP, Human)
• Huang BW, Miyazawa M, Tsuji Y et al. Distinct Regulatory Mechanisms of the Human Ferritin Gene by Hypoxia and Hypoxia Mimetic Cobalt Chloride at the Transcriptional and Post-transcriptional Levels. Cell. Signal. 2014-08-26 [PMID: 25172425] (WB, Human)
• Hesse AR, Levent E, Zieseniss A et al. Lights on for HIF-1a: Genetically Enhanced Mouse Cardiomyocytes for Heart Tissue Imaging. Cell. Physiol. Biochem. 2014-07-30 [PMID: 25095893] (WB, Mouse)
  
  Details:
  HIF-1 alpha antibody used for WB on protein extracts of hypoxia exposed ODD-Luc-CMs/Cardiomyocytes derived from an embryonic stem cell (ESC) lines (ODD-Luc ESCs) that was generated from a Tg ROSA26 ODD-Luc/+ mouse (Figure 2D).
• Kaasinen E, Rahikkala E, Koivunen P et al. Clinical characterization, genetic mapping and whole-genome sequence analysis of a novel autosomal recessive intellectual disability syndrome. Eur J Med Genet 2014-07-28 [PMID: 25078763] (WB, Human)
  
  Details:
  HIF1a/HIF-1 alpha antibody used for WB on proteins extracts from human lymphoblasts cultured under normoxic and hypoxic conditions. Lymphoblasts were from six patients who developed an unexplained syndromic form of profound intellectual disability (Supplementary Fig. 2b and c).
• Ueda J, Ho JC, Lee KL et al. The Hypoxia-Inducible Epigenetic Regulators Jmjd1a and G9a Provide a Mechanistic Link between Angiogenesis and Tumor Growth. Mol. Cell. Biol. 2014-07-28 [PMID: 25071150] (WB, Mouse)
  
  Details:
  JMJD1A antibody used in WB on wild-type (WT) and Jmjd1a knockout (KO1 and KO2) embryonic stem cells (Fig 2B), 1% hypoxia exposed WT ES cells (Fig 4A), WT or Jmjd1a KO ES allograft tumors (8D) and Jmjd1a KO ES cells stably transfected with G9a shRNA (Fig 10A).
• Abu-Remaileh M, Aqeilan RI. Tumor suppressor WWOX regulates glucose metabolism via HIF1alpha modulation. Cell Death Differ 2014-11-01 [PMID: 25012504] (WB)
• Slominski A, Kim TK, Brozyna AA et al. The role of melanogenesis in regulation of melanoma behavior: Melanogenesis leads to stimulation of HIF-1a expression and HIF-dependent attendant pathways. Arch. Biochem. Biophys. 2014-07-02 [PMID: 24997364] (WB, Hamster, Human)
  
  Details:
  HIF-1 alpha antibody used for WB on whole cell lysates of Bomirski hamster AbC1 and human SKMEL-188 stable lined (amelanotic /non-pigmented and melanotic /pigmented lines) with Hif1a wild type/WT and knockout /KO mammary tumor epithelial cells (MTECs) derived from the polyoma virus middle T transgenic mouse (MMTV-PyMT) as positive control for HIF-1 alpha. Antibody used at 1:5000 dilution and the data is shown in Fig 1.
• Kono K, Tamashiro DA, Alarcon VB. Inhibition of RHO-ROCK signaling enhances ICM and suppresses TE characteristics through activation of hippo signaling in the mouse blastocyst. Dev. Biol. 2014-07-02 [PMID: 24997360] (WB, Human, Hamster)
  
  Details:
  HIF-2 alpha antibody used for WB on lysates of Bomirski hamster AbC1 and human SKMEL-188 cell lines (Fig. 1).
• Zheng Xingnan, Zhai Bo, Koivunen Peppi et al. Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase. Genes Dev. 2014-07-01 [PMID: 24990963] (WB, Mouse)
• Chen RL, Ogunshola OO, Yeoh KK et al. HIF prolyl hydroxylase inhibition prior to transient focal cerebral ischaemia is neuroprotective in mice J. Neurochem. 2014-06-26 [PMID: 24974727] (WB, IHC-P, Rat, Mouse)
  
  Details:
  HIF-1 alpha antibody used for IHC-P on FFPE tissue sections of C57/B6 mouse brains (in vivo transient focal cerebral ischaemia model) treated or not with 2-(1-chloro-4-hydroxyisoquinoline-3-carboxamido) acetic acid (IOX3), a selective small molecule inhibitor of hypoxia-inducible factor (HIF) prolyl hydroxylases (staining images - Figure 4). Antibody also used for WB on rat brain endothelial cell line RBE4 exposed to IOX3 and hypoxia (Figure 6)
• Peng YJ, Yuan G, Khan S et al. Regulation of Hypoxia Inducible Factor-alpha Isoforms and Redox State By Carotid Body Neural Activity in Rats. J. Physiol. (Lond.). 2014-06-27 [PMID: 24973414] (WB, IHC-Fr, Rat)
• Mangiavini L, Merceron C, Araldi E et al. Loss of VHL in mesenchymal progenitors of the limb bud alters multiple steps of endochondral bone development Dev. Biol. 2014-06-24 [PMID: 24972088] (WB, Mouse)
  
  Details:
  HIF-1 alpha antibody used for Western blot at 1:1000 dilution in protein lysate from chondrocytes of CNTRL and VHL mice ( protocol details provided in this article, WB image - Supplemental Fig. 1, Panel B)
• Wojtal K A, Cee A et al. Downregulation of duodenal SLC transporters and activation of proinflammatory signaling constitute the early response to high altitude in humans. Am J Physiol Gastrointest Liver Physiol 2014-01-10 [PMID: 24970780] (WB, Human)
• Ghim J, Moon J S et al. Endothelial deletion of phospholipase D2 reduces hypoxic response and pathological angiogenesis. Arterioscler Thromb Vasc Biol 2014-01-08 [PMID: 24947526] (WB, Mouse)
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  Details:
  Using the HRP conjugated version of NB100-479, catalog number NB100-479H.
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• Noguera R, Fredlund E, Piqueras M et al. HIF-1alpha and HIF-2alpha are differentially regulated in vivo in neuroblastoma: high HIF-1alpha correlates negatively to advanced clinical stage and tumor vascularization. Clin Cancer Res;15(23):7130-6. 2009-12-01 [PMID: 19903792] (IHC-P, Human)
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• Wasser, Y, Y The Role of Dlk1 in the Gene Regulatory Network Underlying Motor Neuron Diversification Thesis 2022-01-01 (IHC, WB, Mouse)
• Tawari EP. Investigation of chemoresistant mechanisms in triple negative breast. Thesis. 2016-03-01 (ICC/IF, WB, Human)
• Samuel T, Rapic S, Lindsay P, DaCosta R Investigating the Effects of Stereotactic Body Radiation Therapy on Pancreatic Tumor Hypoxia and Microvasculature in an Orthotopic Mouse Model using Intravital Fluorescence Microscopy Research Square 2023-09-25 (IHC-P, Mouse)
  
  Details:
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• Pham D, Nguyen T, Nguyen B et al. Adiponectin restores the obesity-induced impaired immunomodulatory function of mesenchymal stromal cells via glycolytic reprogramming Acta Pharmaceutica Sinica B 2023-10-01 (Immunocytochemistry/ Immunofluorescence, Mouse)
• Thiersch M, Lange C, Joly S et al. Retinal neuroprotection by hypoxic preconditioning is independent of hypoxia-inducible factor-1 alpha expression in photoreceptors. Eur J Neurosci 2009-06-01 [PMID: 19508692] (WB, Mouse)
• Lam G. Exploring the Role of Hypoxia-Related Parameters in the Vascularization of Modular Tissues. Thesis 2013-01-01 (IHC-P, Human)
• Kearon J. Selective damage to and protection of enteric neurons in vitro in models of ischemia and reperfusion. Thesis. 2014-01-01 (ICC/IF, IHC-P)
• Hansen SL, Trakooljul N, Liu H-C et al. Iron Transporters Are Differentially Regulated by Dietary Iron, Modifications Are Associated with Changes in Manganese Metabolism in Young Pigs. J Nutr;139(8):1474-1479. 2009-01-01 [PMID: 19535423]
• Akhtar R, Tahir H, Stewart E et al. Toll-Like Receptor Signalling Pathways Regulate Hypoxic Stress Induced Fibroblast Growth Factor but Not Vascular Endothelial Growth Factor-A in Human Microvascular Endothelial Cells International Journal of Translational Medicine 2021-05-27 (WB, Human)
• Seavey MM, Maciag PC, Al-Rawi N et al. An anti-vascular endothelial growth factor receptor 2/fetal liver kinase-1 Listeria monocytogenes anti-angiogenesis cancer vaccine for the treatment of primary and metastatic Her-2/neu+ breast tumors in a mouse model. J Immunol 182(9):5537-46. 2009-05-01 [PMID: 19380802] (ICC/IF, IF/IHC, Mouse)
• Saini Y, Harkema JR, LaPres JJ. HIF1alpha is essential for normal intrauterine differentiation of alveolar epithelium and surfactant production in the newborn lung of mice. J Biol Chem 283(48):33650-7. 2008-11-28 [PMID: 18801745] (WB, Mouse)
• Ramanathan M, Pinhal-Enfield G, Hao I et al. Synergistic up-regulation of vascular endothelial growth factor (VEGF) expression in macrophages by adenosine A2A receptor agonists and endotoxin involves transcriptional regulation via the hypoxia response element in the VEGF promoter. Mol Biol Cell 18(1):14-23. 2007-01-01 [PMID: 17065555] (WB, Mouse)
• Dai X, Ruan J, Guo Y et al. Enhanced Radiotherapy Efficacy and Induced Anti-tumor Immunity in HCC by Improving Hypoxia Microenvironment using Oxygen Microcapsules Chemical Engineering Journal 2021-04-01 (IF/IHC, ICC/IF)
• Deli L, Wittmann G, Kallo I et al. Type 1 cannabinoid receptor-containing axons innervate hypophysiotropic thyrotropin-releasing hormone-synthesizing neurons. Endocrinology 2009-01-01 [PMID: 18818298] (IF/IHC, Mouse)
• Kariuki TM, Van Dam GJ, Deelder AM et al. Previous or ongoing schistosome infections do not compromise the efficacy of the attenuated cercaria vaccine. Infect Immun 2006-07-01 [PMID: 16790771]
• Gamal-Eldeen A, Baghdadi H, Afifi N, et al. Gum arabic-encapsulated gold nanoparticles modulate hypoxamiRs expression in tongue squamous cell carcinoma Molecular & Cellular Toxicology 2021-01-26
• Zhang J, Liu A, Hou R et al. Salidroside protects cardiomyocyte against hypoxia-induced death: a HIF-1alpha-activated and VEGF-mediated pathway. Eur J Pharmacol 2009-04-01 [PMID: 19326475] (Rat)
• Minamishima YA, Moslehi J, Bardeesy N et al. Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failure. Blood;111(6):3236-44. 2008-03-15 [PMID: 18096761] (WB, Mouse)
• Liao D, Corle C, Seagroves TN et al. Hypoxia-inducible factor-1alpha is a key regulator of metastasis in a transgenic model of cancer initiation and progression. Cancer Res 67(2):563-72. 2007-01-15 [PMID: 17234764] (WB, Mouse)
• Bayele HK, Peyssonnaux C, Giatromanolaki A et al. HIF-1 regulates heritable variation and allele expression phenotypes of the macrophage immune response gene SLC11A1 from a Z-DNA forming microsatellite. Blood 110(8):3039-48. 2007-10-15 [PMID: 1760676] (WB, Mouse)
• Fu Y, Cen D, Zhang T et al. Implantable fibrous scaffold with hierarchical microstructure for the ‘on-site’ synergistic cancer therapy Chemical Engineering Journal 2020-12-01 (WB, Mouse)
• McLaren AT, Marsden PA, Mazer CD et al. Increased expression of HIF-1alpha, nNOS, and VEGF in the cerebral cortex of anemic rats. Am J Physiol Regul Integr Comp Physiol;292(1):R403-14. 2007-01-01 [PMID: 16973934] (WB, ICC/IF, IF/IHC, Rat)
• Menendez-Montes I, Escobar B, Gomez MJ Activation of amino acid metabolic program in response to impaired glycolysis in cardiac HIF1 deficient mice bioRxiv 2020-01-01 (IF/IHC, Mouse)
• Liu Y, Gu Y, Ng S et al. Circulating levels of hydroxylated bradykinin function as an indicator of tissue HIF-1alpha expression Science Bulletin 2020-01-01 (IHC-P, Human)
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• Tran MGB, Bibby BAS, Yang L et al. Independence of HIF1a and androgen signaling pathways in prostate cancer bioRxiv (IHC, Human)
• Reichert E Hypoxia-Inducible Factor 1-alpha Upregulated during Sepsis, Involved in Inflammation Thesis 2019-01-01 (WB, Rat)
• Ishikawa T, Nakashiro K, Klosek SK et al. Hypoxia enhances CXCR4 expression by activating HIF-1 in oral squamous cell carcinoma. Oncol Rep 2009-03-01 [PMID: 19212630] (WB, Human)
• Wirthner R, Wrann S, Balamurugan K et al. Impaired DNA double-str break repair contributes to chemoresistance in HIF-1{alpha}-deficient mouse embryonic fibroblasts. Carcinogenesis;29(12):2306-2316. 2008-01-01 [PMID: 18842680] (WB, Mouse)
• Ream MA, Chandra R, Peavey M et al. High oxygen prevents fetal lethality due to lack of catecholamines. Am J Physiol Regul Integr Comp Physiol 295(3):R942-53. 2008-09-01 [PMID: 18635452] (WB, Mouse)
• Kundu CN, Balusu R, Jaiswal AS et al. Adenomatous polyposis coli-mediated hypersensitivity of mouse embryonic fibroblast cell lines to methylmethane sulfonate treatment: implication of base excision repair pathways. Carcinogenesis 28(10):2089-95. 2007-10-01 [PMID: 1752206] (WB, Mouse)

Reviews for HIF-1 alpha Antibody (NB100-479) (29)

Average Rating: 4.4

(Based on 29 reviews)

We have 29 reviews tested in 6 species: Human, Mouse, Rat, Human and Human Breast Cancer Cell Lines, Human and Mouse, Other.

reviewed by: Verified Customer ChIP Human 05/01/2024

Summary

• Application: Chromatin Immunoprecipitation
• Sample Tested: HEK293
• Species: Human
• Lot: D156632

Comments

• Comments: ChIP, IP

reviewed by: Verified Customer WB Human 05/28/2021

Summary

• Application: Western Blot
• Sample Tested: A549 cells
• Species: Human
• Lot: AQ-2

reviewed by: Verified Customer WB Human 03/15/2021

Summary

• Application: Western Blot
• Sample Tested: Hep3B human hepatocellular carcinoma cell line
• Species: Human
• Lot: AN-1

reviewed by: Yongkang Yang WB Human and Mouse 10/10/2020

Summary

• Application: Western Blot
• Sample Tested: cell line, whole cell lysate
• Species: Human and Mouse
• Lot: AP-2

reviewed by: Verified Customer ChIP Human and Human Breast Cancer Cell Lines 05/17/2020

Summary

• Application: Chromatin Immunoprecipitation
• Sample Tested: Breast cancer cells
• Species: Human and Human Breast Cancer Cell Lines
• Lot: AN-1

reviewed by: Verified Customer WB Human 04/16/2019

Summary

• Application: Western Blot
• Sample Tested: 16-HBE
• Species: Human
• Lot: AL-6

reviewed by: Verified Customer WB Human 09/18/2018

Summary

• Application: Western Blot
• Sample Tested: Human Cancer Cells
• Species: Human
• Lot: AN-1

reviewed by: Verified Customer IHC-P Mouse 10/25/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: IHC on parrafin sections (mouse)
• Species: Mouse
• Lot: AK-4

reviewed by: Kristina Cook WB Human 02/23/2017

Summary

• Application: Western Blot
• Sample Tested: U251 glioma cell line; whole cell lysate
• Species: Human

Comments

• Comments: 4 hour hypoxia (0.5% oxygen) incubation

reviewed by: Verified Customer WB Mouse 09/21/2016

Summary

• Application: Western Blot
• Sample Tested: Mouse kidney
• Species: Mouse

reviewed by: Verified Customer WB Mouse 08/06/2016

Summary

• Application: Western Blot
• Species: Mouse

reviewed by: Zhongxiao Wang WB Mouse 10/05/2015

Summary

• Application: Western Blot
• Sample Tested: mouse retina
• Species: Mouse
• Lot: NB100-479

reviewed by: Verified Customer WB Human 08/23/2015

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell whole cell lysate
• Species: Human

reviewed by: Verified Customer WB Human 03/27/2015

Summary

• Application: Western Blot
• Sample Tested: Hela lysates treated with MG132
• Species: Human

reviewed by: Verified Customer WB Mouse 02/18/2015

Summary

• Application: Western Blot
• Sample Tested: Mouse heart
• Species: Mouse
• Lot: J1

reviewed by: Verified Customer ChIP Human 12/15/2014

Summary

• Application: Chromatin Immunoprecipitation
• Sample Tested: HEK293
• Species: Human
• Lot: AA-1

reviewed by: Verified Customer WB Mouse 12/12/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23232625
• Species: Mouse

reviewed by: Verified Customer WB Other 09/15/2014

Summary

• Application: Western Blot
• Sample Tested: SK-N-BE(2) whole cell lysate
• Species: Other
• Lot: AA-1

reviewed by: Xiuquan Luo WB Human 09/12/2014

Summary

• Application: Western Blot
• Sample Tested: HEK293 cell lysate
• Species: Human

reviewed by: Verified Customer WB Other 08/18/2014

Summary

• Application: Western Blot
• Sample Tested: PC12 whole cell lysate
• Species: Other
• Lot: AA-1

reviewed by: Ming Lu WB Human 08/15/2014

Summary

• Application: Western Blot
• Sample Tested: Human Pancreatic Cancer Cell line
• Species: Human
• Lot: AA

reviewed by: Molly Jenkins WB Human 07/18/2014

Summary

• Application: Western Blot
• Sample Tested: Nuclear fraction, Human cell line
• Species: Human

reviewed by: Jason Lee WB Human 10/29/2013

Summary

• Application: Western Blot
• Sample Tested: Human MCF7 breast epithelial cells
• Species: Human
• Lot: Lot X-1

reviewed by: Verified Customer IHC-P Mouse 06/27/2013

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: paraffin section of skin wound
• Species: Mouse

reviewed by: Sandhya Khurana WB Rat 02/05/2012

Summary

• Application: Western Blot
• Sample Tested: PC12 nuclear extract, Sample Amount: 50ug
• Species: Rat

reviewed by: Verified Customer WB Rat 01/03/2012

Summary

• Application: Western Blot
• Sample Tested: PC12 cell whole lysate, Sample Amount: 25ug
• Species: Rat
• Lot: P1

reviewed by: Verified Customer IHC-P Mouse 07/30/2011

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: normal mouse kidney
• Species: Mouse

reviewed by: Paramahamsa Maturu IHC-P Human 03/01/2011

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human Kidney tumor
• Species: Human
• Lot: I

reviewed by: JORCYK LAB WB Mouse 02/01/2011

Summary

• Application: Western Blot
• Sample Tested: 4T1 cells whole cell lysate
• Species: Mouse

FAQs for HIF-1 alpha Antibody (NB100-479). (Showing 1 - 10 of 17 FAQs).

1. Why is there a difference between the theoretical MW for HIF1A and the observed MW for HIF-1 alpha?  ..show answer..
   • HIF1A, like many other proteins, has post-translational modifications. Depending on the size, amount and nature of the post-translational modifications, it can cause subtle to very large changes in molecular weight.
2. Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.  ..show answer..
   • All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, we suggest the following four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products have been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449) (cited in at least 31 peer reviewed publications).
3. I would like to know, does a path exist for detection of HIF 1 in venous blood before and after revascularization of the leg?   ..show answer..
   • We are not entirely sure if HIF-1 alpha will be present in the leg after revascularization. It may be present, but you may want to search the literature to see if this has been looked at before. If not, then this would certainly be an experiment worth doing.
4. What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?  ..show answer..
   • The theoretical molecular weight of HIF 1-alpha is ~93kDa. However, you will likely see a band between 100-120kDa due to phosphorylation.
5. We got the Hif1a (NB100-105) antibody from you guys. I used the concentration that is mentioned on your website, but I am getting a band of a completely different size (~70kDa) and not the 120 kDa mentioned.  ..show answer..
   • HIF-1 alpha is a notoriously difficult protein to work with due to its rapid degradation. Therefore, the ~70kDa bands are most likely degradation products. It is very important to lyse the cells in hypoxic conditions. We strongly recommend lysing the cells directly into the Laemmli buffer and doing that quickly, so that the exposure to oxygen is minimized.Please go through our hypoxia related FAQs, you should find them very informative.Also, running a positive control may help confirm the band specificity in your samples. You may prepare them yourself or choose some from our catalog, for example: 1) HeLa Hypoxic / Normoxic Cell Lysate (NBP2-36452)2) HeLa Hypoxic (CoCl2) / Normoxic Cell Lysate (NBP2-36450)
6. I performed several Western Blots of HIF-1 alpha with different lysis buffers, whole lysates, and cytoplasm/nuclei extractions. I can’t seem to get a good western blot (poor signal, band much lower than expected, etc.). Can someone suggest some technical considerations/tricks I should consider using?  ..show answer..
   • A major issue that researchers working with HIF-1 alpha is degradation due to exposure to oxygen. In western blot, this results in a weaker band and/or the appearance of multiple low molecular weight bands (40-80 kDa). We recommend preparing the lysates after collection of cells/tissues as quickly as possible (on ice), preferably in a hypoxic chamber. We also recommend including a true hypoxia mimetic control (eg: cells treated with CoCl2, DMOG… etc.). The controls help distinguish your band of interest from potential degradation/dimer bands.For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.
7. I am doing HIF1 westerns in HIF-overexpressing mouse liver and adipose tissue using Novus antirabbit HIF1a antibody with overnight incubation. I am getting strong bands around 90kDa. I am aware that HIF theoretical molecular weight is 93kDa, but in westerns, the HIF band is usually around 120kDa according to my internet research. Can someone let me know if I’m getting the right HIF band or just some non-specific bands? Thanks.  ..show answer..
   • (1)    HIF-1 alpha’s theoretical molecular weight is 93kDa. The post translationally modified/ubiquitinated form of HIF-1 alpha protein (fails to undergo proteasomal degradation) shows up as a band in the 110-130 kDa range on a Western blot.(2)    The dimeric protein may appear at a position above 200 kDa on non-reducing gels.(3)    Importantly, HIFs are among the most rapidly degradable proteins; therefore, sample preparation is highly important when analyzing HIF1 alpha or HIF2 alpha. When degraded, HIF-1 alpha may show up between 40-80 kDa position on Western blot. Degradation may be avoided by preparing the samples as soon as possible after collection of cells/tissues in hypoxic chamber. Notably, the tissues/cells should be kept on ice during lysate preparation and the lysates should be analyzed as soon as possible.(4)    For troubleshooting suggestions/feedback on more than 25 similar frequently asked questions, I would recommend visiting Novus page: FAQs - Hypoxia and HIFs (5)    Last but not the least, Novus technical support team may be contacted via email
8. I have Hif1a nuclear protein extract at -80C. I am wondering if anyone knows how long it would be good for at that temperature since HIf1a is known to be degraded easily.Thank you!  ..show answer..
   • You could try a few things to further inhibit the degradation.1) Use the protease inhibitors (if you are not already using them).2) Lyse cells into a buffer that contains SDS or LDS (eg: Laemmli's buffer), since SDS and LDS denature and inhibit proteases. Lysis may even be performed with reducing agents in the buffer (eg. DTT), but this will make your lysates unsuitable for BCA assay.3) Lysing samples rapidly ensures that the samples are instantly homogenized (it also shears DNA released by the SDS).5) Flash-freezing samples in liquid nitrogen rather than freezing at -80*C reduces the window of time for protease activity.6) Freeze samples in individual aliquots, instead of thawing the same vial multiple times.
9. I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?  ..show answer..
   • CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.
10. I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?  ..show answer..
    • CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.
11. Is cross-reactivity with HIF-2 alpha tested/predicted?  ..show answer..
    • Although we don’t have cross-reactivity data with regards to HIF-2 alpha, we predict minimal cross-reactivity based on low sequence similarity observed from BLAST analysis between HIF-1 alpha and HIF-2 alpha.
12. Do you sell a positive and negative control for the HIF-1 alpha antibody (NB100-479)?  ..show answer..
    • For Western blot, the best positive control that we have is a CoCl2-treated Cos-7 cell lysate, catalog # NB800-PC26. The kit comes with a negative control as well. If you are doing ICC, then you could treat your cells with CoCl2 or expose them to hypoxic conditions.
13. What do think the blocking buffer should be?  ..show answer..
    • We recommend 5% non-fat dry milk in TBS.
14. We need to examine tissue-specific knockout of HIF-1a in mouse lungs. We have generated smooth muscle specific knockout of HIF-1a, and would like to demonstrate the knockdown in lung tissue sections by IHC or immunofluorescence. The problem is that all the antibodies for the mouse seem to have too much non-specific staining. Can you recommend an antibody that would be good for mouse lung tissue IHC or immunofluorescence?  ..show answer..
    • The antibody with Catalog # NB100-479 is one of our best sellers and has 5 star rating in customer reviews. Please view the product datasheet to see the publications in which researchers have cited this product, over 10 of which are involved in mouse models.
15. Is there a WB image for this antibody?  ..show answer..
    • We do have a WB image for the antibody base product. It looks like they ordered an HRP conjugated format, and that specific format page does not have the image. We do all of our testing on the base product.
16. Will this antibody detect HIF-1 alpha protein by WB on a breast cancer cell line in normal condition (without hypoxia)?  ..show answer..
    • If the breast cancer gene turned on the HIF1 alpha gene, there should be some expression of the protein, even without hypoxic induction. However, this expression may be very low, and depending on the length of time it took to lyse and/or fix the cells, may have degraded in the presence of oxygen. This may also be affected by the presence or lack of any HIF stabilizers in the lysate buffer. We talk about these stabilizers and other testing protocols in our white paper here. I would recommend trying to detect HIF expression in cancer cell lines that you run both positive and negative controls along side the cell lines to ensure that any bands detected are in fact HIF1 alpha.
17. Does HIF-1 alpha antibody NB100-479 cross react with HIF-2?  ..show answer..
    • None of the QC experiments conducted by the lab showed substantial background/band similar to HIF-2a. Additionally, a BLAST search comparing the immunogen of NB100-479 and the amino acid sequence of human HIF-1 alpha shows the homology between the two is low.
Show All 17 FAQs.

Bioinformatics

• Gene Symbol: HIF1A
• Entrez:
  • 3091(Human)
  • 15251(Mouse)
  • 29560(Rat)
• Uniprot:
  • Q61221(Mouse)