Histone H2A.Z Antibody (ST46-09)

Immunocytochemistry/Immunofluorescence: Histone H2A.Z Antibody (ST46-09) [NBP2-67499] - Staining of Histone H2A.Z in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Immunohistochemistry-Paraffin: Histone H2A.Z Antibody (ST46-09) [NBP2-67499] - Human kidney tissue with Rabbit anti-Histone H2A.Z antibody at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/Immunofluorescence: Histone H2A.Z Antibody (ST46-09) [NBP2-67499] - Staining Histone H2A.Z in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Histone H2A.Z Antibody (ST46-09) [NBP2-67499] - Analysis of paraffin-embedded human kidney tissue using anti-Histone H2A.Z antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Histone H2A.Z Antibody (ST46-09) [NBP2-67499] - Analysis of paraffin-embedded human lung cancer tissue using anti-Histone H2A.Z antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Histone H2A.Z Antibody (ST46-09) [NBP2-67499] - Analysis of paraffin-embedded human spleen tissue using anti-Histone H2A.Z antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Histone H2A.Z Antibody (ST46-09) [NBP2-67499] - Analysis of paraffin-embedded mouse colon tissue using anti-Histone H2A.Z antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Histone H2A.Z Antibody (ST46-09) [NBP2-67499] - Human liver tissue with Rabbit anti-Histone H2A.Z antibody at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: ST46-09
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Histone H2A.Z Antibody (ST46-09) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human Histone H2A.Z aa 1-50. (SwissProt: P0C0S5 Human; SwissProt: P0C0S6 Mouse; SwissProt: P0C0S7 Rat)
• Localization: Nucleus, Chromosome, Nucleosome core.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: H2AZ1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Histone H2A.Z Antibody (ST46-09)

• H2A histone family, member Z
• H2A.z
• H2A/z
• H2AZ histone
• H2AZMGC117173
• histone H2A.Z

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2AZ1