![Western Blot: Histone H4 [Monomethyl Arg3] Antibody (RM195) [NBP2-61556] - Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. using NBP2-61556, showed a band of Histone monomethylated at Arginine 3 in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Arg3-Antibody-RM195-Western-Blot-NBP2-61556-img0006.jpg "Western Blot: Histone H4 [Monomethyl Arg3] Antibody (RM195) [NBP2-61556] - Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. using NBP2-61556, showed a band of Histone monomethylated at Arginine 3 in HeLa cells.")
| Reactivity | Hu, VbSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, Func |
| Clone | RM195 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | A monomethyl -peptide corresponding to the Histone H4 [Monomethyl Arg3](RM195) |
| Modification | Monomethyl Arg3 |
| Specificity | This antibody reacts to Histone H4 monomethylated at Arginine 3. No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | H4C16 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 50% Glycerol/PBS, 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H4 Antibody (NBP2-61556)Find related products by research area.
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| Gene Symbol | H4C16 |
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Arg3] Antibody (RM195) [NBP2-61556] - Immunocytochemical staining of HeLa cells using NBP2-61556 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Arg3-Antibody-RM195-Immunocytochemistry-Immunofluorescence-NBP2-61556-img0005.jpg "Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Arg3] Antibody (RM195) [NBP2-61556] - Immunocytochemical staining of HeLa cells using NBP2-61556 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Multiplex Immunoassay: Histone H4 [Monomethyl Arg3] Antibody (RM195) [NBP2-61556] - NBP2-61556 specifically reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arg 3 (R3 ctrl), asymmetric dimethylated Arg 3 (R3me2a), symmetric dimethylated Arg 3 (R3me2s), or citrullinated Arg 3 (R3Cit) in Histone H4.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Arg3-Antibody-RM195-Multiplex-Immunoassay-NBP2-61556-img0007.jpg "Multiplex Immunoassay: Histone H4 [Monomethyl Arg3] Antibody (RM195) [NBP2-61556] - NBP2-61556 specifically reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arg 3 (R3 ctrl), asymmetric dimethylated Arg 3 (R3me2a), symmetric dimethylated Arg 3 (R3me2s), or citrullinated Arg 3 (R3Cit) in Histone H4.")
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