HLA DQ/DR/DP Antibody (rHLA-Pan/3475)

Western Blot: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808] - Western Blot Analysis of (1) Ramos cell and (2) human spleen tissue lysate using HLA DQ/DR/DP antibody (rHLA-Pan/3475).

Immunocytochemistry/Immunofluorescence: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808] - Immunofluorescence Analysis of Human Raji cells with HLA DQ/DR/DP Mouse Recombinant Monoclonal Antibody (rHLA DQ/DR/DP/3475) followed by goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red).

Immunohistochemistry-Paraffin: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808] - Formalin-fixed, paraffin-embedded human Tonsil stained with HLA DQ/DR/DP Mouse Recombinant Monoclonal Antibody (rHLA DQ/DR/DP/3475).

Flow Cytometry: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808] - Flow Cytometric Analysis of Human Raji cells using HLA DQ/DR/DP Mouse Recombinant Monoclonal Antibody (rHLA DQ/DR/DP/3475)followed by goat anti- Mouse IgG-CF488 (Blue); Isotype control (Red).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: rHLA-Pan/3475
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

HLA DQ/DR/DP Antibody (rHLA-Pan/3475) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08644)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cells
• Localization: Cell Surface
• Specificity: Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DQA1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for HLA DQ/DR/DP Antibody (rHLA-Pan/3475)

• CD
• CELIAC1DQ alpha 1 chain
• DC-1 alpha chain
• DQ-A1
• FLJ27088
• FLJ27328
• GSE
• HLA class II histocompatibility antigen, DQ(W3) alpha chain
• HLA-DCA
• HLA-DQA
• leucocyte antigen DQA1
• leukocyte antigen alpha chain
• major histocompatibility complex, class II, DQ alpha 1
• MGC149527
• MHC class II antigen
• MHC class II DQA1
• MHC class II HLA-D alpha glycoprotein
• MHC class II HLA-DQ-alpha-1
• MHC class II surface glycoprotein
• MHC HLA-DQ alpha

Background

The major histocompatibility complex (MHC) is a large genomic region or gene family found in most vertebrates containing many genes with important immune system roles. In humans, the MHC spans almost 4 megabases of chromosome 6 and includes more than 200 known genes, of which about half have known immmunological functions. The best known genes in the MHC region are the subset that encodes cell-surface antigen-presenting proteins. In humans, these genes are referred to as human leukocyte antigen (HLA) genes. The most intensely studied HLA genes are: HLA A, HLA B, HLA C, HLA DPA, HLA DPB1, HLA DQA1, HLA DQB1, HLA DRA, and HLA DRB1. In humans, the HLA is divided into three regions: Class I, II, and III. The A, B, and C genes belong to HLA class I while the six D genes belong to class II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DQA1
• Uniprot:
  • P01903()
  • P01908(Human)
  • P01909(Human)
  • P01920(Human)
  • P04440(Human)