HO-1/HMOX1/HSP32 Antibody (1D6) - BSA Free

Western Blot: HO-1/HMOX1/HSP32 Antibody (1D6) [NBP2-59492] - Lane 1: HeLa cell lysates, Lane 2: A549 cell lysates, Lane 3: 293T cell lysates, Lane 4: HepG2 cell lysates, Lane 5: TF-1 cell lysates

Immunocytochemistry/Immunofluorescence: HO-1/HMOX1/HSP32 Antibody (1D6) [NBP2-59492] - Analysis of HMOX in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HMOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of HMOX in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HMOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)

Flow Cytometry: HO-1/HMOX1/HSP32 Antibody (1D6) [NBP2-59492] - Analysis of HMOX in HeLa cells. The cell was stained with NBP2-59492 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Product Discontinued

HO-1/HMOX1/HSP32 Antibody (1D6) - BSA Free Summary

• Immunogen: Recombinant human HO-1/HMOX1/HSP32 (1-266aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HMOX1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:10-1:1085
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HO-1/HMOX1/HSP32 Antibody (1D6) - BSA Free

• bK286B10
• EC 1.14.99.3
• heat shock protein, 32-kD
• heme oxygenase (decycling) 1
• HMOX1
• HO
• HO1
• HO-1
• HO-1heme oxygenase 1
• HSP32

Background

Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMOX1