HP1 alpha Antibody - BSA Free

Western Blot: HP1 alpha Antibody [NB110-40623] - Whole cell lysate (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB110-40623 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).

Immunohistochemistry-Paraffin: HP1 alpha Antibody [NB110-40623] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CBX5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HP1 alpha Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 150 and the C-terminus (residue 191) of human Chromobox Homolog 5 using the numbering given in entry NP_036249.1 (GeneID 23468).
• Marker: Heterochromatin Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CBX5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Immunohistochemistry 1:200-1:10000
  • Immunohistochemistry-Paraffin 1:200-1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:1000-1:10000
• Application Notes: Chip, IP reactivity reported in (PMID: 23045645). Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Control:

  HP1 alpha Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HP1 alpha Antibody - BSA Free

• Antigen p25
• chromobox homolog 5 (Drosophila HP1 alpha)
• chromobox homolog 5 (HP1 alpha homolog, Drosophila)
• chromobox homolog 5
• chromobox protein homolog 5
• Heterochromatin protein 1 homolog alpha
• heterochromatin protein 1-alpha
• HP1 alpha homolog
• HP1 alpha
• HP1
• HP1A
• HP1-ALPHA
• HP1Hs alpha
• HP1Hs-alpha

Background

CBX5 (chromobox protein homolog 5), also known as HP1 (heterochromatin protein 1 homolog alpha), is one of 3 isoforms of HP1 that binds heterochromatin and functions as a structural adaptor to stabilize and organize macromolecular complexes with nucleosomes for higher chromatin structure. All of the HP1 isoforms possess a chromodomain and chromoshadow domain and associate with methylated histones that mediate transcriptional repression. Alternate names for CBX5 include HP1 alpha, antigen p25, HP1Hs-alpha, and HP1A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HP1 alpha Antibody (NB110-40623)(6)

Publications using NB110-40623

• Bawa-Khalfe T. Isolation of In Vivo SUMOylated Chromatin-Bound Proteins. Methods Mol. Biol. 2016-09-16 [PMID: 27631808] (IP)
• Zhu H, Ren S, Bitler BG et al. SPOP E3 Ubiquitin Ligase Adaptor Promotes Cellular Senescence by Degrading the SENP7 deSUMOylase. Cell Rep. 2015-11-10 [PMID: 26527005] (Chemotaxis, Human)
• Bawa-Khalfe T, Lu LS, Zuo Y et al. Differential expression of SUMO-specific protease 7 variants regulates epithelial-mesenchymal transition Proc Natl Acad Sci U S A 2012-10-08 [PMID: 23045645] (Chemotaxis, IP, Human)
• Wang C, Zhang W, Chen X et al. The spreading of facultative H3K9me3-heterochromatin drives congenital disease bioRxiv 2023-08-14 (IP, Mouse)
• Maram Quttina, Kacie D. Waiters, Ashfia Fatima Khan, Samaneh Karami, Anthony S. Peidl, Mariam Funmi Babajide, Justus Pennington, Fatima A. Merchant, Tasneem Bawa-Khalfe, Jian-Ying Wang, Xiao-Di Tan Exosc9 Initiates SUMO-Dependent lncRNA TERRA Degradation to Impact Telomeric Integrity in Endocrine Therapy Insensitive Hormone Receptor-Positive Breast Cancer Cells 2023-10-20 [PMID: 37887339]
• Roworth AP, Carr SM, Liu G et al. Arginine methylation expands the regulatory mechanisms and extends the genomic landscape under E2F control Sci Adv 2019-06-01 [PMID: 31249870] (Chemotaxis, Human)

FAQs for HP1 alpha Antibody (NB110-40623). (Showing 1 - 1 of 1 FAQs).

1. I want to use the hp1 alpha antibody in zebrafish, and I think NB110-40623 might work. The online information of this product mentions that it recognize the region between residue 150 and the C-terminus (residue 191) of human Chromobox Homolog 5. Here is my question, do you use this 42 amino acid peptide as immunogen? If not, can you tell me the exact sequence of immunogen, or its identidy to zebrafish hp1 alpha whose access no. is AAI64295.1.
   • The immunogen for NB110-40623 was a synthetic peptide corresponding to the region 150-191, but the exact sequence can not be provided due to proprietary reasons. I did compare the homology between the human protein and the sequence you gave me, and it's greater than 95%.

Bioinformatics

• Gene Symbol: CBX5
• Entrez:
  • 23468(Human)
• Uniprot:
  • P45973(Human)