HRV3c Recognition Site Antibody

Western Blot: HRV3c Recognition Site Antibody [NBP2-29904] - Analysis of HRV3c performed on control protein containing GST & the HRV3c cleavage site. Antigen-antibody complexes: incubated 100ug HeLa lysate containing 2ug control protein with 4ug of an HRV3c pAb overnight on a rocking platform at 4C. Immune complexes were captured on 50ul of Protein A/G plus Agarose, washed extensively, & eluted with 5X Lane Marker Reducing Sample Buffer. Lysate & the immune complexes were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to PVDF membrane, & blocked with 5% BSA/TBS-0.1%Tween for 1 hr. Membrane was probed with GST monoclonal antibody at 1:1500 overnight rotating at 4C, washed in TBST, & probed with Clean-blot IP detection reagent at 1:2500 for at least 1 hr. Chemiluminescent detection performed using SuperSignal West Dura. Note: the IP fraction resulted in proteolytic activity of the GST-control protein.

Western Blot: HRV3c Recognition Site Antibody [NBP2-29904] - Analysis of 1ug of a control protein, either uncleaved (lane 1) or cleaved (lane 2) using the Human Rhinovirus 3C Protease (HRV 3C) per well.

• Reactivity: Hu, Ca, Pm, Pm-Cm, RM
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

HRV3c Recognition Site Antibody Summary

• Immunogen: Synthetic peptide recognizing the cleavage site of HRV3c protease
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 4 ug
  • Western Blot 1:1000 - 1:2000
• Application Notes: WB: Detect HRV3C cleavage site (Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro). IP: Used in HRV3C cleavage site-containing protein from a whole Hela cell lysate.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA and 30% glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HRV3c Recognition Site Antibody

• HRV3c
• Human Rhinovirus 3C
• Rhinovirus 3C

Background

Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. It cleaves between Gln and Gly (independent of Pro). (HRV) 3C Protease is used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is typically dual tagged for easy removal from the sample after cleavage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.