Human CXCL10/IP-10 Quantikine QuicKit ELISA

IP-10 is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Plasma recovery is 95% compared to 49% for the top competitor. In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices.

IP-10 is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 99%-124% compared to 112%-183% for the top competitor.

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human CXCL10/IP-10 Quantikine QuicKit ELISA Summary

• Background: The Quantikine^® QuicKit™ Human CXCL10/IP-10 Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human IP-10 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IP-10 and antibodies raised against the recombinant protein. Results obtained using natural human IP-10 showed linear curves that were parall...el to the standard curves obtained using the QuicKit™ standards. These results indicate that this kit can be used to determine relative mass values for natural human IP-10.
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• Specificity: Natural and recombinant human IP-10.
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CXCL10/IP-10 Quantikine QuicKit ELISA

• C7
• chemokine (C-X-C motif) ligand 10
• CRG2
• CRG-2
• CXCL10
• gIP-10
• IFI10
• INP10
• IP-10
• mob-1
• SCYB10

Background

IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.