Human IFN-beta Quantikine ELISA Kit

IFN-beta is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. R&D Systems recovery is 98% compared to 55% for the top competitor.

Samples containing high concentrations of IFN-beta in various matrices are diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. R&D Systems linearity is between 103%-112% compared to 119%-170% for the top competitor.

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IFN-beta Quantikine ELISA Kit Summary

• Background: The Quantikine® Human IFN-beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IFN-beta in cell culture supernates. It contains CHO cell-expressed recombinant human IFN-beta and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IFN-beta showed linear curves that were parallel to the standard curves obtained using t...he Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-beta .
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• Specificity: Natural and recombinant human IFN-beta .
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IFN-beta Quantikine ELISA Kit

• Fibroblast interferon
• IFB
• IFBIFNB
• IFF
• IFNB
• IFNB1
• IFNbeta
• IFN-beta
• interferon beta
• interferon, beta 1, fibroblast
• MGC96956

Background

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IFN-beta (DIFNB0)(28)

Publications using DIFNB0

• Zhang, J;Zhang, L;Liu, D;Shi, H;Zhang, X;Chen, J;Yang, X;Zeng, M;Zhang, J;Feng, T;Zhu, X;Jing, Z;Ji, Z;Shi, D;Feng, L; Helicase protein DDX11 as a novel antiviral factor promoting RIG-I-MAVS-mediated signaling pathway mBio 2024-10-29 [PMID: 39470258] (Human)
• Choi, JH;Luo, J;Hesketh, GG;Guo, S;Pistofidis, A;Ladak, RJ;An, Y;Naeli, P;Alain, T;Schmeing, TM;Gingras, AC;Duchaine, T;Zhang, X;Sonenberg, N;Jafarnejad, SM; Repression of mRNA translation initiation by GIGYF1 via disrupting the eIF3-eIF4G1 interaction Science advances 2024-07-19 [PMID: 39018414] (Human)
• Callon, D;Glenet, M;Lebreil, AL;Heng, L;Bouland, N;Fichel, C;Fornes, P;Andreoletti, L;Berri, F; Major Group-B Enterovirus populations deleted in the noncoding 5' region of genomic RNA modulate activation of the type I interferon pathway in cardiomyocytes and induce myocarditis PLoS pathogens 2024-05-02 [PMID: 38696536] (Mouse)
• Liu, X;Tang, J;Wang, Z;Zhu, C;Deng, H;Sun, X;Yu, G;Rong, F;Chen, X;Liao, Q;Jia, S;Liu, W;Zha, H;Fan, S;Cai, X;Gui, JF;Xiao, W; Oxygen enhances antiviral innate immunity through maintenance of EGLN1-catalyzed proline hydroxylation of IRF3 Nature communications 2024-04-26 [PMID: 38670937] (Human)
• Araya, R;Men, S;Uekusa, Y;Yu, Z;Kikuchi, H;Daitoku, K;Minakawa, M;Kawaguchi, S;Furukawa, KI;Oshima, Y;Imaizumi, T;Seya, K; The inhibitory effect of DIF-3 on polyinosinic-polycytidylic acid-induced innate immunity activation in human cerebral microvascular endothelial cells Journal of pharmacological sciences 2024-03-01 [PMID: 38395516] (Human)
• Reuschl, AK;Thorne, LG;Whelan, MVX;Ragazzini, R;Furnon, W;Cowton, VM;De Lorenzo, G;Mesner, D;Turner, JLE;Dowgier, G;Bogoda, N;Bonfanti, P;Palmarini, M;Patel, AH;Jolly, C;Towers, GJ; Evolution of enhanced innate immune suppression by SARS-CoV-2 Omicron subvariants Nature microbiology 2024-01-16 [PMID: 38228858] (Human)
• Murayama, T;Nakayama, J;Jiang, X;Miyata, K;Morris, AD;Cai, KQ;Prasad, RM;Ma, X;Efimov, A;Belani, N;Gerstein, ER;Tan, Y;Zhou, Y;Kim, W;Maruyama, R;Campbell, KS;Chen, L;Yang, Y;Balachandran, S;Canadas, I; Targeting DHX9 triggers tumor-intrinsic interferon response and replication stress in Small Cell Lung Cancer Cancer discovery 2024-01-08 [PMID: 38189443] (Human)
• A Hooftman, CG Peace, DG Ryan, EA Day, M Yang, AF McGettrick, M Yin, EN Montano, L Huo, JE Toller-Kaw, V Zecchini, TAJ Ryan, A Bolado-Car, AM Casey, HA Prag, ASH Costa, G De Los San, M Ishimori, DJ Wallace, S Venuturupa, E Nikitopoul, N Frizzell, C Johansson, A Von Kriegs, MP Murphy, C Jefferies, C Frezza, LAJ O'Neill Macrophage fumarate hydratase restrains mtRNA-mediated interferon production Nature, 2023-03-08;615(7952):490-498. 2023-03-08 [PMID: 36890227] (Human)
• JY Li, Y Zhao, S Gong, MM Wang, X Liu, QM He, YQ Li, SY Huang, H Qiao, XR Tan, ML Ye, XH Zhu, SW He, Q Li, YL Liang, KL Chen, SW Huang, QJ Li, J Ma, N Liu TRIM21 inhibits irradiation-induced mitochondrial DNA release and impairs antitumour immunity in nasopharyngeal carcinoma tumour models Nature Communications, 2023-02-16;14(1):865. 2023-02-16 [PMID: 36797289] (Human)
• J Nassour, LG Aguiar, A Correia, TT Schmidt, L Mainz, S Przetocka, C Haggblom, N Tadepalle, A Williams, MN Shokhirev, SC Akincilar, V Tergaonkar, GS Shadel, J Karlseder Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis Nature, 2023-02-08;614(7949):767-773. 2023-02-08 [PMID: 36755096] (Human)
• TK Kim, S Jeon, S Park, SK Sonn, S Seo, J Suh, J Jin, HY Kweon, S Kim, SH Moon, O Kweon, BH Koo, N Kim, HO Lee, YM Kim, YJ Kim, SH Park, GT Oh 2'-5' oligoadenylate synthetase?like 1 (OASL1) protects against atherosclerosis by maintaining endothelial nitric oxide synthase mRNA stability Nature Communications, 2022-11-04;13(1):6647. 2022-11-04 [PMID: 36333342] (Human, Mouse)
• CH Song, YH Kim, M Naskar, BW Hayes, MA Abraham, JH Noh, G Suk, MJ Kim, KS Cho, M Shin, EJ Lee, SN Abraham, HW Choi Lactobacillus crispatus Limits Bladder Uropathogenic E. coli Infection by Triggering a Host Type I Interferon Response Proceedings of the National Academy of Sciences of the United States of America, 2022-08-08;119(33):e2117904119. 2022-08-08 [PMID: 35939684] (Human)
• C Song, D Liu, S Liu, D Li, I Horecny, X Zhang, P Li, L Chen, M Miller, R Chowdhury, M Issa, R Shen, Y Yan, F Zhang, L Zhang, L Zhang, C Bai, J Feng, L Zhuang, R Zhang, J Li, H Wilkinson, J Liu, W Tao SHR1032, a novel STING agonist, stimulates anti-tumor immunity and directly induces AML apoptosis Scientific Reports, 2022-05-20;12(1):8579. 2022-05-20 [PMID: 35595822] (Cynomolgus Monkey, Human)
• KC Huang, D Chanda, S McGrath, V Dixit, C Zhang, J Wu, K Tendyke, H Yao, R Hukkanen, N Taylor, D Verbel, DS Kim, A Endo, TA Noland, Y Chen, M Matijevic, J Wang, J Hutz, N Sarwar, FG Fang, X Bao Pharmacological Activation of STING in Bladder Induces Potent Anti-tumor Immunity in Non-Muscle Invasive Murine Bladder Cancer Molecular Cancer Therapeutics, 2022-06-01;0(0):. 2022-06-01 [PMID: 35313332] (Mouse)
• Z Gao, X Zhang, L Zhang, S Wu, J Ma, F Wang, Y Zhou, X Dai, E Bullitt, Y Du, JT Guo, J Chang A yellow fever virus NS4B inhibitor not only suppresses viral replication, but also enhances the virus activation of RIG-I-like receptor-mediated innate immune response PloS Pathogens, 2022-01-21;18(1):e1010271. 2022-01-21 [PMID: 35061864] (Human)
• R Ishida, J Cole, J Lopez-Oroz, N Fayad, A Felix-Lope, M Elaish, SY Luo, O Julien, A Kumar, TC Hobman Mayaro Virus Non-Structural Protein 2 Circumvents the Induction of Interferon in Part by Depleting Host Transcription Initiation Factor IIE Subunit 2 Cells, 2021-12-12;10(12):. 2021-12-12 [PMID: 34944018] (Human)
• AH Pham, J Mitchell, S Botto, KM Pryke, VR DeFilippis, MH Hancock Human cytomegalovirus blocks canonical TGFbeta signaling during lytic infection to limit induction of type I interferons PloS Pathogens, 2021-08-19;17(8):e1009380. 2021-08-19 [PMID: 34411201] (Human)
• U Rand, SY Kupke, H Shkarlet, MD Hein, T Hirsch, P Marichal-G, L Cicin-Sain, U Reichl, D Bruder Antiviral Activity of Influenza A Virus Defective Interfering Particles against SARS-CoV-2 Replication In Vitro through Stimulation of Innate Immunity Cells, 2021-07-11;10(7):. 2021-07-11 [PMID: 34359926] (Human)
• Y Li, C Bax, J Patel, T Vazquez, A Ravishanka, MM Bashir, M Grinnell, D Diaz, VP Werth Plasma-derived DNA containing-extracellular vesicles induce STING-mediated proinflammatory responses in dermatomyositis Theranostics, 2021-05-21;11(15):7144-7158. 2021-05-21 [PMID: 34158841] (Human)
• G Miglietta, M Russo, RC Duardo, G Capranico G-quadruplex binders as cytostatic modulators of innate immune genes in cancer cells Nucleic Acids Research, 2021-07-09;0(0):. 2021-07-09 [PMID: 34139015] (Human)
• S Al-Beltagi, CA Preda, LV Goulding, J James, J Pu, P Skinner, Z Jiang, BL Wang, J Yang, AC Banyard, KH Mellits, P Gershkovic, CJ Hayes, J Nguyen-Van, IH Brown, J Liu, KC Chang Thapsigargin Is a Broad-Spectrum Inhibitor of Major Human Respiratory Viruses: Coronavirus, Respiratory Syncytial Virus and Influenza A Virus Viruses, 2021-02-03;13(2):. 2021-02-03 [PMID: 33546185] (Human)
• K Yu, H Tian, H Deng PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasion Sci Adv, 2020-11-20;6(47):. 2020-11-20 [PMID: 33219031] (Human)
• HJ Hurley, H Dewald, ZS Rothkopf, S Singh, F Jenkins, P Deb, S De, BJ Barnes, P Fitzgerald Frontline Science: AMPK regulates metabolic reprogramming necessary for interferon production in human plasmacytoid dendritic cells J. Leukoc. Biol., 2020-07-08;0(0):. 2020-07-08 [PMID: 32640499] (Human)
• JH Seo, CS Hwang, JY Yoo MTFMT deficiency correlates with reduced mitochondrial integrity and enhanced host susceptibility to intracellular infection Sci Rep, 2020-07-07;10(1):11183. 2020-07-07 [PMID: 32636430] (Human)
• SF Watson, N Bellora, S Macias ILF3 contributes to the establishment of the antiviral type I interferon program Nucleic Acids Res., 2020-01-10;0(0):. 2020-01-10 [PMID: 31701124] (Human)
• Y Wang, S Yuan, X Jia, Y Ge, T Ling, M Nie, X Lan, S Chen, A Xu Mitochondria-localised ZNFX1 functions as a dsRNA sensor to initiate antiviral responses through MAVS Nat. Cell Biol., 2019-11-04;21(11):1346-1356. 2019-11-04 [PMID: 31685995] (Mouse)
• Q Zhao, Y Wei, SJ Pandol, L Li, A Habtezion STING Signaling Promotes Inflammation in Experimental Acute Pancreatitis Gastroenterology, 2018-02-06;0(0):. 2018-02-06 [PMID: 29425920] (Human)
• H Mori, M Murakami, T Tsuda, K Kameda, R Utsunomiya, K Masuda, K Shiraishi, X Dai, M Tohyama, H Nakaoka, K Sayama Reduced-HMGB1 suppresses poly(I:C)-induced inflammation in keratinocytes J. Dermatol. Sci., 2018;0(0):. 2018 [PMID: 29395576] (ELISA Capture, Human)