Human IL-8/CXCL8 QuantiGlo ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-8/CXCL8 QuantiGlo ELISA Kit Summary

• Background: The QuantiGlo Human IL-8 Chemiluminescent Immunoassay is a 5 hour solid phase ELISA designed to measure human IL-8 levels in cell culture supernates, serum, and plasma. It contains the 72 amino acid variant of human IL-8 derived from E. coli as well as antibodies raised against this recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-8. ...Results obtained using natural human IL-8 showed linear curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-8.
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• Specificity: Natural and recombinant human IL-8
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CXCL8

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-8/CXCL8 QuantiGlo ELISA Kit

• 3-10C
• AMCF-I
• C-X-C motif chemokine 8
• CXCL8
• CXCL8SCYB8
• Emoctakin
• GCP1
• GCP-1TSG-1
• IL8
• IL-8
• interleukin 8
• K60
• LAI
• LECT
• LUCT
• LYNAP
• MDNCF
• MDNCFb-ENAP
• member 8
• MONAP
• MONAPGCP1
• NAF
• NAP1
• NAP-1NAP1
• NCF
• Neutrophil-activating protein 1
• Protein 3-10C
• T cell chemotactic factor
• T-cell chemotactic factor
• TCF
• TSG1

Background

Interleukin-8 (IL-8), also known as IL-8, GCP-1, and NAP-1, is a heparin-binding 8-9 kDa member of the alpha, or CXC family of chemokines. There are at least 15 human CXC family members that all adopt a three beta -sheet/one alpha -helix structure. Most CXC chemokines show an N-terminal Glu-Leu-Arg (ELR) tripeptide motif. IL-8 circulates as a monomer, homodimer, and heterodimer with CXCL4/PF4. The monomer is considered the most bio-active, while the heterodimer can potentiate PF4 activity. IL-8 oligomerization is modulated by its interactions with matrix and cell surface glycosaminoglycans (GAGs). Mature human IL-8 shares 65-69% amino acid (aa) identity with canine, feline, and porcine IL-8. There is no IL-8 gene counterpart in rodent. 

Multiple isoforms of IL-8 are generated through both alternative splicing and differential proteolytic cleavage. In humans, alternative splicing generates an iso-form with an eleven aa substitution at the C-terminus. Proteolytic processing results in N-terminal truncation of IL-8 and is likely a cell-specific event. For example, fibroblasts and endothelial cells generate the 1-77 form by cleaving IL-8 following Glu21, while monocytes and lymphocytes generate the 6-77 form by cleaving following Leu25. These truncated forms generally show increased bioactivity, particularly through the CXCR1 receptor. IL-8 can also undergo citrullination on Arg27 of the precursor, a modification that increases its half-life and ability to induce leukocytosis. A wide variety of cells secrete IL-8 including monocytes and neutrophils, fibroblasts and keratinocytes, mast cells, visceral smooth muscle cells, dendritic cells, type II great alveolar cells, and endothelial cells. 

IL-8 bioactivity is mediated through two G-protein-coupled receptors, termed CXCR1/IL-8 RA and CXCR2/IL-8 RB. CXCR1 is 45-50 kDa in size and is used almost exclusively by IL-8. CXCR2 is 35-40 kDa in size and is used by nearly all CXC chemokines. Both CXCR1 and CXCR2 constitutively associate into functional homodimers. They can also heterodimerize, but these complexes dissociate following IL-8 binding. CXCR2 responds to low concentrations of IL-8 and is principally associated with chemotaxis and MMP-9 release. CXCR1, in contrast, responds to high concentrations of IL-8 and is associated with respiratory burst and phospholipase D2 activation. Thus, CXCR2 ligation induces leukocyte adhesion to activated vascular endothelium and migration to sites of inflammation, while CXCR1 ligation primes neutrophil antimicrobial activity. IL-8 can also form a complex with Serpin A1/alpha-1 Antitrypsin, and this prevents IL-8 interaction with CXCR1. 

In addition to its pro-inflammatory effects, IL-8 is involved in angiogenesis and the pathogenesis of atherosclerosis and cancer. It induces VEGF expression in vascular endothelial cells and functions as an autocrine factor for EC growth and angiogenesis. It is upregulated in atherosclerotic lesions and is elevated in the serum and cerebrospinal fluid following myocardial infarction. In cancer, IL-8 promotes epithelial-mesenchymal transition as well as tumor cell invasiveness and metastasis.

Publications for CXCL8/IL-8 (Q8000B)(14)

Publications using Q8000B

• KT Tang, CC Lin, YH Chen, TL Liao, DY Chen, SS Yang, CC Chen Direct-Acting Antiviral Drugs Reduce Fibromyalgia Symptoms in Patients with Chronic Hepatitis C Journal of Clinical Medicine, 2022-09-10;11(18):. 2022-09-10 [PMID: 36142974] (Human)
• X Ma, W Yan, N He Lidocaine attenuates hypoxia/reoxygenation?induced inflammation, apoptosis and ferroptosis in lung epithelial cells by regulating the p38 MAPK pathway Molecular Medicine Reports, 2022-03-04;25(5):. 2022-03-04 [PMID: 35244190] (Human)
• S Karampoor, K Hesamizade, Z Shams, A Ghafari No, M Farahmand, H Zahednasab, R Mirzaei, F Zamani, M Hajibaba, B Bouzari, A Laali, A Tabibzadeh, M Hadi Karba, H Keyvani The role of lovastatin in the attenuation of COVID-19 International immunopharmacology, 2021-09-24;101(0):108192. 2021-09-24 [PMID: 34607230] (Human)
• O Vaší?ek, J Hájek, L Bláhová, P Hrouzek, P Babica, L Kubala, L Šindlerová Cyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model Harmful Algae, 2020-06-12;96(0):101849. 2020-06-12 [PMID: 32560836] (Human)
• M Sun, Y Huang, F Li, H Li, B Zhang, L Jin MicroRNA-874 inhibits TNF-?-induced remodeling in human fetal airway smooth muscle cells by targeting STAT3 Respir Physiol Neurobiol, 2018-02-12;0(0):. 2018-02-12 [PMID: 29448029] (Human)
• A Abrahamsso, A Capodanno, A Rzepecka, C Dabrosin Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women Oncotarget, 2017-09-15;8(54):92134-92142. 2017-09-15 [PMID: 29190903] (Human)
• Rovillain E, Mansfield L, Caetano C, Alvarez-Fernandez M, Caballero OL, Medema RH, Hummerich H, Jat PS Activation of nuclear factor-kappa B signalling promotes cellular senescence. Oncogene, 2011-01-17;30(20):2356-66. 2011-01-17 [PMID: 21242976] (Human)
• Hatzistilianou M, Rekliti A, Athanassiadou F, Catriu D Procalcitonin as an early marker of bacterial infection in neutropenic febrile children with acute lymphoblastic leukemia. Inflamm. Res., 2009-10-06;59(5):339-47. 2009-10-06 [PMID: 19806318] (Human)
• Gessner C, Dihazi H, Brettschneider S, Hammerschmidt S, Kuhn H, Eschrich K, Keller T, Engelmann L, Sack U, Wirtz H Presence of cytokeratins in exhaled breath condensate of mechanical ventilated patients. Respir Med, 2007-11-08;102(2):299-306. 2007-11-08 [PMID: 17996434] (Human)
• Zinner RG, Nemunaitis J, Eiseman I, Shin HJ, Olson SC, Christensen J, Huang X, Lenehan PF, Donato NJ, Shin DM Phase I clinical and pharmacodynamic evaluation of oral CI-1033 in patients with refractory cancer. Clin. Cancer Res., 2007-05-15;13(10):3006-14. 2007-05-15 [PMID: 17505003] (Human)
• Fichorova RN, Trifonova RT, Gilbert RO, Costello CE, Hayes GR, Lucas JJ, Singh BN Trichomonas vaginalis lipophosphoglycan triggers a selective upregulation of cytokines by human female reproductive tract epithelial cells. Infect. Immun., 2006-10-01;74(10):5773-9. 2006-10-01 [PMID: 16988255] (Human)
• Leitner JM, Firbas C, Mayr FB, Reiter RA, Steinlechner B, Jilma B Recombinant human antithrombin inhibits thrombin formation and interleukin 6 release in human endotoxemia. Clin. Pharmacol. Ther., 2006-01-01;79(1):23-34. 2006-01-01 [PMID: 16413239] (Human)
• Niessner A, Richter B, Penka M, Steiner S, Strasser B, Ziegler S, Heeb-Elze E, Zorn G, Leitner-Heinschink A, Niessner C, Wojta J, Huber K Endurance training reduces circulating inflammatory markers in persons at risk of coronary events: impact on plaque stabilization? Atherosclerosis, 2005-08-09;186(1):160-5. 2005-08-09 [PMID: 16084517] (Human)
• Watt G, Kantipong P, Jongsakul K, de Souza M, Burnouf T Passive transfer of scrub typhus plasma to patients with AIDS: a descriptive clinical study. QJM, 2001-11-01;94(11):599-607. 2001-11-01 [PMID: 11704689] (Human)

Bioinformatics

• Gene Symbol: CXCL8