Human IL-8/CXCL8 Quantikine ELISA Kit

RT-qPCR for a set of cytokines (CSF2, IL6, IL8 and IL1B) and chemokines (CXCL1 and CXCL6) of total RNA samples derived from mono- and co-cultures in a transwell assay.The respective co- (+) or mono- (-) culture is indicated on the X-axis. The respective analyzed cytokine or chemokine is indicated in the header of each graph. Expression values are shown in arbitrary units (AU) and have been normalized to beta-2 microglobulin (B2M) mRNA copies. Experiments were performed in triplicates (n = 3). Statistical analysis was performed on the mean values by unpaired comparison of mono-cultured HDF and co-cultured HDF RNA samples by using Student’s t-test (**p<0.01, ***p<0.001; n.s.: not significant). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25919140), licensed under a CC-BY license. Not internally tested by R&D Systems.

Pathogen-associated molecular patterns (PAMPs) and damage-associated molecular pattern (DAMPs) induce cytokine expression in human breast cancer cells in vitro. a Relative expression of IL-6 and IL-8 mRNAs after 6 h stimulation with lipopolysaccharide 1 (LPS1) and LPS2, using quantitative real-time PCR (QPCR) on mRNA from the cell lines indicated; n = 6. Error bars standard error of the mean (SEM); ***P <0.001 (analysis of variance (ANOVA)). b Release of IL-6 and IL-8 after stimulation with LPS1 and cycloheximide for 6 h, using ELISA on supernatants from stimulated MDA-MB-231 cells; n = 3. c-e Relative release of IL-6 and IL-8 after stimulation with LPS1, LPS2, the DAMP HMGB1 or IL-1 beta for 6 h, using ELISA on supernatants from stimulated MDA-MB-231 cells (c), SUM-149 cells (d) and SUM-159 cells (e); n = 10. Error bars SEM; *P <0.05, ***P <0.001 (ANOVA). f Dual luciferase reporter assays of MDA-MB-231 cells transfected with an NF kappa B reporter. TK-Renilla was co-transfected as control (Ctrl). LPS1, LPS2, HMGB1 or IL-1 beta was added to stimulate NF kappa B activity as described in “Methods”; n = 14. Error bars SEM; ***P <0.001 (ANOVA). g The relative release of IL-6 and IL-8 after stimulation with the DAMP S100A9 for 20 h, using ELISA on supernatants from stimulated MDA-MB-231, SUM-149 and SUM-159 cells; n = 6. Error bars SEM. *P <0.05, ***P <0.001 (ANOVA). h Transient transfection of pDUO-MD2/hTLR4 for 72 h in MCF-7 cells induces release of both IL-6 and IL-8 measured by ELISA; n = 6. Error bars SEM; *P <0.05, ***P <0.001 (Students t test) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26392082), licensed under a CC-BY license. Not internally tested by R&D Systems.

Toll-like receptor 4 (TLR4) silencing decreases endogenous levels of pro-inflammatory cytokines. a Effect of TLR2/4 silencing in breast cancer cells transfected with negative control (nc) siRNA, or siRNA directed against TLR2 mRNA (si#1 and si#2) or TLR4 mRNA (si#1 and si#2) was analyzed using quantitative real-time PCR; ***P <0.001 (analysis of variance (ANOVA)). b IL-6 (left) and IL-8 (right) ELISA on supernatants from MDA-MB-231 breast cancer cells transfected with nc siRNA, or siRNA directed against TLR2 mRNA (si#1 and si#2) or TLR4 mRNA (si#1 and si#2); n = 4. Error bars standard error of the mean (SEM); *P <0.05, **P <0.01, ***P <0.001 (ANOVA). c Boyden chamber migration assays. Migration of primary human myeloid cells towards supernatants from different cell lines indicated. Human primary peripheral blood mononuclear cells were isolated as previously described [48] and allowed to migrate through a Costar Transwell® Permeable Support 8.0-μm 24-well plate (Corning) to the supernatants of breast cancer supernatants cultured under serum-free conditions. Percentage of migrated CD11b+ cells was analyzed using a flow cytometer and CD11b-APC antibodies (BD Sciences); n = 4. Error bars SEM; *P <0.05, **P <0.01, ***P <0.001 (ANOVA). d Matrigel invasion assays. Invasion of lipopolysaccharide (LPS)-stimulated/un-stimulated MDA-MB-231 cells into matrigel invasion chambers (BD Sciences) as indicated: 25 × 103 MDA-MB-231 cells were stimulated or not with LPS and allowed to invade from 72 h. Amount of invaded cells was analyzed using crystal violet staining and manual counting in four separate experiments; n = 4. Error bars SEM; *P <0.05, **P <0.01, ***P <0.001 (Student’s t test) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26392082), licensed under a CC-BY license. Not internally tested by R&D Systems.

Pathogen-associated molecular patterns (PAMPs) and damage-associated molecular pattern (DAMPs) induce cytokine expression in human breast cancer cells in vitro. a Relative expression of IL-6 and IL-8 mRNAs after 6 h stimulation with lipopolysaccharide 1 (LPS1) and LPS2, using quantitative real-time PCR (QPCR) on mRNA from the cell lines indicated; n = 6. Error bars standard error of the mean (SEM); ***P <0.001 (analysis of variance (ANOVA)). b Release of IL-6 and IL-8 after stimulation with LPS1 and cycloheximide for 6 h, using ELISA on supernatants from stimulated MDA-MB-231 cells; n = 3. c-e Relative release of IL-6 and IL-8 after stimulation with LPS1, LPS2, the DAMP HMGB1 or IL-1 beta for 6 h, using ELISA on supernatants from stimulated MDA-MB-231 cells (c), SUM-149 cells (d) and SUM-159 cells (e); n = 10. Error bars SEM; *P <0.05, ***P <0.001 (ANOVA). f Dual luciferase reporter assays of MDA-MB-231 cells transfected with an NF kappa B reporter. TK-Renilla was co-transfected as control (Ctrl). LPS1, LPS2, HMGB1 or IL-1 beta was added to stimulate NF kappa B activity as described in “Methods”; n = 14. Error bars SEM; ***P <0.001 (ANOVA). g The relative release of IL-6 and IL-8 after stimulation with the DAMP S100A9 for 20 h, using ELISA on supernatants from stimulated MDA-MB-231, SUM-149 and SUM-159 cells; n = 6. Error bars SEM. *P <0.05, ***P <0.001 (ANOVA). h Transient transfection of pDUO-MD2/hTLR4 for 72 h in MCF-7 cells induces release of both IL-6 and IL-8 measured by ELISA; n = 6. Error bars SEM; *P <0.05, ***P <0.001 (Students t test) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26392082), licensed under a CC-BY license. Not internally tested by R&D Systems.

Pathogen-associated molecular patterns (PAMPs) and damage-associated molecular pattern (DAMPs) induce cytokine expression in human breast cancer cells in vitro. a Relative expression of IL-6 and IL-8 mRNAs after 6 h stimulation with lipopolysaccharide 1 (LPS1) and LPS2, using quantitative real-time PCR (QPCR) on mRNA from the cell lines indicated; n = 6. Error bars standard error of the mean (SEM); ***P <0.001 (analysis of variance (ANOVA)). b Release of IL-6 and IL-8 after stimulation with LPS1 and cycloheximide for 6 h, using ELISA on supernatants from stimulated MDA-MB-231 cells; n = 3. c-e Relative release of IL-6 and IL-8 after stimulation with LPS1, LPS2, the DAMP HMGB1 or IL-1 beta for 6 h, using ELISA on supernatants from stimulated MDA-MB-231 cells (c), SUM-149 cells (d) and SUM-159 cells (e); n = 10. Error bars SEM; *P <0.05, ***P <0.001 (ANOVA). f Dual luciferase reporter assays of MDA-MB-231 cells transfected with an NF kappa B reporter. TK-Renilla was co-transfected as control (Ctrl). LPS1, LPS2, HMGB1 or IL-1 beta was added to stimulate NF kappa B activity as described in “Methods”; n = 14. Error bars SEM; ***P <0.001 (ANOVA). g The relative release of IL-6 and IL-8 after stimulation with the DAMP S100A9 for 20 h, using ELISA on supernatants from stimulated MDA-MB-231, SUM-149 and SUM-159 cells; n = 6. Error bars SEM. *P <0.05, ***P <0.001 (ANOVA). h Transient transfection of pDUO-MD2/hTLR4 for 72 h in MCF-7 cells induces release of both IL-6 and IL-8 measured by ELISA; n = 6. Error bars SEM; *P <0.05, ***P <0.001 (Students t test) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26392082), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-8/CXCL8 Quantikine ELISA Kit Summary

• Background: The Quantikine Human IL-8 Immunoassay is a 3.5 hour solid phase ELISA designed to measure human IL-8 in cell culture supernates, serum, and plasma. It is based on antibodies raised against the 72 aa variant of human IL-8 derived from E. coli. It is calibrated with the same recombinant factor. This immunoassay accurately quantitates recombinant human IL-8. Measurement of natural huma...n IL-8 or the 77 aa variant of human IL-8 gave results parallel to the standard curves obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-8.
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• Specificity: Natural and recombinant human IL-8
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CXCL8

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-8/CXCL8 Quantikine ELISA Kit

• 3-10C
• AMCF-I
• C-X-C motif chemokine 8
• CXCL8
• CXCL8SCYB8
• Emoctakin
• GCP1
• GCP-1TSG-1
• IL8
• IL-8
• interleukin 8
• K60
• LAI
• LECT
• LUCT
• LYNAP
• MDNCF
• MDNCFb-ENAP
• member 8
• MONAP
• MONAPGCP1
• NAF
• NAP1
• NAP-1NAP1
• NCF
• Neutrophil-activating protein 1
• Protein 3-10C
• T cell chemotactic factor
• T-cell chemotactic factor
• TCF
• TSG1

Background

Interleukin-8 (IL-8), also known as IL-8, GCP-1, and NAP-1, is a heparin-binding 8-9 kDa member of the alpha, or CXC family of chemokines. There are at least 15 human CXC family members that all adopt a three beta -sheet/one alpha -helix structure. Most CXC chemokines show an N-terminal Glu-Leu-Arg (ELR) tripeptide motif. IL-8 circulates as a monomer, homodimer, and heterodimer with CXCL4/PF4. The monomer is considered the most bio-active, while the heterodimer can potentiate PF4 activity. IL-8 oligomerization is modulated by its interactions with matrix and cell surface glycosaminoglycans (GAGs). Mature human IL-8 shares 65-69% amino acid (aa) identity with canine, feline, and porcine IL-8. There is no IL-8 gene counterpart in rodent. 

Multiple isoforms of IL-8 are generated through both alternative splicing and differential proteolytic cleavage. In humans, alternative splicing generates an iso-form with an eleven aa substitution at the C-terminus. Proteolytic processing results in N-terminal truncation of IL-8 and is likely a cell-specific event. For example, fibroblasts and endothelial cells generate the 1-77 form by cleaving IL-8 following Glu21, while monocytes and lymphocytes generate the 6-77 form by cleaving following Leu25. These truncated forms generally show increased bioactivity, particularly through the CXCR1 receptor. IL-8 can also undergo citrullination on Arg27 of the precursor, a modification that increases its half-life and ability to induce leukocytosis. A wide variety of cells secrete IL-8 including monocytes and neutrophils, fibroblasts and keratinocytes, mast cells, visceral smooth muscle cells, dendritic cells, type II great alveolar cells, and endothelial cells. 

IL-8 bioactivity is mediated through two G-protein-coupled receptors, termed CXCR1/IL-8 RA and CXCR2/IL-8 RB. CXCR1 is 45-50 kDa in size and is used almost exclusively by IL-8. CXCR2 is 35-40 kDa in size and is used by nearly all CXC chemokines. Both CXCR1 and CXCR2 constitutively associate into functional homodimers. They can also heterodimerize, but these complexes dissociate following IL-8 binding. CXCR2 responds to low concentrations of IL-8 and is principally associated with chemotaxis and MMP-9 release. CXCR1, in contrast, responds to high concentrations of IL-8 and is associated with respiratory burst and phospholipase D2 activation. Thus, CXCR2 ligation induces leukocyte adhesion to activated vascular endothelium and migration to sites of inflammation, while CXCR1 ligation primes neutrophil antimicrobial activity. IL-8 can also form a complex with Serpin A1/alpha-1 Antitrypsin, and this prevents IL-8 interaction with CXCR1. 

In addition to its pro-inflammatory effects, IL-8 is involved in angiogenesis and the pathogenesis of atherosclerosis and cancer. It induces VEGF expression in vascular endothelial cells and functions as an autocrine factor for EC growth and angiogenesis. It is upregulated in atherosclerotic lesions and is elevated in the serum and cerebrospinal fluid following myocardial infarction. In cancer, IL-8 promotes epithelial-mesenchymal transition as well as tumor cell invasiveness and metastasis.

&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CXCL8/IL-8 (D8000C)(449)

Publications using D8000C

• Kido, R;Hiroshima, Y;Kido, JI;Bando, M;Yoshida, K;Inagaki, Y;Yumoto, H; Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1? in human neutrophil-like cells Journal of oral biosciences 2025-01-30 [PMID: 39892784] (Human)
• Kowalska, M;Tylicka, M;Koper-Lenkiewicz, OM;Kami?ska, J;Dorf, J;Matuszczak, E; Plasma concentration of MMP-17 is elevated in boys with cryptorchidism and correlates with HSP-70 Scientific reports 2025-01-02 [PMID: 39747260] (Human)
• Panda, K;Santiago, MJ;Rahman, MS;Ghorai, S;Black, SM;Rahman, I;Unwalla, HJ;Chinnapaiyan, S; HIV-1 Tat Protein and Cigarette Smoke Mediated ADAM17 Upregulation Can Lead to Impaired Mucociliary Clearance Cells 2024-12-05 [PMID: 39682757] (Human)
• Alshehri, JH;Al-Nasrallah, HK;Al-Ansari, MM;Aboussekhra, A; Activation of Mammary Epithelial and Stromal Fibroblasts upon Exposure to Escherichia coli Metabolites Cells 2024-10-17 [PMID: 39451241] (Human)
• Saraf, N;Ramachandran, RA;Cao, M;Lemoff, A;Baniasadi, H;Robertson, DM; Serum-Derived Extracellular Vesicles for the Treatment of Severe Ocular Surface Disease The ocular surface 2024-08-17 [PMID: 39159888] (Human)
• Polis, B;Zeman, K;Zakrzewski, K;Fabijan, A;Nowos?awska, E; Peripheral white blood cell patterns in children with hydrocephalus as a response to ventriculo-peritoneal shunt infection PloS one 2024-08-09 [PMID: 39121090] (Human)
• Matsui, C;Koide, H;Ikeda, T;Ikegami, T;Yamamoto, T;Escandón, JM;Mohammad, A;Ito, T;Mizuno, H; Cytokines released from human adipose tissue-derived stem cells by bFGF stimulation: Effects of IL-8 and CXCL-1 on wound healing Regenerative therapy 2024-06-01 [PMID: 39045577] (Human)
• Widjaja, AA;Lim, WW;Viswanathan, S;Chothani, S;Corden, B;Dasan, CM;Goh, JWT;Lim, R;Singh, BK;Tan, J;Pua, CJ;Lim, SY;Adami, E;Schafer, S;George, BL;Sweeney, M;Xie, C;Tripathi, M;Sims, NA;Hübner, N;Petretto, E;Withers, DJ;Ho, L;Gil, J;Carling, D;Cook, SA; Inhibition of IL-11 signalling extends mammalian healthspan and lifespan Nature 2024-07-17 [PMID: 39020175] (Human)
• Peng, Z;Zhang, W;Hong, H;Liu, L; Effect of luteolin on oxidative stress and inflammation in the human osteoblast cell line hFOB1.19 in an inflammatory microenvironment BMC pharmacology & toxicology 2024-07-12 [PMID: 38997762] (Human)
• Hu, Q;Qu, W;Zhang, T;Feng, J;Dong, X;Nie, R;Chen, J;Wang, X;Peng, C;Ke, X; C1q/Tumor Necrosis Factor-Related Protein-9 Is a Novel Vasculoprotective Cytokine That Restores High Glucose-Suppressed Endothelial Progenitor Cell Functions by Activating the Endothelial Nitric Oxide Synthase Journal of the American Heart Association 2024-02-20 [PMID: 38348774] (Human)
• Flores-Pliego, A;Espejel-Nuñez, A;Borboa-Olivares, H;Parra-Hernández, SB;Montoya-Estrada, A;González-Márquez, H;González-Camarena, R;Estrada-Gutierrez, G; Regulation of MMP-2 by IL-8 in Vascular Endothelial Cells: Probable Mechanism for Endothelial Dysfunction in Women with Preeclampsia International journal of molecular sciences 2023-12-21 [PMID: 38203296] (Human)
• Jin, Y;Zhang, D;Deng, K;Wu, P;Yang, D;Xie, Z;Qiu, W;Yu, G; Role of the cAMP-PKA-NF-?B pathway in Mucin1 over-expression in A549 cells during Respiratory syncytial virus infection BMC infectious diseases 2023-11-30 [PMID: 38036963] (Human)
• Ur Rahim, J;Faheem, MM;Nawaz, S;Goswami, A;Rai, R; Synthesis and characterization of piperic acid conjugates with homochiral and heterochiral dipeptides containing phenylalanine and their application in skin cancer Peptides 2023-11-03 [PMID: 37923167] (Mouse)
• Cho, YE;Kim, Y;Kim, SJ;Lee, H;Hwang, S; Overexpression of Interleukin-8 Promotes the Progression of Fatty Liver to Nonalcoholic Steatohepatitis in Mice International journal of molecular sciences 2023-10-23 [PMID: 37895168] (Human)
• Kami?ska, J;Koper-Lenkiewicz, OM;Ponikwicka-Tyszko, D;Lebiedzi?ska, W;Palak, E;Sztachelska, M;Bernaczyk, P;Dorf, J;Guzi?ska-Ustymowicz, K;Zar?ba, K;Wo?czy?ski, S;Rahman, NA;Dymicka-Piekarska, V; New Insights on the Progesterone (P4) and PGRMC1/NENF Complex Interactions in Colorectal Cancer Progression Cancers 2023-10-20 [PMID: 37894441] (Human)
• Planchais, C;Molinos-Albert, LM;Rosenbaum, P;Hieu, T;Kanyavuz, A;Clermont, D;Prazuck, T;Lefrou, L;Dimitrov, JD;Hüe, S;Hocqueloux, L;Mouquet, H; HIV-1 treatment timing shapes the human intestinal memory B-cell repertoire to commensal bacteria Nature communications 2023-10-10 [PMID: 37816704] (Human)
• Wright, CW;Li, N;Shaffer, L;Hill, A;Boyer, N;Alves, SE;Venkataraman, S;Biswas, K;Lieberman, LA;Mohammadi, S; Establishment of a 96-well transwell system using primary human gut organoids to capture multiple quantitative pathway readouts Scientific reports 2023-09-29 [PMID: 37773535] (Human)
• Kim, HJ;Chang, HK;Lee, YM;Heo, K; Catecholamines Promote Ovarian Cancer Progression through Secretion of CXC-Chemokines International journal of molecular sciences 2023-09-14 [PMID: 37762405] ( Human)
• Aboussekhra, A;Alraouji, NN;Al-Mohanna, FH;Al-Khalaf, H; Ionizing radiation normalizes the features of active breast cancer stromal fibroblasts and suppresses their paracrine pro-carcinogenic effects Translational oncology 2023-09-04 [PMID: 37672859] (Human)
• Roach, DR;Noël, B;Chollet-Martin, S;de Jode, M;Granger, V;Debarbieux, L;de Chaisemartin, L; Human Neutrophil Response to Pseudomonas Bacteriophage PAK_P1, a Therapeutic Candidate Viruses 2023-08-12 [PMID: 37632068] (Human)
• Murase, H;Matsuo, Y;Denda, Y;Nonoyama, K;Kato, T;Aoyama, Y;Hayashi, Y;Imafuji, H;Saito, K;Morimoto, M;Ogawa, R;Takahashi, H;Mitsui, A;Kimura, M;Takiguchi, S; Upregulation of integrin‑linked kinase enhances tumor progression in gemcitabine‑resistant pancreatic cancer Oncology reports 2023-09-01 [PMID: 37477162] (Human)
• Gajić, M;Schröder-Heurich, B;Horvat Mercnik, M;Cervar-Zivkovic, M;Wadsack, C;von Versen-Höynck, F;Mayer-Pickel, K; The Impact of Hydroxychloroquine on Primary Feto-Placental Endothelial Cells from Healthy and Early-Onset Preeclamptic Placentas International journal of molecular sciences 2023-06-30 [PMID: 37446111] (Human)
• Flerlage, T;Crawford, JC;Allen, EK;Severns, D;Tan, S;Surman, S;Ridout, G;Novak, T;Randolph, A;West, AN;Thomas, PG; Single cell transcriptomics identifies distinct profiles in pediatric acute respiratory distress syndrome Nature communications 2023-06-30 [PMID: 37391405] (Human)
• Tsukamoto, S;Koma, YI;Kitamura, Y;Tanigawa, K;Azumi, Y;Miyako, S;Urakami, S;Hosono, M;Kodama, T;Nishio, M;Shigeoka, M;Yokozaki, H; Matrix Metalloproteinase 9 Induced in Esophageal Squamous Cell Carcinoma Cells via Close Contact with Tumor-Associated Macrophages Contributes to Cancer Progression and Poor Prognosis Cancers 2023-05-30 [PMID: 37296952] (Human)
• Ryu, Y;Roh, S;Joung, YS; Assessing the cytotoxicity of aerosolized carbon black and benzo[a]pyrene with controlled physical and chemical properties on human lung epithelial cells Scientific reports 2023-06-08 [PMID: 37291179] (Human)
• Fei, X;Huang, J;Li, F;Wang, Y;Shao, Z;Dong, L;Wu, Y;Li, B;Zhang, X;Lv, B;Zhao, Y;Weng, Q;Chen, K;Zhang, M;Yang, S;Zhang, C;Zhang, M;Li, W;Ying, S;Sun, Q;Chen, Z;Shen, H; The Scap-SREBP1-S1P/S2P lipogenesis signal orchestrates the homeostasis and spatiotemporal activation of NF-?B Cell reports 2023-06-01 [PMID: 37267109] (Human)
• Kim, JY;Yi, MH;Kim, M;Choi, JH;Lee, S;Yong, TS; Production of Dermatophagoides farinae Having Low Bacterial Content Using Ampicillin Journal of immunology research 2023-05-18 [PMID: 37252681] (Human)
• Montero, P;Roger, I;Estornut, C;Milara, J;Cortijo, J; Influence of dose and exposition time in the effectiveness of N-Acetyl-l-cysteine treatment in A549 human epithelial cells Heliyon 2023-05-01 [PMID: 37144195] (Human)
• W Alabdullat, A Almnaizel, A Alhijji, A Alshathri, A Albarrag, I Bindayel Correlation of Plasma 25(OH)D3 and Vitamin D Binding Protein Levels with COVID-19 Severity and Outcome in Hospitalized Patients Nutrients, 2023-04-10;15(8):. 2023-04-10 [PMID: 37111039] (Human)
• DY Usupzhanov, TA Astrelina, IV Kobzeva, YB Suchkova, VA Brunchukov, AA Rastorguev, VA Nikitina, AS Samoilov Evaluation of Changes in Some Functional Properties of Human Mesenchymal Stromal Cells Induced by Low Doses of Ionizing Radiation International Journal of Molecular Sciences, 2023-03-28;24(7):. 2023-03-28 [PMID: 37047317] (Human)
• A Alshalani, BM Beuger, AM Tuip-de Bo, R van Brugge, JP Acker, NP Juffermans The impact of biological age of red blood cell on in vitro endothelial activation markers Frontiers in Physiology, 2023-03-08;14(0):1127103. 2023-03-08 [PMID: 36969576] (Human)
• Y Takamatsu, S Hayashi, H Kumamoto, S Imoto, Y Tanaka, H Mitsuya, N Higashi-Ku A novel anti-HBV agent, E-CFCP, restores Hepatitis B virus (HBV)-induced senescence-associated cellular marker perturbation in human hepatocytes Virus research, 2023-03-23;329(0):199094. 2023-03-23 [PMID: 36933835] (Human)
• M Maskey-War, R Rubinsztaj, T Przyby?ows, K Karwat, P Nejman-Gry, M Papli?ska-, R Chazan Serum Amyloid A in Stable Patients with Chronic Obstructive Pulmonary Disease Does Not Reflect the Clinical Course of the Disease International Journal of Molecular Sciences, 2023-01-27;24(3):. 2023-01-27 [PMID: 36768801] (Human)
• EA Ross, LA Turner, H Donnelly, A Saeed, MP Tsimbouri, KV Burgess, G Blackburn, V Jayawarna, Y Xiao, MAG Oliva, J Willis, J Bansal, P Reynolds, JA Wells, J Mountford, M Vassalli, N Gadegaard, ROC Oreffo, M Salmeron-S, MJ Dalby Nanotopography reveals metabolites that maintain the immunomodulatory phenotype of mesenchymal stromal cells Nature Communications, 2023-02-10;14(1):753. 2023-02-10 [PMID: 36765065] (Human)
• T Tsai, M Alwees, MA Asaad, J Theile, V Kakkassery, HB Dick, T Schultz, SC Joachim Increased Angiopoietin-1 and -2 levels in human vitreous are associated with proliferative diabetic retinopathy PLoS ONE, 2023-01-20;18(1):e0280488. 2023-01-20 [PMID: 36662891] (Human)
• F De Luca, M Kha, K Swärd, ME Johansson Identification of ARMH4 and WIPF3 as human podocyte proteins with potential roles in immunomodulation and cytoskeletal dynamics PLoS ONE, 2023-01-17;18(1):e0280270. 2023-01-17 [PMID: 36649229] (Human)
• FP Veras, GA Publio, BM Melo, DS Prado, T Norbiato, NT Cecilio, C Hiroki, LEA Damasceno, R Jung, JE Toller-Kaw, TV Martins, SF Assunção, D Lima, MG Alves, GV Vieira, LA Tavares, ALR Alves-Reze, SH Karbach, HI Nakaya, TM Cunha, CS Souza, FQ Cunha, KU Sales, A Waisman, JC Alves-Filh Pyruvate kinase M2 mediates IL-17 signaling in keratinocytes driving psoriatic skin inflammation Cell Reports, 2022-12-27;41(13):111897. 2022-12-27 [PMID: 36577385] (Human)
• J Wang, G Li, M Lin, S Lin, L Wu microRNA-338-3p suppresses lipopolysaccharide-induced inflammatory response in HK-2 cells BMC molecular and cell biology, 2022-12-23;23(1):60. 2022-12-23 [PMID: 36564725] (Human)
• J Milara, F Martínez-E, P Montero, I Roger, MA Bayarri, P Ribera, MN Oishi-Kona, JR Alba-Garcí, E Zapater, J Cortijo N-acetylcysteine Reduces Inflammasome Activation Induced by SARS-CoV-2 Proteins In Vitro International Journal of Molecular Sciences, 2022-11-22;23(23):. 2022-11-22 [PMID: 36498845] (Human)
• R Dinami, L Pompili, E Petti, M Porru, C D'Angelo, S Di Vito, A Rizzo, V Campani, G De Rosa, A Bruna, V Serra, M Mano, M Giacca, C Leonetti, G Ciliberto, M Tarsounas, A Stoppaccia, S Schoeftner, A Biroccio MiR-182-3p targets TRF2 and impairs tumor growth of triple-negative breast cancer Embo Molecular Medicine, 2022-11-25;0(0):e16033. 2022-11-25 [PMID: 36426578] (Human)
• G Costabile, E Mitidieri, D Visaggio, R Provenzano, A Miro, F Quaglia, I d'Angelo, E Frangipani, R Sorrentino, P Visca, R d'Emmanuel, F Ungaro Boosting Lung Accumulation Of Gallium With Inhalable Nano-Embedded Microparticles For The Treatment Of Bacterial Pneumonia International journal of pharmaceutics, 2022-11-13;0(0):122400. 2022-11-13 [PMID: 36384182] (Rat)
• K Li, Y Zhu, P Zhang, M Alini, S Grad, Z Li Anti-inflammatory and pro-anabolic effects of 5-aminosalicylic acid on human inflammatory osteoarthritis models Journal of Orthopaedic Translation, 2022-10-29;38(0):106-116. 2022-10-29 [PMID: 36381242] (Human)
• S Cadau, M Gault, N Berthelemy, CY Hsu, L Danoux, N Pelletier, D Goudounèch, C Pons, C Leprince, V André-Frei, M Simon, S Pain An Inflamed and Infected Reconstructed Human Epidermis to Study Atopic Dermatitis and Skin Care Ingredients International Journal of Molecular Sciences, 2022-10-25;23(21):. 2022-10-25 [PMID: 36361668] (Human)
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• G Rodionov, M Rosenzwaig, MS Tzadok, M Kvint, E Gevir, E Zorde-Khva, A Peled, S Yarkoni, A Ofer Short treatment of peripheral blood cells product with Fas ligand using closed automated cell processing system significantly reduces immune cell reactivity of the graft in vitro and in vivo Bone marrow transplantation, 2022-05-10;0(0):. 2022-05-10 [PMID: 35538142] (Human)
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• YH Lee, SJ Lin Chitosan/PVA Hetero-Composite Hydrogel Containing Antimicrobials, Perfluorocarbon Nanoemulsions, and Growth Factor-Loaded Nanoparticles as a Multifunctional Dressing for Diabetic Wound Healing: Synthesis, Characterization, and In Vitro/In Vivo Evaluation Pharmaceutics, 2022-02-28;14(3):. 2022-02-28 [PMID: 35335913] (Human)
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• HS Kim, JW Lim, H Kim Korean Red Ginseng Extract Inhibits IL-8 Expression via Nrf2 Activation in Helicobacter pylori-Infected Gastric Epithelial Cells Nutrients, 2022-02-28;14(5):. 2022-02-28 [PMID: 35268019] (Human)
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• P Montero, J Milara, M Pérez-Leal, C Estornut, I Roger, A Pérez-Fida, C Sanz, J Cortijo Paclitaxel-Induced Epidermal Alterations: An In Vitro Preclinical Assessment in Primary Keratinocytes and in a 3D Epidermis Model International Journal of Molecular Sciences, 2022-01-20;23(3):. 2022-01-20 [PMID: 35163066] (Human)
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• HL Wang, FQ Chen, LJ Wu Ephedrine ameliorates chronic obstructive pulmonary disease (COPD) through restraining endoplasmic reticulum (ER) stress in vitro and in vivo International immunopharmacology, 2021-12-22;103(0):107842. 2021-12-22 [PMID: 34953449] (Human)
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• W Panpetch, P Visitchana, W Saisorn, A Sawatpanic, P Chatthanat, N Somboonna, S Tumwasorn, A Leelahavan Lactobacillus rhamnosus attenuates Thai chili extracts induced gut inflammation and dysbiosis despite capsaicin bactericidal effect against the probiotics, a possible toxicity of high dose capsaicin PLoS ONE, 2021-12-23;16(12):e0261189. 2021-12-23 [PMID: 34941893] (Human)
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• VV Edara, S Nooka, J Proulx, S Stacy, A Ghorpade, K Borgmann &amp;beta-Catenin Regulates Wound Healing and IL-6 Expression in Activated Human Astrocytes Biomedicines, 2020-11-06;8(11):. 2020-11-06 [PMID: 33171974] (Human)
• T Samejima, T Nagamatsu, N Akiba, T Fujii, S Sayama, K Kawana, A Taguchi, K Kumasawa, T Iriyama, Y Osuga, T Fujii Secretory leukocyte protease inhibitor and progranulin as possible regulators of cervical remodeling in pregnancy J Reprod Immunol, 2020-11-01;143(0):103241. 2020-11-01 [PMID: 33157500] (Human)
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• R Li, Y Li, X Dong Preconditioning mesenchymal stromal cells with flagellin enhances the anti?inflammatory ability of their secretome against lipopolysaccharide?induced acute lung injury Molecular Medicine Reports, 2020-07-28;0(0):. 2020-07-28 [PMID: 32945411] (Human)
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• Estrogen’s bone-protective effects may involve differential IL-1 receptor regulation in human osteoclast-like cells [PMID: 10330423] (Human)
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• Lehner T, —îÊëÖ“§, Wang Y, r¹oŽ&A½ýx, Cranage M, Ïï§Ù´âoÑ‘, Bergmeier LA, Mitchell E, Tao L, Hall G, Dennis M, Cook N, Brookes R, Klavinskis L, Jones I, Doyle C, Ward R Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques. 1996-07-01;2(7):767-75. 1996-07-01 [PMID: 8673922] (Primate - Macaca mulatta (Rhesus Macaque))

Bioinformatics

• Gene Symbol: CXCL8