Human IL-8/CXCL8 Quantikine HS ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-8/CXCL8 Quantikine HS ELISA Kit Summary

• Background: The Quantikine HS Human IL-8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-8 levels in serum and plasma. It contains E. coli-expressed recombinant human IL-8 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-8 accurately. Results obtained using natural human IL-8 showed linear curves that were paralle...l to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-8.
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• Specificity: Natural and recombinant human IL-8
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CXCL8

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-8/CXCL8 Quantikine HS ELISA Kit

• 3-10C
• AMCF-I
• C-X-C motif chemokine 8
• CXCL8
• CXCL8SCYB8
• Emoctakin
• GCP1
• GCP-1TSG-1
• IL8
• IL-8
• interleukin 8
• K60
• LAI
• LECT
• LUCT
• LYNAP
• MDNCF
• MDNCFb-ENAP
• member 8
• MONAP
• MONAPGCP1
• NAF
• NAP1
• NAP-1NAP1
• NCF
• Neutrophil-activating protein 1
• Protein 3-10C
• T cell chemotactic factor
• T-cell chemotactic factor
• TCF
• TSG1

Background

Interleukin-8 (IL-8), also known as IL-8, GCP-1, and NAP-1, is a heparin-binding 8-9 kDa member of the alpha, or CXC family of chemokines. There are at least 15 human CXC family members that all adopt a three beta -sheet/one alpha -helix structure. Most CXC chemokines show an N-terminal Glu-Leu-Arg (ELR) tripeptide motif. IL-8 circulates as a monomer, homodimer, and heterodimer with CXCL4/PF4. The monomer is considered the most bio-active, while the heterodimer can potentiate PF4 activity. IL-8 oligomerization is modulated by its interactions with matrix and cell surface glycosaminoglycans (GAGs). Mature human IL-8 shares 65-69% amino acid (aa) identity with canine, feline, and porcine IL-8. There is no IL-8 gene counterpart in rodent. 

Multiple isoforms of IL-8 are generated through both alternative splicing and differential proteolytic cleavage. In humans, alternative splicing generates an iso-form with an eleven aa substitution at the C-terminus. Proteolytic processing results in N-terminal truncation of IL-8 and is likely a cell-specific event. For example, fibroblasts and endothelial cells generate the 1-77 form by cleaving IL-8 following Glu21, while monocytes and lymphocytes generate the 6-77 form by cleaving following Leu25. These truncated forms generally show increased bioactivity, particularly through the CXCR1 receptor. IL-8 can also undergo citrullination on Arg27 of the precursor, a modification that increases its half-life and ability to induce leukocytosis. A wide variety of cells secrete IL-8 including monocytes and neutrophils, fibroblasts and keratinocytes, mast cells, visceral smooth muscle cells, dendritic cells, type II great alveolar cells, and endothelial cells. 

IL-8 bioactivity is mediated through two G-protein-coupled receptors, termed CXCR1/IL-8 RA and CXCR2/IL-8 RB. CXCR1 is 45-50 kDa in size and is used almost exclusively by IL-8. CXCR2 is 35-40 kDa in size and is used by nearly all CXC chemokines. Both CXCR1 and CXCR2 constitutively associate into functional homodimers. They can also heterodimerize, but these complexes dissociate following IL-8 binding. CXCR2 responds to low concentrations of IL-8 and is principally associated with chemotaxis and MMP-9 release. CXCR1, in contrast, responds to high concentrations of IL-8 and is associated with respiratory burst and phospholipase D2 activation. Thus, CXCR2 ligation induces leukocyte adhesion to activated vascular endothelium and migration to sites of inflammation, while CXCR1 ligation primes neutrophil antimicrobial activity. IL-8 can also form a complex with Serpin A1/alpha-1 Antitrypsin, and this prevents IL-8 interaction with CXCR1. 

In addition to its pro-inflammatory effects, IL-8 is involved in angiogenesis and the pathogenesis of atherosclerosis and cancer. It induces VEGF expression in vascular endothelial cells and functions as an autocrine factor for EC growth and angiogenesis. It is upregulated in atherosclerotic lesions and is elevated in the serum and cerebrospinal fluid following myocardial infarction. In cancer, IL-8 promotes epithelial-mesenchymal transition as well as tumor cell invasiveness and metastasis.

Publications for CXCL8/IL-8 (HS800)(37)

Publications using HS800

• Koutaki, D;Paltoglou, G;Manou, M;Vourdoumpa, A;Ramouzi, E;Tzounakou, AM;Michos, A;Bacopoulou, F;Mantzou, E;Zoumakis, E;Papadopoulou, M;Kassari, P;Charmandari, E; The Role of Secreted Frizzled-Related Protein 5 (Sfrp5) in Overweight and Obesity in Childhood and Adolescence Nutrients 2024-09-17 [PMID: 39339733] (Human)
• Dunleavy, C;Elsworthy, RJ;Wood, SJ;Allott, K;Spencer, F;Upthegrove, R;Aldred, S; Exercise4Psychosis: A randomised control trial assessing the effect of moderate-to-vigorous exercise on inflammatory biomarkers and negative symptom profiles in men with first-episode psychosis Brain, behavior, and immunity 2024-06-19 [PMID: 38906488] (Human)
• Pike, MR;Lipner, E;O'Brien, KJ;Breen, EC;Cohn, BA;Cirillo, PM;Krigbaum, NY;Kring, AM;Olino, TM;Alloy, LB;Ellman, LM; Prenatal maternal Inflammation, childhood cognition and adolescent depressive symptoms Brain, behavior, and immunity 2024-05-16 [PMID: 38761818] (Human)
• Niemelä, O;Bloigu, A;Bloigu, R;Nivukoski, U;Kultti, J;Pohjasniemi, H; Patterns of IgA Autoantibody Generation, Inflammatory Responses and Extracellular Matrix Metabolism in Patients with Alcohol Use Disorder International journal of molecular sciences 2023-08-23 [PMID: 37685930] (Human)
• Koziej, P;Kluszczynska, K;Hartman, ML;Czyz, M; Trametinib-Resistant Melanoma Cells Displaying MITFhigh/NGFRlow/IL-8low Phenotype Are Highly Responsive to Alternating Periods of Drug Withdrawal and Drug Rechallenge International journal of molecular sciences 2023-04-26 [PMID: 37175614] (Human)
• R De Marco, AW Barritt, M Cercignani, G Cabbai, A Colasanti, NA Harrison Inflammation-induced reorientation of reward versus punishment sensitivity is attenuated by Minocycline Brain, Behavior, and Immunity, 2023-04-25;0(0):. 2023-04-25 [PMID: 37105388] (Human)
• RM Critelli, F Milosa, A Romanzi, S Lasagni, G Marcelli, L Di Marco, A Pivetti, F Schepis, D Romagnoli, S Mancarella, F Dituri, ML Martinez-C, G Giannelli, E Villa Upregulation of the oestrogen target gene SIX1 is associated with higher growth speed and decreased survival in HCV-positive women with hepatocellular carcinoma Oncology Letters, 2022-09-21;24(5):395. 2022-09-21 [PMID: 36276500] (Human)
• RJ Pawlik, L Petrakova, L Brotte, H Engler, S Benson, S Elsenbruch Circulating Pro-inflammatory Cytokines Do Not Explain Interindividual Variability in Visceral Sensitivity in Healthy Individuals Frontiers in Neuroscience, 2022-07-04;16(0):876490. 2022-07-04 [PMID: 35860299] (Human)
• Z Chen, J Zhu, H Zhou, Y Jia, H Ruan, Q Diao, M Li, L Zheng, S Yao, Y Guo, Y Zhou, Y Jiang The involvement of copper, circular RNAs, and inflammatory cytokines in chronic respiratory disease Chemosphere, 2022-05-20;303(0):135005. 2022-05-20 [PMID: 35605724] (Human)
• Y Du, K Yang, Z Zhou, L Wu, L Wang, Y Chen, X Ge, X Wang Nicotine regulates autophagy of human periodontal ligament cells through alpha7 nAchR that promotes secretion of inflammatory factors IL-1beta and IL-8 BMC Oral Health, 2021-11-03;21(1):560. 2021-11-03 [PMID: 34732192] (Human)
• M Yamaya, X Deng, A Kikuchi, M Sugawara, N Saito, T Kubo, H Momma, T Kawase, K Nakagome, Y Shimotai, H Nishimura The proton ATPase inhibitor bafilomycin A1 reduces the release of rhinovirus C and cytokines from primary cultures of human nasal epithelial cells Virus research, 2021-08-21;304(0):198548. 2021-08-21 [PMID: 34425162] (Human)
• M Jamka, P Bogda?ski, P Krzy?anows, A Mi?kiewicz, J Karolkiewi, M Du?-?uchow, R M?dry, A Lisowska, A Gotz-Wi?ck, S Iskakova, J Walkowiak, E M?dry Endurance Training Depletes Antioxidant System but Does Not Affect Endothelial Functions in Women with Abdominal Obesity: A Randomized Trial with a Comparison to Endurance-Strength Training Journal of Clinical Medicine, 2021-04-12;10(8):. 2021-04-12 [PMID: 33921520] (Human)
• MH Hanindita, R Irawan, IDG Ugrasena, IGBA Hariastawa Comparison of two lipid emulsions on interleukin-1&beta, interleukin-8 and fatty acid composition in infants post gastrointestinal surgery:�a randomized trial F1000Research, 2020-09-24;9(0):1168. 2020-09-24 [PMID: 33299555] (Human)
• K Jaskiewicz, K Mycroft, M Maskey-War, K Paralusz, N Siemiez, P Nejman-Gry, M Barnas, R Krenke, K Gorska Exhaled Biomarkers in Idiopathic Pulmonary Fibrosis-A Six-Month Follow-Up Study in Patients Treated with Pirfenidone J Clin Med, 2020-08-05;9(8):. 2020-08-05 [PMID: 32764328] (Human)
• T Reichel, TK Bo beta lau, J Palmowski, K Eder, R Ringseis, FC Mooren, R Walscheid, E Bothur, S Samel, T Frech, M Philippe, K Krüger Reliability and suitability of physiological exercise response and recovery markers Sci Rep, 2020-07-17;10(1):11924. 2020-07-17 [PMID: 32681124] (Human)
• E Schneck, F Edinger, M Hecker, N Sommer, O Pak, N Weissmann, A Hecker, M Reichert, M Markmann, M Sander, C Koch Blood Levels of Free-Circulating Mitochondrial DNA in Septic Shock and Postsurgical Systemic Inflammation and Its Influence on Coagulation: A Secondary Analysis of a Prospective Observational Study J Clin Med, 2020-06-30;9(7):. 2020-06-30 [PMID: 32629885] (Human)
• E Zuccolo, I Badi, F Scavello, I Gambuzza, L Mancinelli, F Macrì, CC Tedesco, F Veglia, AR Bonfigli, F Olivieri, A Raucci The microRNA-34a-Induced Senescence-Associated Secretory Phenotype (SASP) Favors Vascular Smooth Muscle Cells Calcification Int J Mol Sci, 2020-06-23;21(12):. 2020-06-23 [PMID: 32585876] (Human)
• ML Hartman, M Rogut, A Mielczarek, M Wozniak, M Czyz 17-Aminogeldanamycin Inhibits Constitutive Nuclear Factor-Kappa B (NF-kappaB) Activity in Patient-Derived Melanoma Cell Lines Int J Mol Sci, 2020-05-26;21(11):. 2020-05-26 [PMID: 32466509] (Human)
• P Fatyga, A Pac, M Fedyk-?uka, T Grodzicki, A Skalska The relationship between malnutrition risk and inflammatory biomarkers in outpatient geriatric population Eur Geriatr Med, 2020-03-06;0(0):. 2020-03-06 [PMID: 32297262] (Human)
• AN Burska, PV Giannoudis, BH Tan, D Ilas, E Jones, F Ponchel Dynamics of Early Signalling Events during Fracture Healing and Potential Serum Biomarkers of Fracture Non-Union in Humans J Clin Med, 2020-02-11;9(2):. 2020-02-11 [PMID: 32054088] (Human)
• G Merckx, B Hosseinkha, S Kuypers, S Deville, J Irobi, I Nelissen, L Michiels, I Lambrichts, A Bronckaers Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles Cells, 2020-01-28;9(2):. 2020-01-28 [PMID: 32012900] (Human)
• M Auclair, AC Guénantin, S Fellahi, M Garcia, J Capeau HIV antiretroviral drugs, dolutegravir, maraviroc and ritonavir-boosted atazanavir use different pathways to affect inflammation, senescence and insulin sensitivity in human coronary endothelial cells PLoS ONE, 2020-01-23;15(1):e0226924. 2020-01-23 [PMID: 31971958] (Human)
• J Doll, A Moghaddam, V Daniel, B Biglari, R Heller, G Schmidmaie, TF Raven LIPUS vs. reaming in non-union treatment: Cytokine expression course as a tool for evaluation and differentiation of non-union therapy J Orthop, 2019-08-13;17(0):208-214. 2019-08-13 [PMID: 31889743] (Human)
• N Aziz, R Detels, JJ Quint, D Gjertson, T Ryner, AW Butch Biological variation of immunological blood biomarkers in healthy individuals and quality goals for biomarker tests BMC Immunol., 2019-09-14;20(1):33. 2019-09-14 [PMID: 31521107] (Human)
• E Krock, M Millecamps, KM Anderson, A Srivastava, TE Reihsen, P Hari, YR Sun, SH Jang, GL Wilcox, KG Belani, DS Beebe, J Ouellet, MR Pinto, LJ Kehl, L Haglund, LS Stone Interleukin-8 as a therapeutic target for chronic low back pain: Upregulation in human cerebrospinal fluid and pre-clinical validation with chronic reparixin in the SPARC-null mouse model EBioMedicine, 2019-04-30;0(0):. 2019-04-30 [PMID: 31047862] (Human)
• E Kaasinen, O Kuismin, K Rajamäki, H Ristolaine, M Aavikko, J Kondelin, S Saarinen, DG Berta, R Katainen, EAM Hirvonen, A Karhu, A Taira, T Tanskanen, A Alkodsi, M Taipale, E Morgunova, K Franssila, R Lehtonen, M Mäkinen, K Aittomäki, A Palotie, MI Kurki, O Pietiläine, M Hilpert, E Saarentaus, J Niinimäki, J Junttila, K Kaikkonen, P Vahteristo, RC Skoda, MRJ Seppänen, KK Eklund, J Taipale, O Kilpivaara, LA Aaltonen Impact of constitutional TET2 haploinsufficiency on molecular and clinical phenotype in humans Nat Commun, 2019-03-19;10(1):1252. 2019-03-19 [PMID: 30890702] (Human)
• C Metzler-Ba, JP Mole, E Leonaviciu, R Sims, EJ Kidd, B Ertefai, A Kelso-Mitc, F Gidney, F Fasano, J Evans, DK Jones, RJ Baddeley Sex-specific effects of central adiposity and inflammatory markers on limbic microstructure Neuroimage, 2019-02-05;189(0):793-803. 2019-02-05 [PMID: 30735826] (Human)
• H Ugge, J Carlsson, B Söderquist, K Fall, O Andén, S Davidsson The influence of prostatic Cutibacterium acnes infection on serum levels of IL6 and CXCL8 in prostate cancer patients Infect. Agents Cancer, 2018-11-14;13(0):34. 2018-11-14 [PMID: 30473726] (Human)
• LA Sjaarda, KA Ahrens, DL Kuhr, TL Holland, UR Omosigho, BT Steffen, NL Weir, HK Tollman, RM Silver, MY Tsai, EF Schisterma Pilot study of placental tissue collection, processing, and measurement procedures for large scale assessment of placental inflammation PLoS ONE, 2018-05-11;13(5):e0197039. 2018-05-11 [PMID: 29750805] (Human)
• R Critelli, F Milosa, F Faillaci, R Condello, E Turola, L Marzi, B Lei, F Dituri, S Andreani, P Sighinolfi, P Manni, A Maiorana, C Caporali, F di Benedet, M Del Buono, N De Maria, F Schepis, ML Martinez-C, G Giannelli, E Villa Microenvironment inflammatory infiltrate drives growth speed and outcome of hepatocellular carcinoma: a prospective clinical study Cell Death Dis, 2017-08-24;8(8):e3017. 2017-08-24 [PMID: 28837142] (Human)
• I Zalesna, M Osrodek, ML Hartman, M Rozanski, M Sztiller-S, K Niewinna, D Nejc, M Czyz Exogenous growth factors bFGF, EGF and HGF do not influence viability and phenotype of V600EBRAF melanoma cells and their response to vemurafenib and trametinib in vitro PLoS ONE, 2017-08-22;12(8):e0183498. 2017-08-22 [PMID: 28829835] (Human)
• E Kenne Sare, LB Mårtensson, BA Andersson, P Karlsson, I Bergh Mindfulness and its efficacy for psychological and biological responses in women with breast cancer Cancer Med, 2017-04-18;0(0):. 2017-04-18 [PMID: 28421677] (Human)
• Singh R, Mackay A, Patel A, Garcha D, Kowlessar B, Brill S, Donnelly L, Barnes P, Donaldson G, Wedzicha J Inflammatory thresholds and the species-specific effects of colonising bacteria in stable chronic obstructive pulmonary disease. Respir Res, 2014-09-14;15(0):114. 2014-09-14 [PMID: 25218165] (Human)
• Pisetsky D, Trace S, Brownley K, Hamer R, Zucker N, Roux-Lombard P, Dayer J, Bulik C The expression of cytokines and chemokines in the blood of patients with severe weight loss from anorexia nervosa: an exploratory study. Cytokine, 2014-06-14;69(1):110-5. 2014-06-14 [PMID: 25022969] (Human)
• Proenca-Ferreira R, Brugnerotto A, Garrido V, Dominical V, Vital D, Ribeiro M, dos Santos M, Traina F, Olalla-Saad S, Costa F, Conran N Endothelial activation by platelets from sickle cell anemia patients. PLoS ONE, 2014-02-13;9(2):e89012. 2014-02-13 [PMID: 24551209] (Human)
• Pinti M, Cevenini E, Nasi M, De Biasi S, Salvioli S, Monti D, Benatti S, Gibellini L, Cotichini R, Stazi M, Trenti T, Franceschi C, Cossarizza A Circulating mitochondrial DNA increases with age and is a familiar trait: Implications for "inflamm-aging" Eur J Immunol, 2014-02-13;44(5):1552-62. 2014-02-13 [PMID: 24470107] (Human)
• Teichert T, Vossoughi M, Vierkotter A, Sugiri D, Schikowski T, Schulte T, Roden M, Luckhaus C, Herder C, Kramer U Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study. PLoS ONE, 2013-12-10;8(12):e83042. 2013-12-10 [PMID: 24340078] (Human)

Bioinformatics

• Gene Symbol: CXCL8