Human TNF-alpha QuantiGlo ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human TNF-alpha QuantiGlo ELISA Kit Summary

• Background:
  The QuantiGlo Human TNF-alpha Immunoassay is a 5 hour solid phase chemiluminescent ELISA designed to measure human TNF-alpha in cell culture supernates, serum, and plasma. It contains E. coli-derived recombinant human TNF-alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human TNF-alpha . Results ob...tained using naturally occurring TNF-alpha samples showed linear curves that were parallel to the standard curves obtained using the QuantiGlo^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TNF-alpha .
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• Specificity: Natural and recombinant human TNF-alpha .
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TNF-alpha QuantiGlo ELISA Kit

• APC1 protein
• Cachectin
• Cachetin
• DIF
• TNF
• TNF, monocyte-derived
• TNFA
• TNF-A
• TNFalpha
• TNF-alpha
• TNF-alphacachectin
• TNFATNF, macrophage-derived
• TNFG1F
• TNFSF1A
• TNFSF2
• TNFSF2TNF superfamily, member 2
• tumor necrosis factor (TNF superfamily, member 2)
• tumor necrosis factor alpha
• Tumor necrosis factor ligand superfamily member 2
• tumor necrosis factor
• tumor necrosis factor-alpha

Background

Tumor Necrosis Factor alpha (TNF-alpha ), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily (1). It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (2-5). TNF-alpha is also involved in a number of pathological conditions including asthma, Crohn's disease, rheumatoid arthritis, neuropathic pain, obesity, type 2 diabetes, septic shock, autoimmunity, and cancer (5-11). 

 Human TNF-alpha is synthesized as a 26 kDa type II transmembrane protein that consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (12, 13). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus monkey, and 71%-92% aa identity with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha . It is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (14). Cell surface TNF-alpha can both induce the lysis of tumor cells and virus infected cells, and generate its own downstream cell signaling following ligation by soluble TNF RI (15, 16). Shedding of membrane bound TNF-alpha by TACE/ ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (17-19). 

 TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (20, 21) and the hematopoietic cell-restricted 78-80 kDa TNF RII (22, 23), both of which are also expressed as homotrimers (1, 24). Both type I and type II receptors bind TNF-alpha with comparable affinity and can promote NF kappa B activation (25-28). Only TNF RI, however, contains a cytoplasmic death domain which triggers the activation of apoptosis (3, 29). Soluble forms of both types of receptors are released into human serum and urine and can neutralize the biological activity of TNF-alpha (30-32).

Publications for TNF-alpha (QTA00C)(25)

Publications using QTA00C

• Mucientes, A;Lisbona-Montañez, JM;Mena-Vázquez, N;Ruiz-Limón, P;Manrique-Arija, S;García-Studer, A;Ortiz-Márquez, F;Fernández-Nebro, A; Intestinal Dysbiosis, Tight Junction Proteins, and Inflammation in Rheumatoid Arthritis Patients: A Cross-Sectional Study International journal of molecular sciences 2024-08-08 [PMID: 39201334] (Human)
• Du, F;Rische, CH;Li, Y;Vincent, MP;Krier-Burris, RA;Qian, Y;Yuk, SA;Almunif, S;Bochner, BS;Qiao, B;Scott, EA; Controlled adsorption of multiple bioactive proteins enables targeted mast cell nanotherapy Nature nanotechnology 2024-01-16 [PMID: 38228804] (Human)
• Iwahori, K;Nii, T;Yamaguchi, N;Kawasaki, T;Okamura, S;Hashimoto, K;Matsuki, T;Tsujino, K;Miki, K;Osa, A;Goya, S;Abe, K;Mori, M;Takeda, Y;Yamada, T;Kida, H;Kumanogoh, A; A randomized phase 2 study on demeclocycline in patients with mild-to-moderate COVID-19 Scientific reports 2023-08-23 [PMID: 37612352] (Human)
• F Du, C Rische, Y Li, M Vincent, R Krier-Burr, Y Qian, S Yuk, S Almunif, B Bochner, B Qiao, E Scott Bioactive multi-protein adsorption enables targeted mast cell nanotherapy Research square, 2023-01-26;0(0):. 2023-01-26 [PMID: 36747749] (Human)
• I Bortone, R Zupo, F Castellana, S Aresta, L Lampignano, S Sciarra, C Griseta, TA Stallone, G Sborgia, M Lozupone, F Panza, G Lagravines, P Battista, R Sardone Motoric Cognitive Risk Syndrome, Subtypes and 8-Year All-Cause Mortality in Aging Phenotypes: The Salus in Apulia Study Brain sciences, 2022-06-29;12(7):. 2022-06-29 [PMID: 35884669] (Human)
• R Zupo, F Castellana, S De Nucci, G De Pergola, M Lozupone, I Bortone, M Castellana, G Sborgia, L Lampignano, G Giannelli, F Panza, R Sardone Liver Fibrosis and 8-Year All-Cause Mortality Trajectories in the Aging Cohort of the Salus in Apulia Study Biomedicines, 2021-11-04;9(11):. 2021-11-04 [PMID: 34829846] (Human)
• T Tamura, M Suzuki, K Hayashida, Y Kobayashi, J Yoshizawa, T Shibusawa, M Sano, S Hori, J Sasaki Hydrogen gas inhalation alleviates oxidative stress in patients with post-cardiac arrest syndrome J Clin Biochem Nutr, 2020-04-03;67(2):214-221. 2020-04-03 [PMID: 33041520] (Human)
• V Manganelli, A Capozzi, S Truglia, C Alessandri, E Lococo, T Garofalo, C De Carolis, F Conti, G Valesini, M Sorice, A Longo, R Misasi Elevated Serum Level of HMGB1 in Patients with the Antiphospholipid Syndrome J Immunol Res, 2017-12-19;2017(0):4570715. 2017-12-19 [PMID: 29410969] (Human)
• A Yoshihisa, Y Kanno, S Watanabe, T Yokokawa, S Abe, M Miyata, T Sato, S Suzuki, M Oikawa, A Kobayashi, T Yamaki, H Kunii, K Nakazato, H Suzuki, T Ishida, Y Takeishi Impact of nutritional indices on mortality in patients with heart failure Open Heart, 2018-01-09;5(1):e000730. 2018-01-09 [PMID: 29344381] (Human)
• M Minatoya, S Itoh, A Araki, N Tamura, K Yamazaki, C Miyashita, R Kishi Association between Fetal Adipokines and Child Behavioral Problems at Preschool Age: The Hokkaido Study on Environment and Children's Health Int J Environ Res Public Health, 2018-01-11;15(1):. 2018-01-11 [PMID: 29324697] (Human)
• HE Wajdner, J Farrington, C Barnard, PT Peachell, CG Schnackenb, JP Marino, X Xu, K Affleck, M Begg, EP Seward Orai and TRPC channel characterization in Fc?RI-mediated calcium signaling and mediator secretion in human mast cells Physiol Rep, 2017-03-01;5(5):. 2017-03-01 [PMID: 28292887] (Human)
• Kawamura T, Umemura T, Umegaki H, Imamine R, Kawano N, Tanaka C, Kawai M, Minatoguchi M, Kusama M, Kouchi Y, Watarai A, Kanai A, Nakashima E, Hotta N Effect of renal impairment on cognitive function during a 3-year follow up in elderly patients with type 2 diabetes: Association with microinflammation. J Diabetes Investig, 2014-02-04;5(5):597-605. 2014-02-04 [PMID: 25411629] (Human)
• Gao H, Mejhert N, Fretz J, Arner E, Lorente-Cebrian S, Ehrlund A, Dahlman-Wright K, Gong X, Stromblad S, Douagi I, Laurencikiene J, Dahlman I, Daub C, Ryden M, Horowitz M, Arner P Early B cell factor 1 regulates adipocyte morphology and lipolysis in white adipose tissue. Cell Metab, 2014-05-22;19(6):981-92. 2014-05-22 [PMID: 24856929] (Human)
• Cifuentes M, Fuentes C, Tobar N, Acevedo I, Villalobos E, Hugo E, Ben-Jonathan N, Reyes M Calcium sensing receptor activation elevates proinflammatory factor expression in human adipose cells and adipose tissue. Mol Cell Endocrinol, 2012-03-16;361(1):24-30. 2012-03-16 [PMID: 22449852] (Human)
• Narla A, Dutt S, McAuley JR Dexamethasone and lenalidomide have distinct functional effects on erythropoiesis. Blood, 2011-04-28;118(8):2296-304. 2011-04-28 [PMID: 21527522] (Human)
• Dod HS, Bhardwaj R, Sajja V, Weidner G, Hobbs GR, Konat GW, Manivannan S, Gharib W, Warden BE, Nanda NC, Beto RJ, Ornish D, Jain AC Effect of intensive lifestyle changes on endothelial function and on inflammatory markers of atherosclerosis. Am. J. Cardiol., 2010-02-01;105(3):362-7. 2010-02-01 [PMID: 20102949] (Human)
• Ziesche E, Scheiermann P, Bachmann M, Sadik CD, Hofstetter C, Zwissler B, Pfeilschifter J, Muhl H Dexamethasone suppresses interleukin-22 associated with bacterial infection in vitro and in vivo. Clin. Exp. Immunol., 2009-09-01;157(3):370-6. 2009-09-01 [PMID: 19664145] (Human)
• Challier JC, Basu S, Bintein T, Minium J, Hotmire K, Catalano PM, Hauguel-De Mouzon S Obesity in pregnancy stimulates macrophage accumulation and inflammation in the placenta. Placenta, 2008-02-11;29(3):274-81. 2008-02-11 [PMID: 18262644] (Human)
• Hu P, Han Z, Couvillon AD, Kaufman RJ, Exton JH Autocrine tumor necrosis factor alpha links endoplasmic reticulum stress to the membrane death receptor pathway through IRE1alpha-mediated NF-kappaB activation and down-regulation of TRAF2 expression. Mol. Cell. Biol., 2006-04-01;26(8):3071-84. 2006-04-01 [PMID: 16581782] (Human)
• Tsai MY, Hanson NQ, Straka RJ, Hoke TR, Ordovas JM, Peacock JM, Arends VL, Arnett DK Effect of influenza vaccine on markers of inflammation and lipid profile. J. Lab. Clin. Med., 2005-06-01;145(6):323-7. 2005-06-01 [PMID: 15976761] (Human)
• Matsuda M, Kawasaki F, Inoue H, Kanda Y, Yamada K, Harada Y, Saito M, Eto M, Matsuki M, Kaku K Possible contribution of adipocytokines on diabetic neuropathy. Diabetes Res. Clin. Pract., 2004-12-01;66(0):S121-3. 2004-12-01 [PMID: 15563961] (Human)
• Nishishita T, Ouchi K, Zhang X, Inoue M, Inazawa T, Yoshiura K, Kuwabara K, Nakaoka T, Watanabe N, Igura K, Takahashi TA, Yamashita N A potential pro-angiogenic cell therapy with human placenta-derived mesenchymal cells. Biochem. Biophys. Res. Commun., 2004-12-03;325(1):24-31. 2004-12-03 [PMID: 15522196] (Human)
• Birdsall HH, Porter WJ, Green DM, Rubio J, Trial J, Rossen RD Impact of fibronectin fragments on the transendothelial migration of HIV-infected leukocytes and the development of subendothelial foci of infectious leukocytes. J. Immunol., 2004-08-15;173(4):2746-54. 2004-08-15 [PMID: 15294993] (Human)
• Kuyvenhoven JP, Molenaar IQ, Verspaget HW, Veldman MG, Palareti G, Legnani C, Moolenburgh SE, Terpstra OT, Lamers CB, van Hoek B, Porte RJ Plasma MMP-2 and MMP-9 and their inhibitors TIMP-1 and TIMP-2 during human orthotopic liver transplantation. The effect of aprotinin and the relation to ischemia/reperfusion injury. Thromb. Haemost., 2004-03-01;91(3):506-13. 2004-03-01 [PMID: 14983226] (Human)
• Dahaba AA, Elawady GA, Rehak PH, List WF Procalcitonin and proinflammatory cytokine clearance during continuous venovenous haemofiltration in septic patients. Anaesth Intensive Care, 2002-06-01;30(3):269-74. 2002-06-01 [PMID: 12075632] (Human)

FAQs for TNF-alpha (QTA00C). (Showing 1 - 4 of 4 FAQs).

1. I need to know about TNF-alpha antibody kits for mice.  ..show answer..
   • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.  ..show answer..
   • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?  ..show answer..
   • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?  ..show answer..
   • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.