Hyaluronan synthase 2 Antibody (4E7) - BSA Free

Western Blot: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Western blot analysis using HAS2 mouse mAb against NTERA-2 (1), HEK293 (2) cell lysate.

Western Blot: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Western blot analysis using HAS2 mouse mAb against NTERA-2 (1), HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Immunofluorescence analysis of HeLa cells using HAS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Analysis of ovarian cancer tissues using HAS2 mouse mAb with DAB staining.

Western Blot: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Western blot analysis using HAS2 mAb against human HAS2 recombinant protein. (Expected MW is 37.5 kDa)

ELISA: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Hyaluronan synthase 2 Antibody (4E7) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Hyaluronan synthase 2 (AA: 67-170) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HAS2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 63.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Hyaluronan synthase 2 Antibody (4E7) - BSA Free

• EC 2.4.1.212
• HA synthase 2
• hyaluronan synthase 2
• Hyaluronate synthase 2
• Hyaluronic acid synthase 2
• MGC126241
• MGC126242

Background

The HAS2 gene encodes a 552 amino acid long, 63 kDA hyaluronan synthase 2 protein that functions in hyaluronan/hyaluronic acid (HA) synthesis which is critical to the extracellular matrix. HAS2 is involved in hyaluronan metabolism, MPSII (Hunter syndrome), and MPS VI (Maroteaux-Lamy syndrome). HAS2 is also linked to arthropathy, fibrosarcoma, endometrial cancer, osteoarthritis, myeloma, benign tumors, breast cancer, ovarian cancer, and colon carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hyaluronan synthase 2 Antibody (NBP2-37446)(2)

Publications using NBP2-37446

• Fang Liu, Zhun Liu, Weisheng Cheng, Qingquan Zhao, Xinyu Zhang, He Zhang, Miao Yu, He Xu, Yichen Gao, Qianrui Jiang, Guojun Shi, Likun Wang, Shanshan Gu, Jia Wang, Nan Cao, Zhongyan Chen The PERK Branch of the Unfolded Protein Response Safeguards Protein Homeostasis and Mesendoderm Specification of Human Pluripotent Stem Cells. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023-12-22 [PMID: 37890465]
• Gomi T, Imamura T. Comprehensive histological investigation of age-related changes in dermal extracellular matrix and muscle fibers in the upper lip vermilion International Journal of Cosmetic Science 2020-08-01 [PMID: 32274802]

Bioinformatics

• Gene Symbol: HAS2