Mouse ICAM-1/CD54 ELISA Kit (Colorimetric)

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Summary
Product Discontinued
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    • Catalog Number
      KA0345
    • Availability
      Product Discontinued

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Mouse ICAM-1/CD54 ELISA Kit (Colorimetric) Summary

Standard Curve Range
156 to 10000 pg/mL
Sensitivity
10 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 6.3%
Intra-Assay
CV% < 5.4%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ICAM1

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Icam1 (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse Icam1.
Publications
Read Publication using KA0345.

Reactivity Notes

Mouse

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 96-well plate precoated with anti-mouse ICAM-1 antibody
  2. ABC diluent buffer
  3. Adhesive cover
  4. Antibody diluent buffer
  5. Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
  6. Biotinylated anti-mouse ICAM-1 antibody, dilution 1:100
  7. Lyophilized recombinant mouse ICAM-1 standard
  8. Sample diluent buffer
  9. TMB color developing agent
  10. TMB stop solution

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mouse ICAM-1/CD54 ELISA Kit (Colorimetric)

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ICAM1