IFN-alpha/beta R2 Antibody [Unconjugated]

Recombinant Mouse Limitin (1535-LM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse Limitin (30 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IFN-a/ beta R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1083). The ND50 is typically 2-10 µg/mL.

A20 cells were stained with Goat Anti-Mouse IFN‑ alpha / beta R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1083, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: WB, Flow, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IFN-alpha/beta R2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IFN-alpha / beta R2
  Ser22-Ala239 (Lys160Asn)
  Accession # Q9D1R7
• Specificity: Detects mouse IFN‑ alpha / beta R2 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) IFN-gamma R2 and rmIL-20 R alpha is observed, 5% cross-reactivity with recombinant human (rh) IL-10 R beta , rmIFN-gamma RI, and rhIL-20 R beta is observed and less than 1% cross-reactivity with rhIFN-beta R alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IFNAR2
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells
  • Neutralization 2-10 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-alpha/beta R2 Antibody [Unconjugated]

• beta receptor10
• human interferon alpha
• human interferon alpha/beta receptor
• IFNABR
• IFN-alpha binding protein
• IFN-alpha/beta R2
• IFN-alpha/beta receptor 2
• IFN-alpha-REC
• IFNAR2
• IFN-aR2
• IFNARB
• IFNbR2
• IFN-bR2
• IFN-R
• IFN-R-2
• interferon (alpha, beta and omega) receptor 2
• Interferon alpha binding protein
• interferon alpha/beta receptor 2
• interferon-alpha/beta receptor beta chain
• Type I interferon receptor 2

Background

IFN-alpha / beta R2 belongs to the type II cytokine receptor family. It complexes with IFN-alpha / beta R1 to form the signaling receptor complex for the family of alpha and beta IFN subtypes. By alternative splicing, IFN-alpha / beta R2 can exist as a secreted soluble protein or as a type I membrane protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-alpha/beta R2 Antibody (AF1083)(6)

Publications using AF1083

• Junling Ren, Xiao Han, Hannah Lohner, Rosalie G. Hoyle, Jiong Li, Shuang Liang et al. P. gingivalis Infection Upregulates PD-L1 Expression on Dendritic Cells, Suppresses CD8+ T-cell Responses, and Aggravates Oral Cancer Cancer Immunology Research 3/1/2023 [PMID: 36633576]
• AF Lloyd, CL Davies, RK Holloway, Y Labrak, G Ireland, D Carradori, A Dillenburg, E Borger, D Soong, JC Richardson, T Kuhlmann, A Williams, JW Pollard, A des Rieux, J Priller, VE Miron Central nervous system regeneration is driven by microglia necroptosis and repopulation Nat. Neurosci., 2019-06-10;0(0):. 2019-06-10 [PMID: 31182869] (Neutralization, Mouse)
• Chengfei Zhang, Yan Yan, Hongwang He, Li Wang, Na Zhang, Jie Zhang et al. IFN-stimulated P2Y13 protects mice from viral infection by suppressing the cAMP/EPAC1 signaling pathway Journal of Molecular Cell Biology 5/1/2019 [PMID: 30137373]
• C Zhang, H He, L Wang, N Zhang, H Huang, Q Xiong, Y Yan, N Wu, H Ren, H Han, M Liu, M Qian, B Du Virus-Triggered ATP Release Limits Viral Replication through Facilitating IFN-? Production in a P2X7-Dependent Manner J. Immunol., 2017-07-07;0(0):. 2017-07-07 [PMID: 28687662] (Neutralization, Mouse)
• Ruimei Li, Binghe Tan, Yan Yan, Xiaobin Ma, Na Zhang, Zhi Zhang et al. Extracellular UDP and P2Y6 Function as a Danger Signal To Protect Mice from Vesicular Stomatitis Virus Infection through an Increase in IFN-beta Production The Journal of Immunology 11/1/2014 [PMID: 25261483]
• Zhang F, Lee J, Wang D, Dong Z Differential Growth of IFN-beta-Engineered Tumor Cells in Nude and IFN Receptor-Null Mice. J. Interferon Cytokine Res., 2006-02-01;26(2):108-18. 2006-02-01 [PMID: 16487031] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: IFNAR2
• Uniprot:
  • Q9D1R7()