IFN-alpha/beta R2 Antibody - BSA Free

Western Blot: IFN-alpha/beta R2 Antibody [NBP1-31665] - ORF54 induces degradation of IFNAR1.24 hours post transfection, 293T cells were treated with 1 ug/mL of puromycin to select for transfected cells. 48 hours post treatment, cells overexpressing vector control or FLAG-tagged MHV-68 ORFs were: Upper panels: Lysed to analyze level of IFNAR1. The membrane was subsequently stripped to demonstrate equal expression of the FLAG-ORFs and Beta-actin as controls, and IGF1Beta to demonstrate specificity of ORF54 to IFNAR1. Lower panels: The same lysates were also subjected to immunoblot against IFNAR2. The membrane was stripped twice to demonstrate equal expression of FLAG-ORFs and Beta-actin as controls. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1002292) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: IFN-alpha/beta R2 Antibody [NBP1-31665] - Analysis of methanol-fixed Hep3B.

Western Blot: IFN-alpha/beta R2 Antibody [NBP1-31665] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IFN-alpha/beta R2 antibody (NBP1-31665) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IFN-alpha/beta R2 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human IFN-alpha/beta R2. The exact sequence is proprietary.
• Localization: Membrane, Single-pass type I membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IFNAR2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for IFN-alpha/beta R2 Antibody - BSA Free

• beta receptor10
• human interferon alpha
• human interferon alpha/beta receptor
• IFNABR
• IFN-alpha binding protein
• IFN-alpha/beta R2
• IFN-alpha/beta receptor 2
• IFN-alpha-REC
• IFNAR2
• IFN-aR2
• IFNARB
• IFNbR2
• IFN-bR2
• IFN-R
• IFN-R-2
• interferon (alpha, beta and omega) receptor 2
• Interferon alpha binding protein
• interferon alpha/beta receptor 2
• interferon-alpha/beta receptor beta chain
• Type I interferon receptor 2

Background

The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Multiple transcript variants encoding at least two different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for IFN-alpha/beta R2 Antibody (NBP1-31665)(1)

Publication using NBP1-31665

• Leang RS, Wu T-T, Hwang S et al. The Anti-interferon Activity of Conserved Viral dUTPase ORF54 is Essential for an Effective MHV-68 Infection. PLoS Pathog;7(10). 2011-10-01 [PMID: 21998588]

Bioinformatics

• Gene Symbol: IFNAR2
• Entrez:
  • 3455(Human)
• Uniprot:
  • P48551(Human)