IFN-gamma Antibody (JM10-10)

Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Human liver tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Human liver tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Analysis of Hela cells with Interferon gamma antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Mouse liver tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Human liver carcinoma tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: JM10-10
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

IFN-gamma Antibody (JM10-10) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within N-terminal human IFN-gamma. (SwissProt: P01579 Human; SwissProt: P01580 Mouse; SwissProt: P01581 Rat)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IFNG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500
• Application Notes: Use in ICC/IF reported in scientific literature (PMID:33491667).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IFN-gamma Antibody (JM10-10)

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

IFN-gamma, a type II interferon and an acid-labile interferon, is mainly produced by CD4 and CD8 T-lymphocytes and natural killer cells activated by antigens, mitogens or alloantigens. IFN-gamma shows no significant homology with IFN-beta or IFN-alpha. IFN-gamma has antiviral and antiparasitic activities and exhibits also immunoregulatory and proinflammatory activities. IFN-gamma induces cytokine production and promotes antigen presentation by up regulating the expression of MHC proteins as well as other antigen proteins. In contrast to other interferons, which are mostly antiviral, the main activity of IFN-gamma seems to be immunomodulatory. Human IFN-gamma is species-specific and is only biologically active in human and primate cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-gamma Antibody (NBP2-66900)(6)

Publications using NBP2-66900

• Han SJ, Jain P, Gilad Y et al. Steroid Receptor Coactivator-3 is a Key Modulator of Regulatory T Cell-Mediated Tumor Evasion bioRxiv : the preprint server for biology 2023-03-29 [PMID: 37034717]
• Huang HC, Wang SH, Fang GC et al. Upregulation of PD-L1 by SARS-CoV-2 promotes immune evasion Journal of medical virology 2023-02-01 [PMID: 36609964] (Immunocytochemistry/ Immunofluorescence)
• Han SJ, Sung N, Wang J et al. Steroid receptor coactivator-3 inhibition generates breast cancer antitumor immune microenvironment Breast cancer research : BCR 2022-10-31 [PMID: 36316775] (IHC-P, Mouse)
  
  Details:
  Dilution used in IHC 1:300
• Perdigoto AL, Deng S, Du KC et al. Immune cells and their inflammatory mediators modify beta cells and cause checkpoint inhibitor-induced diabetes JCI insight 2022-08-04 [PMID: 35925682] (IHC-P, Mouse, Human)
• Meng J, Peng J, Feng J Et al. Niraparib exhibits a synergistic anti-tumor effect with PD-L1 blockade by inducing an immune response in ovarian cancer Journal of translational medicine 2021-10-07 [PMID: 34620163] (IHC-P, Human)
• Aftabizadeh M, Li YJ, Zhao Q, et al. Potent antitumor effects of cell-penetrating peptides targeting STAT3 axis JCI insight 2021-01-25 [PMID: 33491667] (ICC/IF)

FAQs for IFN-gamma Antibody (NBP2-66900). (Showing 1 - 1 of 1 FAQs).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?  ..show answer..
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG