IFN-gamma R1/CD119 Antibody (062) - Azide and BSA Free

Flow Cytometry: IFN-gamma R1/CD119 Antibody (062) [NBP2-90685] - Flow cytometric analysis of Mouse IFNGR1(CD119) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse IFNGR1(CD119), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

ICC/IF- IFN-gamma R1/CD119 Antibody (062) [NBP2-90685] -staining of mouse IFNGR1 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse IFNGR1 monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).

• Reactivity: Mu
• Applications: Flow, ICC/IF, ELISA
• Clone: 062
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

IFN-gamma R1/CD119 Antibody (062) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse IFN-gamma R1/CD119 (Uniprot#: P15261; Met1-Asp253).
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IFNGR1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:25-1:100
  • Immunocytochemistry/ Immunofluorescence 1:20-1:100
  • Sandwich ELISA Capture 1:250-1:2000
• Application Notes: This antibody will detect Human IFN-gamma R1/CD119 in ELISA pair set (Catalog: # NBP2-79602).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for IFN-gamma R1/CD119 Antibody (062) - Azide and BSA Free

• AVP, type 2
• CD119 antigen
• CD119
• FLJ45734
• IFN-gamma R1
• IFN-gamma receptor 1
• IFNgammaR1
• IFN-gamma-R1
• IFNGR
• IFNGR1
• IFN-gR1
• immune interferon receptor 1
• interferon gamma receptor 1
• interferon-gamma receptor alpha chain
• type 2

Background

IFN gamma receptor beta is part of the receptor for interferon gamma. This class II cytokine receptor pairs with CDw119 to form the IFN gamma receptor and is an integral part of the IFN gamma signal transduction pathway. CDw119 serves as the IFN gamma binding chain and associates with the IFN gamma beta chain which is required for receptor signaling. The extracellular portion of both the IFN gamma receptor alpha and beta chains must be species matched. The IFN gamma receptor beta chain is expressed on T and B cells, NK cells, monocytes/ macrophages, and fibroblasts. Binding of IFN gamma induces receptor dimerization, internalization, Jak1 and Jak2 protein kinase activation and, ultimately, STAT1 activation. It is also likely to interact with GAF. IFN gamma initiates and regulates a variety of immune responses and is required for signal transduction. Contains 2 fibronectin type III domains. Defects in IFN gamma Receptor beta are a cause of mendelian susceptibility to mycobacterial disease (MSMD), a rare condition that confers predisposition to illness caused by several mycobacteria strains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IFNGR1