Goat anti-Mouse IgG Secondary Antibody [HRP Polymer] (IHC-Prediluted)

Alkaline Phosphatase was detected in paraffin-embedded sections of human liver cancer using Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) at 1 μg/mL for 1 hour at room temperature followed byincubation for 30 minutes at room temperature with Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Tissue was stained with DAB (brown color) andcounterstained with hematoxylin (blue color).

Alkaline Phosphatase was detected in paraffin-embedded sections of human liver cancer using Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) at 1 μg/mL for 1 hour at room temperature followed byincubation for 30 minutes at room temperature with Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Tissue was stained with DAB (brown color) andcounterstained with hematoxylin (blue color).

• Reactivity: Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: HRP Polymer
• Format: IHC-Prediluted

Goat anti-Mouse IgG Secondary Antibody [HRP Polymer] (IHC-Prediluted) Summary

• Immunogen: Mouse IgG
• Specificity: Detects heavy ( gamma ) chains on mouse IgG and light chains on all mouse immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin mouse serum proteins or serum proteins from bovine, horse, human, pig or rabbit, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: 6 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied as a solution in PBS with preservative.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat anti-Mouse IgG Secondary Antibody [HRP Polymer] (IHC-Prediluted)

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Background

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (VC001)(7)

Publications using VC001

• Kobayashi, H;Iida, T;Ochiai, Y;Malagola, E;Zhi, X;White, RA;Qian, J;Wu, F;Waterbury, QT;Tu, R;Zheng, B;LaBella, JS;Zamechek, LB;Ogura, A;Woods, SL;Worthley, DL;Enomoto, A;Wang, TC; Neuro-mesenchymal interaction mediated by a ?2 adrenergic-nerve growth factor feedforward loop promotes colorectal cancer progression Cancer discovery 2024-08-13 [PMID: 39137067] (Immunohistochemistry, Mouse)
• Wei F, Tuong ZK, Omer M et al. A novel multifunctional radioprotective strategy using P7C3 as a countermeasure against ionizing radiation-induced bone loss Bone research 2023-06-29 [PMID: 37385982]
• F Wei, CJ Neal, TS Sakthivel, Y Fu, M Omer, A Adhikary, S Ward, KM Ta, S Moxon, M Molinari, J Asiatico, M Kinzel, SN Yarmolenko, V San Cheong, N Orlovskaya, R Ghosh, S Seal, M Coathup A novel approach for the prevention of ionizing radiation-induced bone loss using a designer multifunctional cerium oxide nanozyme Bioactive materials, 2022-09-21;21(0):547-565. 2022-09-21 [PMID: 36185749]
• Zhen Lu, Weiqun Mao, Hailing Yang, Janice M. Santiago-O’Farrill, Philip J. Rask, Jayanta Mondal et al. SIK2 inhibition enhances PARP inhibitor activity synergistically in ovarian and triple-negative breast cancers Journal of Clinical Investigation 2022-06-01 [PMID: 35642638]
• TC Kan, MH Lin, CC Cheng, JW Lu, MT Sheu, YS Ho, S Rahayu, J Chang Preclinical Therapeutic Assessment of a New Chemotherapeutics [Dichloro(4,4'-Bis(2,2,3,3-Tetrafluoropropoxy) Methyl)-2,2'-Bipryridine) Platinum] in an Orthotopic Patient-Derived Xenograft Model of Triple-Negative Breast Cancers Pharmaceutics, 2022-04-11;14(4):. 2022-04-11 [PMID: 35456673] (IHC, Mouse)
• PT Tran, N Asghar, M Johansson, W Melik Roles of the Endogenous Lunapark Protein during Flavivirus Replication Viruses, 2021-06-22;13(7):. 2021-06-22 [PMID: 34206552] (ICC, Human)
• Hatem Rouatbi, Nesrine Farhat, Ruth Heying, Arlette Gérard, Jaime F. Vazquez-Jimenez, Marie-Christine Seghaye Right Atrial Myocardial Remodeling in Children With Atrial Septal Defect Involves Inflammation, Growth, Fibrosis, and Apoptosis Frontiers in Pediatrics 2020-02-14 [PMID: 32117843]