Mouse IgG ELISA Kit (Colorimetric)

ELISA: Mouse IgG ELISA Kit (Colorimetric) [NBP2-60062] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ELISA: Mouse IgG ELISA Kit (Colorimetric) [NBP2-60062] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Mouse IgG ELISA Kit (Colorimetric) [NBP2-60062] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell culture supernates, urine and tissue homogenates
• Standard Curve Range: 125 ng/mL - 8000 ng/mL
• Sensitivity: 29 ng/mL

Mouse IgG ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse IgG. No significant cross-reactivity or interference between mouse IgG and analogues was observed.
• Standard Curve Range: 125 ng/mL - 8000 ng/mL
• Sensitivity: 29 ng/mL
• Assay Type: Competitive ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IGHG1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse IgG ELISA Kit (Colorimetric)

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with mouse IgG. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for mouse IgG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of mouse IgG in samples. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for IgG ELISA Kit (NBP2-60062)(1)

Publication using NBP2-60062

• Xu X, Sui B, Liu X, Sun J Superior low-immunogenicity of tilapia type I collagen based on unique secondary structure with single calcium binding motif over terrestrial mammals by inhibiting activation of DC intracellular Ca2+-mediated STIM1-Orai1/NF-kappa B pathway Materials Science and Engineering: C 2021-01-01 [PMID: 34857289] (ELISA)

Bioinformatics

• Gene Symbol: IGHG1